For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility （TGMS） is expressed when the ...For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility （TGMS） is expressed when the plants are grown in conditions with higher or lower critical temperatures. To transfer tgms gene（s） control ing TGMS to Thai rice cultivars by backcross breeding method, a male sterile line was used as a donor parent while Thai rice cultivars ChaiNat 1, PathumThani 1, and SuphanBuri 1 were used as recurrent parents. The BC2F2 lines were developed from backcrossing and selfing. Moreover, the simple sequence repeat （SSR） markers were developed for identifying tgms gene and the linked marker was used for assisting selection in backcrossing. The identification lines were confirmed by pol en observation. The results showed the success of introgression of the tgms gene into Thai rice cultivars. These lines will be tested for combining ability and used as female parent in hybrid rice production in Thailand.展开更多
Watermelon fruit flesh displays various colors.Although genetic loci underlying these variations are identified,the molecular mechanism remains elusive.Here,we assembled a chromosome-scale reference genome of an elite...Watermelon fruit flesh displays various colors.Although genetic loci underlying these variations are identified,the molecular mechanism remains elusive.Here,we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated genetic maps using single nucleotide polymorphism(SNP)and structural variation markers.Several key genetic varients for fruit shape and flesh color were identified.Two variants associated with flesh color were further studied,including one copy number variant(CNV,a triplicate of 1.2 kb DNA)in the promoter region of REDUCED CHLOROPLAST COVERAGE 2(ClREC2)and one SNP in Lycopeneβ-Cyclase(ClLCYB)coding region.These two variants together explained 99.7%of the flesh color variations in 314 watermelon accessions.The SNP in ClLCYB was the same as previously reported,disrupting ClLCYB function.The CNV could strongly enhance ClREC2 expression,consequently increasing the expression of carotenoid biosynthesis genes,the number of plastoglobules within chromoplasts,and carotenoid level in mature fruit flesh.Finally,we proposed a“two-switch”genetic model by integrating two major causative loci,which can explain the formation of the four main flesh colors in different watermelon accessions.These results provide new insights into the regulation of carotenoid biosynthesis and color formation in plants.展开更多
文摘For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility （TGMS） is expressed when the plants are grown in conditions with higher or lower critical temperatures. To transfer tgms gene（s） control ing TGMS to Thai rice cultivars by backcross breeding method, a male sterile line was used as a donor parent while Thai rice cultivars ChaiNat 1, PathumThani 1, and SuphanBuri 1 were used as recurrent parents. The BC2F2 lines were developed from backcrossing and selfing. Moreover, the simple sequence repeat （SSR） markers were developed for identifying tgms gene and the linked marker was used for assisting selection in backcrossing. The identification lines were confirmed by pol en observation. The results showed the success of introgression of the tgms gene into Thai rice cultivars. These lines will be tested for combining ability and used as female parent in hybrid rice production in Thailand.
基金support from the National Key R&D Program of China(2016YFD0100204-26)the Agricultural Science and Technology Innovation Program of China(CAAS-ASTIP-2022-ZFRI)+1 种基金the Special Protection and Utilization of the Crop Germplasm Resources of Chinathe National R&D Infrastructure and Facility Development Program of China and the Scientific Institution Basal Research Fund of ZFRI,CAAS(No.ZGS202109).
文摘Watermelon fruit flesh displays various colors.Although genetic loci underlying these variations are identified,the molecular mechanism remains elusive.Here,we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated genetic maps using single nucleotide polymorphism(SNP)and structural variation markers.Several key genetic varients for fruit shape and flesh color were identified.Two variants associated with flesh color were further studied,including one copy number variant(CNV,a triplicate of 1.2 kb DNA)in the promoter region of REDUCED CHLOROPLAST COVERAGE 2(ClREC2)and one SNP in Lycopeneβ-Cyclase(ClLCYB)coding region.These two variants together explained 99.7%of the flesh color variations in 314 watermelon accessions.The SNP in ClLCYB was the same as previously reported,disrupting ClLCYB function.The CNV could strongly enhance ClREC2 expression,consequently increasing the expression of carotenoid biosynthesis genes,the number of plastoglobules within chromoplasts,and carotenoid level in mature fruit flesh.Finally,we proposed a“two-switch”genetic model by integrating two major causative loci,which can explain the formation of the four main flesh colors in different watermelon accessions.These results provide new insights into the regulation of carotenoid biosynthesis and color formation in plants.
