Stomata play critical roles in gas exchange and immunity to pathogens.While many genes regulating early stomatal development up to the production of young guard cells(GCs)have been identified in Arabidopsis,much less ...Stomata play critical roles in gas exchange and immunity to pathogens.While many genes regulating early stomatal development up to the production of young guard cells(GCs)have been identified in Arabidopsis,much less is known about how young GCs develop into mature functional stomata.Here we perform a maturomics study on stomata,with“maturomics”defined as omics analysis of the maturation process of a tissue or organ.We develop an integrative scheme to analyze three public stomata-related single-cell RNAseq datasets and identify a list of 586 genes that are specifically up-regulated in all three datasets during stomatal maturation and function formation.The list,termed sc_586,is enriched with known regulators of stomatal maturation and functions.To validate the reliability of the dataset,we selected two candidate G2-like transcription factor genes,MYS1 and MYS2,to investigate their roles in stomata.These two genes redundantly regulate the size and hoop rigidity of mature GCs,and the mys1 mys2 double mutants cause mature GCs with severe defects in regulating their stomatal apertures.Taken together,our results provide a valuable list of genes for studying GC maturation and function formation.展开更多
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Science(XDA24010303)the National Natural Science Foundation of China(31770268)+1 种基金the Fundamental Research Funds for the Central Universities(WK2070000091)the University of Science and Technology of China(Start-up fund to S.M.)。
文摘Stomata play critical roles in gas exchange and immunity to pathogens.While many genes regulating early stomatal development up to the production of young guard cells(GCs)have been identified in Arabidopsis,much less is known about how young GCs develop into mature functional stomata.Here we perform a maturomics study on stomata,with“maturomics”defined as omics analysis of the maturation process of a tissue or organ.We develop an integrative scheme to analyze three public stomata-related single-cell RNAseq datasets and identify a list of 586 genes that are specifically up-regulated in all three datasets during stomatal maturation and function formation.The list,termed sc_586,is enriched with known regulators of stomatal maturation and functions.To validate the reliability of the dataset,we selected two candidate G2-like transcription factor genes,MYS1 and MYS2,to investigate their roles in stomata.These two genes redundantly regulate the size and hoop rigidity of mature GCs,and the mys1 mys2 double mutants cause mature GCs with severe defects in regulating their stomatal apertures.Taken together,our results provide a valuable list of genes for studying GC maturation and function formation.
