Intervertebral disc （IVD） degeneration is the leading cause of disability with no disease-modifying treatment. IVD degeneration is associated with instable mechanical loading in the spine, but little is known about ...Intervertebral disc （IVD） degeneration is the leading cause of disability with no disease-modifying treatment. IVD degeneration is associated with instable mechanical loading in the spine, but little is known about how mechanical stress regulates nucleus notochordal （NC） cells to maintain IVD homeostasis. Here we report that mechanical stress can result in excessive integrin αvβ6-mediated activation of transforming growth factor beta （TGFβ）, decreased NC cell vacuoles, and increased matrix proteoglycan production, and results in degenerative disc disease （DDD）. Knockout of TGFβ type II receptor （TβRII） or integrin αv in the NC cells inhibited functional activity of postnatal NC cells and also resulted in DDD under mechanical loading. Administration of RGD peptide, TGFβ, and αvβ6-neutralizing antibodies attenuated IVD degeneration. Thus, integrin-mediated activation of TGFβ plays a critical role in mechanical signaling transduction to regulate IVD cell function and homeostasis. Manipulation of this signaling pathway may be a potential therapeutic target to modify DDD.展开更多
Extensive lesions involving the posterior quadrant of the cerebral hemisphere （temporal, parietal, and occipital lobes） induce intractable epilepsy. These patients are potential candidates for surgical treatmenttu. ...Extensive lesions involving the posterior quadrant of the cerebral hemisphere （temporal, parietal, and occipital lobes） induce intractable epilepsy. These patients are potential candidates for surgical treatmenttu. Maintenance of isolated nerve tissue activity after surgery plays a crucial role in the neuroprotective effects of neurosurgery treatment. Disconnection surgery of the posterior quadrant is used to completely isolate nerve fibers, while blood supply at the isolated lobes is maintained. Subsequently, cavities caused by cystic or necrotic nerve tissues should be reduced as much as possible,展开更多
The adaptor protein NUMB is involved in asymmetric division and cell fate determination and recognized as an antagonist of Notch.Previous studies have proved that Notch activation in osteoblasts contributes to a high ...The adaptor protein NUMB is involved in asymmetric division and cell fate determination and recognized as an antagonist of Notch.Previous studies have proved that Notch activation in osteoblasts contributes to a high bone mass. In this study, however, an osteopenic phenotype was found in 9-week-old mice using osteoblastic specific Col1a1–2.3-Cre to ablate both Numb and its homologue Numbl. The trabecular bone mass decreased dramatically while the cortical bone mass was unaffected. Here, the Notch signal was not activated,while the tensin homologue deleted on human chromosome 10(PTEN), which dephosphorylates phosphatidylinositide 3-kinases, was elevated, attenuating protein kinase B(Akt). The ubiquitination assay revealed that NUMB may physiologically promote PTEN ubiquitination in the presence of neural precursor cell-expressed developmentally downregulated protein 4–1. In addition, the deficiency of Numb/Numbl also activated the Hedgehog pathway through GLI1. This process was found to improve the ratio of the receptor activator of nuclear factor-k B ligand to osteoprotegerin, which enhanced the differentiation of osteoclasts and bone resorption. In conclusion, this study provides an insight into new functons of NUMB and NUMBL on bone homeostasis.展开更多
On August 2,the IEA,s World Energy Investment 2018 was issued in Beijing.Global energy investments totalled USD1.8 trillion in 2017,the third consecutive year of decline,and were 2% lower than those in 2016 after taki...On August 2,the IEA,s World Energy Investment 2018 was issued in Beijing.Global energy investments totalled USD1.8 trillion in 2017,the third consecutive year of decline,and were 2% lower than those in 2016 after taking out the price factor.The declines in investment in the power generation sector were most significant,with declines in coal power,hydropower and nuclear power outpacing the increases in investment in photovoltaic power generation.展开更多
Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects m...Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects may yield suboptimal effects due to the complexity of the periodontal inflammatory environment. Herein, stem cell spheroid is encapsulated by interfacial assembly of metal-phenolic network(MPN) nanofilm to form a stem cell microsphere capsule. Specifically, periodontal ligament stem cells(PDLSCs) spheroid was coated with FeⅢ/tannic acid coordination network to obtain spheroid@[FeⅢ-TA] microcapsules. The formed biodegradable MPN biointerface acted as a cytoprotective barrier and exhibited antioxidative, antibacterial and anti-inflammatory activities, effectively remodeling the inflammatory microenvironment and maintaining the stemness of PDLSCs. The stem cell microencapsulation proposed in this study can be applied to multiple stem cells with various functional metal ion/polyphenol coordination, providing a simple yet efficient delivery strategy for stem cell stemness maintenance in an inflammatory environment toward a better therapeutic outcome.展开更多
Flooding tolerance is an important trait for tomato breeding.In this study,we obtained a recessive mutant exhibiting highly enhanced submergence resistance.Phenotypical analyses showed that this resistant to flooding(...Flooding tolerance is an important trait for tomato breeding.In this study,we obtained a recessive mutant exhibiting highly enhanced submergence resistance.Phenotypical analyses showed that this resistant to flooding(rf)mutant displays slightly chlorotic leaves and spontaneous initiation of adventitious roots(ARs)on stems.The mutation was mapped to the phytochromobilin synthase gene AUREA(AU),in which a single amino acid substitution from asparagine to tyrosine occurred.In addition to the classic function of AU in phytochrome and chlorophyll biogenesis in leaves,we uncovered its novel role in mediating AR formation on stems.We further observed temporal coincidence of the two phenotypes in the rf mutant:chlorosis and spontaneous AR formation and revealed that AU functions by maintaining heme homeostasis.Interestingly,our grafting results suggest that heme might play roles in AR initiation via long-distance transport from leaves to stems.Our results present genetic evidence for the involvement of the AU–heme oxygenase-1–heme pathway in AR initiation in tomato.As fruit production and yield in the rf mutant are minimally impacted,the mutation identified in this study may provide a target for biotechnological renovation of tomato germplasm in future breeding.展开更多
"IDC predicts that in 2014,the healthcare informatization will continue to maintain high-speed development,mobile health and clinic data center will become the two hot spots of informatization construction of hos..."IDC predicts that in 2014,the healthcare informatization will continue to maintain high-speed development,mobile health and clinic data center will become the two hot spots of informatization construction of hospitals,and the clinic decision-supporting system will be applied on trial.The policy of multi-sited license of physicians will enable the RHIS(Regional Healthcare Informatization System)to start another construction upsurge,and展开更多
From December 2 to 4,2022,Guangzhou hosted the 19th International Finance Forum(IFF)annual meeting,themed“The Ever-Changing World:Reshaping Our Shared Future.”The event was attended by leaders of world organizations...From December 2 to 4,2022,Guangzhou hosted the 19th International Finance Forum(IFF)annual meeting,themed“The Ever-Changing World:Reshaping Our Shared Future.”The event was attended by leaders of world organizations including the United Nations,the International Monetary Fund,and the World Bank.展开更多
Normal plant growth and development ensures the timely maturity and expected yield of crops.The repair mechanism of genome damage under adverse circumstances is essential for maintaining regular plant growth.Herein,tw...Normal plant growth and development ensures the timely maturity and expected yield of crops.The repair mechanism of genome damage under adverse circumstances is essential for maintaining regular plant growth.Herein,two allelic growth retardation mutants of Chinese cabbage,grm1 and grm2,were obtained from EMS-mutagenized populations of wild-type'FT'isolated-cultured microspores and seeds,respectively.Both mutants stably inherited and exhibited stunted growth with smaller leafy-heads.Genetic analysis and allelism test manifested that the mutated trait was triggered by a same single recessive nuclear gene.Via BSR-Seq,Brgrm1 was mapped to a target region including 20 genes on chromosome A09.Whole-genome re-sequencing revealed that BraA09g024830.3C in grm1 had a single base(A)deletion in the 17th exon,leading to a termination codon(TAG).Genotyping showed that the mutated phenotype co-segregated with the InDel in recombinants of the closest linkage markers.In addition,cloning of BraA09g024830.3C in grm2 found that a base substitution(G-A)occurred in the last base of the 1st intron causing an additional 263-bp retention in coding sequences,which in turn led to a termination codon(TAA).BraA09g024830.3C(BrDDB1A)is a homolog of Arabidopsis thaliana Damaged DNA Binding Protein 1A(DDB1A),a key gene of UV tolerance involved in DNA damage repair.The two mutants exhibited normal plant growth identical with wild-type under an extremely low UV radiation.Our results demonstrated that BrDDB1A contributes to maintaining regular plant growth in Chinese cabbage,which provide insights into elucidating the molecular mechanism underlying Chinese cabbage growth and development.展开更多
Rheumatoid arthritis(RA)is a systemic autoimmune disease in which synovial fibroblasts(SFs)maintain chronic inflammation by secreting proinflammatory mediators,leading to joint destruction.While the role of proinflamm...Rheumatoid arthritis(RA)is a systemic autoimmune disease in which synovial fibroblasts(SFs)maintain chronic inflammation by secreting proinflammatory mediators,leading to joint destruction.While the role of proinflammatory mediators in this process is well-established,the contribution of non-inflammatory regulators in SFs to joint pathology remains poorly understood.In this study,we investigated the non-inflammatory role of SFs in RA using a co-culture model,and found that SFs from RA patients promote apoptosis of human chondrocytes.Mechanistic investigations reveal that SFs can secrete small extracellular vesicles(sEVs),which are taken up by chondrocytes and induce chondrocyte apoptosis in both normal chondrocytes and chondrocytes from patients with RA.sEV-derived miRNA 15-29148 are identified as key signaling molecules mediating the apoptosis effects of chondrocytes.Further studies reveal that SF-derived miRNA 15-29148 targeting CIAPIN1 results in increased chondrocyte apoptosis.We further demonstrate that SF-derived miRNA 15-29148 is transferred to chondrocytes,exacerbating cartilage damage in vivo.Moreover,chondrocyte-specific aptamer-modified polyamidoamine nanoparticles not only ameliorated RA but also prevented its onset.This study suggests that,in RA,the secretion of specific sEV-miRNAs from SFs plays a crucial role in promoting chondrocyte apoptosis,potentially through non-inflammatory regulation,and that sEV-miRNA inhibition in SFs may represent an early preventive treatment strategy for cartilage degradation in RA.展开更多
Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental...Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental stage to adult life.To maintain the optimal functionality of the brain,astroglial cells are particularly committed to reacting to every change in tissue homeostatic conditions,from mild modifications of the physiological environment,a process called astrocyte activation,to the more severe alterations occurring in pathological situations causing astrocyte reactivity or reactive astrogliosis(Escartin et al.,2021).During these reactive states,astrocytes mount an active,progressive response encompassing morphological,molecular,and interactional remodeling,leading to the acquisition of new functions and the loss of others,whose intensity,duration,and reversibility are dependent on the nature of the stimulus and regulated in a context-specific manner.展开更多
Apolipoprotein E has diverse functions in neurons:Apolipoprotein E(ApoE)is a glycoprotein that primarily regulates lipid metabolism and transport in the central nervous system.There are three predominant human ApoE pr...Apolipoprotein E has diverse functions in neurons:Apolipoprotein E(ApoE)is a glycoprotein that primarily regulates lipid metabolism and transport in the central nervous system.There are three predominant human ApoE protein isoforms with cysteine and arginine substitutions at amino acid positions 112 and 158 that impact their lipidation and related functions(Flowers and Rebeck,2020).ApoE2 is characterized by Cys112 and Cys158,ApoE3 by Cys112 and Arg158,whereas ApoE4 contains Arg112 and Arg158.展开更多
As 2024 comes to an end,the pickup in consumption and robust growth in a range of sectors have bolstered confidence that the Chinese economy is right on track to meet the preset growth rate of around five percent.Bene...As 2024 comes to an end,the pickup in consumption and robust growth in a range of sectors have bolstered confidence that the Chinese economy is right on track to meet the preset growth rate of around five percent.Benefiting from a flurry of consumption-stimulating policies and measures,China’s online retail sales surpassed RMB 14 trillion in the January-November period,a year-on-year increase of 7.4 percent.Imports and exports maintained steady growth,climbing by 4.9 percent year-on-year in renminbi terms.Value-added industrial output also expanded 5.8 percent year-on-year.The volume of express delivery parcels reached a new high of 150 billion and is expected to grow higher still given the new-year festivities ahead.展开更多
China’s foreign trade registered steady growthinthefirstfivemonthsof theyeardespitegrowingexternal uncertainties.Thecountry’sforeigntradeofgoods rose 4.1 percent year on year during that time to 12.1 trillion yuan (...China’s foreign trade registered steady growthinthefirstfivemonthsof theyeardespitegrowingexternal uncertainties.Thecountry’sforeigntradeofgoods rose 4.1 percent year on year during that time to 12.1 trillion yuan ($1.75 trillion), data from the General Administration of Customs of China (GACC) showed on June 10.展开更多
In life,we are bound to encounter various difficulties and setbacks.However,it is of great significance to maintain a positive attitude when were facing these challenges.For me,this lesson was deeply impressed upon me...In life,we are bound to encounter various difficulties and setbacks.However,it is of great significance to maintain a positive attitude when were facing these challenges.For me,this lesson was deeply impressed upon me during a particularly tough period.展开更多
If you are to choose an event that can best represent China’s culture,undoubtedly,it will be the Spring Festival,or the Chinese Lunar New Year.It is a cherished occasion for families to get together,extend best wishe...If you are to choose an event that can best represent China’s culture,undoubtedly,it will be the Spring Festival,or the Chinese Lunar New Year.It is a cherished occasion for families to get together,extend best wishes to the people around them,and maintain friendships.展开更多
China’s economy is expected to remain stable throughout 2025.According to projections by the Center for Forecasting Science of the Academy of Mathematics and Systems Science under the Chinese Academy of Sciences,Chin...China’s economy is expected to remain stable throughout 2025.According to projections by the Center for Forecasting Science of the Academy of Mathematics and Systems Science under the Chinese Academy of Sciences,China’s GDP(gross domestic product)growth should settle at around 4.8 percent this year and will likely hit 5 percent if the government’s stimulus package is effectively implemented.I personally believe that the Chinese economy will maintain growth of about 4.6 percent in 2025 with robust performance in the first and fourth quarters and slower economic activity in the second and third quarters.展开更多
The annual Central Economic Work Conference 2024,held on December 11-12,set the overall goal and policy framework for Chinese economy in 2025,a crucial year in China’s economic development as the final year of the 14...The annual Central Economic Work Conference 2024,held on December 11-12,set the overall goal and policy framework for Chinese economy in 2025,a crucial year in China’s economic development as the final year of the 14th Five-Year Plan(FYP)and the preparation for the 15th FYP.A series of incremental fiscal and monetary policies adopted since the end of September has seen their initial effects in stabilizing stock markets and boosting consumption and production and thus enhanced the confidence of meeting the GDP goal for 2024.In the face of complicated external challenges and domestic difficulties-inadequate effective demand,overcapacity in some sectors and weak expectations,it is crucial to maintain stable growth in 2025.The Central Economic Work Conference 2024 set the general goal of 2025 on maintaining stable growth,employment and consumer prices.展开更多
China-Africa relations maintained their upward trajectory in 2024,with new plans for future cooperation taking shape.Chinese President Xi Jinping described the China-Africa partnership as being at its best in history....China-Africa relations maintained their upward trajectory in 2024,with new plans for future cooperation taking shape.Chinese President Xi Jinping described the China-Africa partnership as being at its best in history.The Beijing Summit of the Forum on China-Africa Cooperation(FOCAC),held in September 2024,elevated bilateral ties between China and African countries with diplomatic relations with China to a strategic level.It also redefined China-Africa relations as an“all-weather China-Africa community with a shared future for the new era.”This signifies that China and Africa will remain steadfast friends and close development partners,regardless of changes in the international landscape.展开更多
文摘Intervertebral disc （IVD） degeneration is the leading cause of disability with no disease-modifying treatment. IVD degeneration is associated with instable mechanical loading in the spine, but little is known about how mechanical stress regulates nucleus notochordal （NC） cells to maintain IVD homeostasis. Here we report that mechanical stress can result in excessive integrin αvβ6-mediated activation of transforming growth factor beta （TGFβ）, decreased NC cell vacuoles, and increased matrix proteoglycan production, and results in degenerative disc disease （DDD）. Knockout of TGFβ type II receptor （TβRII） or integrin αv in the NC cells inhibited functional activity of postnatal NC cells and also resulted in DDD under mechanical loading. Administration of RGD peptide, TGFβ, and αvβ6-neutralizing antibodies attenuated IVD degeneration. Thus, integrin-mediated activation of TGFβ plays a critical role in mechanical signaling transduction to regulate IVD cell function and homeostasis. Manipulation of this signaling pathway may be a potential therapeutic target to modify DDD.
基金The Science Fund of Health Bureau of Tianjin,No.2013KZ046
文摘Extensive lesions involving the posterior quadrant of the cerebral hemisphere （temporal, parietal, and occipital lobes） induce intractable epilepsy. These patients are potential candidates for surgical treatmenttu. Maintenance of isolated nerve tissue activity after surgery plays a crucial role in the neuroprotective effects of neurosurgery treatment. Disconnection surgery of the posterior quadrant is used to completely isolate nerve fibers, while blood supply at the isolated lobes is maintained. Subsequently, cavities caused by cystic or necrotic nerve tissues should be reduced as much as possible,
基金provided by Funding of State Key Laboratory of Oral Disease (Sichuan University, SKLOD201702)the National Science Foundation for Excellent Young Scholars of China (81322013)+1 种基金the Innovation Team of Sichuan Province (2015TD0011)Start-up Funding from State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, China (To Peng Liu)
文摘The adaptor protein NUMB is involved in asymmetric division and cell fate determination and recognized as an antagonist of Notch.Previous studies have proved that Notch activation in osteoblasts contributes to a high bone mass. In this study, however, an osteopenic phenotype was found in 9-week-old mice using osteoblastic specific Col1a1–2.3-Cre to ablate both Numb and its homologue Numbl. The trabecular bone mass decreased dramatically while the cortical bone mass was unaffected. Here, the Notch signal was not activated,while the tensin homologue deleted on human chromosome 10(PTEN), which dephosphorylates phosphatidylinositide 3-kinases, was elevated, attenuating protein kinase B(Akt). The ubiquitination assay revealed that NUMB may physiologically promote PTEN ubiquitination in the presence of neural precursor cell-expressed developmentally downregulated protein 4–1. In addition, the deficiency of Numb/Numbl also activated the Hedgehog pathway through GLI1. This process was found to improve the ratio of the receptor activator of nuclear factor-k B ligand to osteoprotegerin, which enhanced the differentiation of osteoclasts and bone resorption. In conclusion, this study provides an insight into new functons of NUMB and NUMBL on bone homeostasis.
文摘On August 2,the IEA,s World Energy Investment 2018 was issued in Beijing.Global energy investments totalled USD1.8 trillion in 2017,the third consecutive year of decline,and were 2% lower than those in 2016 after taking out the price factor.The declines in investment in the power generation sector were most significant,with declines in coal power,hydropower and nuclear power outpacing the increases in investment in photovoltaic power generation.
基金supported by the National Natural Science Foundation of China(Nos.82100977,82170964,81873716,and 82001042)China Postdoctoral Science Foundation(2021M691930)+7 种基金Shandong Province Key Research and Development Program(No.2021ZDSYS18)Shandong Province Major Scientific and Technical Innovation Project(No.2021SFGC0502)the Youth Scientific Research Funds of School of Stomatology,Shandong University(2020QNJJ03)the Opening Research Fund from Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University(2022LHM-KFKT004)the Construction Engineering Special Fund of“Taishan Scholars”of Shandong Province(No.ts20190975 and tsqn201909180)Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis,Therapy in Universities of Shandong,Rongxiang Regenerative Medicine Foundation of Shandong University(No.2019SDRX-14,2019SDRX-15)the Fundamental Research Funds of Shandong UniversityQilu Young Scholar Foundation of Shandong University。
文摘Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects may yield suboptimal effects due to the complexity of the periodontal inflammatory environment. Herein, stem cell spheroid is encapsulated by interfacial assembly of metal-phenolic network(MPN) nanofilm to form a stem cell microsphere capsule. Specifically, periodontal ligament stem cells(PDLSCs) spheroid was coated with FeⅢ/tannic acid coordination network to obtain spheroid@[FeⅢ-TA] microcapsules. The formed biodegradable MPN biointerface acted as a cytoprotective barrier and exhibited antioxidative, antibacterial and anti-inflammatory activities, effectively remodeling the inflammatory microenvironment and maintaining the stemness of PDLSCs. The stem cell microencapsulation proposed in this study can be applied to multiple stem cells with various functional metal ion/polyphenol coordination, providing a simple yet efficient delivery strategy for stem cell stemness maintenance in an inflammatory environment toward a better therapeutic outcome.
基金supported by the National Key Research and Development Program of China(2018YFD1000800)a grant from the National Natural Science Foundation of China(31722006)the Key Research Program of Fujian Province(2018NZ0002)to S.W.
文摘Flooding tolerance is an important trait for tomato breeding.In this study,we obtained a recessive mutant exhibiting highly enhanced submergence resistance.Phenotypical analyses showed that this resistant to flooding(rf)mutant displays slightly chlorotic leaves and spontaneous initiation of adventitious roots(ARs)on stems.The mutation was mapped to the phytochromobilin synthase gene AUREA(AU),in which a single amino acid substitution from asparagine to tyrosine occurred.In addition to the classic function of AU in phytochrome and chlorophyll biogenesis in leaves,we uncovered its novel role in mediating AR formation on stems.We further observed temporal coincidence of the two phenotypes in the rf mutant:chlorosis and spontaneous AR formation and revealed that AU functions by maintaining heme homeostasis.Interestingly,our grafting results suggest that heme might play roles in AR initiation via long-distance transport from leaves to stems.Our results present genetic evidence for the involvement of the AU–heme oxygenase-1–heme pathway in AR initiation in tomato.As fruit production and yield in the rf mutant are minimally impacted,the mutation identified in this study may provide a target for biotechnological renovation of tomato germplasm in future breeding.
文摘"IDC predicts that in 2014,the healthcare informatization will continue to maintain high-speed development,mobile health and clinic data center will become the two hot spots of informatization construction of hospitals,and the clinic decision-supporting system will be applied on trial.The policy of multi-sited license of physicians will enable the RHIS(Regional Healthcare Informatization System)to start another construction upsurge,and
文摘From December 2 to 4,2022,Guangzhou hosted the 19th International Finance Forum(IFF)annual meeting,themed“The Ever-Changing World:Reshaping Our Shared Future.”The event was attended by leaders of world organizations including the United Nations,the International Monetary Fund,and the World Bank.
基金funded by the National Natural Science Foundation of China (Grant No.31730082).
文摘Normal plant growth and development ensures the timely maturity and expected yield of crops.The repair mechanism of genome damage under adverse circumstances is essential for maintaining regular plant growth.Herein,two allelic growth retardation mutants of Chinese cabbage,grm1 and grm2,were obtained from EMS-mutagenized populations of wild-type'FT'isolated-cultured microspores and seeds,respectively.Both mutants stably inherited and exhibited stunted growth with smaller leafy-heads.Genetic analysis and allelism test manifested that the mutated trait was triggered by a same single recessive nuclear gene.Via BSR-Seq,Brgrm1 was mapped to a target region including 20 genes on chromosome A09.Whole-genome re-sequencing revealed that BraA09g024830.3C in grm1 had a single base(A)deletion in the 17th exon,leading to a termination codon(TAG).Genotyping showed that the mutated phenotype co-segregated with the InDel in recombinants of the closest linkage markers.In addition,cloning of BraA09g024830.3C in grm2 found that a base substitution(G-A)occurred in the last base of the 1st intron causing an additional 263-bp retention in coding sequences,which in turn led to a termination codon(TAA).BraA09g024830.3C(BrDDB1A)is a homolog of Arabidopsis thaliana Damaged DNA Binding Protein 1A(DDB1A),a key gene of UV tolerance involved in DNA damage repair.The two mutants exhibited normal plant growth identical with wild-type under an extremely low UV radiation.Our results demonstrated that BrDDB1A contributes to maintaining regular plant growth in Chinese cabbage,which provide insights into elucidating the molecular mechanism underlying Chinese cabbage growth and development.
基金the National Natural Science Foundation of China(82372412)the Social Development Project of Jiangsu Province(BE2022701)+4 种基金the Top Talent Support Program for Young and Middle-aged People of the Wuxi Health Committee(BJ2020044,BJ2020057,HB2020043)the Fundamental Research Funds of the Health and Family Planning Commission of Wuxi(M202024)the Special Program for Translational Medicine Research of the Wuxi Translational Medicine Center(2020DHYB07,2020DHYB03)the Key Special Project of Precision Medicine of the Wuxi Health Commission(J202101)peking union medical college hospital talent cultivation program(UHB50192).
文摘Rheumatoid arthritis(RA)is a systemic autoimmune disease in which synovial fibroblasts(SFs)maintain chronic inflammation by secreting proinflammatory mediators,leading to joint destruction.While the role of proinflammatory mediators in this process is well-established,the contribution of non-inflammatory regulators in SFs to joint pathology remains poorly understood.In this study,we investigated the non-inflammatory role of SFs in RA using a co-culture model,and found that SFs from RA patients promote apoptosis of human chondrocytes.Mechanistic investigations reveal that SFs can secrete small extracellular vesicles(sEVs),which are taken up by chondrocytes and induce chondrocyte apoptosis in both normal chondrocytes and chondrocytes from patients with RA.sEV-derived miRNA 15-29148 are identified as key signaling molecules mediating the apoptosis effects of chondrocytes.Further studies reveal that SF-derived miRNA 15-29148 targeting CIAPIN1 results in increased chondrocyte apoptosis.We further demonstrate that SF-derived miRNA 15-29148 is transferred to chondrocytes,exacerbating cartilage damage in vivo.Moreover,chondrocyte-specific aptamer-modified polyamidoamine nanoparticles not only ameliorated RA but also prevented its onset.This study suggests that,in RA,the secretion of specific sEV-miRNAs from SFs plays a crucial role in promoting chondrocyte apoptosis,potentially through non-inflammatory regulation,and that sEV-miRNA inhibition in SFs may represent an early preventive treatment strategy for cartilage degradation in RA.
基金supported by funds from the Italian Ministry of Health,Ricerca Finalizzata,(Grant N.GR-2013-02355882 and GR-2021-12373946 to AL)5x1000 Project of the Istituto Superiore di Sanità(Project code:ISS5x1000_21-949432e8c9be to AL)the European Union–NextGeneration EU through the Italian Ministry of University and Research under PNRR-M4C2-I1.3 Project PE_00000019“HEAL ITALIA”to EA(CUP I83C22001830006)。
文摘Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental stage to adult life.To maintain the optimal functionality of the brain,astroglial cells are particularly committed to reacting to every change in tissue homeostatic conditions,from mild modifications of the physiological environment,a process called astrocyte activation,to the more severe alterations occurring in pathological situations causing astrocyte reactivity or reactive astrogliosis(Escartin et al.,2021).During these reactive states,astrocytes mount an active,progressive response encompassing morphological,molecular,and interactional remodeling,leading to the acquisition of new functions and the loss of others,whose intensity,duration,and reversibility are dependent on the nature of the stimulus and regulated in a context-specific manner.
基金supported by core funding provided by Temasek Life Sciences Laboratory(to CTO)。
文摘Apolipoprotein E has diverse functions in neurons:Apolipoprotein E(ApoE)is a glycoprotein that primarily regulates lipid metabolism and transport in the central nervous system.There are three predominant human ApoE protein isoforms with cysteine and arginine substitutions at amino acid positions 112 and 158 that impact their lipidation and related functions(Flowers and Rebeck,2020).ApoE2 is characterized by Cys112 and Cys158,ApoE3 by Cys112 and Arg158,whereas ApoE4 contains Arg112 and Arg158.
文摘As 2024 comes to an end,the pickup in consumption and robust growth in a range of sectors have bolstered confidence that the Chinese economy is right on track to meet the preset growth rate of around five percent.Benefiting from a flurry of consumption-stimulating policies and measures,China’s online retail sales surpassed RMB 14 trillion in the January-November period,a year-on-year increase of 7.4 percent.Imports and exports maintained steady growth,climbing by 4.9 percent year-on-year in renminbi terms.Value-added industrial output also expanded 5.8 percent year-on-year.The volume of express delivery parcels reached a new high of 150 billion and is expected to grow higher still given the new-year festivities ahead.
文摘China’s foreign trade registered steady growthinthefirstfivemonthsof theyeardespitegrowingexternal uncertainties.Thecountry’sforeigntradeofgoods rose 4.1 percent year on year during that time to 12.1 trillion yuan ($1.75 trillion), data from the General Administration of Customs of China (GACC) showed on June 10.
文摘In life,we are bound to encounter various difficulties and setbacks.However,it is of great significance to maintain a positive attitude when were facing these challenges.For me,this lesson was deeply impressed upon me during a particularly tough period.
文摘If you are to choose an event that can best represent China’s culture,undoubtedly,it will be the Spring Festival,or the Chinese Lunar New Year.It is a cherished occasion for families to get together,extend best wishes to the people around them,and maintain friendships.
文摘China’s economy is expected to remain stable throughout 2025.According to projections by the Center for Forecasting Science of the Academy of Mathematics and Systems Science under the Chinese Academy of Sciences,China’s GDP(gross domestic product)growth should settle at around 4.8 percent this year and will likely hit 5 percent if the government’s stimulus package is effectively implemented.I personally believe that the Chinese economy will maintain growth of about 4.6 percent in 2025 with robust performance in the first and fourth quarters and slower economic activity in the second and third quarters.
文摘The annual Central Economic Work Conference 2024,held on December 11-12,set the overall goal and policy framework for Chinese economy in 2025,a crucial year in China’s economic development as the final year of the 14th Five-Year Plan(FYP)and the preparation for the 15th FYP.A series of incremental fiscal and monetary policies adopted since the end of September has seen their initial effects in stabilizing stock markets and boosting consumption and production and thus enhanced the confidence of meeting the GDP goal for 2024.In the face of complicated external challenges and domestic difficulties-inadequate effective demand,overcapacity in some sectors and weak expectations,it is crucial to maintain stable growth in 2025.The Central Economic Work Conference 2024 set the general goal of 2025 on maintaining stable growth,employment and consumer prices.
文摘China-Africa relations maintained their upward trajectory in 2024,with new plans for future cooperation taking shape.Chinese President Xi Jinping described the China-Africa partnership as being at its best in history.The Beijing Summit of the Forum on China-Africa Cooperation(FOCAC),held in September 2024,elevated bilateral ties between China and African countries with diplomatic relations with China to a strategic level.It also redefined China-Africa relations as an“all-weather China-Africa community with a shared future for the new era.”This signifies that China and Africa will remain steadfast friends and close development partners,regardless of changes in the international landscape.
