Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared us...Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared using static wet chemical technique,but these face risks of delamination or deactivation induced by blood flow.Inspired by the flow-shear-stress mediated conformation changes of von Willebrand factor,a novel fluid-driven deposition technique was employed to apply polydopamine-heparin coatings within CBPs.Moreover,most FDA-approved CBPs are designed for high-flow-rate CBPs of major organs like the heart and lungs(1000~8000 ml/min).Few are tailored for low-flow-rate perfusion of other organs such as the liver,kidney and brain(<50-300 mL/min).Our approach addresses this gap by developing low-flow-rate CBPs through anti-thrombogenic coat-ings and anti-hemolytic structural optimizations.In this study,we introduced an axial magnetic direct drive motor with our optimized low-flow-rate CBPs,achieving a stable-low-flow-rate rate ranging from 16.3 mL/min(300 rpm)to 121.0 mL/min(2000 rpm).The resulting CBPs system exhibited enhanced flow stability and hemocompatibility in rabbit model experiments,demonstrating significantly lower hemolysis rates and lower thrombus formation risks.These results indicate that the polydopamine-assisted heparin coating provides shortterm stability under dynamic flow,offering a promising strategy for low-flow-rate CBPs,though its long-term durability and clinical translation potential require further validation.展开更多
基金Beijing Natural Science Foundation 7244510(MJ)CHF-brain 2024-1-5041(MJ)+7 种基金CHF-brain 2024-3-2062(ML)Collaborative innovation project by Chinese Institutes for Medical Research CX25XT02(MJ)National Natural Science Foundation of China 82402444(MJ),52531008(ML),82102220(ML),82027802(XJ)Beijing Hospitals Authority Clinical Medicine Development of special funding support(YGLX202527)(MJ)Excellent Youth Fund of Capital Medical University B2305(ML)The Non-profit Central Research Institute Fund of Chinese Academy of Medical 2023-JKCS-09(ML),2023-JKCS-13(MJ)Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support from Yangfan Project YGLX202325(ML)Research Funding on Translational Medicine from Beijing Municipal Science and Technology Commission Z221100007422023(ML)。
文摘Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared using static wet chemical technique,but these face risks of delamination or deactivation induced by blood flow.Inspired by the flow-shear-stress mediated conformation changes of von Willebrand factor,a novel fluid-driven deposition technique was employed to apply polydopamine-heparin coatings within CBPs.Moreover,most FDA-approved CBPs are designed for high-flow-rate CBPs of major organs like the heart and lungs(1000~8000 ml/min).Few are tailored for low-flow-rate perfusion of other organs such as the liver,kidney and brain(<50-300 mL/min).Our approach addresses this gap by developing low-flow-rate CBPs through anti-thrombogenic coat-ings and anti-hemolytic structural optimizations.In this study,we introduced an axial magnetic direct drive motor with our optimized low-flow-rate CBPs,achieving a stable-low-flow-rate rate ranging from 16.3 mL/min(300 rpm)to 121.0 mL/min(2000 rpm).The resulting CBPs system exhibited enhanced flow stability and hemocompatibility in rabbit model experiments,demonstrating significantly lower hemolysis rates and lower thrombus formation risks.These results indicate that the polydopamine-assisted heparin coating provides shortterm stability under dynamic flow,offering a promising strategy for low-flow-rate CBPs,though its long-term durability and clinical translation potential require further validation.
