目的探讨人乳腺珠蛋白(h MAM)m RNA在乳腺癌患者外周血中的表达情况及意义。方法选取87例乳腺癌患者、70例乳腺良性肿瘤患者和50例健康志愿者,采用巢式-逆转录-聚合酶链反应检测其外周血h MAM m RNA的表达水平。结果乳腺癌患者的外周血h...目的探讨人乳腺珠蛋白(h MAM)m RNA在乳腺癌患者外周血中的表达情况及意义。方法选取87例乳腺癌患者、70例乳腺良性肿瘤患者和50例健康志愿者,采用巢式-逆转录-聚合酶链反应检测其外周血h MAM m RNA的表达水平。结果乳腺癌患者的外周血h MAM m RNA阳性率为54.02%,高于乳腺良性肿瘤患者的1.43%和健康志愿者的0,差异均有统计学意义(P﹤0.05);乳腺良性肿瘤患者和健康志愿者的外周血h MAM m RNA阳性率比较,差异无统计学意义(P﹥0.05)。Ⅲ~Ⅳ期、有淋巴结转移的乳腺癌患者的h MAM m RNA阳性率高于Ⅰ~Ⅱ期和无淋巴结转移的患者(P﹤0.05)。乳腺癌患者化疗后的外周血h MAM m RNA阳性率为18.39%,低于化疗前的54.02%(P﹤0.05)。结论乳腺癌患者外周血h MAM m RNA的阳性表达与TNM分期和淋巴结转移情况有关,可为临床诊断和预后判断提供依据。展开更多
Background and Objectives: Breast cancer is among the most common causes of cancer related mortality in women worldwide. Early detection and prompt diagnosis of tumor is the first step to prevent cancer-related morbid...Background and Objectives: Breast cancer is among the most common causes of cancer related mortality in women worldwide. Early detection and prompt diagnosis of tumor is the first step to prevent cancer-related morbidity and mortality, and a comprehensive understanding of the involved molecular mechanisms can greatly help in this respect. Breast cancer, like many other types of cancer, is caused by a combination of genetic and epigenetic changes such as inactivation of tumor suppressor genes. Materials and Methods: This study was performed on 40 breast cancer patients and 40 healthy controls. Quantitative real time reverse transcription polymerase chain reaction (real time qRT-PCR) was used to assess the expression of carcinoembryonic antigen (CEA) and mammaglobin mRNA in the peripheral blood of patients and healthy controls. The two groups were compared using t-test. Results: The two groups were not significantly different in terms of the mean age. Twenty-nine out of 40 cancer patients were positive for CEA mRNA and its sensitivity was calculated to be 72.5%. Twelve out of 40 healthy controls were positive for CEA mRNA. Twenty-six out of 40 patients were positive for mammaglobin mRNA indicative of 65% sensitivity while only five out of 40 healthy controls were positive for mammaglobin mRNA. Conclusion: Both CEA and mammaglobin mRNA had high sensitivity in cancer patients;thus, they can be used for screening and early detection of breast cancer patients. Further studies with larger sample sizes are required to confirm the current findings.展开更多
文摘目的探讨人乳腺珠蛋白(h MAM)m RNA在乳腺癌患者外周血中的表达情况及意义。方法选取87例乳腺癌患者、70例乳腺良性肿瘤患者和50例健康志愿者,采用巢式-逆转录-聚合酶链反应检测其外周血h MAM m RNA的表达水平。结果乳腺癌患者的外周血h MAM m RNA阳性率为54.02%,高于乳腺良性肿瘤患者的1.43%和健康志愿者的0,差异均有统计学意义(P﹤0.05);乳腺良性肿瘤患者和健康志愿者的外周血h MAM m RNA阳性率比较,差异无统计学意义(P﹥0.05)。Ⅲ~Ⅳ期、有淋巴结转移的乳腺癌患者的h MAM m RNA阳性率高于Ⅰ~Ⅱ期和无淋巴结转移的患者(P﹤0.05)。乳腺癌患者化疗后的外周血h MAM m RNA阳性率为18.39%,低于化疗前的54.02%(P﹤0.05)。结论乳腺癌患者外周血h MAM m RNA的阳性表达与TNM分期和淋巴结转移情况有关,可为临床诊断和预后判断提供依据。
文摘Background and Objectives: Breast cancer is among the most common causes of cancer related mortality in women worldwide. Early detection and prompt diagnosis of tumor is the first step to prevent cancer-related morbidity and mortality, and a comprehensive understanding of the involved molecular mechanisms can greatly help in this respect. Breast cancer, like many other types of cancer, is caused by a combination of genetic and epigenetic changes such as inactivation of tumor suppressor genes. Materials and Methods: This study was performed on 40 breast cancer patients and 40 healthy controls. Quantitative real time reverse transcription polymerase chain reaction (real time qRT-PCR) was used to assess the expression of carcinoembryonic antigen (CEA) and mammaglobin mRNA in the peripheral blood of patients and healthy controls. The two groups were compared using t-test. Results: The two groups were not significantly different in terms of the mean age. Twenty-nine out of 40 cancer patients were positive for CEA mRNA and its sensitivity was calculated to be 72.5%. Twelve out of 40 healthy controls were positive for CEA mRNA. Twenty-six out of 40 patients were positive for mammaglobin mRNA indicative of 65% sensitivity while only five out of 40 healthy controls were positive for mammaglobin mRNA. Conclusion: Both CEA and mammaglobin mRNA had high sensitivity in cancer patients;thus, they can be used for screening and early detection of breast cancer patients. Further studies with larger sample sizes are required to confirm the current findings.
