[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 3...[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera.[ Conclusion] It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.展开更多
Objective:To evaluate the antibacterial activity of the methanolic extract of mango(Mangifera indica L) seed kernel.Methods:Chokanan mango seed kernel and seed kernels from assorted mango varieties were collected,clea...Objective:To evaluate the antibacterial activity of the methanolic extract of mango(Mangifera indica L) seed kernel.Methods:Chokanan mango seed kernel and seed kernels from assorted mango varieties were collected,cleaned,dried and powered.Crude methanolic extracts of mango seed kernel were analyzed for the phytochemical constituents.The free radical scavenging activity was determined by 2,2-Diphenyl-1-picrylhydrazyl(DPPH) assay.Antibacterial activity was evaluated by disc diffusion assay with three medically important bacterial pathogens such as methicillin resistant Staphylococcus aureus(S.aureus)(MRSA),Escherichia coli(E.coli) and Vibrio vulnificus(V.vulnificus).Results:Qualitative phytochemical analysis indicated the presence of important phytochemical compounds such as glycosides,saponins,flavanoids, tannins and alkaloids.There was no significant difference in the phytochemical content between the single and assorted mango seed kernels.However,the free radical scavenging study indicated that the assorted mango kernels showed slighdy higher activity than the single species(P【0.05). The crude methanolic extract of mango seed kernel at a concentration of 100 mg/mL is found to have potential antimicrobial activity against MRSA and E.coli compared to V.vulnificus. Study on the antibacterial activity also indicated that there was no significant difference in the antibacterial activity of the single and assorted mango seed kernel extracts.Conclusions:The present study conclusively demonstrates the free radical scavenging activity and antibacterial activities of mango seed kernel.In addition,the results also indicated that there is no significant difference in the phytochemical content and biological activity of mango kernels from single and assorted mango varieties.展开更多
Objective:To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica(M.indica) and Mucuna urens(M.urens) seeds extracts against some key enzymes(-amylase,-glucosidasc and aldose ...Objective:To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica(M.indica) and Mucuna urens(M.urens) seeds extracts against some key enzymes(-amylase,-glucosidasc and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro.Methods:Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection.The inhibitory activities of the seeds extracts against-amylase and-glucosidase were estimated using soluble starch and p-nitrophenylglucopyranosidc as their respective substrates.Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme;inhibition of Fe^(2+)-induced lipid peroxidation by extracts was tested in rat pancreas homogenate.Results:The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance,including gallic acid,chlorogenic acid,caffeic acid,ellagic acid,catechin,rutin,quercitrin.quercetin and kaempferol.Extracts of both seeds effectively inhibited-amylase,-glucosidase and aldose reductase activities in a dose-dependent manner,having inhibitory preference for these enzymes in the order of aldose reductase>-glucosidase>-amylase.With lower half-maximal inhibitory concentrations(IC50)against-amylase,-glucosidase,and aldose reductase,M.indica had stronger inhibitory potency against these enzymes than M.urens.Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern,with M.indica being more potent than M.urens.Conclusions:The results obtained provide support for a possible use of M.indica and M.urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2diabetes.展开更多
Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase（LOX）...Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase（LOX） inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively.Methanolic extract（MEMI）,successive water extract （SWMI） and ethyl acetate fraction（EMEMI）,n-butanol fraction（BMEMI） and water soluble fraction （WMEMI） of methanolic extract were evaluated along with respective reference standards. Results:In in ritro DPPH radical scavenging activity,the MEMI,EMEMI and BMEMI have offered significant antioxidant activity with IC<sub>50</sub> values of 13.37.3.55 and 14.19μig/mL respectively.Gallic acid,a reference standard showed significant antioxidant activity with IC? value of 1.88 and found to be more potent compared to all the extracts and fractions.In m vitro LOX inhibition assay,the MEMI,EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC<sub>50</sub> values of 96.71.63.21 and 107.44μg/mL respectively.While,reference drug Indometlhacin also offered significant inhibtion against LOX enzyme activity with IC<sub>50</sub> of 57.75.Furthermore,MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity.Conclusions:These findings suggest that the MEMI and F.MEMT possess potent anti-oxidani and anti-inflammatory activities in in vitro conditions.展开更多
Objective: To investigate the presence of different phytoconstituents in Mangifera indica(M. indica) peel and evaluate its cytotoxicity to Artemia salina and hypoglycemic potential in Swiss albino mice.Methods: The me...Objective: To investigate the presence of different phytoconstituents in Mangifera indica(M. indica) peel and evaluate its cytotoxicity to Artemia salina and hypoglycemic potential in Swiss albino mice.Methods: The methanolic extract of M. indica peel was used to determine the presence of phytoconstituents. Brine shrimp lethality bioassay method was followed to determine the cytotoxic potential of plant extract. In the case of hypoglycemic activity, oral administration of extract at 200 and 400 mg/kg and standard glibenclamide at 10 mg/kg was done, followed by determining the percentage of reduction of plasma glucose from the initial level.Results: The methanolic extract of M. indica peel showed the presence of flavonoid,saponin, steroid, tannins, terpenoids, glycosides and alkaloids. In brine shrimp lethality bioassay, the LC_(50) of the extract and standard vincristine sulfate was found to be 2.04 and0.41 mg/mL, respectively. After 90 and 150 min, the methanolic extract at 200 and 400 mg/kg showed prominent plasma glucose reduction of 13.95%, 22.48% and 14.16%,26.18% respectively compared to standard glibenclamide showing 14.90% and 20.67% plasma glucose reduction.Conclusions: This current research affirms prominent cytotoxic and moderate hypoglycemic potential of M. indica peel. Further bioactivity guided isolation of phytoconstituents and investigation on higher animals can lead to development of new drug molecules.展开更多
Objective:To evaluate the total phenolic contents,antioxidant and antiglycation activities of leaves,barks,roots and kernels from two cultivars of Mangifera indica(Anacardiaceae).Methods:Total phenolic contents were d...Objective:To evaluate the total phenolic contents,antioxidant and antiglycation activities of leaves,barks,roots and kernels from two cultivars of Mangifera indica(Anacardiaceae).Methods:Total phenolic contents were determined by using Folin-Ciocalteu's method.The antioxidant activities were assessed by three different protocols including DPPH,oxygen radical absorbance capacity and iron(Ⅱ)chelation assays.In addition,in vitro bovine serum albumin/D-ribose assay was chosen to evaluate the antiglycation properties of the extracts.Results:All the investigated extracts were found to contain high level of total phenols as well as potent antioxidant activities.Kernel extracts showed the highest total phenol contents and DPPH radical scavenging activities whereas higher oxygen radical absorbance capacity values were observed for leave,root and bark extracts.Besides,extracts from leaves,roots and barks from both cultivars exhibited potent inhibitory effects against the formation of advanced glycation end products,with IC_(50)values lower than the standard positive control aminoguanidine.Conclusions:The potent antiglycation and antioxidative activities of these two Mangifera indica cultivars suggest a possible role in targeting aging,diabetic complications and oxidative stress related diseases.展开更多
Objective:To determine the lead bioactive compound in kernel extract of Mangifera pajang and its anti-cancer activity against human breast cancer cell lines with positive estrogen receptor(MCF-7).Methods:The methanoli...Objective:To determine the lead bioactive compound in kernel extract of Mangifera pajang and its anti-cancer activity against human breast cancer cell lines with positive estrogen receptor(MCF-7).Methods:The methanolic extract of dried powder kernel of Mangifera pajang was exposed to column chromatography for isolation.The structural elucidation of the isolated compound was characterized using infrared,nuclear magnetic resonance,mass spectrometry.Furthermore,cytotoxicity,morphological changes,flow cytometry and cell cycle arrest analyses were performed to examine the mechanism of anti-proliferation and apoptosis induced by methyl gallate against MCF-7.Results:One compound was isolated from the methanolic extract of Mangifera pajang kernel and identified as methyl gallate.The flow cytometric results demonstrated induction of apoptosis in MCF-7 cells by three concentrations of methyl gallate.The cell cycle arrest showed a significant(P<0.05)decrease in cell progression at G2/M phase of MCF-7 after treatment with 100μM of methyl gallate.The cell percentage of early and late apoptosis was significant at 10 and 100μM of methyl gallate.Also,methyl gallate treatment induced up-regulation of reactive oxygen species levels in MCF-7 cells with a reduction in superoxide dismutase levels.Conclusions:These findings indicate that isolated methyl gallate from Mangifera pajang kernel extracts induces growth inhibition and apoptosis in MCF-7 cells via up-regulating oxidative stress pathway.展开更多
Mango(Mangifera indica L.)is an important fruit crop in tropical and subtropical countries associated with many agronomic and horticultural problems,such as susceptibility to pathogens,including powdery mildew and ant...Mango(Mangifera indica L.)is an important fruit crop in tropical and subtropical countries associated with many agronomic and horticultural problems,such as susceptibility to pathogens,including powdery mildew and anthracnose,poor yield and quality,and short shelf life.Conventional breeding techniques exhibit significant limitations in improving mango quality due to the characteristics of long ripening,self-incompatibility,and high genetic heterozygosity.In recent years,much emphasis has been placed on identification of key genes controlling a certain trait through genomic association analysis and directly breeding new varieties through transgene or genotype selection of offspring.This paper reviews the latest research progress on the genome and transcriptome sequencing of mango fruit.The rapid development of genome sequencing and bioinformatics provides effective strategies for identifying,labeling,cloning,and manipulating many genes related to economically important traits.Preliminary verification of the functions of mango genes has been conducted,including genes related to flowering regulation,fruit development,and polyphenol biosynthesis.Importantly,modern biotechnology can refine existing mango varieties to meet the market demand with high economic benefits.展开更多
Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this stud...Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this study is to evaluate the acute general toxicity, antioxidant potential and antibacterial activitiy of leave’s aqueous extracts from Lannea microcarpa Engl. and K. Krause and Mangifera indica L. The use of these plants in traditional medicine motivated our choice to lead scientific studies. Methods: The aqueous decoction of the leaves is the form of use recommended by traditional healers. We used the same type of extracts for studies. Acute toxicity was studied in NMRI strain mice, with the dose of 2000 mg/kg body weight, for each plant species. The antioxidant activity is evaluated by the method of reduction of radical DPPH. The phytochemical compounds were detected with specific reagent: Alcaloids with Dragendorff’s reagent, Flavonoids with ammonia (NH<sub>4</sub>OH), Polyphenols and tannins are revelated by ferric chloride (FeCl<sub>3</sub>). Saponosides were revelated by their foaming power property. Bacterial inhibiting activity is tested by measuring the diameters’ inhibition of extracts on reference strains. Results: The aqueous extracts were not toxic at the maximum dose of 2000 mg/kg body weight, for each plant species. The extracts showed an antioxidant activity with an IC50, 20 μg/ml for Lannea microcarpa and 18 μg/ml for Mangifera indica. The extracts showed no bacterial activity on three strains of bacteria tested: Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The phytochemicals we have identified are for L. microcarpa: tannins and phenolics compounds, triterpenes, saponosides. We identified in M. indica’s extracts: tannins and phenolics compounds, triterpenes, saponosides and flavonoids.展开更多
Mangifera indica L. is a species of mango in the Anacardiaceae family. It is found in the wild in tropical regions and cultivated varieties have been introduced to other warm regions of the world. This present study a...Mangifera indica L. is a species of mango in the Anacardiaceae family. It is found in the wild in tropical regions and cultivated varieties have been introduced to other warm regions of the world. This present study aimed to investigate the in vitro antimicrobial activities of methanol and ethanol extracts of mango seed against 25 representatives gram positive, gram negative, acid fast bacteria and fungi. Mango fruit seed were extracted by Soxhlet using methanol and ethanol as solvents. The extracts were tested against the microorganisms using disc diffusion method at different concentrations: 5 mg/mL, 3.75 mg/mL, 3.125 mg/mL, 2.5 mg/mL, 1.875 mg/mL and 1.25 mg/mL). In vitro antibacterial activities of methanol and ethanol extracts of mango bulb showed inhibitions to tested organisms with variable inhibition zones. Except one organism (Rhodococcus equi), no resistance among the tested strains was shown. The mean zone of inhibition produced ranged between 5 mmand18 mmwith18 mm/Mycobacterium smegmatis showed the highest zone of inhibition. In most test strains comparable zones of inhibitions were noted for both methanol and ethanol extract. Candida albicans and Aspergilllus niger were both inhibited by the extracts. The methanol and ethanol extracts of mango seed showed good inhibitory effects against almost all tested strains. The inhibition zones produced by mango extract were less than those produced by standard positive control drug. This could be due to low diffusion rate of mango extract in agarose medium, a thing needed to be further investigated. The products are potential new antimicrobial therapy in the ethnopharmacology domain.展开更多
杧果大茎点菌Macrophoma mangiferae Hingoroai et Sharma是引起广西杧果象牙品种果腐病的病原菌之一。对杧果大茎点菌和罹病果肉组织,运用病理组织解剖方法,观察到杧果大茎点菌M.mangiferae的子实体在果肉组织中生长发育过程,需经过营...杧果大茎点菌Macrophoma mangiferae Hingoroai et Sharma是引起广西杧果象牙品种果腐病的病原菌之一。对杧果大茎点菌和罹病果肉组织,运用病理组织解剖方法,观察到杧果大茎点菌M.mangiferae的子实体在果肉组织中生长发育过程,需经过营养菌丝集结、菌丝球中心组织细胞分化、雏形分生孢子器形成、分生孢子器产生和释放分生孢子等4个阶段。此项研究结果揭示了该类病原菌子实体细胞学发育特点及致病机理。展开更多
Objective:To examine the antidiarrhoeal activity of alcoholic and aqueous seed kernel extract of Mangifera indica(M.indica) on castor oil-induced diarrhoeul activity in Swiss albino mice.Methods:Mango seed kernels wer...Objective:To examine the antidiarrhoeal activity of alcoholic and aqueous seed kernel extract of Mangifera indica(M.indica) on castor oil-induced diarrhoeul activity in Swiss albino mice.Methods:Mango seed kernels were processed and extracted using alcohol and water. Antidiarrhoeal activity of the extracts were assessed using intestinal motility and faecal score methods.Results:Aqueous and alcoholic extracts of M.indica significantly reduced intestinal motility and faecal score in Swiss albino mice.Conclusions:The present study shows the traditional claim on the use of M.indica seed kernel for treating diarrhoea in Southern parts of India.展开更多
AIM:To investigate the effect of aqueous extract from Mangifera indica L.(MIE)on dextran sulfate sodium (DSS)-induced colitis in rats.METHODS:MIE(150 mg/kg)was administered in two different protocols:(1)rectally,over ...AIM:To investigate the effect of aqueous extract from Mangifera indica L.(MIE)on dextran sulfate sodium (DSS)-induced colitis in rats.METHODS:MIE(150 mg/kg)was administered in two different protocols:(1)rectally,over 7 d at the same time as DSS administration;and(2)once daily over 14 d (by oral gavage,7 d before starting DSS,and rectally for 7 d during DSS administration).General observations of clinical signs were performed.Anti-inflammatory activity of MIE was assessed by myeloperoxidase(MPO)activity. Colonic lipid peroxidation was determined by measuring the levels of thiobarbituric acid reactive substances (TBARS).Reduced glutathione(GSH)levels,expression of inflammatory related mediators[inducible isoforms of nitric oxide synthase(iNOS)and cyclooxygenase (COX)-2,respectively]and cytokines[tumor necrosis factor(TNF)-αand TNF receptors 1 and 2]in colonic tissue were also assessed.Interleukin(IL)-6 and TNF-α serum levels were also measured. RESULTS:The results demonstrated that MIE has anti-inflammatory properties by improvement of clinical signs,reduction of ulceration and reduced MPO activity when administered before DSS.In addition,administration of MIE for 14 d resulted in an increase in GSH and reduction of TBARS levels and iNOS,COX-2, TNF-αand TNF R-2 expression in colonic tissue,and a decrease in IL-6 and TNF-αserum levels. CONCLUSION:MIE has anti-inflammatory activity in a DSS-induced rat colitis model and preventive administration(prior to DSS)seems to be a more effective protocol.展开更多
基金Supported by Key Projects of Basic Platform of the National Science and Technology (2005DKA21005 )National Natural Science Foundation-funded Projects (30260086)~~
文摘[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera.[ Conclusion] It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.
文摘芒果畸形病是芒果上的重要病害之一,由镰孢菌侵染引起,其中以Fusarium mangiferae为主要致病菌。该病害诊断困难,且难于有效控制,因此,一旦发生则对芒果生产造成严重威胁。研究基于ISSR分子标记技术,从50条已知引物中筛选得到一条目的引物UBC 888,该引物可稳定扩增出大小为479bp的F.mangiferae特异性条带（Gen Bank Accession No.KJ526382）。根据获得的特异性片段序列设计引物,成功地将ISSR标记转化为SCAR标记,并获得一对SCAR特异性引物（W342,W1772）和一段大小为1 376bp的特异性扩增片段（Gen Bank Accession No.KJ526383）。通过优化特异性引物扩增条件,获得最适退火温度,构建芒果畸形病病原菌F.mangiferae的快速分子检测技术。此技术操作简单,特异性强,可检测真菌DNA的含量最低为10pg,适用于F.mangiferae和田间带菌芒果组织高灵敏度快速检测,为芒果畸形病的早期诊断和及时预防提供可靠理论依据和技术方法。
文摘Objective:To evaluate the antibacterial activity of the methanolic extract of mango(Mangifera indica L) seed kernel.Methods:Chokanan mango seed kernel and seed kernels from assorted mango varieties were collected,cleaned,dried and powered.Crude methanolic extracts of mango seed kernel were analyzed for the phytochemical constituents.The free radical scavenging activity was determined by 2,2-Diphenyl-1-picrylhydrazyl(DPPH) assay.Antibacterial activity was evaluated by disc diffusion assay with three medically important bacterial pathogens such as methicillin resistant Staphylococcus aureus(S.aureus)(MRSA),Escherichia coli(E.coli) and Vibrio vulnificus(V.vulnificus).Results:Qualitative phytochemical analysis indicated the presence of important phytochemical compounds such as glycosides,saponins,flavanoids, tannins and alkaloids.There was no significant difference in the phytochemical content between the single and assorted mango seed kernels.However,the free radical scavenging study indicated that the assorted mango kernels showed slighdy higher activity than the single species(P【0.05). The crude methanolic extract of mango seed kernel at a concentration of 100 mg/mL is found to have potential antimicrobial activity against MRSA and E.coli compared to V.vulnificus. Study on the antibacterial activity also indicated that there was no significant difference in the antibacterial activity of the single and assorted mango seed kernel extracts.Conclusions:The present study conclusively demonstrates the free radical scavenging activity and antibacterial activities of mango seed kernel.In addition,the results also indicated that there is no significant difference in the phytochemical content and biological activity of mango kernels from single and assorted mango varieties.
文摘Objective:To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica(M.indica) and Mucuna urens(M.urens) seeds extracts against some key enzymes(-amylase,-glucosidasc and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro.Methods:Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection.The inhibitory activities of the seeds extracts against-amylase and-glucosidase were estimated using soluble starch and p-nitrophenylglucopyranosidc as their respective substrates.Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme;inhibition of Fe^(2+)-induced lipid peroxidation by extracts was tested in rat pancreas homogenate.Results:The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance,including gallic acid,chlorogenic acid,caffeic acid,ellagic acid,catechin,rutin,quercitrin.quercetin and kaempferol.Extracts of both seeds effectively inhibited-amylase,-glucosidase and aldose reductase activities in a dose-dependent manner,having inhibitory preference for these enzymes in the order of aldose reductase>-glucosidase>-amylase.With lower half-maximal inhibitory concentrations(IC50)against-amylase,-glucosidase,and aldose reductase,M.indica had stronger inhibitory potency against these enzymes than M.urens.Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern,with M.indica being more potent than M.urens.Conclusions:The results obtained provide support for a possible use of M.indica and M.urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2diabetes.
文摘Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase（LOX） inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively.Methanolic extract（MEMI）,successive water extract （SWMI） and ethyl acetate fraction（EMEMI）,n-butanol fraction（BMEMI） and water soluble fraction （WMEMI） of methanolic extract were evaluated along with respective reference standards. Results:In in ritro DPPH radical scavenging activity,the MEMI,EMEMI and BMEMI have offered significant antioxidant activity with IC<sub>50</sub> values of 13.37.3.55 and 14.19μig/mL respectively.Gallic acid,a reference standard showed significant antioxidant activity with IC? value of 1.88 and found to be more potent compared to all the extracts and fractions.In m vitro LOX inhibition assay,the MEMI,EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC<sub>50</sub> values of 96.71.63.21 and 107.44μg/mL respectively.While,reference drug Indometlhacin also offered significant inhibtion against LOX enzyme activity with IC<sub>50</sub> of 57.75.Furthermore,MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity.Conclusions:These findings suggest that the MEMI and F.MEMT possess potent anti-oxidani and anti-inflammatory activities in in vitro conditions.
基金Supported by Ministry of Science and Technology,Government of the Peoples Republic of Bangladesh(Grant No.39.012.002.01.04.018.2014-319)
文摘Objective: To investigate the presence of different phytoconstituents in Mangifera indica(M. indica) peel and evaluate its cytotoxicity to Artemia salina and hypoglycemic potential in Swiss albino mice.Methods: The methanolic extract of M. indica peel was used to determine the presence of phytoconstituents. Brine shrimp lethality bioassay method was followed to determine the cytotoxic potential of plant extract. In the case of hypoglycemic activity, oral administration of extract at 200 and 400 mg/kg and standard glibenclamide at 10 mg/kg was done, followed by determining the percentage of reduction of plasma glucose from the initial level.Results: The methanolic extract of M. indica peel showed the presence of flavonoid,saponin, steroid, tannins, terpenoids, glycosides and alkaloids. In brine shrimp lethality bioassay, the LC_(50) of the extract and standard vincristine sulfate was found to be 2.04 and0.41 mg/mL, respectively. After 90 and 150 min, the methanolic extract at 200 and 400 mg/kg showed prominent plasma glucose reduction of 13.95%, 22.48% and 14.16%,26.18% respectively compared to standard glibenclamide showing 14.90% and 20.67% plasma glucose reduction.Conclusions: This current research affirms prominent cytotoxic and moderate hypoglycemic potential of M. indica peel. Further bioactivity guided isolation of phytoconstituents and investigation on higher animals can lead to development of new drug molecules.
基金the service of cooperation and cultural action of the embassy of France in Dakar for the financial support
文摘Objective:To evaluate the total phenolic contents,antioxidant and antiglycation activities of leaves,barks,roots and kernels from two cultivars of Mangifera indica(Anacardiaceae).Methods:Total phenolic contents were determined by using Folin-Ciocalteu's method.The antioxidant activities were assessed by three different protocols including DPPH,oxygen radical absorbance capacity and iron(Ⅱ)chelation assays.In addition,in vitro bovine serum albumin/D-ribose assay was chosen to evaluate the antiglycation properties of the extracts.Results:All the investigated extracts were found to contain high level of total phenols as well as potent antioxidant activities.Kernel extracts showed the highest total phenol contents and DPPH radical scavenging activities whereas higher oxygen radical absorbance capacity values were observed for leave,root and bark extracts.Besides,extracts from leaves,roots and barks from both cultivars exhibited potent inhibitory effects against the formation of advanced glycation end products,with IC_(50)values lower than the standard positive control aminoguanidine.Conclusions:The potent antiglycation and antioxidative activities of these two Mangifera indica cultivars suggest a possible role in targeting aging,diabetic complications and oxidative stress related diseases.
基金funded by Fundamental Research Grant Scheme(FRGS Vot 1560)Universiti Tun Hussein Onn Malaysia Contract grant(H277&U688)。
文摘Objective:To determine the lead bioactive compound in kernel extract of Mangifera pajang and its anti-cancer activity against human breast cancer cell lines with positive estrogen receptor(MCF-7).Methods:The methanolic extract of dried powder kernel of Mangifera pajang was exposed to column chromatography for isolation.The structural elucidation of the isolated compound was characterized using infrared,nuclear magnetic resonance,mass spectrometry.Furthermore,cytotoxicity,morphological changes,flow cytometry and cell cycle arrest analyses were performed to examine the mechanism of anti-proliferation and apoptosis induced by methyl gallate against MCF-7.Results:One compound was isolated from the methanolic extract of Mangifera pajang kernel and identified as methyl gallate.The flow cytometric results demonstrated induction of apoptosis in MCF-7 cells by three concentrations of methyl gallate.The cell cycle arrest showed a significant(P<0.05)decrease in cell progression at G2/M phase of MCF-7 after treatment with 100μM of methyl gallate.The cell percentage of early and late apoptosis was significant at 10 and 100μM of methyl gallate.Also,methyl gallate treatment induced up-regulation of reactive oxygen species levels in MCF-7 cells with a reduction in superoxide dismutase levels.Conclusions:These findings indicate that isolated methyl gallate from Mangifera pajang kernel extracts induces growth inhibition and apoptosis in MCF-7 cells via up-regulating oxidative stress pathway.
文摘Mango(Mangifera indica L.)is an important fruit crop in tropical and subtropical countries associated with many agronomic and horticultural problems,such as susceptibility to pathogens,including powdery mildew and anthracnose,poor yield and quality,and short shelf life.Conventional breeding techniques exhibit significant limitations in improving mango quality due to the characteristics of long ripening,self-incompatibility,and high genetic heterozygosity.In recent years,much emphasis has been placed on identification of key genes controlling a certain trait through genomic association analysis and directly breeding new varieties through transgene or genotype selection of offspring.This paper reviews the latest research progress on the genome and transcriptome sequencing of mango fruit.The rapid development of genome sequencing and bioinformatics provides effective strategies for identifying,labeling,cloning,and manipulating many genes related to economically important traits.Preliminary verification of the functions of mango genes has been conducted,including genes related to flowering regulation,fruit development,and polyphenol biosynthesis.Importantly,modern biotechnology can refine existing mango varieties to meet the market demand with high economic benefits.
文摘Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this study is to evaluate the acute general toxicity, antioxidant potential and antibacterial activitiy of leave’s aqueous extracts from Lannea microcarpa Engl. and K. Krause and Mangifera indica L. The use of these plants in traditional medicine motivated our choice to lead scientific studies. Methods: The aqueous decoction of the leaves is the form of use recommended by traditional healers. We used the same type of extracts for studies. Acute toxicity was studied in NMRI strain mice, with the dose of 2000 mg/kg body weight, for each plant species. The antioxidant activity is evaluated by the method of reduction of radical DPPH. The phytochemical compounds were detected with specific reagent: Alcaloids with Dragendorff’s reagent, Flavonoids with ammonia (NH<sub>4</sub>OH), Polyphenols and tannins are revelated by ferric chloride (FeCl<sub>3</sub>). Saponosides were revelated by their foaming power property. Bacterial inhibiting activity is tested by measuring the diameters’ inhibition of extracts on reference strains. Results: The aqueous extracts were not toxic at the maximum dose of 2000 mg/kg body weight, for each plant species. The extracts showed an antioxidant activity with an IC50, 20 μg/ml for Lannea microcarpa and 18 μg/ml for Mangifera indica. The extracts showed no bacterial activity on three strains of bacteria tested: Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The phytochemicals we have identified are for L. microcarpa: tannins and phenolics compounds, triterpenes, saponosides. We identified in M. indica’s extracts: tannins and phenolics compounds, triterpenes, saponosides and flavonoids.
文摘Mangifera indica L. is a species of mango in the Anacardiaceae family. It is found in the wild in tropical regions and cultivated varieties have been introduced to other warm regions of the world. This present study aimed to investigate the in vitro antimicrobial activities of methanol and ethanol extracts of mango seed against 25 representatives gram positive, gram negative, acid fast bacteria and fungi. Mango fruit seed were extracted by Soxhlet using methanol and ethanol as solvents. The extracts were tested against the microorganisms using disc diffusion method at different concentrations: 5 mg/mL, 3.75 mg/mL, 3.125 mg/mL, 2.5 mg/mL, 1.875 mg/mL and 1.25 mg/mL). In vitro antibacterial activities of methanol and ethanol extracts of mango bulb showed inhibitions to tested organisms with variable inhibition zones. Except one organism (Rhodococcus equi), no resistance among the tested strains was shown. The mean zone of inhibition produced ranged between 5 mmand18 mmwith18 mm/Mycobacterium smegmatis showed the highest zone of inhibition. In most test strains comparable zones of inhibitions were noted for both methanol and ethanol extract. Candida albicans and Aspergilllus niger were both inhibited by the extracts. The methanol and ethanol extracts of mango seed showed good inhibitory effects against almost all tested strains. The inhibition zones produced by mango extract were less than those produced by standard positive control drug. This could be due to low diffusion rate of mango extract in agarose medium, a thing needed to be further investigated. The products are potential new antimicrobial therapy in the ethnopharmacology domain.
文摘杧果大茎点菌Macrophoma mangiferae Hingoroai et Sharma是引起广西杧果象牙品种果腐病的病原菌之一。对杧果大茎点菌和罹病果肉组织,运用病理组织解剖方法,观察到杧果大茎点菌M.mangiferae的子实体在果肉组织中生长发育过程,需经过营养菌丝集结、菌丝球中心组织细胞分化、雏形分生孢子器形成、分生孢子器产生和释放分生孢子等4个阶段。此项研究结果揭示了该类病原菌子实体细胞学发育特点及致病机理。
文摘Objective:To examine the antidiarrhoeal activity of alcoholic and aqueous seed kernel extract of Mangifera indica(M.indica) on castor oil-induced diarrhoeul activity in Swiss albino mice.Methods:Mango seed kernels were processed and extracted using alcohol and water. Antidiarrhoeal activity of the extracts were assessed using intestinal motility and faecal score methods.Results:Aqueous and alcoholic extracts of M.indica significantly reduced intestinal motility and faecal score in Swiss albino mice.Conclusions:The present study shows the traditional claim on the use of M.indica seed kernel for treating diarrhoea in Southern parts of India.
基金Supported by Spanish Ministry of Education(MEC,SAF0763138)the Instituto de Salud Carlos Ⅲ,Mental Health Research Network,CIBERSAM,and Foundation Santander-UCM(GR58/08)
文摘AIM:To investigate the effect of aqueous extract from Mangifera indica L.(MIE)on dextran sulfate sodium (DSS)-induced colitis in rats.METHODS:MIE(150 mg/kg)was administered in two different protocols:(1)rectally,over 7 d at the same time as DSS administration;and(2)once daily over 14 d (by oral gavage,7 d before starting DSS,and rectally for 7 d during DSS administration).General observations of clinical signs were performed.Anti-inflammatory activity of MIE was assessed by myeloperoxidase(MPO)activity. Colonic lipid peroxidation was determined by measuring the levels of thiobarbituric acid reactive substances (TBARS).Reduced glutathione(GSH)levels,expression of inflammatory related mediators[inducible isoforms of nitric oxide synthase(iNOS)and cyclooxygenase (COX)-2,respectively]and cytokines[tumor necrosis factor(TNF)-αand TNF receptors 1 and 2]in colonic tissue were also assessed.Interleukin(IL)-6 and TNF-α serum levels were also measured. RESULTS:The results demonstrated that MIE has anti-inflammatory properties by improvement of clinical signs,reduction of ulceration and reduced MPO activity when administered before DSS.In addition,administration of MIE for 14 d resulted in an increase in GSH and reduction of TBARS levels and iNOS,COX-2, TNF-αand TNF R-2 expression in colonic tissue,and a decrease in IL-6 and TNF-αserum levels. CONCLUSION:MIE has anti-inflammatory activity in a DSS-induced rat colitis model and preventive administration(prior to DSS)seems to be a more effective protocol.
