Embryonic stem （ES） cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy.The main catalyst for ES c...Embryonic stem （ES） cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy.The main catalyst for ES cells differentiation is the growth into embryoid bodies （EBs）,which are utilized widely as the trigger of in vitro differentiation.In this study,a novel method for generating EBs from mouse ES cells through culture in collagen/Matrigel scaffolds was successfully established.When single ES cells were seeded in three dimensional collagen/Matrigel scaffolds,they grew into aggregates gradually and formed simple EBs with circular structures.After 7 days＇ culture,they formed into cystic EBs that would eventually differentiate into the three embryonic germ layers.Evaluation of the EBs in terms of morphology and potential to differentiate indicated that they were typical in structure and could generate various cell types;they were also able to form into tissue-like structures.Moreover,with introduction of ascorbic acid,ES cells differentiated into cardiomyocytes efficiently and started contracting synchronously at day 19.The results demonstrated that collagen/Matrigel scaffolds supported EBs formation and their subsequent differentiation in a single three dimensional environment.展开更多
文摘目的以肿瘤坏死因子-α(TNF-α)诱导的人脐静脉内皮细胞(HUVEC)为研究对象,探讨罗仙子(Musca Domestica)抗炎活性部位在体外对HUVEC增殖及Matrigel小管形成的影响。方法将构建的HUVEC细胞分为正常对照组、模型组及罗仙子活性部位处理组,制备罗仙子抗炎活性部位,采用流式细胞术结合ModFit LT软件分析,明确罗仙子抗炎活性部位对HUVEC增殖的影响;采用Matrigel小管形成实验结合Image Pro Plus软件分析,明确罗仙子抗炎活性部位对HUVEC血管形成的影响。结果相对模型组,罗仙子抗炎活性部位处理组HUVEC增殖得到显著的抑制,增殖系数降低明显(P<0.01),同时,该处理组Matrigel小管形成的数量、面积和长度显著减少(P<0.01)。结论罗仙子抗炎活性部位对TNF-α诱导HUVEC增殖及Matrigel小管形成具有抑制作用。
基金Supported by The Shanghai Municipal Natural Science Foundation,No.11ZR1405500the Shanghai Municipal Science and Technology Commission grant,No.13140902401
文摘AIM: To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma.
基金supported by the National High Technology Research and Development Program of China (No 2006AA02A105 to CW)the National Nature Science Foundation of China (No 30530220)Beijing Nature Science Foundation of China (No 7062053)
文摘Embryonic stem （ES） cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy.The main catalyst for ES cells differentiation is the growth into embryoid bodies （EBs）,which are utilized widely as the trigger of in vitro differentiation.In this study,a novel method for generating EBs from mouse ES cells through culture in collagen/Matrigel scaffolds was successfully established.When single ES cells were seeded in three dimensional collagen/Matrigel scaffolds,they grew into aggregates gradually and formed simple EBs with circular structures.After 7 days＇ culture,they formed into cystic EBs that would eventually differentiate into the three embryonic germ layers.Evaluation of the EBs in terms of morphology and potential to differentiate indicated that they were typical in structure and could generate various cell types;they were also able to form into tissue-like structures.Moreover,with introduction of ascorbic acid,ES cells differentiated into cardiomyocytes efficiently and started contracting synchronously at day 19.The results demonstrated that collagen/Matrigel scaffolds supported EBs formation and their subsequent differentiation in a single three dimensional environment.
