Transient receptor potential(TRP)channels are a class of ion channel proteins that are closely related to thermosensation in insects.They are involved in detecting the ambient temperature and play vital roles in insec...Transient receptor potential(TRP)channels are a class of ion channel proteins that are closely related to thermosensation in insects.They are involved in detecting the ambient temperature and play vital roles in insect survival and reproduction.In this study,we identifed and cloned two variants of the TRPA subfamily gene in Myzus persicae,MperTRPA1(A)and MperTRPA1(B),and analyzed their tissue expression by real-time quantitative PCR.Subsequently,these two variants of MperTRPA1 were expressed in the Xenopus oocyte system,and their functions were investigated using the two-electrode voltage clamp technique.The role of the MperTRPA1 gene in temperature adaptation of M.persicae was further determined by RNA interference and a behavioral choice assay to evaluate responses to temperature gradients.The results showed that the MperTRPA1 gene is widely expressed in tissues of M.persicae,with MperTRPA1(A)highly expressed in the mouthparts and MperTRPA1(B)mainly expressed in the antennae.The functional characterization results showed that both variants of MperTRPA1 could be activated and were not desensitized when the temperature increased from 20 to 45℃.The current value and thermal sensitivity(coeffcient Q_(10)value)of MperTRPA1(B)were signifcantly higher than those of MperTRPA1(A).When the MperTRPA1 gene was knocked down,the behavioral preference of M.persicae for the optimal temperature was reduced and tended to be at a higher temperature,showing a shift in the temperature adaptation range compared to both the wild type and ds GFP-treated M.persicae.In summary,our results elucidated the molecular mechanism of adaptive temperature perception in M.persicae mediated by the thermal sensor MperTRPA1.展开更多
The effects of semiochemicals extracted from 63 species of plants, on peach aphid (Myzus per-sicae) and mustard aphid (Lipaphis erysimi), were studied in laboratory. The deterrent rate, reproduction deterrent index an...The effects of semiochemicals extracted from 63 species of plants, on peach aphid (Myzus per-sicae) and mustard aphid (Lipaphis erysimi), were studied in laboratory. The deterrent rate, reproduction deterrent index and the interferential index of population control (IIPC) was used to evaluate the efficiency of semiochemicals on population control of the two target aphids. The results showed that the extracts of 34 species of common plants have noticeable effect on both aphid populations, especially, Xanthium sibiricum Petr. Et Widd. and Syngonium podophyllum Schott. These plant extracts could be used to construct the plant pro-tectant to protect crops.展开更多
In order to effectively predict occurrence quantity of Myzus persicae, BP neural network theory and method was used to establish prediction model for oc- currence quantity of M. persicae. Meanwhile, QPSO algorithm was...In order to effectively predict occurrence quantity of Myzus persicae, BP neural network theory and method was used to establish prediction model for oc- currence quantity of M. persicae. Meanwhile, QPSO algorithm was used to optimize connection weight and threshold value of BP neural network, so as to determine. the optimal connection weight and threshold value. The historical data of M. persica quantity in Hongta County, Yuxi City of Yunnan Province from 2003 to 2006 was adopted as training samples, and the occurrence quantities of M. persicae from 2007 to 2009 were predicted. The prediction accuracy was 99.35%, the mini- mum completion time was 30 s, the average completion time was 34.5 s, and the running times were 19. The prediction effect of the model was obviously superior to other prediction models. The experiment showed that this model was more effective and feasible, with faster convergence rate and stronger stability, and could solve the similar problems in prediction and clustering. The study provides a theoretical basis for comprehensive prevention and control against M. persicae.展开更多
In the research, 5% imidacloprid oil at different doses were applied to prevent Myzus persicae in order to explore significance analysis in 3, 7, 14, and 20 d, respectively, in three fields, and the results showed tha...In the research, 5% imidacloprid oil at different doses were applied to prevent Myzus persicae in order to explore significance analysis in 3, 7, 14, and 20 d, respectively, in three fields, and the results showed that 5% imidacloprid oil per- formed significant for K326, G28 and Yunyan-87, suggesting 5% imidacloprid oil is effective in controlling Myzus persicae.展开更多
A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard t...A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4- 10.4 conidia/mm^2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3 - 8.0 min) . Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1 - 12 h after exposure under temperature treatments of 15 and 20℃, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20℃ before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤ 1 h to infect > 50% of the aphids at a dosage of > 1.5 conida/mm^2 and > 90% at > 50 conidia/mm^2 .展开更多
For long-term prediction of occurrence degree of tobacco aphid Myzus persicae （Sulzer）, Markov chain method was used to establish prediction model for occurrence degree of tobacco aphid. With 4 levels of occurrence ...For long-term prediction of occurrence degree of tobacco aphid Myzus persicae （Sulzer）, Markov chain method was used to establish prediction model for occurrence degree of tobacco aphid. With 4 levels of occurrence degree, Markov chain model was established based on the data in 1987-2004. The results indicated that the accuracy for total prediction in 2005-2007 and the back prediction in 1987-2004 reached 88.89% and 85.12%, respectively. The method is simple and feasible for long-term prediction of occurrence degree of tobacco aphid.展开更多
[ Objective] The paper provided a reference for establishment of effective prevention mechanism against aphids in Zhenyuan tobacco area. [ Melllod ] The initial population quantity of aphids in early spring, migration...[ Objective] The paper provided a reference for establishment of effective prevention mechanism against aphids in Zhenyuan tobacco area. [ Melllod ] The initial population quantity of aphids in early spring, migration of winged aphids, occurrence regularity and damage status of Myzus persicae and aphid-transmit- ted virus （PVY ＋ CMV） were investigated in Zhenyuan County from 2014 to 2015 using 10-point checkerboard sampling method, yellow dish trapping method and 5-point sampling method. [ Result] The total amount of aphids in Brassica campestris was the largest among overwintering hosts, with an average of 18.44 speci- men/plant. The migration of winged aphids presented bimedal pattern; the population dynamics of wingless aphids presented unimodal pattern, and the occurrence peak was from May to June. [ Conclusion] Field control against M. persicae in tobacco fields should be conducted from mid May to early June.展开更多
The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the pa...The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the parasitic rate of M. persicae before A. gifuensis release was 0. 85% in field,and that at 20 d after A. gifuensis release was 59. 49%. The incidence rate of aphid-transmitted virus disease was controlled at 0. 62%. The release of A. gifuensis increased its natural population quantity in field,and had obvious direct control effects against M. persicae. Meantime,it indirectly controlled the occurrence of aphid-transmitted virus disease to some extent.展开更多
The green peach aphid, Myzuspersicae (Sulzer), is a major insect pest of many agronomic and horticultural crops and is distributed worldwide. Aphid management is often based on application of insecticides. However, th...The green peach aphid, Myzuspersicae (Sulzer), is a major insect pest of many agronomic and horticultural crops and is distributed worldwide. Aphid management is often based on application of insecticides. However, the aphid is now resistant to many of these and much interest has recently developed in identification of novel alternative insecticides. Venom isolated from the red imported fire ant, Solenopsisinvicta Buren, is composed of two groups of alkaloids, piperidines and piperideines, and has shown activity against many organisms including fungi, bacteria, mites and several insects. Prior to the study reported herein, no information on the venom’s activity to Myzuspersicae has been reported. Both of the alkaloids were active against M.persicae. The 24 h LC50 values were116.6 and 91.5 ppm for the piperideine and piperidine extracts, respectively. Based on overlap of the 95% fiducial limits the LC50 values for the two alkaloids did not significantly differ. At the high dosages, mortality occurred in as few as four hours and all treated aphids were dead by six hours. Little additional mortality was detected in the 48-hobservation. Both piperideine and piperidine extracts isolated from red imported fire ant venom possess sufficient activity to cause death of green peach aphids and at high dosages, death occurs rapidly. In the search for new replacements to synthetic insecticides, these extracts may offer a novel but potentially successful alternative.展开更多
Ultraviolet(UV)radiation,an environmental stressor,is crucial for the survival and adaptation of organisms.Myzus persicae,a global pest,is exposed to sunlight yearround,making it unable to avoid UV rays in its environ...Ultraviolet(UV)radiation,an environmental stressor,is crucial for the survival and adaptation of organisms.Myzus persicae,a global pest,is exposed to sunlight yearround,making it unable to avoid UV rays in its environment.MicroRNAs(miRNAs)are important posttranscriptional regulators of gene expression and mediate various biological processes.However,the role of miRNA in aphids in response to UV-B stress is unclear.In this study,Mpp53 expression level significantly increased with an increase in the duration of UV-B radiation,peaking at 2 h;knockdown of Mpp53 decreased the survival rate of aphids under UV-B stress,suggesting that Mpp53 is involved in aphid responses to UV-B.Here,we first predicted 8 miRNAs targeting Mpp53,and then screened for miRNAs related to UV-B resistance in aphids;of these,5 miRNAs(miR-305-5p,novel_50,novel_80,novel_166,and novel_61)were found to target Mpp53.Luciferase reporter assays demonstrated that novel_61 binds to the noncoding region of Mpp53 and downregulates its expression.Overexpression of novel_61 in aphids decreased Mpp53 expression and caused significant mortality under UV-B irradiation.Furthermore,the aphids exhibited lower reproductive capacity,lower body weight,and shorter body length and width.This is the first study to systematically screen and identify miRNA related to aphid responses to UVB stress and deepens our understanding of the molecular mechanism of insect responses to environmental stress,which may eventually aid in developing better control strategies.展开更多
The alates of the green peach aphid, Myzus persicae, were daily trapped from the air from late October through early January and carried to laboratory for determination of fungal infection by individually rearing them...The alates of the green peach aphid, Myzus persicae, were daily trapped from the air from late October through early January and carried to laboratory for determination of fungal infection by individually rearing them for 7 d on detached cabbage leaves in Petri dishes. Among 760 alates trapped, 266 (35%) were found carrying various fungal pathogens, 87.3% of them died due to mycosis during the first 3-day period of rearing and the rest died in the following two days. Most of the deaths of the alates were attributed to entomophthoralean fungi, taking 94.4%, and the rest were the hyphomycetous fungus Beauveria bassiana. Among the Entomophthorales-killed alates, P. neoaphidis took a proportion of 66.1%, Z. anhuiensis 22.6%, E. planchoniana 9.7%, and N. fresenii 1.6%, respectively. Two alates were found suffering from cross infection of two fungal species, i.e. P. neoaphidis with Z. anhuiensis and N. fresenii, respectively. The results represent the first report on transmission of aphid-pathogenic fungi展开更多
The green peach aphid, Myzus persicae Sulzer （Hemiptera, Aphididae）, is an important cosmopolitan pest. Real time qRT-PCR has been used for target gene expression analysis on M. persicae. Using real time qRT-PCR, th...The green peach aphid, Myzus persicae Sulzer （Hemiptera, Aphididae）, is an important cosmopolitan pest. Real time qRT-PCR has been used for target gene expression analysis on M. persicae. Using real time qRT-PCR, the expression levels are normalized on the basis of the reliable reference genes. However, to date, the stability of available reference genes has been insufficient. In this study, we evaluated nine candidate reference genes from M. persicae under diverse experimental conditions. The tested candidate genes were comprehensively ranked based on five alternative methods （RefFinder, geNorm, Normfinder, BestKeeper and the comparative ACt method）. 18s, Actin and ribosomal protein L27 （L27） were recommended as the most stable reference genes for M. persicae, whereas ribosomal protein L27 （L27） was found to be the least stable reference genes for abiotic studies （photoperiod, temperature and insecticide susceptibility）. Our finding not only sheds light on establishing an accurate and reliable normalization of real time qRT-PCR data in M. persicae but also lays a solid foundation for further studies of M. persicae involving RNA interference and functional gene research.展开更多
Insect CAPA neuropeptidesare considered to affect water and ion balance by mediating the physiological metabolism activities of the Malpighian tubules.In previous studies,the CAPA-PK analogue 1895(2Abf-Suc-FGPRLamide)...Insect CAPA neuropeptidesare considered to affect water and ion balance by mediating the physiological metabolism activities of the Malpighian tubules.In previous studies,the CAPA-PK analogue 1895(2Abf-Suc-FGPRLamide)was reported to decrease aphid fitness when administered through microinjection or via topical application.However,a further statistically significant decrease in the fitness of aphids and an increased mortality could not be established with pairwise combinations of 1895 with other CAPA analogue.In this study,we assessed the topical application of new combinations of 1895 with five CAPA-PVK analogues on the fitness of aphids.We found that 1895 and CAPA-PVK analogue 2315(ASG-[β3L]-VAFPRVamide)was statistically the most effective combination to control the peach potato aphid Myzus persicae nymphs via topical application,leading to 72%mortality.Additionally,the combination(1895+2315)was evaluated against a selection of beneficial insects,that is,a pollinator(Bombus terrestris)and three natural enemies(Chrysoperla carnea,Nasonia vitripennis,and Adalia bipunctata).We found no significant influence on food intake,weight increase,and survival for the pollinator and the three representative natural enemies.These results could facilitate to further establish and generate CAPA analogues as alternatives to broad spectrum and less friendly insecticides.展开更多
In a tritrophic context of plant-insect-entomopathogen,plants play important roles in modulating the interaction of insects and their pathogenic viruses.Currently,the influence of plants on the transmission of insect ...In a tritrophic context of plant-insect-entomopathogen,plants play important roles in modulating the interaction of insects and their pathogenic viruses.Currently,the influence of plants on the transmission of insect viruses has been mainly studied on baculoviruses and some RNA viruses,whereas the impact of plants on other insect viruses is largely unknown.Here,we identified a new densovirus infecting the green peach aphid Myzus persicae and tested whether and how host plants influence the transmission of the aphid densovirus.The complete single-stranded DNA genome of the virus,M.persicae densovirus 2,is 5727 nt and contains inverted terminal repeats.Transcription and phylogenetic analysis indicated that the virus was distinct from other a few identified aphid densoviruses.The virus abundance was detected highly in the intestinal tract of aphids,compared with the lower level of it in other tissues including head,embryo,and epidermis.Cabbage and pepper plants had no obvious effect on the vertical transmission and saliva-mediated horizontal transmission of the virus.However,the honeydew-mediated horizontal transmission among aphids highly depended on host plants(65%on cabbages versus 17%on peppers).Although the virus concentration in the honeydew produced by aphids between 2 plants was similar,the honeydew production of the infected aphids reared on peppers was dramatically reduced.Taken together,our results provide evidence that plants influence the horizontal transmission of a new densovirus in an aphid population by modulating honeydew secretion of aphids,suggesting plants may manipulate the spread of an aphid-pathogenic densovirus in nature.展开更多
基金funded by the National Natural Science Foundation of China(32472553 and 31872039)the Major Special Projects for Green Pest Control,China(110202201017(LS-01))+1 种基金the Shenzhen Science and Technology Program,China(KQTD20180411143628272)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘Transient receptor potential(TRP)channels are a class of ion channel proteins that are closely related to thermosensation in insects.They are involved in detecting the ambient temperature and play vital roles in insect survival and reproduction.In this study,we identifed and cloned two variants of the TRPA subfamily gene in Myzus persicae,MperTRPA1(A)and MperTRPA1(B),and analyzed their tissue expression by real-time quantitative PCR.Subsequently,these two variants of MperTRPA1 were expressed in the Xenopus oocyte system,and their functions were investigated using the two-electrode voltage clamp technique.The role of the MperTRPA1 gene in temperature adaptation of M.persicae was further determined by RNA interference and a behavioral choice assay to evaluate responses to temperature gradients.The results showed that the MperTRPA1 gene is widely expressed in tissues of M.persicae,with MperTRPA1(A)highly expressed in the mouthparts and MperTRPA1(B)mainly expressed in the antennae.The functional characterization results showed that both variants of MperTRPA1 could be activated and were not desensitized when the temperature increased from 20 to 45℃.The current value and thermal sensitivity(coeffcient Q_(10)value)of MperTRPA1(B)were signifcantly higher than those of MperTRPA1(A).When the MperTRPA1 gene was knocked down,the behavioral preference of M.persicae for the optimal temperature was reduced and tended to be at a higher temperature,showing a shift in the temperature adaptation range compared to both the wild type and ds GFP-treated M.persicae.In summary,our results elucidated the molecular mechanism of adaptive temperature perception in M.persicae mediated by the thermal sensor MperTRPA1.
文摘The effects of semiochemicals extracted from 63 species of plants, on peach aphid (Myzus per-sicae) and mustard aphid (Lipaphis erysimi), were studied in laboratory. The deterrent rate, reproduction deterrent index and the interferential index of population control (IIPC) was used to evaluate the efficiency of semiochemicals on population control of the two target aphids. The results showed that the extracts of 34 species of common plants have noticeable effect on both aphid populations, especially, Xanthium sibiricum Petr. Et Widd. and Syngonium podophyllum Schott. These plant extracts could be used to construct the plant pro-tectant to protect crops.
基金Supported by Science and Technology Project of China National Tobacco Corporation(2009YN005&2010YN18&2010YN19)
文摘In order to effectively predict occurrence quantity of Myzus persicae, BP neural network theory and method was used to establish prediction model for oc- currence quantity of M. persicae. Meanwhile, QPSO algorithm was used to optimize connection weight and threshold value of BP neural network, so as to determine. the optimal connection weight and threshold value. The historical data of M. persica quantity in Hongta County, Yuxi City of Yunnan Province from 2003 to 2006 was adopted as training samples, and the occurrence quantities of M. persicae from 2007 to 2009 were predicted. The prediction accuracy was 99.35%, the mini- mum completion time was 30 s, the average completion time was 34.5 s, and the running times were 19. The prediction effect of the model was obviously superior to other prediction models. The experiment showed that this model was more effective and feasible, with faster convergence rate and stronger stability, and could solve the similar problems in prediction and clustering. The study provides a theoretical basis for comprehensive prevention and control against M. persicae.
文摘In the research, 5% imidacloprid oil at different doses were applied to prevent Myzus persicae in order to explore significance analysis in 3, 7, 14, and 20 d, respectively, in three fields, and the results showed that 5% imidacloprid oil per- formed significant for K326, G28 and Yunyan-87, suggesting 5% imidacloprid oil is effective in controlling Myzus persicae.
文摘A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4- 10.4 conidia/mm^2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3 - 8.0 min) . Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1 - 12 h after exposure under temperature treatments of 15 and 20℃, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20℃ before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤ 1 h to infect > 50% of the aphids at a dosage of > 1.5 conida/mm^2 and > 90% at > 50 conidia/mm^2 .
基金Supported by Tobacco Monopoly Bureau of Shandong Province (KN90)
文摘For long-term prediction of occurrence degree of tobacco aphid Myzus persicae （Sulzer）, Markov chain method was used to establish prediction model for occurrence degree of tobacco aphid. With 4 levels of occurrence degree, Markov chain model was established based on the data in 1987-2004. The results indicated that the accuracy for total prediction in 2005-2007 and the back prediction in 1987-2004 reached 88.89% and 85.12%, respectively. The method is simple and feasible for long-term prediction of occurrence degree of tobacco aphid.
基金Supported by Science and Technology Project of Southeast Guizhou in 2013 "Research and Application of Prevention and Control Technology against Myzus persicae with Aphidius gifuensis" (2013)
文摘[ Objective] The paper provided a reference for establishment of effective prevention mechanism against aphids in Zhenyuan tobacco area. [ Melllod ] The initial population quantity of aphids in early spring, migration of winged aphids, occurrence regularity and damage status of Myzus persicae and aphid-transmit- ted virus （PVY ＋ CMV） were investigated in Zhenyuan County from 2014 to 2015 using 10-point checkerboard sampling method, yellow dish trapping method and 5-point sampling method. [ Result] The total amount of aphids in Brassica campestris was the largest among overwintering hosts, with an average of 18.44 speci- men/plant. The migration of winged aphids presented bimedal pattern; the population dynamics of wingless aphids presented unimodal pattern, and the occurrence peak was from May to June. [ Conclusion] Field control against M. persicae in tobacco fields should be conducted from mid May to early June.
文摘The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the parasitic rate of M. persicae before A. gifuensis release was 0. 85% in field,and that at 20 d after A. gifuensis release was 59. 49%. The incidence rate of aphid-transmitted virus disease was controlled at 0. 62%. The release of A. gifuensis increased its natural population quantity in field,and had obvious direct control effects against M. persicae. Meantime,it indirectly controlled the occurrence of aphid-transmitted virus disease to some extent.
文摘The green peach aphid, Myzuspersicae (Sulzer), is a major insect pest of many agronomic and horticultural crops and is distributed worldwide. Aphid management is often based on application of insecticides. However, the aphid is now resistant to many of these and much interest has recently developed in identification of novel alternative insecticides. Venom isolated from the red imported fire ant, Solenopsisinvicta Buren, is composed of two groups of alkaloids, piperidines and piperideines, and has shown activity against many organisms including fungi, bacteria, mites and several insects. Prior to the study reported herein, no information on the venom’s activity to Myzuspersicae has been reported. Both of the alkaloids were active against M.persicae. The 24 h LC50 values were116.6 and 91.5 ppm for the piperideine and piperidine extracts, respectively. Based on overlap of the 95% fiducial limits the LC50 values for the two alkaloids did not significantly differ. At the high dosages, mortality occurred in as few as four hours and all treated aphids were dead by six hours. Little additional mortality was detected in the 48-hobservation. Both piperideine and piperidine extracts isolated from red imported fire ant venom possess sufficient activity to cause death of green peach aphids and at high dosages, death occurs rapidly. In the search for new replacements to synthetic insecticides, these extracts may offer a novel but potentially successful alternative.
基金supported by the Guizhou Provincial Department of Education Program(Grant number:KY[2021]057)the Guizhou Tobacco Company Program(Grant Numbers:2021XM09 and 2022XM09)+2 种基金the Major Project of the China National Tobacco Corporation[110202201020(LS-04)]the Major Special Project of Guizhou of China Tobacco Corporation(Grant Number:2023XM07)the Zunyi Tobacco Company Program(Grant number:2022XM12).
文摘Ultraviolet(UV)radiation,an environmental stressor,is crucial for the survival and adaptation of organisms.Myzus persicae,a global pest,is exposed to sunlight yearround,making it unable to avoid UV rays in its environment.MicroRNAs(miRNAs)are important posttranscriptional regulators of gene expression and mediate various biological processes.However,the role of miRNA in aphids in response to UV-B stress is unclear.In this study,Mpp53 expression level significantly increased with an increase in the duration of UV-B radiation,peaking at 2 h;knockdown of Mpp53 decreased the survival rate of aphids under UV-B stress,suggesting that Mpp53 is involved in aphid responses to UV-B.Here,we first predicted 8 miRNAs targeting Mpp53,and then screened for miRNAs related to UV-B resistance in aphids;of these,5 miRNAs(miR-305-5p,novel_50,novel_80,novel_166,and novel_61)were found to target Mpp53.Luciferase reporter assays demonstrated that novel_61 binds to the noncoding region of Mpp53 and downregulates its expression.Overexpression of novel_61 in aphids decreased Mpp53 expression and caused significant mortality under UV-B irradiation.Furthermore,the aphids exhibited lower reproductive capacity,lower body weight,and shorter body length and width.This is the first study to systematically screen and identify miRNA related to aphid responses to UVB stress and deepens our understanding of the molecular mechanism of insect responses to environmental stress,which may eventually aid in developing better control strategies.
基金This work was partially supported by the "Cheung Kong Scholars Programme', Ministry of Education, P. R. Chinathe National Natural Science Foundation of China (Grant Nos. 30070514 and 39870513).
文摘The alates of the green peach aphid, Myzus persicae, were daily trapped from the air from late October through early January and carried to laboratory for determination of fungal infection by individually rearing them for 7 d on detached cabbage leaves in Petri dishes. Among 760 alates trapped, 266 (35%) were found carrying various fungal pathogens, 87.3% of them died due to mycosis during the first 3-day period of rearing and the rest died in the following two days. Most of the deaths of the alates were attributed to entomophthoralean fungi, taking 94.4%, and the rest were the hyphomycetous fungus Beauveria bassiana. Among the Entomophthorales-killed alates, P. neoaphidis took a proportion of 66.1%, Z. anhuiensis 22.6%, E. planchoniana 9.7%, and N. fresenii 1.6%, respectively. Two alates were found suffering from cross infection of two fungal species, i.e. P. neoaphidis with Z. anhuiensis and N. fresenii, respectively. The results represent the first report on transmission of aphid-pathogenic fungi
文摘The green peach aphid, Myzus persicae Sulzer （Hemiptera, Aphididae）, is an important cosmopolitan pest. Real time qRT-PCR has been used for target gene expression analysis on M. persicae. Using real time qRT-PCR, the expression levels are normalized on the basis of the reliable reference genes. However, to date, the stability of available reference genes has been insufficient. In this study, we evaluated nine candidate reference genes from M. persicae under diverse experimental conditions. The tested candidate genes were comprehensively ranked based on five alternative methods （RefFinder, geNorm, Normfinder, BestKeeper and the comparative ACt method）. 18s, Actin and ribosomal protein L27 （L27） were recommended as the most stable reference genes for M. persicae, whereas ribosomal protein L27 （L27） was found to be the least stable reference genes for abiotic studies （photoperiod, temperature and insecticide susceptibility）. Our finding not only sheds light on establishing an accurate and reliable normalization of real time qRT-PCR data in M. persicae but also lays a solid foundation for further studies of M. persicae involving RNA interference and functional gene research.
基金The authors also acknowledge funding from the Euro・pean Union's Horizon 2020 Research and Innovation program under grant agreement No.634361(nEU・ROSTRESSPEP)This research was also partially supported by the Special Research Fund of Ghent University(BOF-UGent)and the Research Foundation-Flanders(FWO-Vlaanderen).
文摘Insect CAPA neuropeptidesare considered to affect water and ion balance by mediating the physiological metabolism activities of the Malpighian tubules.In previous studies,the CAPA-PK analogue 1895(2Abf-Suc-FGPRLamide)was reported to decrease aphid fitness when administered through microinjection or via topical application.However,a further statistically significant decrease in the fitness of aphids and an increased mortality could not be established with pairwise combinations of 1895 with other CAPA analogue.In this study,we assessed the topical application of new combinations of 1895 with five CAPA-PVK analogues on the fitness of aphids.We found that 1895 and CAPA-PVK analogue 2315(ASG-[β3L]-VAFPRVamide)was statistically the most effective combination to control the peach potato aphid Myzus persicae nymphs via topical application,leading to 72%mortality.Additionally,the combination(1895+2315)was evaluated against a selection of beneficial insects,that is,a pollinator(Bombus terrestris)and three natural enemies(Chrysoperla carnea,Nasonia vitripennis,and Adalia bipunctata).We found no significant influence on food intake,weight increase,and survival for the pollinator and the three representative natural enemies.These results could facilitate to further establish and generate CAPA analogues as alternatives to broad spectrum and less friendly insecticides.
基金This work was supported by Ministry of Education of the People's Republic of China Chinese Universities Scientific Fund(No.Z1090121096,NWAFU)Ministry of Science and Technology of the People's Republic of China National Key R&D Program of China(2017YFC1200605).
文摘In a tritrophic context of plant-insect-entomopathogen,plants play important roles in modulating the interaction of insects and their pathogenic viruses.Currently,the influence of plants on the transmission of insect viruses has been mainly studied on baculoviruses and some RNA viruses,whereas the impact of plants on other insect viruses is largely unknown.Here,we identified a new densovirus infecting the green peach aphid Myzus persicae and tested whether and how host plants influence the transmission of the aphid densovirus.The complete single-stranded DNA genome of the virus,M.persicae densovirus 2,is 5727 nt and contains inverted terminal repeats.Transcription and phylogenetic analysis indicated that the virus was distinct from other a few identified aphid densoviruses.The virus abundance was detected highly in the intestinal tract of aphids,compared with the lower level of it in other tissues including head,embryo,and epidermis.Cabbage and pepper plants had no obvious effect on the vertical transmission and saliva-mediated horizontal transmission of the virus.However,the honeydew-mediated horizontal transmission among aphids highly depended on host plants(65%on cabbages versus 17%on peppers).Although the virus concentration in the honeydew produced by aphids between 2 plants was similar,the honeydew production of the infected aphids reared on peppers was dramatically reduced.Taken together,our results provide evidence that plants influence the horizontal transmission of a new densovirus in an aphid population by modulating honeydew secretion of aphids,suggesting plants may manipulate the spread of an aphid-pathogenic densovirus in nature.
