The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'd...The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'dialogues from the perspective of politeness principle,one conception of which is that politeness is a social behavior in the contact of human beings,the strategies of Mabel's love pursuing and acquiring are gradually revealed.展开更多
Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herei...Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in tomatoes.Conversely, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.展开更多
第二代星载激光雷达冰、云和陆地测高卫星ICESat-2(The Ice, Cloud, and Land Elevation Satellite-2)搭载了先进光子计数式激光雷达,使用了全新的微脉冲多波束光子计数式激光雷达。由于光子计数式激光雷达的自身特点,其光子云数据具有...第二代星载激光雷达冰、云和陆地测高卫星ICESat-2(The Ice, Cloud, and Land Elevation Satellite-2)搭载了先进光子计数式激光雷达,使用了全新的微脉冲多波束光子计数式激光雷达。由于光子计数式激光雷达的自身特点,其光子云数据具有受噪声光子影响大、信噪比与扫描时间相关、光子分布密度不均匀等问题,目前开发的去噪算法并不能很好的应用于不同的光子云数据。基于以上问题,本文提出一种改进的去噪算法,首先分析光子云内部特征并自适应选择最优参数进行粗去噪,然后进行两次精去噪,最后对光子云进行分类并拟合出地面线及冠层顶线,为提取森林冠层高度提供基础。使用该算法对MABEL数据进行去噪实验,实验结果表明:该去噪算法的一次去噪对不同环境下MABEL数据在夜间的去噪平均精确度为94.5%,F1-score为96.3%,日间平均精确度为86.7%,F1-score为91.7%,且三次去噪算法完成后能够显著提升光子云去噪精度。实验证明该算法对MABEL光子云数据具有较好去噪效果和稳定性,可为ICESat-2数据处理提供参考。本文的光子分类算法能够从光子数据中提取冠层顶点、地面点及林内光子并在此算法中进一步精确去除剩余噪点,最终光子分类结果显示该算法能够从复杂光子云数据中提取森林剖面结构。展开更多
单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。...单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。使用NASA(National Aeronautics and Space Administration)的机载激光雷达MABEL(Multiple Altimeter Beam Experimental LiDAR)的实际飞行数据进行了实验,并通过模拟仿真的方法对实验结果做了验证。结果表明,此方法能有效地剔除噪声,试验区的整体精度能达到97.8%。展开更多
文摘The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'dialogues from the perspective of politeness principle,one conception of which is that politeness is a social behavior in the contact of human beings,the strategies of Mabel's love pursuing and acquiring are gradually revealed.
基金supported by the National Natural Science Foundation of China (grant no. 32202554)the earmarked fund for CARS (grant no. CARS-31)+3 种基金Guangdong Banana and Pineapple Industry Technology System Innovation Team(grant no. 2022KJ109)Natural Science Foundation of Guangdong Province (2023A1515010335)Pearl River Talent Program for Young Talent (grant no. 2017GC010321)Young Innovative Talents Projects in Ordinary Colleges and Universities in Guangdong Province (grant no.2021KQNCX005)。
文摘Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in tomatoes.Conversely, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.
文摘第二代星载激光雷达冰、云和陆地测高卫星ICESat-2(The Ice, Cloud, and Land Elevation Satellite-2)搭载了先进光子计数式激光雷达,使用了全新的微脉冲多波束光子计数式激光雷达。由于光子计数式激光雷达的自身特点,其光子云数据具有受噪声光子影响大、信噪比与扫描时间相关、光子分布密度不均匀等问题,目前开发的去噪算法并不能很好的应用于不同的光子云数据。基于以上问题,本文提出一种改进的去噪算法,首先分析光子云内部特征并自适应选择最优参数进行粗去噪,然后进行两次精去噪,最后对光子云进行分类并拟合出地面线及冠层顶线,为提取森林冠层高度提供基础。使用该算法对MABEL数据进行去噪实验,实验结果表明:该去噪算法的一次去噪对不同环境下MABEL数据在夜间的去噪平均精确度为94.5%,F1-score为96.3%,日间平均精确度为86.7%,F1-score为91.7%,且三次去噪算法完成后能够显著提升光子云去噪精度。实验证明该算法对MABEL光子云数据具有较好去噪效果和稳定性,可为ICESat-2数据处理提供参考。本文的光子分类算法能够从光子数据中提取冠层顶点、地面点及林内光子并在此算法中进一步精确去除剩余噪点,最终光子分类结果显示该算法能够从复杂光子云数据中提取森林剖面结构。
文摘单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。使用NASA(National Aeronautics and Space Administration)的机载激光雷达MABEL(Multiple Altimeter Beam Experimental LiDAR)的实际飞行数据进行了实验,并通过模拟仿真的方法对实验结果做了验证。结果表明,此方法能有效地剔除噪声,试验区的整体精度能达到97.8%。
