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香蕉MaERF15与MaACO8靶向结合调控果实采后成熟的分子机制 被引量:1
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作者 黄冰云 郑云柯 +2 位作者 张建斌 李新国 刘菊华 《热带作物学报》 北大核心 2025年第5期1032-1042,共11页
香蕉是典型的呼吸跃变型果实。MaERF15是定位于细胞核且具有调控香蕉果实采后成熟作用的转录因子,MaACO8是在香蕉果实采后成熟过程中高水平表达的1个乙烯生物合成关键酶-ACC氧化酶基因,但MaERF15和MaACO8的分子互作机制尚未明确。探究... 香蕉是典型的呼吸跃变型果实。MaERF15是定位于细胞核且具有调控香蕉果实采后成熟作用的转录因子,MaACO8是在香蕉果实采后成熟过程中高水平表达的1个乙烯生物合成关键酶-ACC氧化酶基因,但MaERF15和MaACO8的分子互作机制尚未明确。探究乙烯响应因子MaERF15对下游乙烯合成关键基因的转录调控机制,为香蕉果实采后保鲜技术的创新提供理论依据。本研究从香蕉DNA中分离出MaACO8启动子序列,通过生物信息学分析、GUS染色等方法研究MaACO8的启动子活性;采用酵母单杂交技术、EMSA、双荧光素报告系统和活体成像系统研究MaERF15与MaACO8启动子互作机制。结果表明:MaACO8的启动子序列有11个,与AP2/ERF转录因子的结合元件GCC-box具有较强的启动活性;MaERF15能与MaACO8启动子的GCC-box特异性结合,激活MaACO8的表达,促进乙烯的生物合成进而调控果实成熟。本研究结果进一步完善了香蕉果实采后乙烯生物合成调控果实成熟的理论,为香蕉耐贮运育种和成熟调控新技术的研发分别提供基因资源和理论依据。 展开更多
关键词 香蕉 MaERF15 maaco8 转录调控
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香蕉MaACO8基因启动子的克隆及活性分析
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作者 李雯倩 王卓 +3 位作者 贾彩红 王静毅 刘阳平 任羽 《热带农业科学》 2025年第12期77-86,共10页
香蕉(Musa spp.)是热带地区重要的经济作物之一,其果实成熟过程中受乙烯生物合成途径中关键酶1-氨基环丙烷-1-羧酸氧化酶(ACO)的调控。前期研究表明,MaACO8在果实成熟过程中表达量最大,推测其在果实成熟过程中起关键性作用。巴西蕉(Musa... 香蕉(Musa spp.)是热带地区重要的经济作物之一,其果实成熟过程中受乙烯生物合成途径中关键酶1-氨基环丙烷-1-羧酸氧化酶(ACO)的调控。前期研究表明,MaACO8在果实成熟过程中表达量最大,推测其在果实成熟过程中起关键性作用。巴西蕉(Musa acuminata L.,AAA group,cv.‘Cavendish’)为AAA型香蕉,有3个MaACO8的同源基因,为探寻3个MaACO8在巴西蕉果实成熟过程中的作用,本研究克隆并分析了巴西蕉中MaACO8-1、MaACO8-2和MaACO8-3基因的启动子序列。结果显示,MaACO8-1和MaACO8-2的启动子序列与MaACO8-3存在差异。生物信息学分析表明,MaACO8-1启动子比MaACO8-3启动子多一个光响应元件和一个脱落酸响应元件。GUS染色和酶活测定分析表明,MaACO8-1启动子比MaACO8-3启动子具有更高的活性。研究结果为不同基因型香蕉中ACO基因的表达模式及其在果实成熟过程中的调控机制研究提供理论依据。 展开更多
关键词 香蕉 maaco8基因 启动子 克隆 活性分析
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Characterization and expression profiles of MaACS and MaACO genes from mulberry(Morus alba L.)
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作者 Chang-ying LIU Rui-hua Lü +9 位作者 Jun LI Ai-chun ZHAO Xi-ling WANG Umuhoza DIANE Xiao-hong WANG Chuan-hong WANG Ya-sheng YU Shu-mei HAN Cheng LU Mao-de YU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2014年第7期611-623,共13页
1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of eth... 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal se-quences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demon-strated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon. 展开更多
关键词 Mulberry fruit MaACS maaco Gene expression Abscisic acid ETHEPHON
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Multi-agent and ant colony optimization for ship integrated power system network reconfiguration 被引量:6
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作者 WANG Zheng HU Zhiyuan YANG Xuanfang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2022年第2期489-496,共8页
Electric power is widely used as the main energy source of ship integrated power system(SIPS), which contains power network and electric power network. SIPS network reconfiguration is a non-linear large-scale problem.... Electric power is widely used as the main energy source of ship integrated power system(SIPS), which contains power network and electric power network. SIPS network reconfiguration is a non-linear large-scale problem. The reconfiguration solution influences the safety and stable operation of the power system. According to the operational characteristics of SIPS, a simplified model of power network and a mathematical model for network reconfiguration are established. Based on these models, a multi-agent and ant colony optimization(MAACO) is proposed to solve the problem of network reconfiguration. The simulations are carried out to demonstrate that the optimization method can reconstruct the integrated power system network accurately and efficiently. 展开更多
关键词 ship integrated power system(SIPS) multi-agent and ant colony optimization(maaco) network reconfiguration ring grid fault recovery
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