Selecting probiotic strains from maternal and infant sources,combined with processes tailored for early-life applications,may enhance probiotic efficacy.In this study,26 Bifidobacterium strains(five species)isolated f...Selecting probiotic strains from maternal and infant sources,combined with processes tailored for early-life applications,may enhance probiotic efficacy.In this study,26 Bifidobacterium strains(five species)isolated from infant and maternal feces,and breast milk were systematically assessed for physiological traits,techno-logical potential,functionality,and safety.Distinct source-and species-specific differences were observed.Breast milk-derived strains showed lower tolerance to simulated gastric fluid than those from feces.Regarding anti-biotic susceptibility,B.longum subsp.longum was sensitive to tetracycline and streptomycin but resistant to erythromycin,while B.breve showed the opposite profile.A comprehensive evaluation model integrating nine assessment parameters identified one B.breve and one B.longum subsp.longum strain with superior potential for application.Correlation analyses revealed significant relationships among physiological traits and between traits and functional genes.For instance,antibacterial activity and organic acid production showed positive correla-tions with each other,as well as with genes encoding specific virulence factors and transport proteins.These findings offer mechanistic and genomic insights for selecting probiotics tailored to early-life health.展开更多
The aim of this study was to investigate the clinical characteristics of neonates born to SARS-CoV-2 infected mothers and increase the current knowledge on the perinatal consequences of COVID-19.Nineteen neonates were...The aim of this study was to investigate the clinical characteristics of neonates born to SARS-CoV-2 infected mothers and increase the current knowledge on the perinatal consequences of COVID-19.Nineteen neonates were admitted to Tongji Hospital from January 31 to February 29,2020.Their mothers were clinically diagnosed or laboratory-confirmed with COVID-19.We prospectively collected and analyzed data of mothers and infants.There are 19 neonates included in the research.Among them,10 mothers were confirmed COVID-19 by positive SARS-CoV-2 RT-PCR in throat swab,and 9 mothers were clinically diagnosed with COVID-19.Delivery occurred in an isolation room and neonates were immediately separated from the mothers and isolated for at least 14 days.No fetal distress was found.Gestational age of the neonates was 38.61.5 weeks,and average birth weight was 3293425 g.SARS-CoV-2 RT-PCR in throat swab,urine,and feces of all neonates were negative.SARS-CoV-2 RT-PCR in breast milk and amniotic fluid was negative too.None of the neonates developed clinical,radiologic,hematologic,or biochemical evidence of COVID-19.No vertical transmission of SARS-CoV-2 and no perinatal complications in the third trimester were found in our study.The delivery should occur in isolation and neonates should be separated from the infected mothers and care givers.展开更多
基金supported by the National Key Technologies Research and Development Program[No.2022YFD2100703]the National Natural Science Foundation of China[No.32021005 and 32122067]+1 种基金supported by the Fundamental Research Funds for the Central Universities JUSRP622013Collaborative innovation center of food safety and quality control in Jiangsu Province.
文摘Selecting probiotic strains from maternal and infant sources,combined with processes tailored for early-life applications,may enhance probiotic efficacy.In this study,26 Bifidobacterium strains(five species)isolated from infant and maternal feces,and breast milk were systematically assessed for physiological traits,techno-logical potential,functionality,and safety.Distinct source-and species-specific differences were observed.Breast milk-derived strains showed lower tolerance to simulated gastric fluid than those from feces.Regarding anti-biotic susceptibility,B.longum subsp.longum was sensitive to tetracycline and streptomycin but resistant to erythromycin,while B.breve showed the opposite profile.A comprehensive evaluation model integrating nine assessment parameters identified one B.breve and one B.longum subsp.longum strain with superior potential for application.Correlation analyses revealed significant relationships among physiological traits and between traits and functional genes.For instance,antibacterial activity and organic acid production showed positive correla-tions with each other,as well as with genes encoding specific virulence factors and transport proteins.These findings offer mechanistic and genomic insights for selecting probiotics tailored to early-life health.
文摘The aim of this study was to investigate the clinical characteristics of neonates born to SARS-CoV-2 infected mothers and increase the current knowledge on the perinatal consequences of COVID-19.Nineteen neonates were admitted to Tongji Hospital from January 31 to February 29,2020.Their mothers were clinically diagnosed or laboratory-confirmed with COVID-19.We prospectively collected and analyzed data of mothers and infants.There are 19 neonates included in the research.Among them,10 mothers were confirmed COVID-19 by positive SARS-CoV-2 RT-PCR in throat swab,and 9 mothers were clinically diagnosed with COVID-19.Delivery occurred in an isolation room and neonates were immediately separated from the mothers and isolated for at least 14 days.No fetal distress was found.Gestational age of the neonates was 38.61.5 weeks,and average birth weight was 3293425 g.SARS-CoV-2 RT-PCR in throat swab,urine,and feces of all neonates were negative.SARS-CoV-2 RT-PCR in breast milk and amniotic fluid was negative too.None of the neonates developed clinical,radiologic,hematologic,or biochemical evidence of COVID-19.No vertical transmission of SARS-CoV-2 and no perinatal complications in the third trimester were found in our study.The delivery should occur in isolation and neonates should be separated from the infected mothers and care givers.
