Magnesium-lithium hybrid batteries(MLHBs)have gained increasing attention due to their combined advantages of rapid ion insertion/extraction cathode and magnesium metal anode.Herein,Sn S_(2)-SPAN hybrid cathode with s...Magnesium-lithium hybrid batteries(MLHBs)have gained increasing attention due to their combined advantages of rapid ion insertion/extraction cathode and magnesium metal anode.Herein,Sn S_(2)-SPAN hybrid cathode with strong C-Sn bond and rich defects is ingeniously constructed to realize Mg^(2+)/Li^(+)co-intercalation.The physical and chemical double-confinement synergistic engineering of sulfurized polyacrylonitrile can suppress the agglomeration of Sn S_(2)nanoparticles and the volume expansion,simultaneously promote charge transfer and enhance structural stability.The introduced abundant sulfur vacancies provide more active sites for Mg^(2+)/Li^(+)co-intercalation.Meanwhile,the beneficial effects of rich sulfur defects and C-Sn bond on enhanced electrochemical properties are further evidenced by density-functional theory(DFT)calculations.Therefore,compared with pristine SnS_(2),SnS_(2)-SPAN cathode displays high specific capacity(218 m Ah g^(-1)at 0.5A g^(-1)over 700 cycles)and ultra-long cycling life(101 m Ah g^(-1)at 5 A g^(-1)up to 28,000 cycles).And a high energy density of 307 Wh kg^(-1)can be realized by the Sn S_(2)-SPAN//Mg pouch cell.Such elaborate and simple design supplies a reference for the exploitation of advanced cathode materials with excellent electrochemical properties for MLHBs.展开更多
Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in ...Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in bone reconstruction.We review the history of MBs and their excellent biocompatibility,biodegradability and osteopromotive properties,highlighting them as candidates for a new generation of biodegradable orthopedic implants.In particular,the results reported in the field-specific literature(280 articles)in recent decades are dissected with respect to the extensive variety of MBs for orthopedic applications,including Mg/Mg alloys,bioglasses,bioceramics,and polymer materials.We also summarize the osteogenic mechanism of MBs,including a detailed section on the physiological process,namely,the enhanced osteogenesis,promotion of osteoblast adhesion and motility,immunomodulation,and enhanced angiogenesis.Moreover,the merits and limitations of current bone grafts and substitutes are compared.The objective of this review is to reveal the strong potential of MBs for their use as agents in bone repair and regeneration and to highlight issues that impede their clinical translation.Finally,the development and challenges of MBs for transplanted orthopedic materials are discussed.展开更多
The addition of nanoscale additions to magnesium(Mg)based alloys can boost mechanical characteristics without noticeably decreasing ductility.Since Mg is the lightest structural material,the Mg-based nanocomposites(NC...The addition of nanoscale additions to magnesium(Mg)based alloys can boost mechanical characteristics without noticeably decreasing ductility.Since Mg is the lightest structural material,the Mg-based nanocomposites(NCs)with improved mechanical properties are appealing materials for lightweight structural applications.In contrast to conventional Mg-based composites,the incorporation of nano-sized reinforcing particles noticeably boosts the strength of Mg-based nanocomposites without significantly reducing the formability.The present article reviews Mg-based metal matrix nanocomposites(MMNCs)with metallic and ceramic additions,fabricated via both solid-based(sintering and powder metallurgy)and liquid-based(disintegrated melt deposition)technologies.It also reviews strengthening models and mechanisms that have been proposed to explain the improved mechanical characteristics of Mg-based alloys and nanocomposites.Further,synergistic strengthening mecha-nisms in Mg matrix nanocomposites and the dominant equations for quantitatively predicting mechanical properties are provided.Furthermore,this study offers an overview of the creep and fatigue behavior of Mg-based alloys and nanocomposites using both traditional(uniaxial)and depth-sensing indentation techniques.The potential applications of magnesium-based alloys and nanocomposites are also surveyed.展开更多
Biodegradable metals such as magnesium(Mg)and its alloys have attracted extensive attention in biomedical research due to their excellent mechanical properties and biodegradability.However,traditional casting,extrusio...Biodegradable metals such as magnesium(Mg)and its alloys have attracted extensive attention in biomedical research due to their excellent mechanical properties and biodegradability.However,traditional casting,extrusion,and commercial processing have limitations in manufacturing components with a complex shape/structure,and these processes may produce defects such as cavities and gas pores which can degrade the properties and usefulness of the products.Compared to conventional techniques,additive manufacturing(AM)can be used to precisely control the geometry of workpieces made of different Mg-based materials with multiple geometric scales and produce desirable medical products for orthopedics,dentistry,and other fields.However,a detailed and thorough understanding of the raw materials,manufacturing processes,properties,and applications is required to foster the production of commercial Mg-based biomedical components by AM.This review summarizes recent advances and important issues pertaining to AM of Mg-based biomedical products and discusses future development and application trends.展开更多
Magnesium-based biomaterials(MBMs)are one of the most promising materials for tissue engineering due to their unique mechanical properties and excellent functional properties.This review describes the development,adva...Magnesium-based biomaterials(MBMs)are one of the most promising materials for tissue engineering due to their unique mechanical properties and excellent functional properties.This review describes the development,advantages,and challenges of MBMs for biomedical applications,especially for tissue repair and regeneration.The history of the use of MBMs from the beginning of the 20th century is traced,and the transformative advances in contemporary applications of MBMs in areas such as orthopedics and cardiovascular surgery are emphasized.The review also provides insight into the signaling pathways affected by MBMs,such as the PI3K/Akt and RANKL/RANK/OPG pathways,which are critical for osteogenesis and angiogenesis.The review advocates that future research should focus on optimizing alloy compositions,surface modification and exploring innovative technologies such as 3D printing to improve the efficacy of MBMs in complex tissue repair.The potential of MBMs to tissue engineering and regenerative medicine is significant,urging further exploration and interdisciplinary collaboration to maximize their therapeutic effects.展开更多
We investigate experimentally and analytically the combustion behavior of a high-metal magnesium-based hydro- reactive fuel under high temperature gaseous atmosphere. The fuel studied in this paper contains 73% magnes...We investigate experimentally and analytically the combustion behavior of a high-metal magnesium-based hydro- reactive fuel under high temperature gaseous atmosphere. The fuel studied in this paper contains 73% magnesium powders. An experimental system is designed and experiments are carried out in both argon and water vapor atmo- spheres. It is found that the burning surface temperature of the fuel is higher in water vapor than that in argon and both of them are higher than the melting point of magnesium, which indicates the molten state of magnesium particles in the burning surface of the fuel. Based on physical considerations and experimental results, a mathematical one-dimensional model is formulated to describe the combustion behavior of the high-metal magnesium-based hydro-reactive fuel. The model enables the evaluation of the burning surface temperature, the burning rate and the flame standoff distance each as a function of chamber pressure and water vapor concentration. The results predicted by the model show that the burning rate and the surface temperature increase when the chamber pressure and the water vapor concentration increase, which are in agreement with the observed experimental trends.展开更多
This study explores the potential of Mg/Carbon Nanotubes/Baghdadite composites as biomaterials for bone regeneration and repair while addressing the obstacles to their clinical application.BAG powder was synthesized u...This study explores the potential of Mg/Carbon Nanotubes/Baghdadite composites as biomaterials for bone regeneration and repair while addressing the obstacles to their clinical application.BAG powder was synthesized using the sol-gel method to ensure a fine distribution within the Mg/CNTs matrix.Mg/1.5 wt.%CNT composites were reinforced with BAG at weight fractions of 0.5,1.0,and 1.5 wt.%using spark plasma sintering at 450℃and 50 MPa after homogenization via ball milling.The cellular bioactivity of these nanocomposites was evaluated using human osteoblast-like cells and adipose-derived mesenchymal stromal cells.The proliferation and attachment of MG-63cells were assessed and visualized using the methylthiazol tetrazolium(MTT)assay and SEM,while AD-MSC differentiation was measured using alkaline phosphatase activity assays.Histograms were also generated to visualize the diameter distributions of particles in SEM images using image processing techniques.The Mg/CNTs/0.5 wt.%BAG composite demonstrated optimal mechanical properties,with compressive strength,yield strength,and fracture strain of 259.75 MPa,180.25 MPa,and 31.65%,respectively.Machine learning models,including CNN,LSTM,and GRU,were employed to predict stress-strain relationships across varying BAG amounts,aiming to accurately model these curves without requiring extensive physical experiments.As shown by contact angle measurements,enhanced hydrophilicity promoted better cell adhesion and proliferation.Furthermore,corrosion resistance improved with a higher BAG content.This study concludes that Mg/CNTs composites reinforced with BAG concentrations below 1.0 wt.%offer promising biodegradable implant materials for orthopedic applications,featuring adequate load-bearing capacity and improved corrosion resistance.展开更多
Mg-based materials have potential applications in the field of orthopedics owing to their good biodegradability,biocompatibility,and boneinducing properties.However,during the early application process,their major dra...Mg-based materials have potential applications in the field of orthopedics owing to their good biodegradability,biocompatibility,and boneinducing properties.However,during the early application process,their major drawback was rapid degradation rate,which limited their clinical application.Nanoparticles can effectively reinforce the mechanical strength and corrosion resistance of Mg matrices,and different nanoparticles can be selected to achieve different biological functions.Therefore,Mg-based nanocomposites have emerged as a versatile class of degradable implant materials with broad clinical potential.This review summarizes the research progress of Mg-based orthopedic implants,mainly including the reinforcement mechanism of nanoparticles on Mg-based materials,the effects and biological functions of different nanoparticle enhancers,surface modification,and the application of new manufacturing technologies.Furthermore,the degradation process of Mg-based materials and the biological functions of magnesium ion(Mg^(2+))during the degradation process are discussed in detail We focused on the biological mechanisms through which Mg^(2+)promotes bone and vascular formation and inhibits osteoclasts by regulating the immune microenvironment or multiple signaling pathways.Finally,the clinical application of Mg-based orthopedic implants are introduced and the future research directions of Mg-based nanocomposites are discussed.展开更多
Magnesium(Mg)and its alloys are revolutionizing the field of interventional surgeries in the medical industry.Their high biocompatibility,biodegradability,and a similar elastic modulus to natural bone make porous Mg-b...Magnesium(Mg)and its alloys are revolutionizing the field of interventional surgeries in the medical industry.Their high biocompatibility,biodegradability,and a similar elastic modulus to natural bone make porous Mg-based structures potential candidates for orthopedic implants and tissue engineering scaffolding.However,fabricating and machining porous Mg-based structures is challenging due to their complexity and difficulties in achieving uniform or gradient porosity.This review aims to thoroughly explore various fabrication procedures used to create metallic scaffolds,with a specific focus on those made from Mg-based alloys.Both traditional manufacturing techniques,including the directional solidification of metal-gas eutectic technique,pattern casting,methods using space holders,and modern fabrication methods,which are based on additive manufacturing,are covered in this review article.Furthermore,the paper highlights the most important findings of recent studies on Mg-based scaffolds in terms of their microstructure specifications,mechanical properties,degradation and corrosion behavior,antibacterial activity,and biocompatibility(both in vivo and in vitro).While extensive research has been conducted to optimize manufacturing parameters and qualities of Mg-based scaffolds for use in biomedical applications,specifically for bone tissue engineering applications,further investigation is needed to fabricate these scaffolds with specific properties,such as high resistance to corrosion,good antibacterial properties,osteoconductivity,osteoinductivity,and the ability to elicit a favorable response from osteoblast-like cell lines.The review concludes with recommendations for future research in the field of medical applications.展开更多
Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic prop-erties.However,conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5...Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic prop-erties.However,conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts in vivo outcomes.In this study,we aimed to establish an in vitro evaluation model that better correlates with in vivo performance.Three-layer BMSC cell sheets were constructed using layer-by-layer assembly.Histological analyses revealed a stable three-dimensional structure with elevated cell-cell interaction proteins,including N-Cadherin,Fibronectin,and Vinculin,along with enhanced osteogenic potential.The cytotoxicity of 4N pure Mg was evaluated in both cell sheet and monolayer co-culture models.Flow cytometry showed higher Ki67 expression and lower ROS levels and apoptosis rate in cell sheets.ShRNA-mediated silencing of N-Cadherin in cell sheets significantly compromised their cytopro-tective capacity against Mg metal-induced toxicity.Osteogenesis-related gene expression correlation analysis between in vitro co-culture models and in vivo femur implantation models was conducted using RNA-seq and qRT-PCR.Results showed that 4N pure Mg enhanced osteogenic genes(BMP2R,RUNX2,and SP7)in cell sheets,consistent with in vivo patterns but contrary to monolayer models.Various Mg-based metals(4N/5N Pure Mg,ZE21B,and WE43)were evaluated in cell sheet defect,monolayer defect,and cranial defect models.5N Pure Mg,ZE21B,and WE43 promoted defect healing in both cranial defect and cell sheets,but showed no positive effect in monolayers.Collectively,cell sheet models correlated well with in vivo results,suggesting their potential as alternative in vitro evaluation models,thereby accelerating clinical translation of Mg-based biomaterials.展开更多
基金financially supported by the National Key R&D Program of China(No.2023YFB3809500)National Natural Science Foundation of China(Grant No.51931006,52272240 and U22A20118)+2 种基金the Fundamental Research Funds for the Central Universities of China(Xiamen University:No.20720220074)Science and Technology Projects of Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(HRTP-[2022]-22)the“Double-First Class”Foundation of Materials Intelligent Manufacturing Discipline of Xiamen University。
文摘Magnesium-lithium hybrid batteries(MLHBs)have gained increasing attention due to their combined advantages of rapid ion insertion/extraction cathode and magnesium metal anode.Herein,Sn S_(2)-SPAN hybrid cathode with strong C-Sn bond and rich defects is ingeniously constructed to realize Mg^(2+)/Li^(+)co-intercalation.The physical and chemical double-confinement synergistic engineering of sulfurized polyacrylonitrile can suppress the agglomeration of Sn S_(2)nanoparticles and the volume expansion,simultaneously promote charge transfer and enhance structural stability.The introduced abundant sulfur vacancies provide more active sites for Mg^(2+)/Li^(+)co-intercalation.Meanwhile,the beneficial effects of rich sulfur defects and C-Sn bond on enhanced electrochemical properties are further evidenced by density-functional theory(DFT)calculations.Therefore,compared with pristine SnS_(2),SnS_(2)-SPAN cathode displays high specific capacity(218 m Ah g^(-1)at 0.5A g^(-1)over 700 cycles)and ultra-long cycling life(101 m Ah g^(-1)at 5 A g^(-1)up to 28,000 cycles).And a high energy density of 307 Wh kg^(-1)can be realized by the Sn S_(2)-SPAN//Mg pouch cell.Such elaborate and simple design supplies a reference for the exploitation of advanced cathode materials with excellent electrochemical properties for MLHBs.
基金financial support from the National Natural Science Foundation of China(No.81672230)the Natural Science Foundation of Chongqing(No.cstc2020jcyjmsxm2234)+1 种基金the Top-notch Young Talent Project of Chongqing Traditional Chinese Medicine Hospital(No.CQSZYY2020008)the Chongqing Graduate Research Innovation Project(No.CYS20199)。
文摘Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in bone reconstruction.We review the history of MBs and their excellent biocompatibility,biodegradability and osteopromotive properties,highlighting them as candidates for a new generation of biodegradable orthopedic implants.In particular,the results reported in the field-specific literature(280 articles)in recent decades are dissected with respect to the extensive variety of MBs for orthopedic applications,including Mg/Mg alloys,bioglasses,bioceramics,and polymer materials.We also summarize the osteogenic mechanism of MBs,including a detailed section on the physiological process,namely,the enhanced osteogenesis,promotion of osteoblast adhesion and motility,immunomodulation,and enhanced angiogenesis.Moreover,the merits and limitations of current bone grafts and substitutes are compared.The objective of this review is to reveal the strong potential of MBs for their use as agents in bone repair and regeneration and to highlight issues that impede their clinical translation.Finally,the development and challenges of MBs for transplanted orthopedic materials are discussed.
基金H.R.Bakhsheshi-Rad and S.Sharif would like to acknowledge UTM Research Management for the financial support through the funding(Q.J130000.2409.08G37).
文摘The addition of nanoscale additions to magnesium(Mg)based alloys can boost mechanical characteristics without noticeably decreasing ductility.Since Mg is the lightest structural material,the Mg-based nanocomposites(NCs)with improved mechanical properties are appealing materials for lightweight structural applications.In contrast to conventional Mg-based composites,the incorporation of nano-sized reinforcing particles noticeably boosts the strength of Mg-based nanocomposites without significantly reducing the formability.The present article reviews Mg-based metal matrix nanocomposites(MMNCs)with metallic and ceramic additions,fabricated via both solid-based(sintering and powder metallurgy)and liquid-based(disintegrated melt deposition)technologies.It also reviews strengthening models and mechanisms that have been proposed to explain the improved mechanical characteristics of Mg-based alloys and nanocomposites.Further,synergistic strengthening mecha-nisms in Mg matrix nanocomposites and the dominant equations for quantitatively predicting mechanical properties are provided.Furthermore,this study offers an overview of the creep and fatigue behavior of Mg-based alloys and nanocomposites using both traditional(uniaxial)and depth-sensing indentation techniques.The potential applications of magnesium-based alloys and nanocomposites are also surveyed.
基金This work was financially supported by the Guangdong Basic and Applied Basic Research Foundation(No.2020B1515120078,2021A1515111140,and 2021B1515120059)National Key Research and Development Project of China(No.2020YFC1107202)+3 种基金Science Research Cultivation Program(PY2022002)Science and Technology Planning Project of Guangzhou(No.202206010030)City University of Hong Kong Donation Research Grants[DONRMG No.9229021 and 9220061]as well as City University of Hong Kong Strategic Research Grant[SRG 7005505].
文摘Biodegradable metals such as magnesium(Mg)and its alloys have attracted extensive attention in biomedical research due to their excellent mechanical properties and biodegradability.However,traditional casting,extrusion,and commercial processing have limitations in manufacturing components with a complex shape/structure,and these processes may produce defects such as cavities and gas pores which can degrade the properties and usefulness of the products.Compared to conventional techniques,additive manufacturing(AM)can be used to precisely control the geometry of workpieces made of different Mg-based materials with multiple geometric scales and produce desirable medical products for orthopedics,dentistry,and other fields.However,a detailed and thorough understanding of the raw materials,manufacturing processes,properties,and applications is required to foster the production of commercial Mg-based biomedical components by AM.This review summarizes recent advances and important issues pertaining to AM of Mg-based biomedical products and discusses future development and application trends.
基金supported by the National Natural Science Foundation of China(Grant No.82202672)the Key Research and Development Program of Anhui Province(No.2022e07020017)+5 种基金China Postdoctoral Science Foundation Grant(2022M723049)the National Postdoctoral Program for Innovative Talents(BX20230350)Research Funds of Centre for Leading Medicine and Advanced Technologies of IHM(No.2023IHM02007)the Foundation of National Center for Translational Medicine(Shanghai)SHU Branch(No.SUITM-202301)Anhui Provincial Research Preparation Plan(2022AH040074),Natural science fund for colleges and universities in Anhui Proxince(2023AH053289)Research Fund of Anhui Institute of translational medicine(2022zhyx-C32).
文摘Magnesium-based biomaterials(MBMs)are one of the most promising materials for tissue engineering due to their unique mechanical properties and excellent functional properties.This review describes the development,advantages,and challenges of MBMs for biomedical applications,especially for tissue repair and regeneration.The history of the use of MBMs from the beginning of the 20th century is traced,and the transformative advances in contemporary applications of MBMs in areas such as orthopedics and cardiovascular surgery are emphasized.The review also provides insight into the signaling pathways affected by MBMs,such as the PI3K/Akt and RANKL/RANK/OPG pathways,which are critical for osteogenesis and angiogenesis.The review advocates that future research should focus on optimizing alloy compositions,surface modification and exploring innovative technologies such as 3D printing to improve the efficacy of MBMs in complex tissue repair.The potential of MBMs to tissue engineering and regenerative medicine is significant,urging further exploration and interdisciplinary collaboration to maximize their therapeutic effects.
基金Project supported by the Young Scientist Fund of the National Natural Science Foundation of China(Grant No.51006118)
文摘We investigate experimentally and analytically the combustion behavior of a high-metal magnesium-based hydro- reactive fuel under high temperature gaseous atmosphere. The fuel studied in this paper contains 73% magnesium powders. An experimental system is designed and experiments are carried out in both argon and water vapor atmo- spheres. It is found that the burning surface temperature of the fuel is higher in water vapor than that in argon and both of them are higher than the melting point of magnesium, which indicates the molten state of magnesium particles in the burning surface of the fuel. Based on physical considerations and experimental results, a mathematical one-dimensional model is formulated to describe the combustion behavior of the high-metal magnesium-based hydro-reactive fuel. The model enables the evaluation of the burning surface temperature, the burning rate and the flame standoff distance each as a function of chamber pressure and water vapor concentration. The results predicted by the model show that the burning rate and the surface temperature increase when the chamber pressure and the water vapor concentration increase, which are in agreement with the observed experimental trends.
文摘This study explores the potential of Mg/Carbon Nanotubes/Baghdadite composites as biomaterials for bone regeneration and repair while addressing the obstacles to their clinical application.BAG powder was synthesized using the sol-gel method to ensure a fine distribution within the Mg/CNTs matrix.Mg/1.5 wt.%CNT composites were reinforced with BAG at weight fractions of 0.5,1.0,and 1.5 wt.%using spark plasma sintering at 450℃and 50 MPa after homogenization via ball milling.The cellular bioactivity of these nanocomposites was evaluated using human osteoblast-like cells and adipose-derived mesenchymal stromal cells.The proliferation and attachment of MG-63cells were assessed and visualized using the methylthiazol tetrazolium(MTT)assay and SEM,while AD-MSC differentiation was measured using alkaline phosphatase activity assays.Histograms were also generated to visualize the diameter distributions of particles in SEM images using image processing techniques.The Mg/CNTs/0.5 wt.%BAG composite demonstrated optimal mechanical properties,with compressive strength,yield strength,and fracture strain of 259.75 MPa,180.25 MPa,and 31.65%,respectively.Machine learning models,including CNN,LSTM,and GRU,were employed to predict stress-strain relationships across varying BAG amounts,aiming to accurately model these curves without requiring extensive physical experiments.As shown by contact angle measurements,enhanced hydrophilicity promoted better cell adhesion and proliferation.Furthermore,corrosion resistance improved with a higher BAG content.This study concludes that Mg/CNTs composites reinforced with BAG concentrations below 1.0 wt.%offer promising biodegradable implant materials for orthopedic applications,featuring adequate load-bearing capacity and improved corrosion resistance.
基金Scientific Development Program of Jilin Province(20220204103YY)。
文摘Mg-based materials have potential applications in the field of orthopedics owing to their good biodegradability,biocompatibility,and boneinducing properties.However,during the early application process,their major drawback was rapid degradation rate,which limited their clinical application.Nanoparticles can effectively reinforce the mechanical strength and corrosion resistance of Mg matrices,and different nanoparticles can be selected to achieve different biological functions.Therefore,Mg-based nanocomposites have emerged as a versatile class of degradable implant materials with broad clinical potential.This review summarizes the research progress of Mg-based orthopedic implants,mainly including the reinforcement mechanism of nanoparticles on Mg-based materials,the effects and biological functions of different nanoparticle enhancers,surface modification,and the application of new manufacturing technologies.Furthermore,the degradation process of Mg-based materials and the biological functions of magnesium ion(Mg^(2+))during the degradation process are discussed in detail We focused on the biological mechanisms through which Mg^(2+)promotes bone and vascular formation and inhibits osteoclasts by regulating the immune microenvironment or multiple signaling pathways.Finally,the clinical application of Mg-based orthopedic implants are introduced and the future research directions of Mg-based nanocomposites are discussed.
基金U.S.National Institute of Health-National Heart,Lung,and Blood Institute Grants R56HL163552 and R01HL168696 are acknowledged by J W D for funding work on biodegradable metals.
文摘Magnesium(Mg)and its alloys are revolutionizing the field of interventional surgeries in the medical industry.Their high biocompatibility,biodegradability,and a similar elastic modulus to natural bone make porous Mg-based structures potential candidates for orthopedic implants and tissue engineering scaffolding.However,fabricating and machining porous Mg-based structures is challenging due to their complexity and difficulties in achieving uniform or gradient porosity.This review aims to thoroughly explore various fabrication procedures used to create metallic scaffolds,with a specific focus on those made from Mg-based alloys.Both traditional manufacturing techniques,including the directional solidification of metal-gas eutectic technique,pattern casting,methods using space holders,and modern fabrication methods,which are based on additive manufacturing,are covered in this review article.Furthermore,the paper highlights the most important findings of recent studies on Mg-based scaffolds in terms of their microstructure specifications,mechanical properties,degradation and corrosion behavior,antibacterial activity,and biocompatibility(both in vivo and in vitro).While extensive research has been conducted to optimize manufacturing parameters and qualities of Mg-based scaffolds for use in biomedical applications,specifically for bone tissue engineering applications,further investigation is needed to fabricate these scaffolds with specific properties,such as high resistance to corrosion,good antibacterial properties,osteoconductivity,osteoinductivity,and the ability to elicit a favorable response from osteoblast-like cell lines.The review concludes with recommendations for future research in the field of medical applications.
基金financially funded by National Key Research and Development Program of China(2021YFC2400703)the National Nat-ural Science Foundation of China(NSFC)(52171234)+1 种基金Beijing Municipal Science and Technology Project(Z211100002921066)Peking Univer-sity National Clinical Key Specialty Translation Support Project(PKUSSNKP-Т202104).
文摘Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic prop-erties.However,conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts in vivo outcomes.In this study,we aimed to establish an in vitro evaluation model that better correlates with in vivo performance.Three-layer BMSC cell sheets were constructed using layer-by-layer assembly.Histological analyses revealed a stable three-dimensional structure with elevated cell-cell interaction proteins,including N-Cadherin,Fibronectin,and Vinculin,along with enhanced osteogenic potential.The cytotoxicity of 4N pure Mg was evaluated in both cell sheet and monolayer co-culture models.Flow cytometry showed higher Ki67 expression and lower ROS levels and apoptosis rate in cell sheets.ShRNA-mediated silencing of N-Cadherin in cell sheets significantly compromised their cytopro-tective capacity against Mg metal-induced toxicity.Osteogenesis-related gene expression correlation analysis between in vitro co-culture models and in vivo femur implantation models was conducted using RNA-seq and qRT-PCR.Results showed that 4N pure Mg enhanced osteogenic genes(BMP2R,RUNX2,and SP7)in cell sheets,consistent with in vivo patterns but contrary to monolayer models.Various Mg-based metals(4N/5N Pure Mg,ZE21B,and WE43)were evaluated in cell sheet defect,monolayer defect,and cranial defect models.5N Pure Mg,ZE21B,and WE43 promoted defect healing in both cranial defect and cell sheets,but showed no positive effect in monolayers.Collectively,cell sheet models correlated well with in vivo results,suggesting their potential as alternative in vitro evaluation models,thereby accelerating clinical translation of Mg-based biomaterials.
