Waterlogging strongly affects agronomic performance of maize (Zea mays L.). In order to investigate the suitable selection criteria of waterflooding tolerant genotypes, and identify the most susceptible stage and th...Waterlogging strongly affects agronomic performance of maize (Zea mays L.). In order to investigate the suitable selection criteria of waterflooding tolerant genotypes, and identify the most susceptible stage and the best continuous treatment time to waterlogging, 20 common maize inbred lines were subjected to successive artificial waterflooding at seedling stage, and waterlogging tolerance coefficient (WTC) was used to screen waterflooding tolerant genotypes. In addition, peroxidase (POD) activities and malondialdehyde (MDA) contents were measured for 6 of 20 lines. The results showed that the second leaf stage (V2) was the most susceptible stage, and 6 d after waterflooding was the best continuous treatment time. Dry weight (DW) of both shoots and roots of all lines were significantly reduced at 6 d time-point of waterlogging, compared to control. POD activities and MDA contents were negatively and significantly correlated, and the correlation coefficient was -0.9686 (P 〈 0.0001). According to the results, WTC of shoot DW can be used for practical screening as a suitable index, which is significantly different from control and waterlogged plants happened 6 d earlier. Furthermore, leaf chlorosis, MDA content and POD activities could also be used as reference index for material screening. The implications of the results for waterlogging-tolerant material screening and waterlogging-tolerant breeding have been discussed in maize.展开更多
Haploid breeding can shorten the breeding process and improve the breeding efficiency. Currently, in vivo haploid induction technology has been com- monly used in maize ( Zea mays L. ). This paper briefly introduced...Haploid breeding can shorten the breeding process and improve the breeding efficiency. Currently, in vivo haploid induction technology has been com- monly used in maize ( Zea mays L. ). This paper briefly introduced in vivo haploid induction technologies, summarized doubling methods of maize haploids and described the significance and application of maize haploids, which provided the basis for further development of haploid breeding in maize.展开更多
To check the performance of maize under different tillage practices and varied sowing methods, an experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad. The experiment was carried ...To check the performance of maize under different tillage practices and varied sowing methods, an experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad. The experiment was carried out in a randomized complete block design (RCBD) with split plot arrangement with three replications. Different tillage practices viz, conventional tillage, minimum tillage and deep tillage were kept in main plots while sowing methods viz, flat sowing, ridge sowing and bed sowing were allocated to subplots. The maize hybrid “Pioneer-32T16” was sown on 24th February, 2012. Data pertaining to morphological and phenological attributes of maize showed that tillage practices and sowing methods had a significant effect on Germination count·m-2 (7.8), leaf area per plant (5010 cm2), leaf area index (4.87), crop growth rate (20.69 g·m-2·d-1), plant height (213.04 cm), number of leaves per plant (11.89), days to 50% tasseling (72.44) and days to 50% silking (73.77). Economically, maize sown on ridges under deep tillage gave maximum net income of Rs. 85162 ha-1 while minimum net income of Rs. 56984 ha-1 was found where flat sowing was adopted under minimum tillage system and also more BCR of 1.70 was recorded in ridge sown maize under deep tillage.展开更多
The productivity of maize in Eritrea in general and under semi-arid tropics of Hamelmalo in particular is low because of moisture stress. The low moisture content is ascribed to the low and erratic distribution of rai...The productivity of maize in Eritrea in general and under semi-arid tropics of Hamelmalo in particular is low because of moisture stress. The low moisture content is ascribed to the low and erratic distribution of rainfall, high temperature, lack of suitable varieties, and competition by weeds and low soil fertility. To overcome some of these problems, a field experiment was carried out to assess the effect of moisture conservation methods (MCM) and plant density on the productivity of two maize (Zea mays L.) varieties under semi-arid tropics of Hamelmalo, Eritrea. The experiment was conducted in split-split plot design with three MCM viz tied ridge, ridge and furrow and flat-bed in main plots;two maize varieties viz early local and 04sadve hybrid in sub plots and three plant densities by manipulating the plant to plant distance viz 35 cm, 25 cm and 15 cm at a fixed 75 cm row spacing in sub-sub plots, each replicated thrice. The experiment was focused in addressing the effective moisture conservation techniques, optimum plant density to each variety thus to improve productivity. The crop experienced 10°C to 34.8°C minimum and maximum temperature, respectively and received 429.1 mm total rainfall. The results of the experiment indicated that among all the combinations, 04sadve hybrid variety sown at 75 cm × 25 cm spacing in ridge and furrow method or at 75 cm × 15 cm spacing in tied ridge or flat-bed method and early local variety sown at 75 cm × 15 cm spacing in flat-bed being statistically at par resulted in significantly higher moisture conservation and consequently higher grain yield (4509 kg·ha-1) and higher water use efficiency. It is, therefore, recommended that tied ridge or flat-bed of moisture conservation method at 15 cm plant spacing and 04sadve is preferable to optimize productivity in Hamelmalo area, Eritrea.展开更多
In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detect...In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detection method for the genetically modified( GM) maize MON88017 using real-time fluorescent quantitative PCR. Meanwhile,the method is evaluated by several methodological indicators such as specificity,sensitivity,accuracy and uncertainty of measurement. The results show that the method has strong specificity in analysis of genetically modified maize MON88017. The mean value(1. 54%) repeatedly measured for 29 times with the relative deviation of 2. 7% was close to the real value(1. 50%) and the variation coefficient of the measured value was 0. 1. The tested recovery rate is 100% and the uncertainty of measurement is 0. 096. 5 copies of the MON88017 molecular fragment can be detected at 97. 5% confidence level. Consequently,the quantitative detection method established in this paper for the GM maize MON88017 has fairly high specificity,accuracy and sensitivity and this technology established in this paper can provide good technical support for the safety supervision of genetically modified organisms in China.展开更多
In this study,the entropy weight method was used to comprehensively evaluate the eight sweet-glutinous maize varieties in the regional trials in Shanxi Province in 2016. The results showed that the order of the perfor...In this study,the entropy weight method was used to comprehensively evaluate the eight sweet-glutinous maize varieties in the regional trials in Shanxi Province in 2016. The results showed that the order of the performance of comprehensive traits of the tested varieties was Heitiannuo 631,Heitiannuo No. 2,Cainuo No. 5,Xinnuo 8601,FYN 1601,Xinnuo 8608,Jindannuo 41,Caitiannuo 1958( from good to poor). Among them,Heitiannuo 631,Heitiannuo No. 2 and Cainuo No. 5 are excellent varieties,and the rest are good varieties.展开更多
The sensor system is one of the modern and important methods of irrigation management in arid and semi-arid areas, which is water as the limiting factor for crop production. The study was applied for 2016 and 2017 sea...The sensor system is one of the modern and important methods of irrigation management in arid and semi-arid areas, which is water as the limiting factor for crop production. The study was applied for 2016 and 2017 seasons out in Al-Yousifya, 15 km Southwest of Baghdad. A study was conducted to evaluate coefficient uniformity, uniformity distribution and application efficiency for furrow, surface drip and subsurface drip irrigation methods and it was (98, 97 and 89)% and (97, 96 and 88)% for 2016 and 2017 seasons;respectively. And control the volumetric moisture content according to the rhizosphere depth for depths of 10, 20 and 30 cm by means of the sensor system. The results indicated that the height consumptive water use of furrow 707.91 and 689.69 mm<span style="white-space:nowrap;">·</span>season<sup>-1</sup> and the lowest for subsurface drip with emitter deep at 20 cm 313.93 and 293.50 mm<span style="white-space:nowrap;">·</span>season<sup>-1</sup> for 2016 and 2017 seasons;respectively. As well, the highest value of water use efficiency for subsurface in drip irrigation at a depth of 20 cm, was 2.71 and 2.99 kg<span style="white-space:nowrap;">·</span>m<sup>-3</sup> and the lowest value for furrow irrigation was 1.12 and 1.20 kg<span style="white-space:nowrap;">·</span>m<sup>-3</sup> for the 2016 and 2017 seasons;respectively.展开更多
基金supported by the Natural Science Foundation of Hubei Province, China (2008CDB079)the National High Technology Research and Development Program of China (863 Program,2006AA100103)
文摘Waterlogging strongly affects agronomic performance of maize (Zea mays L.). In order to investigate the suitable selection criteria of waterflooding tolerant genotypes, and identify the most susceptible stage and the best continuous treatment time to waterlogging, 20 common maize inbred lines were subjected to successive artificial waterflooding at seedling stage, and waterlogging tolerance coefficient (WTC) was used to screen waterflooding tolerant genotypes. In addition, peroxidase (POD) activities and malondialdehyde (MDA) contents were measured for 6 of 20 lines. The results showed that the second leaf stage (V2) was the most susceptible stage, and 6 d after waterflooding was the best continuous treatment time. Dry weight (DW) of both shoots and roots of all lines were significantly reduced at 6 d time-point of waterlogging, compared to control. POD activities and MDA contents were negatively and significantly correlated, and the correlation coefficient was -0.9686 (P 〈 0.0001). According to the results, WTC of shoot DW can be used for practical screening as a suitable index, which is significantly different from control and waterlogged plants happened 6 d earlier. Furthermore, leaf chlorosis, MDA content and POD activities could also be used as reference index for material screening. The implications of the results for waterlogging-tolerant material screening and waterlogging-tolerant breeding have been discussed in maize.
基金Supported by Project of Modern Crop Breeding[Guangdong Finance of Agriculture(2014)No.492]2012 Innovation and Entrepreneurship Training Program for University Students of Guangdong Province(1134712062)
文摘Haploid breeding can shorten the breeding process and improve the breeding efficiency. Currently, in vivo haploid induction technology has been com- monly used in maize ( Zea mays L. ). This paper briefly introduced in vivo haploid induction technologies, summarized doubling methods of maize haploids and described the significance and application of maize haploids, which provided the basis for further development of haploid breeding in maize.
文摘To check the performance of maize under different tillage practices and varied sowing methods, an experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad. The experiment was carried out in a randomized complete block design (RCBD) with split plot arrangement with three replications. Different tillage practices viz, conventional tillage, minimum tillage and deep tillage were kept in main plots while sowing methods viz, flat sowing, ridge sowing and bed sowing were allocated to subplots. The maize hybrid “Pioneer-32T16” was sown on 24th February, 2012. Data pertaining to morphological and phenological attributes of maize showed that tillage practices and sowing methods had a significant effect on Germination count·m-2 (7.8), leaf area per plant (5010 cm2), leaf area index (4.87), crop growth rate (20.69 g·m-2·d-1), plant height (213.04 cm), number of leaves per plant (11.89), days to 50% tasseling (72.44) and days to 50% silking (73.77). Economically, maize sown on ridges under deep tillage gave maximum net income of Rs. 85162 ha-1 while minimum net income of Rs. 56984 ha-1 was found where flat sowing was adopted under minimum tillage system and also more BCR of 1.70 was recorded in ridge sown maize under deep tillage.
文摘The productivity of maize in Eritrea in general and under semi-arid tropics of Hamelmalo in particular is low because of moisture stress. The low moisture content is ascribed to the low and erratic distribution of rainfall, high temperature, lack of suitable varieties, and competition by weeds and low soil fertility. To overcome some of these problems, a field experiment was carried out to assess the effect of moisture conservation methods (MCM) and plant density on the productivity of two maize (Zea mays L.) varieties under semi-arid tropics of Hamelmalo, Eritrea. The experiment was conducted in split-split plot design with three MCM viz tied ridge, ridge and furrow and flat-bed in main plots;two maize varieties viz early local and 04sadve hybrid in sub plots and three plant densities by manipulating the plant to plant distance viz 35 cm, 25 cm and 15 cm at a fixed 75 cm row spacing in sub-sub plots, each replicated thrice. The experiment was focused in addressing the effective moisture conservation techniques, optimum plant density to each variety thus to improve productivity. The crop experienced 10°C to 34.8°C minimum and maximum temperature, respectively and received 429.1 mm total rainfall. The results of the experiment indicated that among all the combinations, 04sadve hybrid variety sown at 75 cm × 25 cm spacing in ridge and furrow method or at 75 cm × 15 cm spacing in tied ridge or flat-bed method and early local variety sown at 75 cm × 15 cm spacing in flat-bed being statistically at par resulted in significantly higher moisture conservation and consequently higher grain yield (4509 kg·ha-1) and higher water use efficiency. It is, therefore, recommended that tied ridge or flat-bed of moisture conservation method at 15 cm plant spacing and 04sadve is preferable to optimize productivity in Hamelmalo area, Eritrea.
基金Supported by Standardization System Research Project of Sichuan Provincial Bureau of Quality Supervision(ZYBZ2013-39)
文摘In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detection method for the genetically modified( GM) maize MON88017 using real-time fluorescent quantitative PCR. Meanwhile,the method is evaluated by several methodological indicators such as specificity,sensitivity,accuracy and uncertainty of measurement. The results show that the method has strong specificity in analysis of genetically modified maize MON88017. The mean value(1. 54%) repeatedly measured for 29 times with the relative deviation of 2. 7% was close to the real value(1. 50%) and the variation coefficient of the measured value was 0. 1. The tested recovery rate is 100% and the uncertainty of measurement is 0. 096. 5 copies of the MON88017 molecular fragment can be detected at 97. 5% confidence level. Consequently,the quantitative detection method established in this paper for the GM maize MON88017 has fairly high specificity,accuracy and sensitivity and this technology established in this paper can provide good technical support for the safety supervision of genetically modified organisms in China.
基金Biological Breeding Project of Shanxi Academy of Agricultural Sciences(17yzgc057)
文摘In this study,the entropy weight method was used to comprehensively evaluate the eight sweet-glutinous maize varieties in the regional trials in Shanxi Province in 2016. The results showed that the order of the performance of comprehensive traits of the tested varieties was Heitiannuo 631,Heitiannuo No. 2,Cainuo No. 5,Xinnuo 8601,FYN 1601,Xinnuo 8608,Jindannuo 41,Caitiannuo 1958( from good to poor). Among them,Heitiannuo 631,Heitiannuo No. 2 and Cainuo No. 5 are excellent varieties,and the rest are good varieties.
文摘The sensor system is one of the modern and important methods of irrigation management in arid and semi-arid areas, which is water as the limiting factor for crop production. The study was applied for 2016 and 2017 seasons out in Al-Yousifya, 15 km Southwest of Baghdad. A study was conducted to evaluate coefficient uniformity, uniformity distribution and application efficiency for furrow, surface drip and subsurface drip irrigation methods and it was (98, 97 and 89)% and (97, 96 and 88)% for 2016 and 2017 seasons;respectively. And control the volumetric moisture content according to the rhizosphere depth for depths of 10, 20 and 30 cm by means of the sensor system. The results indicated that the height consumptive water use of furrow 707.91 and 689.69 mm<span style="white-space:nowrap;">·</span>season<sup>-1</sup> and the lowest for subsurface drip with emitter deep at 20 cm 313.93 and 293.50 mm<span style="white-space:nowrap;">·</span>season<sup>-1</sup> for 2016 and 2017 seasons;respectively. As well, the highest value of water use efficiency for subsurface in drip irrigation at a depth of 20 cm, was 2.71 and 2.99 kg<span style="white-space:nowrap;">·</span>m<sup>-3</sup> and the lowest value for furrow irrigation was 1.12 and 1.20 kg<span style="white-space:nowrap;">·</span>m<sup>-3</sup> for the 2016 and 2017 seasons;respectively.