Since the introduction of intracytoplasmic sperm injection （ICSI） using single sperm isolated from testicular tissue in men with obstructive and non-obstructive azoospermia, or using ejaculated sperm in those with p...Since the introduction of intracytoplasmic sperm injection （ICSI） using single sperm isolated from testicular tissue in men with obstructive and non-obstructive azoospermia, or using ejaculated sperm in those with poor semen quality, there have been concerns that this might have adverse effects on the offspring compared to conventional in vitrofertilisation （IVF） and natural conceptions. ICSI is done for reasons other than male factor infertility, and on the whole has not been shown to have any more negative effects than those seen with IVF. There have however, been very few studies of ICSI with a focus on, or large enough numbers to examine, the specific outcomes associated with male factor infertility. From the limited information available in relation to the source of the sperm and aetiology of infertility in the presence of ICSI, there appears to be no increased risk of congenital malformations. There is, however, a small increase in both de novoand inherited chromosome abnormalities. In terms of growth and neurodevelopment, there are very few studies, and so far, no adverse outcomes have been found in young children whose fathers have a sperm defect. The origin of the sperm used in ICSI does not have a major influence on the early life outcomes for the offspring, but transgenerational and epigenetic effects remain unknown. When the male factor infertility is known or thought to be due to a Y-chromosome deletion, this information should be given to the young male offspring at a time that will ensure his own reproductive health and plans are optimized.展开更多
Infertility affects 10%–15%of couples worldwide.Of all infertility cases,20%–70%are due to male factors.In the past,men with severe male factor(SMF)were considered sterile.Nevertheless,the development of intracytopl...Infertility affects 10%–15%of couples worldwide.Of all infertility cases,20%–70%are due to male factors.In the past,men with severe male factor(SMF)were considered sterile.Nevertheless,the development of intracytoplasmic sperm injection(ICSI)drastically modified this scenario.The advances in assisted reproductive technology(ART),specifically regarding surgical sperm retrieval procedures,allowed the efficacious treatment of these conditions.Yet,before undergoing ICSI,male factor infertility requires careful evaluation of clinical and lifestyle behavior together with medical treatment.Epidemiologically speaking,women whose male partner is azoospermic tend to be younger and with a better ovarian reserve.These couples,in fact,are proposed ART earlier in their life,and for this reason,their ovarian response after stimulation is generally good.Furthermore,in younger couples,azoospermia can be partially compensated by the efficient ovarian response,resulting in an acceptable fertility rate following in vitro fertilization(IVF)techniques.Conversely,when azoospermia is associated with a reduced ovarian reserve and/or advanced maternal age,the treatment becomes more challenging,with a consequent reduction in IVF outcomes.Nonetheless,azoospermia seems to impair neither the euploidy rate at the blastocyst stage nor the implantation of euploid blastocysts.Based on the current knowledge,the assessment of male infertility factors should involve:(1)evaluation–to diagnose and quantify seminologic alterations;(2)potentiality–to determine the real possibilities to improve sperm parameters and/or retrieve spermatozoa;(3)time–to consider the available“treatment window”,based on maternal age and ovarian reserve.This review represents an update of the definition,prevalence,causes,and treatment of SMF in a modern ART clinic.展开更多
Background: According to the World Health Organization, the worldwide prevalence of infertility is 17.5%. The male share of responsibility is undeniable. Several factors, such as smoking, alcoholism, obesity and envir...Background: According to the World Health Organization, the worldwide prevalence of infertility is 17.5%. The male share of responsibility is undeniable. Several factors, such as smoking, alcoholism, obesity and environmental pollution are sources of infertility in men. The aim of this study was to determine the spermological profile of infertile men and the factors associated with sperm parameter abnormalities. Methods: This retrospective study analysed 1000 sperm samples over an 11-year period, from January 2010 to December 2021. Results: The average age was 37.52 ± 8.66 years. Surgical history of varicocele and teratozoospermia were associated (p-value = 0.0001). Candida albicans was associated with a 2.27-fold risk of necrozoospermia and a 3.14-fold risk of oligozoospermia. The link between the reason for requesting a spermogram and the age range between 38 and 47 was significant (p-value Conclusion: Today, lifestyle and environmental pollution play a major role in sperm parameter abnormalities.展开更多
Primary ciliary dyskinesia （PCD） is an autosomal-recessive disorder resulting from the loss of normal ciliary function. Symptoms include neonatal respiratory distress, chronic sinusitis, bronchiectasis, situs invers...Primary ciliary dyskinesia （PCD） is an autosomal-recessive disorder resulting from the loss of normal ciliary function. Symptoms include neonatal respiratory distress, chronic sinusitis, bronchiectasis, situs inversus, and infertility. However, only 15 PCD-associated genes have been identified to cause male infertility to date. Owing to the genetic heterogeneity of PCD, comprehensive molecular genetic testing is not considered the standard of care. Here, we provide an update of the progress on the identification of genetic factors related to PCD associated with male infertility, summarizing the underlying molecular mechanisms, and discuss the clinical implications of these findings. Further research in this field will impact the diagnostic strategy for male infertility, enabling clinicians to provide patients with informed genetic counseling, and help to adopt the best course of treatment for developing directly targeted personalized medicine.展开更多
Aim: To determine the most common risk factors of male infertility in Mongolian men attending an infertility clinic. Methods: A prospective, case-control study was conducted in which 430 men were enrolled. All the men...Aim: To determine the most common risk factors of male infertility in Mongolian men attending an infertility clinic. Methods: A prospective, case-control study was conducted in which 430 men were enrolled. All the men had sought their first infertility evaluation between 1998-2002 in the State Research Center on Maternal Child Health, Ulaanbaatar, Mongolia. They were divided into two groups depending on the results of their semen analysis: 191 with abnormal semen and 239 with normal semen profile. Univariate and multivariate analyses were performed to determine any association between risk factors and semen abnormality. Results: Logistic regression analysis demonstrated that the testicular volume, a history of sexually transmitted infections (STI), epididymitis and testicular damage all have statistically significant associations with semen abnormality, when controlled for multiple risk factors. Adjusted odds ratios of 3.4 for mumps orchitis, 2.3 for other orchitis and 3.9 for testicular injury were found. Gonorrhoea, the most commonly reported STIs in this study, gave an adjusted odds ratio of 1.0 for having one or more sperm abnormality. An adjusted odds ratio for subjects with a history of other STIs was 2.7. However, as a predictor of azoospermia, STIs had very high odds ratio, being 5.6 in patients with gonorrhoea and 7.6 in patients with other STIs. Conclusion: A history of pathology involving testicular damage appeared to have the strongest impact on male infertility in Mongolia. STIs have less impact on semen quality except when complicated by orchitis, epididymitis and vasal obstruction.展开更多
BACKGROUND： Cerebral infarction is poorly treated due to neuronal necrosis and secondary pathophysiological changes; for example, free radical production and inflammatory reactions. OBJECTIVE： To detect the levels o...BACKGROUND： Cerebral infarction is poorly treated due to neuronal necrosis and secondary pathophysiological changes; for example, free radical production and inflammatory reactions. OBJECTIVE： To detect the levels of high-sensitivity C-reactive protein （hs-CRP）, interleukin-6 （IL-6）, and tumor necrosis factor- a （TNF- α ） in elderly males with cerebral infarction. DESIGN： Non-randomized current control study. SETTING： Cadre Medical Department, Guizhou Provincial People＇s Hospital. PARTICIPANTS： Forty elderly males （65-89 years old） with cerebral infarction were selected from Cadre Medical Department, Guizhou Provincial People＇s Hospital from February 2004 to December 2006. All patients met the diagnostic criteria of cerebral infarction modified at the 4th National Cerebrovascular Disease Academic Meeting, and were diagnosed on the basis of CT or MRI tests. Furthermore, 35 elderly male inpatients （65-87 years old） without cerebral infarction were selected as the control group. Included subjects provided confirmed consent and did not have heart disease, diabetes mellitus, lipid disorder, acute trauma, infection, rheumatism, or other inflammatory diseases. The study was approved by the local ethics committee. There were no significant differences in age, blood pressure, and lipid levels between the cerebral infarction group and the control group （P 〉 0.05）, and this suggested that the baseline data of both groups were comparable. METHODS： Fasting venous blood was drawn from cerebral infarction patients 24 hours after cerebral infarction attack and from control subjects 24 hours after hospitalization. A latex-enhanced immunoturbidimetric assay and an enzyme-linked immunosorbent assay were used to detect the levels of hs-CRP, IL-6, and TNF- α in the serum. MAIN OUTCOME MEASURES： The levels of hs-CRP, 1L-6, and TNF- α in the serum in both groups. RESULTS： Forty cerebral infarction patients and thirty-five control subjects were included in the final analysis without any loss. Levels of hs-CRP, IL-6, and TNF-α in the cerebral infarction group were significantly higher than those in the control group （P 〈 0.01 ）. CONCLUSION： Levels of serum inflammatory reactive factors are increased in elderly males with cerebral infarction.展开更多
Central venous catheterization（CVC）-related venous thrombosis is a common but serious clinical complication, thus prevention and treatment on this problem should be extensively investigated. In this research, we aim...Central venous catheterization（CVC）-related venous thrombosis is a common but serious clinical complication, thus prevention and treatment on this problem should be extensively investigated. In this research, we aimed to investigate the incidence rate of CVC-related venous thrombosis in senile patients and give a further discussion on the related risk factors and predictors. A total of 324 hospitalized senile male patients subjected to CVC were selected. Retrospective investigation and analysis were conducted on age, underlying diseases, clinical medications, catheterization position and side, catheter retention time, and incidence of CVC-related venous thrombosis complications. Basic laboratory test results during catheterization and thrombogenesis were also collected and analyzed. Among the 324 patients, 20 cases（6.17%） of CVC-related venous thrombosis were diagnoseds. The incidence rate of CVC-related venous thrombosis in subclavian vein catheterization was significantly lower than that in femoral vein catheterization（P〈0.01） and that in internal jugular vein catheterization（P〈0.05）. No statistically significant difference was found between femoral vein catheterization and internal jugular vein catheterization（P〉0.05）. Previous venous thrombosis history（P〈0.01）, high lactate dehydrogenase level（P〈0.01）, low high-density lipoprotein（HDL） level（P〈0.05）, and low albumin level（P〈0.05） were found as risk factors or predictors of CVC-related venous thrombosis in senile male patients. Subclavian vein catheterization was the most appropriate choice among senile patients to decrease the incidence of CVC-related venous thrombosis. Previous venous thrombosis history, high lactate dehydrogenase level, low HDL level, and low albumin level were important risk factors in predicting CVC-related venous thrombosis.展开更多
Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men...Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men with proven male infertility, and the other consisted of 150 fertile men with normal semen parameters. Both were matched for age, place of residence and key socio-demographic variables. They were compared for sexual history, past medical and surgical history, past exposures to sexually transmitted infections and treatment, past and current use of drugs as well as smoking and alcohol intake history. Results： Infertile men were significantly more likely than fertile men to report having experienced penile discharge, painful micturition and genital ulcers, less likely to seek treatment for these symptoms and more likely to seek treatment with informal sector providers. Multivariate analysis showed that male infertility was significantly associated with bacteria in semen cultures, self-reporting of previous use of traditional medications and moderate to heavy alcohol intake, but not with smoking and occupational types. Conclusion： Infertility is associated with various proxies of sexually transmitted infections （STIs） and poor healthcare-seeking behavior for STIs in Nigerian men.展开更多
Azoospermia is the complete absence of spermatozoa in the ejaculate in two or more semen analyses after centrifugation.Nonobstructive azoospermia(NOA)represents the most severe form of male factor infertility accounti...Azoospermia is the complete absence of spermatozoa in the ejaculate in two or more semen analyses after centrifugation.Nonobstructive azoospermia(NOA)represents the most severe form of male factor infertility accounting for 10%–15%of cases and stems from an impairment to spermatogenesis.Understanding of the hypothalamic–pituitary–testicular axis has allowed NOA to be subcategorized by anatomic and/or pathophysiologic level.The etiologies of NOA,and therefore,the differential diagnoses when considering NOA as a cause of male factor infertility,can be subcategorized and condensed into several distinct classifications.Etiologies of NOA include primary hypogonadism,secondary hypogonadism,defects in androgen synthesis and/or response,defective spermatogenesis and sperm maturation,or a mixed picture thereof.This review includes up-to-date clinical,diagnostic,cellular,and histologic features pertaining to the multitude of NOA etiologies.This in turn will provide a framework by which physicians practicing infertility can augment their clinical decision-making,patient counseling,thereby improving upon the management of men with NOA.展开更多
Male infertility has seen an increase in prevalence with cases of azoospermia estimated to affect 10%–15%of infertile men.Confirmation of azoospermia subsequently necessitates an early causal differentiation between ...Male infertility has seen an increase in prevalence with cases of azoospermia estimated to affect 10%–15%of infertile men.Confirmation of azoospermia subsequently necessitates an early causal differentiation between obstructive azoospermia(OA)and nonobstructive azoospermia(NOA).Although less common when compared to NOA,OA can represent upward 20%–40%of cases of azoospermia.While there are a multitude of etiologies responsible for causing NOA and OA,correctly distinguishing between the two types of azoospermia has profound implications in managing the infertile male.This review represents an amalgamation of the current guidelines and literature which will supply the reproductive physician with a diagnostic armamentarium to properly distinguish between NOA and OA,therefore providing the best possible care to the infertile couple.展开更多
目的:男性生育力下降与各种因素密切相关,考虑到试管助孕的高成本及治疗失败的负面影响,研究不育男性辅助生殖助孕结局的相关因素是十分必要的。本研究探讨接受辅助生殖治疗不育男性助孕结局的相关危险因素,并进一步建立男性不育患者辅...目的:男性生育力下降与各种因素密切相关,考虑到试管助孕的高成本及治疗失败的负面影响,研究不育男性辅助生殖助孕结局的相关因素是十分必要的。本研究探讨接受辅助生殖治疗不育男性助孕结局的相关危险因素,并进一步建立男性不育患者辅助生殖助孕结局的预测模型。方法:采用方便抽样调查法,选取2023年3月至9月于同济大学附属妇产科医院生殖医学中心行卵细胞质单精子注射-新鲜胚胎移植(intracytoplasmic sperm injectionembryo transfer,ICSI-ET)助孕的1037例不育男性作为研究对象,采用一般资料调查表、焦虑自评量表(Self-rating Anxiety Scale,SAS)、抑郁自评量表(Self-rating Depression Scale,SDS)、中文版知觉压力量表(Chinese Version of Perceived Stress Scale,CPSS)、阿森斯睡眠量表(Athens Insomnia Scale,AIS)收集不育男性的一般资料和临床数据,并通过医院信息系统收集不育男性的精液、内分泌和血液资料,以单周期内是否获得临床妊娠为结局指标。以其配偶是否获得临床妊娠分为获得临床妊娠组(观察组,n=403)与未获得临床妊娠组(对照组,n=634),比较2组患者的自身因素和实验室指标差异,采用Logistic回归分析不育男性助孕结局的危险因素,利用存在统计学差异的因素构建不育男性助孕结局预测模型,通过R软件绘制列线图和受试者操作特征(receiver operating characteristic,ROC)曲线图,并通过ROC曲线的曲线下面积(area under the curve,AUC)、Hosmer-Lemeshow检验、准确率、敏感度、特异度评价预测模型的性能。结果:本研究中共403例(38.86%)不育男性的配偶获得临床妊娠。二元Logistic回归显示:年龄、体重指数、是否吸烟、是否喝酒、每日睡眠时间、每日运动时间、焦虑、抑郁、失眠、前向运动精子率、精子DNA碎片化指数、精子存活率等12个影响因素是不育男性配偶是否获得临床妊娠的独立危险因素(均P<0.05);以此构建的列线图模型显示:AUC为0.975,Hosmer-Lemeshow检验P=0.946,模型的准确率为0.894,敏感度为0.913,特异度为0.884。结论:男性不育患者的助孕结局受到多种因素影响,精子质量和生活方式影响了其助孕结局;本研究构建的预测模型预测效能良好,临床医护人员可根据预测结果制订预见性方案,改善精子质量,缓解负性情绪,改变生活习惯,以便改善辅助生殖技术的助孕结局。展开更多
CCCTC结合因子(CCCTC-binding factor,CTCF)是一种多功能且高度保守的转录调节因子,参与转录调控、染色质结构维护、细胞分化等多种生物学过程.本研究通过RT-qPCR检测CTCF基因在鸡不同组织中的表达量,发现CTCF在脑和心脏中高表达.构建的...CCCTC结合因子(CCCTC-binding factor,CTCF)是一种多功能且高度保守的转录调节因子,参与转录调控、染色质结构维护、细胞分化等多种生物学过程.本研究通过RT-qPCR检测CTCF基因在鸡不同组织中的表达量,发现CTCF在脑和心脏中高表达.构建的CTCF真核过表达载体转染鸡肝癌细胞系LMH(Leghorn Male Hepatoma,LMH)后,成功表达CTCF-Myc融合蛋白.油红O染色和比色法检测显示,过表达CTCF后,LMH细胞脂滴沉积和总甘油三酯(TG)显著增加(P<0.01),磷脂酸磷酸酶1(LIPIN1)基因表达下调(P<0.01),固醇调节元件结合蛋白1(SREBP1)、脂肪酸合酶(FASN)和乙酰辅酶A羧化酶α(ACACA)基因表达显著上调(P<0.05或P<0.01).结果表明,CTCF基因可能通过调控脂质代谢相关基因表达促进脂质沉积.展开更多
文摘Since the introduction of intracytoplasmic sperm injection （ICSI） using single sperm isolated from testicular tissue in men with obstructive and non-obstructive azoospermia, or using ejaculated sperm in those with poor semen quality, there have been concerns that this might have adverse effects on the offspring compared to conventional in vitrofertilisation （IVF） and natural conceptions. ICSI is done for reasons other than male factor infertility, and on the whole has not been shown to have any more negative effects than those seen with IVF. There have however, been very few studies of ICSI with a focus on, or large enough numbers to examine, the specific outcomes associated with male factor infertility. From the limited information available in relation to the source of the sperm and aetiology of infertility in the presence of ICSI, there appears to be no increased risk of congenital malformations. There is, however, a small increase in both de novoand inherited chromosome abnormalities. In terms of growth and neurodevelopment, there are very few studies, and so far, no adverse outcomes have been found in young children whose fathers have a sperm defect. The origin of the sperm used in ICSI does not have a major influence on the early life outcomes for the offspring, but transgenerational and epigenetic effects remain unknown. When the male factor infertility is known or thought to be due to a Y-chromosome deletion, this information should be given to the young male offspring at a time that will ensure his own reproductive health and plans are optimized.
文摘Infertility affects 10%–15%of couples worldwide.Of all infertility cases,20%–70%are due to male factors.In the past,men with severe male factor(SMF)were considered sterile.Nevertheless,the development of intracytoplasmic sperm injection(ICSI)drastically modified this scenario.The advances in assisted reproductive technology(ART),specifically regarding surgical sperm retrieval procedures,allowed the efficacious treatment of these conditions.Yet,before undergoing ICSI,male factor infertility requires careful evaluation of clinical and lifestyle behavior together with medical treatment.Epidemiologically speaking,women whose male partner is azoospermic tend to be younger and with a better ovarian reserve.These couples,in fact,are proposed ART earlier in their life,and for this reason,their ovarian response after stimulation is generally good.Furthermore,in younger couples,azoospermia can be partially compensated by the efficient ovarian response,resulting in an acceptable fertility rate following in vitro fertilization(IVF)techniques.Conversely,when azoospermia is associated with a reduced ovarian reserve and/or advanced maternal age,the treatment becomes more challenging,with a consequent reduction in IVF outcomes.Nonetheless,azoospermia seems to impair neither the euploidy rate at the blastocyst stage nor the implantation of euploid blastocysts.Based on the current knowledge,the assessment of male infertility factors should involve:(1)evaluation–to diagnose and quantify seminologic alterations;(2)potentiality–to determine the real possibilities to improve sperm parameters and/or retrieve spermatozoa;(3)time–to consider the available“treatment window”,based on maternal age and ovarian reserve.This review represents an update of the definition,prevalence,causes,and treatment of SMF in a modern ART clinic.
文摘Background: According to the World Health Organization, the worldwide prevalence of infertility is 17.5%. The male share of responsibility is undeniable. Several factors, such as smoking, alcoholism, obesity and environmental pollution are sources of infertility in men. The aim of this study was to determine the spermological profile of infertile men and the factors associated with sperm parameter abnormalities. Methods: This retrospective study analysed 1000 sperm samples over an 11-year period, from January 2010 to December 2021. Results: The average age was 37.52 ± 8.66 years. Surgical history of varicocele and teratozoospermia were associated (p-value = 0.0001). Candida albicans was associated with a 2.27-fold risk of necrozoospermia and a 3.14-fold risk of oligozoospermia. The link between the reason for requesting a spermogram and the age range between 38 and 47 was significant (p-value Conclusion: Today, lifestyle and environmental pollution play a major role in sperm parameter abnormalities.
文摘Primary ciliary dyskinesia （PCD） is an autosomal-recessive disorder resulting from the loss of normal ciliary function. Symptoms include neonatal respiratory distress, chronic sinusitis, bronchiectasis, situs inversus, and infertility. However, only 15 PCD-associated genes have been identified to cause male infertility to date. Owing to the genetic heterogeneity of PCD, comprehensive molecular genetic testing is not considered the standard of care. Here, we provide an update of the progress on the identification of genetic factors related to PCD associated with male infertility, summarizing the underlying molecular mechanisms, and discuss the clinical implications of these findings. Further research in this field will impact the diagnostic strategy for male infertility, enabling clinicians to provide patients with informed genetic counseling, and help to adopt the best course of treatment for developing directly targeted personalized medicine.
文摘Aim: To determine the most common risk factors of male infertility in Mongolian men attending an infertility clinic. Methods: A prospective, case-control study was conducted in which 430 men were enrolled. All the men had sought their first infertility evaluation between 1998-2002 in the State Research Center on Maternal Child Health, Ulaanbaatar, Mongolia. They were divided into two groups depending on the results of their semen analysis: 191 with abnormal semen and 239 with normal semen profile. Univariate and multivariate analyses were performed to determine any association between risk factors and semen abnormality. Results: Logistic regression analysis demonstrated that the testicular volume, a history of sexually transmitted infections (STI), epididymitis and testicular damage all have statistically significant associations with semen abnormality, when controlled for multiple risk factors. Adjusted odds ratios of 3.4 for mumps orchitis, 2.3 for other orchitis and 3.9 for testicular injury were found. Gonorrhoea, the most commonly reported STIs in this study, gave an adjusted odds ratio of 1.0 for having one or more sperm abnormality. An adjusted odds ratio for subjects with a history of other STIs was 2.7. However, as a predictor of azoospermia, STIs had very high odds ratio, being 5.6 in patients with gonorrhoea and 7.6 in patients with other STIs. Conclusion: A history of pathology involving testicular damage appeared to have the strongest impact on male infertility in Mongolia. STIs have less impact on semen quality except when complicated by orchitis, epididymitis and vasal obstruction.
基金Educational and Technological Foundation for Excellent Talents of Guizhou Province, No. 2005(25)
文摘BACKGROUND： Cerebral infarction is poorly treated due to neuronal necrosis and secondary pathophysiological changes; for example, free radical production and inflammatory reactions. OBJECTIVE： To detect the levels of high-sensitivity C-reactive protein （hs-CRP）, interleukin-6 （IL-6）, and tumor necrosis factor- a （TNF- α ） in elderly males with cerebral infarction. DESIGN： Non-randomized current control study. SETTING： Cadre Medical Department, Guizhou Provincial People＇s Hospital. PARTICIPANTS： Forty elderly males （65-89 years old） with cerebral infarction were selected from Cadre Medical Department, Guizhou Provincial People＇s Hospital from February 2004 to December 2006. All patients met the diagnostic criteria of cerebral infarction modified at the 4th National Cerebrovascular Disease Academic Meeting, and were diagnosed on the basis of CT or MRI tests. Furthermore, 35 elderly male inpatients （65-87 years old） without cerebral infarction were selected as the control group. Included subjects provided confirmed consent and did not have heart disease, diabetes mellitus, lipid disorder, acute trauma, infection, rheumatism, or other inflammatory diseases. The study was approved by the local ethics committee. There were no significant differences in age, blood pressure, and lipid levels between the cerebral infarction group and the control group （P 〉 0.05）, and this suggested that the baseline data of both groups were comparable. METHODS： Fasting venous blood was drawn from cerebral infarction patients 24 hours after cerebral infarction attack and from control subjects 24 hours after hospitalization. A latex-enhanced immunoturbidimetric assay and an enzyme-linked immunosorbent assay were used to detect the levels of hs-CRP, IL-6, and TNF- α in the serum. MAIN OUTCOME MEASURES： The levels of hs-CRP, 1L-6, and TNF- α in the serum in both groups. RESULTS： Forty cerebral infarction patients and thirty-five control subjects were included in the final analysis without any loss. Levels of hs-CRP, IL-6, and TNF-α in the cerebral infarction group were significantly higher than those in the control group （P 〈 0.01 ）. CONCLUSION： Levels of serum inflammatory reactive factors are increased in elderly males with cerebral infarction.
文摘Central venous catheterization（CVC）-related venous thrombosis is a common but serious clinical complication, thus prevention and treatment on this problem should be extensively investigated. In this research, we aimed to investigate the incidence rate of CVC-related venous thrombosis in senile patients and give a further discussion on the related risk factors and predictors. A total of 324 hospitalized senile male patients subjected to CVC were selected. Retrospective investigation and analysis were conducted on age, underlying diseases, clinical medications, catheterization position and side, catheter retention time, and incidence of CVC-related venous thrombosis complications. Basic laboratory test results during catheterization and thrombogenesis were also collected and analyzed. Among the 324 patients, 20 cases（6.17%） of CVC-related venous thrombosis were diagnoseds. The incidence rate of CVC-related venous thrombosis in subclavian vein catheterization was significantly lower than that in femoral vein catheterization（P〈0.01） and that in internal jugular vein catheterization（P〈0.05）. No statistically significant difference was found between femoral vein catheterization and internal jugular vein catheterization（P〉0.05）. Previous venous thrombosis history（P〈0.01）, high lactate dehydrogenase level（P〈0.01）, low high-density lipoprotein（HDL） level（P〈0.05）, and low albumin level（P〈0.05） were found as risk factors or predictors of CVC-related venous thrombosis in senile male patients. Subclavian vein catheterization was the most appropriate choice among senile patients to decrease the incidence of CVC-related venous thrombosis. Previous venous thrombosis history, high lactate dehydrogenase level, low HDL level, and low albumin level were important risk factors in predicting CVC-related venous thrombosis.
文摘Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men with proven male infertility, and the other consisted of 150 fertile men with normal semen parameters. Both were matched for age, place of residence and key socio-demographic variables. They were compared for sexual history, past medical and surgical history, past exposures to sexually transmitted infections and treatment, past and current use of drugs as well as smoking and alcohol intake history. Results： Infertile men were significantly more likely than fertile men to report having experienced penile discharge, painful micturition and genital ulcers, less likely to seek treatment for these symptoms and more likely to seek treatment with informal sector providers. Multivariate analysis showed that male infertility was significantly associated with bacteria in semen cultures, self-reporting of previous use of traditional medications and moderate to heavy alcohol intake, but not with smoking and occupational types. Conclusion： Infertility is associated with various proxies of sexually transmitted infections （STIs） and poor healthcare-seeking behavior for STIs in Nigerian men.
文摘Azoospermia is the complete absence of spermatozoa in the ejaculate in two or more semen analyses after centrifugation.Nonobstructive azoospermia(NOA)represents the most severe form of male factor infertility accounting for 10%–15%of cases and stems from an impairment to spermatogenesis.Understanding of the hypothalamic–pituitary–testicular axis has allowed NOA to be subcategorized by anatomic and/or pathophysiologic level.The etiologies of NOA,and therefore,the differential diagnoses when considering NOA as a cause of male factor infertility,can be subcategorized and condensed into several distinct classifications.Etiologies of NOA include primary hypogonadism,secondary hypogonadism,defects in androgen synthesis and/or response,defective spermatogenesis and sperm maturation,or a mixed picture thereof.This review includes up-to-date clinical,diagnostic,cellular,and histologic features pertaining to the multitude of NOA etiologies.This in turn will provide a framework by which physicians practicing infertility can augment their clinical decision-making,patient counseling,thereby improving upon the management of men with NOA.
文摘Male infertility has seen an increase in prevalence with cases of azoospermia estimated to affect 10%–15%of infertile men.Confirmation of azoospermia subsequently necessitates an early causal differentiation between obstructive azoospermia(OA)and nonobstructive azoospermia(NOA).Although less common when compared to NOA,OA can represent upward 20%–40%of cases of azoospermia.While there are a multitude of etiologies responsible for causing NOA and OA,correctly distinguishing between the two types of azoospermia has profound implications in managing the infertile male.This review represents an amalgamation of the current guidelines and literature which will supply the reproductive physician with a diagnostic armamentarium to properly distinguish between NOA and OA,therefore providing the best possible care to the infertile couple.
文摘目的:男性生育力下降与各种因素密切相关,考虑到试管助孕的高成本及治疗失败的负面影响,研究不育男性辅助生殖助孕结局的相关因素是十分必要的。本研究探讨接受辅助生殖治疗不育男性助孕结局的相关危险因素,并进一步建立男性不育患者辅助生殖助孕结局的预测模型。方法:采用方便抽样调查法,选取2023年3月至9月于同济大学附属妇产科医院生殖医学中心行卵细胞质单精子注射-新鲜胚胎移植(intracytoplasmic sperm injectionembryo transfer,ICSI-ET)助孕的1037例不育男性作为研究对象,采用一般资料调查表、焦虑自评量表(Self-rating Anxiety Scale,SAS)、抑郁自评量表(Self-rating Depression Scale,SDS)、中文版知觉压力量表(Chinese Version of Perceived Stress Scale,CPSS)、阿森斯睡眠量表(Athens Insomnia Scale,AIS)收集不育男性的一般资料和临床数据,并通过医院信息系统收集不育男性的精液、内分泌和血液资料,以单周期内是否获得临床妊娠为结局指标。以其配偶是否获得临床妊娠分为获得临床妊娠组(观察组,n=403)与未获得临床妊娠组(对照组,n=634),比较2组患者的自身因素和实验室指标差异,采用Logistic回归分析不育男性助孕结局的危险因素,利用存在统计学差异的因素构建不育男性助孕结局预测模型,通过R软件绘制列线图和受试者操作特征(receiver operating characteristic,ROC)曲线图,并通过ROC曲线的曲线下面积(area under the curve,AUC)、Hosmer-Lemeshow检验、准确率、敏感度、特异度评价预测模型的性能。结果:本研究中共403例(38.86%)不育男性的配偶获得临床妊娠。二元Logistic回归显示:年龄、体重指数、是否吸烟、是否喝酒、每日睡眠时间、每日运动时间、焦虑、抑郁、失眠、前向运动精子率、精子DNA碎片化指数、精子存活率等12个影响因素是不育男性配偶是否获得临床妊娠的独立危险因素(均P<0.05);以此构建的列线图模型显示:AUC为0.975,Hosmer-Lemeshow检验P=0.946,模型的准确率为0.894,敏感度为0.913,特异度为0.884。结论:男性不育患者的助孕结局受到多种因素影响,精子质量和生活方式影响了其助孕结局;本研究构建的预测模型预测效能良好,临床医护人员可根据预测结果制订预见性方案,改善精子质量,缓解负性情绪,改变生活习惯,以便改善辅助生殖技术的助孕结局。
文摘CCCTC结合因子(CCCTC-binding factor,CTCF)是一种多功能且高度保守的转录调节因子,参与转录调控、染色质结构维护、细胞分化等多种生物学过程.本研究通过RT-qPCR检测CTCF基因在鸡不同组织中的表达量,发现CTCF在脑和心脏中高表达.构建的CTCF真核过表达载体转染鸡肝癌细胞系LMH(Leghorn Male Hepatoma,LMH)后,成功表达CTCF-Myc融合蛋白.油红O染色和比色法检测显示,过表达CTCF后,LMH细胞脂滴沉积和总甘油三酯(TG)显著增加(P<0.01),磷脂酸磷酸酶1(LIPIN1)基因表达下调(P<0.01),固醇调节元件结合蛋白1(SREBP1)、脂肪酸合酶(FASN)和乙酰辅酶A羧化酶α(ACACA)基因表达显著上调(P<0.05或P<0.01).结果表明,CTCF基因可能通过调控脂质代谢相关基因表达促进脂质沉积.
