Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breedin...Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.展开更多
Objective:To evaluate the changes occur in the vagina, cervix and endometrium during the pre- ovulatory period in Arabian mares by using endoscope as a diagnostic tool as well as the availability of taking an endoscop...Objective:To evaluate the changes occur in the vagina, cervix and endometrium during the pre- ovulatory period in Arabian mares by using endoscope as a diagnostic tool as well as the availability of taking an endoscopic biopsy during this stage.Methods:Ten cyclic and healthy arabian mares were subjected to ultrasound scanning of their ovaries to estimate the diameter of the preovulatory follicles, collection of blood samples for estimating the hormonal levels (Estradiol-17β and progestrone) for confirming the estrous phase, endoscopic monitoring their vagina, cervix and endometrium, the availability of taking biopsical samples and histological examination of the obtained samples.Results:The obtained data revealed that, the mean diameter of the preovulatory follicles was (3.95±0.34) cm, the plasma estradiol and progestrone levels were (85.98±5.26) pg/mL and (0.73±0.23) ng/mL, respectively, endoscopic monitoring the vagina, cervix and endometrium revealed hyperemic vaginal mucous membrane, soft and opened cervical ostium with abundant estrous mucous and folding, hyperemic and edematous appearance of the endometrium, respectively and the histological features of the biopsical endometrial samples revealed that, The lamina propria-sub mucosa was packed with simple or branched tubular glands lined with simple columnar epithelium showing oval or rounded dark nuclei with different degrees of secretory activities of the glands.Conclusions: The endoscope proved to be a reliable tool for confirming the estrous phase in mares and monitoring the gross features of the vagina, the cervix and the endometrium, in addition to the availability of taking biopsical smears.展开更多
Objective:To enhance effectiveness of reproduction management in Arabian mares,factorsinfluencing the time of foaling were investigated in this study.Methods:Data were collectedat the National Haras of Tiaret in Alger...Objective:To enhance effectiveness of reproduction management in Arabian mares,factorsinfluencing the time of foaling were investigated in this study.Methods:Data were collectedat the National Haras of Tiaret in Algeria from 2003 to 2010.The foaling time of 255 Arabian purebred mares,aged from 3 to 20 years were used for this study.Results:A total of 78.07%of foalinghappens between 7 pm and 6 am.Conclusions:The influence of the month of foaling and the sexof the foal,on the time of foaling was statically significant.展开更多
Background Ovarian follicular fluid(FF)is a dynamic environment that changes with the seasons,affecting follicle development,ovulation,and oocyte quality.Cells in the follicles release tiny particles called extracellu...Background Ovarian follicular fluid(FF)is a dynamic environment that changes with the seasons,affecting follicle development,ovulation,and oocyte quality.Cells in the follicles release tiny particles called extracellular vesicles(EVs)containing vital regulatory molecules,such as microRNAs(miRNAs).These miRNAs are pivotal in facilitating commu-nication within the follicles through diverse signaling and information transfer forms.EV-coupled miRNA signaling is implicated to be associated with ovarian function,follicle and oocyte growth and response to various environmen-tal insults.Herein,we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses.Results Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the ano-vulatory(spring:non-breeding)and ovulatory(spring,summer,and fall:breeding)seasons and subsequent EV isola-tion and miRNA profiling identified significant variation in EV-miRNA cargo content.We identified 97 miRNAs with dif-ferential expression among the groups and specific clusters of miRNAs involved in the spring transition(miR-149,-200b,-206,-221,-328,and-615)and peak breeding period(including miR-143,-192,-451,-302b,-100,and let-7c).Bioinformatic analyses showed enrichments in various biological functions,e.g.,transcription factor activity,transcrip-tion and transcription regulation,nucleic acid binding,sequence-specific DNA binding,p53 signaling,and post-trans-lational modifications.Cluster analyses revealed distinct sets of significantly up-and down-regulated miRNAs associ-ated with spring anovulatory(Cluster 1)and summer ovulation–the peak breeding season(Clusters 4 and 6).Conclusions The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons,and their roles in understanding the mechanisms involved in seasonal shifts and ovulation during the breeding season warrant further investigation.展开更多
In this work,we aim to investigate the origin of the magnetic carriers in the lunar crust and the intensity of the ancient dynamo field.The magnetization and depth range of magnetic carriers are studied under a weak a...In this work,we aim to investigate the origin of the magnetic carriers in the lunar crust and the intensity of the ancient dynamo field.The magnetization and depth range of magnetic carriers are studied under a weak and a strong magnetic anomaly in Mare Tranquillitatis and in Oceanus Procellarum,respectively,where the surface ages are 3.6 and 3.3 billion years.A sophisticated three-dimensional amplitude inversion software program from a geophysical survey is used to reconstruct the distributions of magnetization in the lunar crust.Because no globally measured surface magnetic field exists for the Moon,a crustal magnetic anomaly model with a grid resolution of 0.2°is used.The depth range of the magnetic source is fixed by the boundary identified by a relative criterion,which is 20%of the recovered maximum magnetization.The central burial depths of the magnetic carriers are approximately 15 km and 25 km under Reiner Gamma and Mare Tranquillitatis,respectively.The volumes of the two magnetic sources are at scales of 104 and 105 km3,respectively.The aforementioned differences may imply a hotter crust under Reiner Gamma than Mare Tranquillitatis by 3.3 billion years.The results support the view that the magma intrusions magnetized by an ancient magnetic field could be the origin of magnetic anomalies under Reiner Gamma and Mare Tranquillitatis.Compared with previous works,the maximum magnetization of 3 A/m under Reiner Gamma supports the intensity of the field being several microteslas.展开更多
Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an u...Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an unusual gram-positive bacterium which can also be a zoonotic agent. Furthermore, the isolated strain showed a worrying multidrug-resistant profile. The accurate finding of a successful antimicrobial treatment and consequently,the pregnancy diagnosis indicate the importance to isolate, identify and define the antibiotic resistance profile of bacteria associated with endometritis.展开更多
The aim of this study was to determine the influence of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of 25 crossbred mares were studies. Two endom...The aim of this study was to determine the influence of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of 25 crossbred mares were studies. Two endometrial samples were collected from each mare,1 inthe estrus and1 inthe diestrus phase. The samples were classified according to histological changes. Collagen was typed and quantified using the picrosirius red histochemical technique, and steroid receptors were identified by immunohistochemistry. The results showed a predominance of collagen type III in all the endometrial samples. The expression of estrogen (RE2) and progesterone (RP4) receptors varied according to the estrous cycle. RE2 and RP4 expression varied in the estrus and diestrus phases;there was no influence of collagen I or II on receptor expression.展开更多
The Moscoviense basin is an atypical lunar impact basin with concentric rings of positive and negative gravity anomalies. This basin can provide insights into the inhomogeneous thermal activities across the farside of...The Moscoviense basin is an atypical lunar impact basin with concentric rings of positive and negative gravity anomalies. This basin can provide insights into the inhomogeneous thermal activities across the farside of the Moon. Based on an updated spherical harmonic thin elastic-shell loading model, we used localized admittance analyses to estimate the elastic thickness as well as other associated selenophysical parameters for the Moscoviense basin. The high precision gravity and topography data employed in our estimation were collected by the Gravity Recovery and Interior Laboratory and the Lunar Orbiter Laser Altimeter missions. Our results indicate that the crust-mantle interface is mainly compensated by the prefilling depth rather than the observed surface topography. The results constrained within two standard deviations yielded a small load ratio(~0.168), a best-fit crustal thickness of 36.2 km, and an optimized crustal density of 3159.5 kg m-3. Such large density approaches the density of olivine-rich mantle materials, implying that the excavation of the Mare Moscoviense occurred during a basin-forming impact. The inversed elastic thickness at Mare Moscoviense was around 18 km, lower than the previous results(~60 km) found over Mare basins on the lunar nearside. These results indicate that extreme thermal activity existed during the Moscoviense basin-forming period such as reheating mechanisms from a double-impact process and mare volcanism.展开更多
The lunar mare potassium(K)-,rare-earth elements(REEs)-and phosphorous(P)-rich(KREEP-rich) region is a unique late-stage product of magma crystallization,in which ilmenite and incompatible elements have high grades,th...The lunar mare potassium(K)-,rare-earth elements(REEs)-and phosphorous(P)-rich(KREEP-rich) region is a unique late-stage product of magma crystallization,in which ilmenite and incompatible elements have high grades,thus forming a giant natural reservoir.The extraction and purification of the high-value metal resources in the KREEP-rich region not only meet the construction needs of the lunar base but also solve the problem of resource scarcity on Earth.In this study,photovoltaic elemental silicon(Si) was used as a collector to extract ilmenite resources,REEs,and nuclear energy elements from basalt in the lunar mare KREEP-rich region at 1873 K.Based on experimentation,the metals titanium(Ti)and iron(Fe) in the lunar mare ilmenite are found to be enriched and solidified in the form of Si-based alloys.The contents of valuable incompatible elements in the KREEP-rich area are also found to be enriched and contained in the incompatible trace elements(ITEs) phase of the alloy.Among them,REEs(e.g.,cerium(Ce) and thulium(Tm)) and nuclear elements(e.g.,thorium(Th) and uranium(U)) are found to account for 82.61 wt% of the ITEs phase.This process provides a simple and feasible scheme for the insitu resource utilization(ISRU) of the lunar surface and is suitable for the extraction and enrichment of lunar metal resources.展开更多
Two new lunar mare soil simulants,NAO-2 and NAO-3,have been created in National Astronomical Observatories(NAO),Chinese Academy of Sciences.These two simulants were produced from low-titanium basalt and high-titanium ...Two new lunar mare soil simulants,NAO-2 and NAO-3,have been created in National Astronomical Observatories(NAO),Chinese Academy of Sciences.These two simulants were produced from low-titanium basalt and high-titanium basalt respectively.The chemical composition,mineralogy, particle size distribution,density,angle of internal friction,and cohesion of both simulants have been analyzed,indicating that some characteristics of NAO-2 and NAO-3 are similar to those of Apollo 14 and Apollo 11 landing site soils.NAO-2 and NAO-3 will be of great benefit to the scientific and engineering research on lunar soil.展开更多
The Dutch herring trade contributed greatly to Dutch commercial prosperity. The most profitable Dutch fishing grounds, however, were off the coasts of England and Scotland. For centuries, the English allowed the Dutch...The Dutch herring trade contributed greatly to Dutch commercial prosperity. The most profitable Dutch fishing grounds, however, were off the coasts of England and Scotland. For centuries, the English allowed the Dutch to fish these waters. During the reign of James I, however, the changing English perspective of the Dutch led the English Crown to question the right of the Dutch to fish in English waters. In the fishing trade, centuries of cooperation gave way to an emerging commercial rivalry.展开更多
The authentication of milk requires the use of sophisticated and expensive analytical techniques. There is a huge need for reliable and cheap analytical technologies for use as fast and effective screening methods. Th...The authentication of milk requires the use of sophisticated and expensive analytical techniques. There is a huge need for reliable and cheap analytical technologies for use as fast and effective screening methods. This paper proposes the use of myristic acid in the authentication of cow, mare, camel and goat milk, using near infrared spectrometry. Comparison has been made with traditional gas chromatography methods, so that both methods can be used in the authentication of different types of dairy products.展开更多
Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will...Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will be a racehorse winner!” In January 1945, Jacqueline du Pré, the remarkable, world-famous cellist was born. In the 1920's and 1930's, animal experimentation and clinical studies had shown that pituitary glycoproteins stimulated the ovary (follicle-stimulating hormone, FSH) and the corpus luteum (luteal-stimulating hormone, LH) which prepared the human womb for embedding a fertilized ovum and that pregnant mare’s blood and urine contained the glycoprotein, PMSG whose origin was placental cells, but surprisingly in humans had the actions of both FSH and LH. However, the PMSG serum alone did not bring about pregnancy. The doctor did not know that without subsequent injection of another factor in correct sequence and timing, PMSG was pointless. In 1947, a placental glycoprotein, found in the 1920's in urine of pregnant women (human chorionic gonadotropin, hCG), when injected in mice subsequent to PMSG, achieved ovulation but not pregnancy. Human application of those findings was extremely risky due to impurities (up to 95%). The Federal Drug Administration (FDA), established in 1938, requested easily bye-passed marketing safety. Companies offered material “sufficiently” purified;professional bodies negated clinical use, tempting to a few. Evidence also suggests that, to sustain pregnancy the doctor also prescribed the new “eostrogen”, diethyl stilbestrol (DES) of negative fame. In 1947, the Nuremberg Code of ethics demanded human experiments by qualified personnel and trials preceded by adequate animal studies. It is not the case here. From five, du Pré had a most exceptional musical memory, almost obsessive musicality and a very difficult school-time socially. Later history: adult masculine build, awkward gait, tendency to recurrent depressions from mid-adolescence, unbalanced thyroidal metabolism, symptoms of numbness in late teens, long breaks for rest from age 25, MS diagnosis at 28 when unable to play, death aged 42. Yet at sixteen and after, she astounded all with technique, passion and unique musical interpretation. Her husband, an outstanding musician: “She had a capacity to imagine sound such as I never met in any other musician”. A close musician colleague: “... it was done before she was born”;perhaps much closer to the truth than realization, for her history it may suggest a fetal neurodevelopment abused in the womb.展开更多
The Book of Odes formed the Chinese sensibility and constituted basic teaching material for two millennia.It also has been one of the main entry doors through which Western sinologists approached the world of ancient ...The Book of Odes formed the Chinese sensibility and constituted basic teaching material for two millennia.It also has been one of the main entry doors through which Western sinologists approached the world of ancient China.From the 17th century onwards,Chinese and Western readings have crisscrossed,giving new depth to the hermeneutics of the Odes.This contribution focuses on these hermeneutical encounters.Its first part traces the lines of interpretation suggested by their authorized exegete,Confucius,and the way this tradition was challenged by Zhu Xi.Some Western commentators will preferentially adhere to Confucius and its epigones,others will mobilize Zhu Xi's theoretical openings for their own purpose.The second part analyzes how Marcel Granet has read the Odes according to their ritual context and the role they play within it.The third part broadens Granet’s approach,showing how the Odes can be approached as an ethnographic document,particularly in terms of religious ethnography,a task to which both Chinese and foreign authors have contributed.The fourth part looks at some of the readings that found an aesthetic standard in the Odes,in classical China and,as in echo,in the early history of their transmission in other languages.The fifth part extends this analysis,focusing on the updating of the structural rhetoric specific to the Odes.Finally,the last section returns to Confucius,not as he appears in the Analects,but as the author-function associated with the manuscript Confucius Speaks of the Odes(Kongzi shilun),a text that revives the question of the intention to be attached to the reading of the Odes and that constitutes a meeting point of present-day Chinese and Western scholarly concerns.展开更多
The Moon’s KREEP component—rich in potassium(K),rare earth elements(REE),and phosphorus(P)—is considered a critical heat source sustaining prolonged volcanic activity.However,Chang’e mission samples reveal a lack ...The Moon’s KREEP component—rich in potassium(K),rare earth elements(REE),and phosphorus(P)—is considered a critical heat source sustaining prolonged volcanic activity.However,Chang’e mission samples reveal a lack of KREEP signatures in the sources of mare basalt erupted∼2.8 and 2.0 billion years ago,raising questions about its contribution to late-stage volcanism.Here,we present geochemical and geochronological analyses of the lunar basaltic meteorite NWA 16286,which crystallized 2201±13 million years ago,representing the youngest known lunar meteorite.Its mantle source shows a high^(238)U/^(204)Pb ratio(∼2000)and a lowεNd(t)value(–4.3),indicating strong KREEP enrichment and extending the duration of KREEP-related magmatism by∼800 million years.Compiled isotopic data from lunar basalts reveal three volcanic episodes,each characterized by progressively increasing 238U/204Pb and decreasingεNd,suggesting episodic mantle convection.We propose that episodic sluggish mantle overturn or plume-driven upwelling triggered initial decompression melting,followed by increasing incorporation of KREEP components into basalt sources during each episode of younger mare volcanism.展开更多
The evolution of the Moon is driven by both endogenic(e.g.,magmatism)and exogenic processes(e.g.,impact).The lunar surface can be divided into 3 first-order tectonic units based on differences in geochemistry,crustal ...The evolution of the Moon is driven by both endogenic(e.g.,magmatism)and exogenic processes(e.g.,impact).The lunar surface can be divided into 3 first-order tectonic units based on differences in geochemistry,crustal thickness,topography,and distribution of structures.However,the interpreted boundaries demarcated by different features varied widely.As research progresses,the understanding of the lunar structure is continuously improving,and new types of structures are constantly being discovered.The previous structural classification system needs to be updated.According to the major geological events and dynamic evolution,the evolutionary history of the Moon can be divided into 3 stages and 1 pivotal event.The first stage(4.52 to 4.3 Ga)is the evolution of the magmatic ocean,and it was dominated by endogenic processes.The formation of South Pole-Aitken(SPA)basin(~4.3 Ga)is the pivotal event of crustal evolution and indicates that the lunar crust had cooled and consolidated to sufficient rigidity to preserve structures.Both endogenic and exogenic processes were active in the second stage(4.3 to 3.0 Ga),which results in numerous impact basins,mare basalt flooding,and different types of associated structures.The last stage(3.0 Ga to the present)is late neotectonic activity,which has weak modification of the tectonic framework.Response of tectonic framework and structure to lunar major geological events had been organized and discussed in this paper,and key scientific issues are summarized.This study can provide reference and support for implementation of subsequent lunar exploration projects.展开更多
基金Supported by Faculty of Veterinary Medicine.University of Naples"Federico II".Naples Italy
文摘Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.
文摘Objective:To evaluate the changes occur in the vagina, cervix and endometrium during the pre- ovulatory period in Arabian mares by using endoscope as a diagnostic tool as well as the availability of taking an endoscopic biopsy during this stage.Methods:Ten cyclic and healthy arabian mares were subjected to ultrasound scanning of their ovaries to estimate the diameter of the preovulatory follicles, collection of blood samples for estimating the hormonal levels (Estradiol-17β and progestrone) for confirming the estrous phase, endoscopic monitoring their vagina, cervix and endometrium, the availability of taking biopsical samples and histological examination of the obtained samples.Results:The obtained data revealed that, the mean diameter of the preovulatory follicles was (3.95±0.34) cm, the plasma estradiol and progestrone levels were (85.98±5.26) pg/mL and (0.73±0.23) ng/mL, respectively, endoscopic monitoring the vagina, cervix and endometrium revealed hyperemic vaginal mucous membrane, soft and opened cervical ostium with abundant estrous mucous and folding, hyperemic and edematous appearance of the endometrium, respectively and the histological features of the biopsical endometrial samples revealed that, The lamina propria-sub mucosa was packed with simple or branched tubular glands lined with simple columnar epithelium showing oval or rounded dark nuclei with different degrees of secretory activities of the glands.Conclusions: The endoscope proved to be a reliable tool for confirming the estrous phase in mares and monitoring the gross features of the vagina, the cervix and the endometrium, in addition to the availability of taking biopsical smears.
基金Supported by project CNEPRU,Institute of Veterinary Sciences,University Ibn-Khaldoun(TIARET)Algeria(Grant No.F001 2010/0016)
文摘Objective:To enhance effectiveness of reproduction management in Arabian mares,factorsinfluencing the time of foaling were investigated in this study.Methods:Data were collectedat the National Haras of Tiaret in Algeria from 2003 to 2010.The foaling time of 255 Arabian purebred mares,aged from 3 to 20 years were used for this study.Results:A total of 78.07%of foalinghappens between 7 pm and 6 am.Conclusions:The influence of the month of foaling and the sexof the foal,on the time of foaling was statically significant.
基金Southern Illinois University,Carbondale,ILMinistry of Higher Education&Scientific Research,Baghdad,Iraq+2 种基金NIFA-USDA Hatch project accession#1016077(Multistate#W4171)USDAARS project 6066-31000-015-00DNIH MS-IDeA network of Biomedical Research Excellence award 5P20GMI03476-19.GMI received a PhD scholarship from the Ministry of Higher Education&Scientific Research,Baghdad,Iraq.
文摘Background Ovarian follicular fluid(FF)is a dynamic environment that changes with the seasons,affecting follicle development,ovulation,and oocyte quality.Cells in the follicles release tiny particles called extracellular vesicles(EVs)containing vital regulatory molecules,such as microRNAs(miRNAs).These miRNAs are pivotal in facilitating commu-nication within the follicles through diverse signaling and information transfer forms.EV-coupled miRNA signaling is implicated to be associated with ovarian function,follicle and oocyte growth and response to various environmen-tal insults.Herein,we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses.Results Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the ano-vulatory(spring:non-breeding)and ovulatory(spring,summer,and fall:breeding)seasons and subsequent EV isola-tion and miRNA profiling identified significant variation in EV-miRNA cargo content.We identified 97 miRNAs with dif-ferential expression among the groups and specific clusters of miRNAs involved in the spring transition(miR-149,-200b,-206,-221,-328,and-615)and peak breeding period(including miR-143,-192,-451,-302b,-100,and let-7c).Bioinformatic analyses showed enrichments in various biological functions,e.g.,transcription factor activity,transcrip-tion and transcription regulation,nucleic acid binding,sequence-specific DNA binding,p53 signaling,and post-trans-lational modifications.Cluster analyses revealed distinct sets of significantly up-and down-regulated miRNAs associ-ated with spring anovulatory(Cluster 1)and summer ovulation–the peak breeding season(Clusters 4 and 6).Conclusions The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons,and their roles in understanding the mechanisms involved in seasonal shifts and ovulation during the breeding season warrant further investigation.
基金supported by the National Key R&D Program of China (Grant No. 2021YFA0715101)supported by the Chinese 111 Project (Contract No. B20011)+1 种基金the Fundamental Research Funds for the Central Universitiessupported by the Innovation Experimental Class Program
文摘In this work,we aim to investigate the origin of the magnetic carriers in the lunar crust and the intensity of the ancient dynamo field.The magnetization and depth range of magnetic carriers are studied under a weak and a strong magnetic anomaly in Mare Tranquillitatis and in Oceanus Procellarum,respectively,where the surface ages are 3.6 and 3.3 billion years.A sophisticated three-dimensional amplitude inversion software program from a geophysical survey is used to reconstruct the distributions of magnetization in the lunar crust.Because no globally measured surface magnetic field exists for the Moon,a crustal magnetic anomaly model with a grid resolution of 0.2°is used.The depth range of the magnetic source is fixed by the boundary identified by a relative criterion,which is 20%of the recovered maximum magnetization.The central burial depths of the magnetic carriers are approximately 15 km and 25 km under Reiner Gamma and Mare Tranquillitatis,respectively.The volumes of the two magnetic sources are at scales of 104 and 105 km3,respectively.The aforementioned differences may imply a hotter crust under Reiner Gamma than Mare Tranquillitatis by 3.3 billion years.The results support the view that the magma intrusions magnetized by an ancient magnetic field could be the origin of magnetic anomalies under Reiner Gamma and Mare Tranquillitatis.Compared with previous works,the maximum magnetization of 3 A/m under Reiner Gamma supports the intensity of the field being several microteslas.
文摘Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an unusual gram-positive bacterium which can also be a zoonotic agent. Furthermore, the isolated strain showed a worrying multidrug-resistant profile. The accurate finding of a successful antimicrobial treatment and consequently,the pregnancy diagnosis indicate the importance to isolate, identify and define the antibiotic resistance profile of bacteria associated with endometritis.
文摘The aim of this study was to determine the influence of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of 25 crossbred mares were studies. Two endometrial samples were collected from each mare,1 inthe estrus and1 inthe diestrus phase. The samples were classified according to histological changes. Collagen was typed and quantified using the picrosirius red histochemical technique, and steroid receptors were identified by immunohistochemistry. The results showed a predominance of collagen type III in all the endometrial samples. The expression of estrogen (RE2) and progesterone (RP4) receptors varied according to the estrous cycle. RE2 and RP4 expression varied in the estrus and diestrus phases;there was no influence of collagen I or II on receptor expression.
基金supported by the National Natural Science Foundation of China (41864001 and U1831132)Guizhou Science and Technology Plan Project (Guizhou Science and Technology platform talents [2018]5769)+5 种基金Open Fund of State Laboratory of Information Engineering in Surveying, Mapping and Remote Sensing, Wuhan University (Grant No. 17P03)Open Fund of Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education (Guangxi Teachers Education University)Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation (Guangxi Teachers Education University, No. 2015K03)Open Fund of Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing (KF201813)Open Fund of Lunar and Planetary Science Laboratory, Macao University of Science and Technology (FDCT 119/2017/A3)Hubei Province Foundation innovation group project (2018CFA087)
文摘The Moscoviense basin is an atypical lunar impact basin with concentric rings of positive and negative gravity anomalies. This basin can provide insights into the inhomogeneous thermal activities across the farside of the Moon. Based on an updated spherical harmonic thin elastic-shell loading model, we used localized admittance analyses to estimate the elastic thickness as well as other associated selenophysical parameters for the Moscoviense basin. The high precision gravity and topography data employed in our estimation were collected by the Gravity Recovery and Interior Laboratory and the Lunar Orbiter Laser Altimeter missions. Our results indicate that the crust-mantle interface is mainly compensated by the prefilling depth rather than the observed surface topography. The results constrained within two standard deviations yielded a small load ratio(~0.168), a best-fit crustal thickness of 36.2 km, and an optimized crustal density of 3159.5 kg m-3. Such large density approaches the density of olivine-rich mantle materials, implying that the excavation of the Mare Moscoviense occurred during a basin-forming impact. The inversed elastic thickness at Mare Moscoviense was around 18 km, lower than the previous results(~60 km) found over Mare basins on the lunar nearside. These results indicate that extreme thermal activity existed during the Moscoviense basin-forming period such as reheating mechanisms from a double-impact process and mare volcanism.
基金supported by the Yunnan Outstanding Youth Science Foundation (202101AV070007)the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province (2018HB009)Strategic Priority Research Program of the Chinese Academy of Sciences (XDB 41000000)。
文摘The lunar mare potassium(K)-,rare-earth elements(REEs)-and phosphorous(P)-rich(KREEP-rich) region is a unique late-stage product of magma crystallization,in which ilmenite and incompatible elements have high grades,thus forming a giant natural reservoir.The extraction and purification of the high-value metal resources in the KREEP-rich region not only meet the construction needs of the lunar base but also solve the problem of resource scarcity on Earth.In this study,photovoltaic elemental silicon(Si) was used as a collector to extract ilmenite resources,REEs,and nuclear energy elements from basalt in the lunar mare KREEP-rich region at 1873 K.Based on experimentation,the metals titanium(Ti)and iron(Fe) in the lunar mare ilmenite are found to be enriched and solidified in the form of Si-based alloys.The contents of valuable incompatible elements in the KREEP-rich area are also found to be enriched and contained in the incompatible trace elements(ITEs) phase of the alloy.Among them,REEs(e.g.,cerium(Ce) and thulium(Tm)) and nuclear elements(e.g.,thorium(Th) and uranium(U)) are found to account for 82.61 wt% of the ITEs phase.This process provides a simple and feasible scheme for the insitu resource utilization(ISRU) of the lunar surface and is suitable for the extraction and enrichment of lunar metal resources.
基金supported by The National High Technology Research and Development Program of China(No.2008AA 12A213)The National Key Scientific and Technologic Project
文摘Two new lunar mare soil simulants,NAO-2 and NAO-3,have been created in National Astronomical Observatories(NAO),Chinese Academy of Sciences.These two simulants were produced from low-titanium basalt and high-titanium basalt respectively.The chemical composition,mineralogy, particle size distribution,density,angle of internal friction,and cohesion of both simulants have been analyzed,indicating that some characteristics of NAO-2 and NAO-3 are similar to those of Apollo 14 and Apollo 11 landing site soils.NAO-2 and NAO-3 will be of great benefit to the scientific and engineering research on lunar soil.
文摘The Dutch herring trade contributed greatly to Dutch commercial prosperity. The most profitable Dutch fishing grounds, however, were off the coasts of England and Scotland. For centuries, the English allowed the Dutch to fish these waters. During the reign of James I, however, the changing English perspective of the Dutch led the English Crown to question the right of the Dutch to fish in English waters. In the fishing trade, centuries of cooperation gave way to an emerging commercial rivalry.
文摘The authentication of milk requires the use of sophisticated and expensive analytical techniques. There is a huge need for reliable and cheap analytical technologies for use as fast and effective screening methods. This paper proposes the use of myristic acid in the authentication of cow, mare, camel and goat milk, using near infrared spectrometry. Comparison has been made with traditional gas chromatography methods, so that both methods can be used in the authentication of different types of dairy products.
文摘Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will be a racehorse winner!” In January 1945, Jacqueline du Pré, the remarkable, world-famous cellist was born. In the 1920's and 1930's, animal experimentation and clinical studies had shown that pituitary glycoproteins stimulated the ovary (follicle-stimulating hormone, FSH) and the corpus luteum (luteal-stimulating hormone, LH) which prepared the human womb for embedding a fertilized ovum and that pregnant mare’s blood and urine contained the glycoprotein, PMSG whose origin was placental cells, but surprisingly in humans had the actions of both FSH and LH. However, the PMSG serum alone did not bring about pregnancy. The doctor did not know that without subsequent injection of another factor in correct sequence and timing, PMSG was pointless. In 1947, a placental glycoprotein, found in the 1920's in urine of pregnant women (human chorionic gonadotropin, hCG), when injected in mice subsequent to PMSG, achieved ovulation but not pregnancy. Human application of those findings was extremely risky due to impurities (up to 95%). The Federal Drug Administration (FDA), established in 1938, requested easily bye-passed marketing safety. Companies offered material “sufficiently” purified;professional bodies negated clinical use, tempting to a few. Evidence also suggests that, to sustain pregnancy the doctor also prescribed the new “eostrogen”, diethyl stilbestrol (DES) of negative fame. In 1947, the Nuremberg Code of ethics demanded human experiments by qualified personnel and trials preceded by adequate animal studies. It is not the case here. From five, du Pré had a most exceptional musical memory, almost obsessive musicality and a very difficult school-time socially. Later history: adult masculine build, awkward gait, tendency to recurrent depressions from mid-adolescence, unbalanced thyroidal metabolism, symptoms of numbness in late teens, long breaks for rest from age 25, MS diagnosis at 28 when unable to play, death aged 42. Yet at sixteen and after, she astounded all with technique, passion and unique musical interpretation. Her husband, an outstanding musician: “She had a capacity to imagine sound such as I never met in any other musician”. A close musician colleague: “... it was done before she was born”;perhaps much closer to the truth than realization, for her history it may suggest a fetal neurodevelopment abused in the womb.
文摘The Book of Odes formed the Chinese sensibility and constituted basic teaching material for two millennia.It also has been one of the main entry doors through which Western sinologists approached the world of ancient China.From the 17th century onwards,Chinese and Western readings have crisscrossed,giving new depth to the hermeneutics of the Odes.This contribution focuses on these hermeneutical encounters.Its first part traces the lines of interpretation suggested by their authorized exegete,Confucius,and the way this tradition was challenged by Zhu Xi.Some Western commentators will preferentially adhere to Confucius and its epigones,others will mobilize Zhu Xi's theoretical openings for their own purpose.The second part analyzes how Marcel Granet has read the Odes according to their ritual context and the role they play within it.The third part broadens Granet’s approach,showing how the Odes can be approached as an ethnographic document,particularly in terms of religious ethnography,a task to which both Chinese and foreign authors have contributed.The fourth part looks at some of the readings that found an aesthetic standard in the Odes,in classical China and,as in echo,in the early history of their transmission in other languages.The fifth part extends this analysis,focusing on the updating of the structural rhetoric specific to the Odes.Finally,the last section returns to Confucius,not as he appears in the Analects,but as the author-function associated with the manuscript Confucius Speaks of the Odes(Kongzi shilun),a text that revives the question of the intention to be attached to the reading of the Odes and that constitutes a meeting point of present-day Chinese and Western scholarly concerns.
基金supported by the National Natural Science Foundation of China(42225301,42241105,and 42403008)the Strategy Priority Research Program(Category B)of the Chinese Academy of Sciences(XDB0710000)the Key Research Program of the Institute of Geology and Geophysics,Chinese Academy of Sciences(IGGCAS-202401).
文摘The Moon’s KREEP component—rich in potassium(K),rare earth elements(REE),and phosphorus(P)—is considered a critical heat source sustaining prolonged volcanic activity.However,Chang’e mission samples reveal a lack of KREEP signatures in the sources of mare basalt erupted∼2.8 and 2.0 billion years ago,raising questions about its contribution to late-stage volcanism.Here,we present geochemical and geochronological analyses of the lunar basaltic meteorite NWA 16286,which crystallized 2201±13 million years ago,representing the youngest known lunar meteorite.Its mantle source shows a high^(238)U/^(204)Pb ratio(∼2000)and a lowεNd(t)value(–4.3),indicating strong KREEP enrichment and extending the duration of KREEP-related magmatism by∼800 million years.Compiled isotopic data from lunar basalts reveal three volcanic episodes,each characterized by progressively increasing 238U/204Pb and decreasingεNd,suggesting episodic mantle convection.We propose that episodic sluggish mantle overturn or plume-driven upwelling triggered initial decompression melting,followed by increasing incorporation of KREEP components into basalt sources during each episode of younger mare volcanism.
基金supported by the National Key Research and Development Program of China(Grant No.2022YFF0503100)the National Natural Science Foundation of China(Grant No.42202264)+2 种基金the National Natural Science Foundation of China-Academic Divisions of Chinese Academy of Sciences Frontier Interdisciplinary Research Strategic Research Joint Funding Project(Grant No.L2224032,XK2022DXC004)the Key Research Program of the Chinese Academy of Sciences,(Grant No.KGFZD-145-2023-15)Guizhou Provincial Science and Technology Projects(Grant No.[QKHJC-ZK(2023)-478]).
文摘The evolution of the Moon is driven by both endogenic(e.g.,magmatism)and exogenic processes(e.g.,impact).The lunar surface can be divided into 3 first-order tectonic units based on differences in geochemistry,crustal thickness,topography,and distribution of structures.However,the interpreted boundaries demarcated by different features varied widely.As research progresses,the understanding of the lunar structure is continuously improving,and new types of structures are constantly being discovered.The previous structural classification system needs to be updated.According to the major geological events and dynamic evolution,the evolutionary history of the Moon can be divided into 3 stages and 1 pivotal event.The first stage(4.52 to 4.3 Ga)is the evolution of the magmatic ocean,and it was dominated by endogenic processes.The formation of South Pole-Aitken(SPA)basin(~4.3 Ga)is the pivotal event of crustal evolution and indicates that the lunar crust had cooled and consolidated to sufficient rigidity to preserve structures.Both endogenic and exogenic processes were active in the second stage(4.3 to 3.0 Ga),which results in numerous impact basins,mare basalt flooding,and different types of associated structures.The last stage(3.0 Ga to the present)is late neotectonic activity,which has weak modification of the tectonic framework.Response of tectonic framework and structure to lunar major geological events had been organized and discussed in this paper,and key scientific issues are summarized.This study can provide reference and support for implementation of subsequent lunar exploration projects.
