By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mand...By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mandelonitrile into (R)-(?)-mandelic acid, with an enantiomeric excess of >99.9%.展开更多
A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4...A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAKIC (250 mm×4.6 mm, 5 μm). Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS )=2.21, RS =2.14, and RS =3.70, respectively). In contrast, the enantioselectivities between CHIRALPAKIC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.展开更多
Recently, the Hong-Ou-Mandel （HOM） interference between two independent weak coherent pulses （WCPs） has been paid much attention due to the measurement-device-independent （MDI） quantum key distribution （QKD）. ...Recently, the Hong-Ou-Mandel （HOM） interference between two independent weak coherent pulses （WCPs） has been paid much attention due to the measurement-device-independent （MDI） quantum key distribution （QKD）. Using classical wave theory, articles reported before show that the visibility of this kind of HOM-type interference is 〈 50%. In this work, we analyze this kind of interference using quantum optics, which reveals more details compared to the wave theory. Analyses confirm the maximum visibility of 50%. And we conclude that the maximum visibility of 50% comes from the two single-photon states in WCPs, without considering the noise. In the experiment, we successfully approach the visibility of 50% by using WCPs splitting from the single pico-second laser source and phase scanning. Since this kind of HOM interference is immune to slow phase fluctuations, both the realized and proposed experiment designs can provide stable ways of high-resolution optical distance detection.展开更多
Soy protein isolate(SPI)biopolymeric films were prepared by adding different contents of mandelic acid(1 to 5%wrt SPI)to glycerol plasticized SPI by solution casting method.Also,SPI was fermented by Bacillus subtilis ...Soy protein isolate(SPI)biopolymeric films were prepared by adding different contents of mandelic acid(1 to 5%wrt SPI)to glycerol plasticized SPI by solution casting method.Also,SPI was fermented by Bacillus subtilis to get fermented SPI films by solution casting.Molecular mass determination of mandelic acid incorporated and fermented SPI films was carried out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE).Mandelic acid incorporated and fermented SPI films were characterized by Fourier-transform infrared spectroscopy(FT-IR),dynamic mechanical analysis(DMA),tensile strength,water uptake and optical transmittance studies.Results indicated that incorporation of mandelic acid in SPI resulted in high tensile strength(8.03 MPa)and highα-relaxation(Tα)as well as low water uptake.On the other hand,films cannot be prepared from fermented SPI with SPI contents of 8%and 12%.However,film from fermented SPI with 16%SPI content could be prepared but it exhibited low tensile strength(3.18 MPa)and low Tαas well as high water uptake.The resulting mandelic acid incorporated SPI films were also subjected to antimicrobial studies.At all the concentration of mandelic acid,we can easily observe the antimicrobial effect in mandelic acid incorporated SPI films unlike fermented SPI films.This work will be helpful in fabricating antimicrobial SPI film from renewable resources.展开更多
Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmo...Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmol/L substrate, p H 6.0, 2 h at 40 ℃）, this esterase can hydrolyze racemic methyl mandelate to（ R）-methyl mandelate with very high optical purity（e. e. 〉99%） and yield（nearly 90%）. Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate（ S）-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the fi nal chiral product（R）-methyl mandelate, which can potentially simplify the production procedure of（ R）-methyl mandelate catalyzed by esterase.展开更多
Based on the chiral ligand exchange, the distribution behavior of mandelic acid enantiomers, and the partition of Cu2+ at different pH values were studied in a water/alcohol two-phase system containing Cu2+ and N-n-（...Based on the chiral ligand exchange, the distribution behavior of mandelic acid enantiomers, and the partition of Cu2+ at different pH values were studied in a water/alcohol two-phase system containing Cu2+ and N-n-（dodecyl-L-proline（A）.） The influences of the solvent sort, the pH value, the concentrations of Cu2+ and chiral ligand on the partition coefficient（K） and separation factor（α） were discussed. The experimental results show that the A formed has more stable ternary complex with D-mandelic acid enantiomer than with L-mandelic acid enantiomer. There is an important influence of the pH value on K and α. When the pH values are less than 3.5, the formation of binary complexes is thermodynamically unfavourable. K and α become maximum when pH values are above 3.5 and the molar ratio of the chiral ligand to Cu2+ is 2∶1.展开更多
The Eu^3＋ complexes with S（＋）-mandelic acid were synthesized in the form of powders by mixing aqueous solutions of EuCl3, S（＋）-mandelic acid and NaOH in different molar ratios. The powders were characterized by...The Eu^3＋ complexes with S（＋）-mandelic acid were synthesized in the form of powders by mixing aqueous solutions of EuCl3, S（＋）-mandelic acid and NaOH in different molar ratios. The powders were characterized by elemental analysis, X-ray powder diffraction （XRPD.） method, Fourier transform infrared （FTIR） and Raman spectroscopy, UV-vis reflectance and luminescence spectra as well as luminescence lifetime measurements. It was found that all studied powders of Eu^3＋ complexes with S（＋）-mandelic acid were isostructural and crystalline and formed compounds with the formula Eu（Man）3（H2O）2.展开更多
The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547（Aeropyrum pernix esterase 1547）. APE 1547 used in this reaction showed a remarkable stereodi...The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547（Aeropyrum pernix esterase 1547）. APE 1547 used in this reaction showed a remarkable stereodi-scrimination in favour of R-mandelic acid（99% e.e.） with an enantiomeric ratio E〉200. The results of computer simulation are consistent with the experimental results. It can be inferred that the R-substrate adopted a binding mode productive of the reaction due to the formation of the hydrogen bond at the active site of APE 1547.展开更多
The racemic phenylalanine has been separated by (R)-mandelic acid through the formation of diastereomeric molecular complex. The crystal of the title chiral complex (C8H8O3 C9H11NO2, Mr = 317.33) belongs to monoclinic...The racemic phenylalanine has been separated by (R)-mandelic acid through the formation of diastereomeric molecular complex. The crystal of the title chiral complex (C8H8O3 C9H11NO2, Mr = 317.33) belongs to monoclinic, space group C2 with a = 19.391(3), b = 5.715(4), c = 15.755(3) ? b = 115.23(1), V = 1579(1) 3, Z = 4, Dc = 1.335 g/cm3, F(000) = 672, m = 0.099 mm-1, R = 0.033 and wR = 0.060 for 1278 observed reflections (I > 2s(I)). The complex consists of (R)-mandelic acid and (R)-phenylalanine in 1:1 molar ratio, and the complex molecules form layered crystal structure by self-assembly through intermolecular H-bonding between carboxyl and carboxylate of the neighboring molecules.展开更多
We present a two-photon interference experiment in a modified Mach-Zehnder （MZ） interferometer in which two Hong-Ou-Mandel effects occur in tandem and construct superposed two-photon states. The signal photons pass ...We present a two-photon interference experiment in a modified Mach-Zehnder （MZ） interferometer in which two Hong-Ou-Mandel effects occur in tandem and construct superposed two-photon states. The signal photons pass both the arms of the MZ interferometer while the idler photons pass one arm only. Interestingly, the probability of the idler photons emerging from any output port still shows a sine oscillation with the two-photon phase difference and it can be characterized only by the indistinguishability of the two-photon amplitudes. We also observe a two-photon interference pattern with a period being equal to the wavelength of the parametric photons instead of the two-photon photonie de Broglie wavelength due to the presence of two-photon phase difference, in particular, with complementary probabilities of finding the two-photon pairs in two output ports. The abundant observations can facilitate a more comprehensive understanding of the two-photon interference.展开更多
This paper presents a new chiral separation technology:two-phase(O/W)recognition chiral extraction.Distribution behavior of alpha-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-iso...This paper presents a new chiral separation technology:two-phase(O/W)recognition chiral extraction.Distribution behavior of alpha-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-isobutyl tartrate in 1,2-dichloroethane organic phase and beta-CD derivatives in aqueous phase,and the influence of the kind and concentration of extractant and pH on extraction performance was investigated.The experimental results indicate that two-phase(O/W)recognition chiral extraction is of strong chiral separation ability.HP-^-CD,HE-beta-CD and Me-beta-CD have higher recognition ability for S-CHMA than that for R-CHMA,among which HP-beta-CD has the strongest ability;whereas,D-isobutyl tartrate has reversed recognition ability for them.In the extraction system containing HP-beta-CD and D-isobutyl tartrate,e.e.% of S-CHMA in aqueous phase reached 27.6% by one stage extraction,and the distribution ratio for R-CHMA(k_R)and for S-CHMA(k_S)and separation factor(alpha)are 2.44,0.89 and 2.49,respectively.Meanwhile,pH and concentration of extractant have great effects on chiral separation ability.Two-phase(O/W)recognition chiral extraction has great significance for preparative separation of racemic compounds.展开更多
文摘By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mandelonitrile into (R)-(?)-mandelic acid, with an enantiomeric excess of >99.9%.
文摘A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAKIC (250 mm×4.6 mm, 5 μm). Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS )=2.21, RS =2.14, and RS =3.70, respectively). In contrast, the enantioselectivities between CHIRALPAKIC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.
基金Project supported by the National Basic Research Program of China(Grants Nos.2011CBA00200 and 2011CB921200)the National Natural Science Foundation of China(Grant Nos.61201239,61205118,11304397,and 61475148)the“Strategic Priority Research Program(B)”of the Chinese Academy of Sciences(Grant No.XDB01030100 and XDB01030300)
文摘Recently, the Hong-Ou-Mandel （HOM） interference between two independent weak coherent pulses （WCPs） has been paid much attention due to the measurement-device-independent （MDI） quantum key distribution （QKD）. Using classical wave theory, articles reported before show that the visibility of this kind of HOM-type interference is 〈 50%. In this work, we analyze this kind of interference using quantum optics, which reveals more details compared to the wave theory. Analyses confirm the maximum visibility of 50%. And we conclude that the maximum visibility of 50% comes from the two single-photon states in WCPs, without considering the noise. In the experiment, we successfully approach the visibility of 50% by using WCPs splitting from the single pico-second laser source and phase scanning. Since this kind of HOM interference is immune to slow phase fluctuations, both the realized and proposed experiment designs can provide stable ways of high-resolution optical distance detection.
文摘Soy protein isolate(SPI)biopolymeric films were prepared by adding different contents of mandelic acid(1 to 5%wrt SPI)to glycerol plasticized SPI by solution casting method.Also,SPI was fermented by Bacillus subtilis to get fermented SPI films by solution casting.Molecular mass determination of mandelic acid incorporated and fermented SPI films was carried out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE).Mandelic acid incorporated and fermented SPI films were characterized by Fourier-transform infrared spectroscopy(FT-IR),dynamic mechanical analysis(DMA),tensile strength,water uptake and optical transmittance studies.Results indicated that incorporation of mandelic acid in SPI resulted in high tensile strength(8.03 MPa)and highα-relaxation(Tα)as well as low water uptake.On the other hand,films cannot be prepared from fermented SPI with SPI contents of 8%and 12%.However,film from fermented SPI with 16%SPI content could be prepared but it exhibited low tensile strength(3.18 MPa)and low Tαas well as high water uptake.The resulting mandelic acid incorporated SPI films were also subjected to antimicrobial studies.At all the concentration of mandelic acid,we can easily observe the antimicrobial effect in mandelic acid incorporated SPI films unlike fermented SPI films.This work will be helpful in fabricating antimicrobial SPI film from renewable resources.
基金Supported by the National Natural Science Foundation of China(No.21302199)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11030404)+1 种基金the Project of“Engineering HighPerformance Microorganisms for Advanced Bio-Based Manufacturing”from the Chinese Academy of Sciences(No.KGZD-EW-606)the Guangzhou Science and Technology Plan Projects(No.201510010012)
文摘Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmol/L substrate, p H 6.0, 2 h at 40 ℃）, this esterase can hydrolyze racemic methyl mandelate to（ R）-methyl mandelate with very high optical purity（e. e. 〉99%） and yield（nearly 90%）. Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate（ S）-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the fi nal chiral product（R）-methyl mandelate, which can potentially simplify the production procedure of（ R）-methyl mandelate catalyzed by esterase.
基金Project(20376085) supported by the National Natural Science Foundation of China
文摘Based on the chiral ligand exchange, the distribution behavior of mandelic acid enantiomers, and the partition of Cu2+ at different pH values were studied in a water/alcohol two-phase system containing Cu2+ and N-n-（dodecyl-L-proline（A）.） The influences of the solvent sort, the pH value, the concentrations of Cu2+ and chiral ligand on the partition coefficient（K） and separation factor（α） were discussed. The experimental results show that the A formed has more stable ternary complex with D-mandelic acid enantiomer than with L-mandelic acid enantiomer. There is an important influence of the pH value on K and α. When the pH values are less than 3.5, the formation of binary complexes is thermodynamically unfavourable. K and α become maximum when pH values are above 3.5 and the molar ratio of the chiral ligand to Cu2+ is 2∶1.
文摘The Eu^3＋ complexes with S（＋）-mandelic acid were synthesized in the form of powders by mixing aqueous solutions of EuCl3, S（＋）-mandelic acid and NaOH in different molar ratios. The powders were characterized by elemental analysis, X-ray powder diffraction （XRPD.） method, Fourier transform infrared （FTIR） and Raman spectroscopy, UV-vis reflectance and luminescence spectra as well as luminescence lifetime measurements. It was found that all studied powders of Eu^3＋ complexes with S（＋）-mandelic acid were isostructural and crystalline and formed compounds with the formula Eu（Man）3（H2O）2.
基金Supported by the National Natural Science Foundation of China(Nos.30870539, 21072075)the 38th Postdoctoral Foundation of China(No.801050321413)
文摘The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547（Aeropyrum pernix esterase 1547）. APE 1547 used in this reaction showed a remarkable stereodi-scrimination in favour of R-mandelic acid（99% e.e.） with an enantiomeric ratio E〉200. The results of computer simulation are consistent with the experimental results. It can be inferred that the R-substrate adopted a binding mode productive of the reaction due to the formation of the hydrogen bond at the active site of APE 1547.
基金The work was supported by the National Natural Science Foundation of China (29973036)
文摘The racemic phenylalanine has been separated by (R)-mandelic acid through the formation of diastereomeric molecular complex. The crystal of the title chiral complex (C8H8O3 C9H11NO2, Mr = 317.33) belongs to monoclinic, space group C2 with a = 19.391(3), b = 5.715(4), c = 15.755(3) ? b = 115.23(1), V = 1579(1) 3, Z = 4, Dc = 1.335 g/cm3, F(000) = 672, m = 0.099 mm-1, R = 0.033 and wR = 0.060 for 1278 observed reflections (I > 2s(I)). The complex consists of (R)-mandelic acid and (R)-phenylalanine in 1:1 molar ratio, and the complex molecules form layered crystal structure by self-assembly through intermolecular H-bonding between carboxyl and carboxylate of the neighboring molecules.
基金Supported by the National Key R&D Program of China under Grant Nos 2017YFA0303800 and 2017YFA0303700the National Natural Science Foundation of China under Grant Nos 11534006,11774183 and 11674184+1 种基金the Natural Science Foundation of Tianjin under Grant No 16JCZDJC31300the Collaborative Innovation Center of Extreme Optics
文摘We present a two-photon interference experiment in a modified Mach-Zehnder （MZ） interferometer in which two Hong-Ou-Mandel effects occur in tandem and construct superposed two-photon states. The signal photons pass both the arms of the MZ interferometer while the idler photons pass one arm only. Interestingly, the probability of the idler photons emerging from any output port still shows a sine oscillation with the two-photon phase difference and it can be characterized only by the indistinguishability of the two-photon amplitudes. We also observe a two-photon interference pattern with a period being equal to the wavelength of the parametric photons instead of the two-photon photonie de Broglie wavelength due to the presence of two-photon phase difference, in particular, with complementary probabilities of finding the two-photon pairs in two output ports. The abundant observations can facilitate a more comprehensive understanding of the two-photon interference.
基金Supported by the National Natural Science Foundation of China (Grant No. 20776038)
文摘This paper presents a new chiral separation technology:two-phase(O/W)recognition chiral extraction.Distribution behavior of alpha-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-isobutyl tartrate in 1,2-dichloroethane organic phase and beta-CD derivatives in aqueous phase,and the influence of the kind and concentration of extractant and pH on extraction performance was investigated.The experimental results indicate that two-phase(O/W)recognition chiral extraction is of strong chiral separation ability.HP-^-CD,HE-beta-CD and Me-beta-CD have higher recognition ability for S-CHMA than that for R-CHMA,among which HP-beta-CD has the strongest ability;whereas,D-isobutyl tartrate has reversed recognition ability for them.In the extraction system containing HP-beta-CD and D-isobutyl tartrate,e.e.% of S-CHMA in aqueous phase reached 27.6% by one stage extraction,and the distribution ratio for R-CHMA(k_R)and for S-CHMA(k_S)and separation factor(alpha)are 2.44,0.89 and 2.49,respectively.Meanwhile,pH and concentration of extractant have great effects on chiral separation ability.Two-phase(O/W)recognition chiral extraction has great significance for preparative separation of racemic compounds.
