In order to investigate the effect of hepatitis B virus (HBV) and aflatoxin B 1 (AFB 1) on hepatocarcinogenesis, the human embryonic liver cells infected with HBV were transplanted to nude mice by subcutaneous route a...In order to investigate the effect of hepatitis B virus (HBV) and aflatoxin B 1 (AFB 1) on hepatocarcinogenesis, the human embryonic liver cells infected with HBV were transplanted to nude mice by subcutaneous route and the transplanted mice were divided into 4 groups for study, in which the group A of mice was injected with HBV-infected human embryonic liver cells and followed by injections of AFB 1 once a week (HBV+AFB 1); the group B was treated with HBV as group A, but no AFB 1 was given (HBV +); the group C was injected with normal human embryonic liver cells and AFB 1 was used as group (AFB 1 +) and the group D or control group was injected with normal embryonic liver cells without addition of AFB 1. The experimental results showed that the incidences of tumor formation in different groups were 27.3% (6/22) in group A; 0% (0/13) in group B; 13.3% (2/15) in group C and 0% (0/14) in group D respectively. All the tumors formed were proved to be human hepatocellular carcinoma (HCC) by pathological examinations and the tumor tissues were anthrogenetic as demonstrated by EMA monoclonal antibody. The HBV-X and HBV-S genes could be detected in the tumor tissues by means of slot hybridization and PCR amplification, indicating that the HBV-DNA genes had integrated into DNA of host cells. Thus, we have successfully induced the human HCC through HBV infection and introduction of AFB 1 with a synergistic effect between HBV and AFB 1 in hepatocarcinogenesis.展开更多
Background and objective This retrospective study was designed to evaluate the response and survival of malignant pleural mesothelioma to radiotherapy when delivered with surgery and chemotherapy and when delivered al...Background and objective This retrospective study was designed to evaluate the response and survival of malignant pleural mesothelioma to radiotherapy when delivered with surgery and chemotherapy and when delivered alone or with chemotherapy. Methods A study for 110 patients with malignant pleural mesothelioma who presented to radiotherapy department, National Cancer Institute, Cairo and received radiation therapy in the period from January 1999 to July 2007. Results Forty-six patients (41.8%) received trimodality therapy (surgery & adjuvant or neoadjuvant chemotherapy & adjuvant radiotherapy), while bimodality therapy (chemotherapy & radiotherapy) in 38 patients (34.5%), while 26 patients (23.6%) received single modality therapy (palliative radiotherapy), 22 patients (20%) developed local recurrence, 22 patients (20%) developed distant metastases months, 14 patients (12.7%) developed local disease progression, 25 patients (22.7%) are still alive and free of disease at time of reporting. The median survival for all patients was 16 months, while 12 and 18 months overall survival were 63.6% & 31.8% respectively while median survival for stage II, III, IV patients was 16.5, 12.5 and 8 months respectively. Conclusion Multimodality approach involving surgery, chemotherapy and radiotherapy have been evaluated and proved its superiority in improving survival, especially in stages II.展开更多
目的探讨超声造影对胰腺癌及肿块型胰腺炎的鉴别诊断价值,提高术前诊断符合率。方法选取我院经手术及病理确诊的胰腺癌患者32例(胰腺癌组)和肿块型胰腺炎患者28例(肿块型胰腺炎组),均行常规超声和超声造影检查,分析并比较两组超声造影时...目的探讨超声造影对胰腺癌及肿块型胰腺炎的鉴别诊断价值,提高术前诊断符合率。方法选取我院经手术及病理确诊的胰腺癌患者32例(胰腺癌组)和肿块型胰腺炎患者28例(肿块型胰腺炎组),均行常规超声和超声造影检查,分析并比较两组超声造影时间-强度曲线各参数的差异;绘制常规超声和超声造影诊断胰腺良恶性肿块的受试者工作特征(ROC)曲线并计算其曲线下面积,比较两种检查方法对胰腺良恶性肿块的诊断效能。结果超声造影静脉相:胰腺癌组不均质增强比例为77.14%,高于肿块型胰腺炎组(35.71%),差异有统计学意义(P<0.01)。胰腺癌组上升时间、达峰时间、峰值强度分别为2.82 s、3.51 s、58.18 d B,与肿块型胰腺炎组(1.31 s、1.56 s、76.09 d B)比较,差异均有统计学意义(均P<0.05);平均渡越时间比较差异无统计学意义。常规超声诊断胰腺癌及肿块性胰腺炎的ROC曲线下面积为0.544,敏感性为60.07%,特异性为53.12%,诊断符合率为56.66%。超声造影诊断胰腺癌及肿块性胰腺炎的ROC曲线下面积为0.780,敏感性为80.00%,特异性为73.33%,诊断符合率为76.67%,两者比较差异均有统计学意义(均P<0.05)。结论超声造影对于胰腺癌及肿块型胰腺炎的诊断及鉴别诊断较常规超声更加准确和客观,有较高的临床应用价值。展开更多
基金This research was supported in whole with key program from National Natural Science Foundation of China (No. 39830380)
文摘In order to investigate the effect of hepatitis B virus (HBV) and aflatoxin B 1 (AFB 1) on hepatocarcinogenesis, the human embryonic liver cells infected with HBV were transplanted to nude mice by subcutaneous route and the transplanted mice were divided into 4 groups for study, in which the group A of mice was injected with HBV-infected human embryonic liver cells and followed by injections of AFB 1 once a week (HBV+AFB 1); the group B was treated with HBV as group A, but no AFB 1 was given (HBV +); the group C was injected with normal human embryonic liver cells and AFB 1 was used as group (AFB 1 +) and the group D or control group was injected with normal embryonic liver cells without addition of AFB 1. The experimental results showed that the incidences of tumor formation in different groups were 27.3% (6/22) in group A; 0% (0/13) in group B; 13.3% (2/15) in group C and 0% (0/14) in group D respectively. All the tumors formed were proved to be human hepatocellular carcinoma (HCC) by pathological examinations and the tumor tissues were anthrogenetic as demonstrated by EMA monoclonal antibody. The HBV-X and HBV-S genes could be detected in the tumor tissues by means of slot hybridization and PCR amplification, indicating that the HBV-DNA genes had integrated into DNA of host cells. Thus, we have successfully induced the human HCC through HBV infection and introduction of AFB 1 with a synergistic effect between HBV and AFB 1 in hepatocarcinogenesis.
文摘Background and objective This retrospective study was designed to evaluate the response and survival of malignant pleural mesothelioma to radiotherapy when delivered with surgery and chemotherapy and when delivered alone or with chemotherapy. Methods A study for 110 patients with malignant pleural mesothelioma who presented to radiotherapy department, National Cancer Institute, Cairo and received radiation therapy in the period from January 1999 to July 2007. Results Forty-six patients (41.8%) received trimodality therapy (surgery & adjuvant or neoadjuvant chemotherapy & adjuvant radiotherapy), while bimodality therapy (chemotherapy & radiotherapy) in 38 patients (34.5%), while 26 patients (23.6%) received single modality therapy (palliative radiotherapy), 22 patients (20%) developed local recurrence, 22 patients (20%) developed distant metastases months, 14 patients (12.7%) developed local disease progression, 25 patients (22.7%) are still alive and free of disease at time of reporting. The median survival for all patients was 16 months, while 12 and 18 months overall survival were 63.6% & 31.8% respectively while median survival for stage II, III, IV patients was 16.5, 12.5 and 8 months respectively. Conclusion Multimodality approach involving surgery, chemotherapy and radiotherapy have been evaluated and proved its superiority in improving survival, especially in stages II.
文摘目的探讨超声造影对胰腺癌及肿块型胰腺炎的鉴别诊断价值,提高术前诊断符合率。方法选取我院经手术及病理确诊的胰腺癌患者32例(胰腺癌组)和肿块型胰腺炎患者28例(肿块型胰腺炎组),均行常规超声和超声造影检查,分析并比较两组超声造影时间-强度曲线各参数的差异;绘制常规超声和超声造影诊断胰腺良恶性肿块的受试者工作特征(ROC)曲线并计算其曲线下面积,比较两种检查方法对胰腺良恶性肿块的诊断效能。结果超声造影静脉相:胰腺癌组不均质增强比例为77.14%,高于肿块型胰腺炎组(35.71%),差异有统计学意义(P<0.01)。胰腺癌组上升时间、达峰时间、峰值强度分别为2.82 s、3.51 s、58.18 d B,与肿块型胰腺炎组(1.31 s、1.56 s、76.09 d B)比较,差异均有统计学意义(均P<0.05);平均渡越时间比较差异无统计学意义。常规超声诊断胰腺癌及肿块性胰腺炎的ROC曲线下面积为0.544,敏感性为60.07%,特异性为53.12%,诊断符合率为56.66%。超声造影诊断胰腺癌及肿块性胰腺炎的ROC曲线下面积为0.780,敏感性为80.00%,特异性为73.33%,诊断符合率为76.67%,两者比较差异均有统计学意义(均P<0.05)。结论超声造影对于胰腺癌及肿块型胰腺炎的诊断及鉴别诊断较常规超声更加准确和客观,有较高的临床应用价值。
