[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechani...[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.展开更多
基金Supported by Project of Science and Technology Department of Inner Mongolia Autonomous Region
文摘[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.
