Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistan...Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistance to salt stress.In this study,we used corn steep liquor(CSL),myo-inositol(MI),and their combination to improve salt tolerance in Chinese cabbage(Brassica rapa L.ssp.pekinensis)under salt stress conditions.All three treatments significantly increased plant biomass and nutrient uptake,and improved soil physicochemical properties,while alleviating oxidative damage and ion toxicity.展开更多
目的明确肌球蛋白1B(MYO1B)在胃癌组织中的表达情况,并分析其对患者远期预后的评估价值以及对肿瘤细胞恶性生物学行为的影响和可能的机制。方法通过癌症公共数据库预测MYO1B在胃癌中的表达水平、与肿瘤分级、分期的相关性及对患者生存...目的明确肌球蛋白1B(MYO1B)在胃癌组织中的表达情况,并分析其对患者远期预后的评估价值以及对肿瘤细胞恶性生物学行为的影响和可能的机制。方法通过癌症公共数据库预测MYO1B在胃癌中的表达水平、与肿瘤分级、分期的相关性及对患者生存情况的影响。纳入在我院接受胃癌根治术的患者105例,采用免疫组化技术检测MYO1B在胃癌和癌旁组织中的表达情况,分析其与胃癌恶性进展参数的关系,以及对患者术后5年生存率的影响和评估价值。通过GO和KEGG富集分析MYO1B可能作用于胃癌的生物学功能和途径。体外探究MYO1B差异表达对胃癌细胞增殖、迁移和侵袭的作用及机制。结果癌症公共数据库显示胃癌组织中MYO1B表达水平高于正常组织,与肿瘤分级和分期有关并影响患者预后(P<0.05)。MYO1B在胃癌组织中高表达且与Ki67表达正相关(r=0.689,P<0.05)。MYO1B高表达与胃癌恶性进展参数(CEA≥5μg/L、CA19-9≥37 kU/L、G3~4、T3~4及N2~3)相关(P<0.05)。Kaplan-Meier生存分析和多因素Cox回归显示,MYO1B高表达降低患者术后5年生存率且为独立危险因素(HR:3.522,95%CI:1.783~6.985,P<0.05)。ROC曲线反映,MYO1B表达水平对患者术后生存情况的预测能力较强(Cut off value:3.11,AUC:0.753,P<0.05)。MYO1B可能与细胞迁移和mTOR信号通路的调控有关(P<0.05)。上调MYO1B促进胃癌细胞增殖、迁移和侵袭能力(P<0.05)。体外过表达MYO1B可促进信号分子Akt、mTOR的磷酸化(P<0.05)。结论MYO1B高表达可显著促进胃癌细胞增殖、迁移和侵袭,且与患者预后不良有关。展开更多
Myosin XVIIIA, or MYO18A, is a unique PDZ domain-containing unconventional myosin and is evolutionarily conserved from Drosophila to vertebrates. Although there is evidence indicating its expression in the somites, wh...Myosin XVIIIA, or MYO18A, is a unique PDZ domain-containing unconventional myosin and is evolutionarily conserved from Drosophila to vertebrates. Although there is evidence indicating its expression in the somites, whether it regulates muscle function re- mains unclear. We show that the two zebrafish myo18a genes （myo18aa and myo18ab） are predominantly expressed at somite borders during early developmental stages. Knockdown of these genes or overexpression of the MYO18A PDZ domain disrupts myofiber integrity, induces myofiber lesions, and compromises the localization of dystrophin, ^-dystroglycan （^-DG） and laminin at the myotome boundaries. Cell transplantation experiments indicate that myo18a morphant myoblasts fail to form elongated myofibers in the myotomes of wild-type embryos, which can be rescued by the full-length MYO18A protein. These results suggest that MYO18A likely functions in the adhesion process that maintains the stable attachment of myofibers to ECM （extracellular matrix） and muscle integrity during early development.展开更多
目的:分析慢性心力衰竭患者血清肌酸激酶同工酶(Creatine Kinase-MyocardialBand,CK-MB)、肌红蛋白(Myoglobin,MyO)、乳酸脱氢酶(Lactate Dehydrogenase,LDH)水平与心衰严重程度及近期预后的相关性。方法:选取我院2022年6月-2024年4月15...目的:分析慢性心力衰竭患者血清肌酸激酶同工酶(Creatine Kinase-MyocardialBand,CK-MB)、肌红蛋白(Myoglobin,MyO)、乳酸脱氢酶(Lactate Dehydrogenase,LDH)水平与心衰严重程度及近期预后的相关性。方法:选取我院2022年6月-2024年4月159例慢性心力衰竭患者为研究对象,根据预后3个月是否发生不良心血管事件(Major adverse cardiovascularevents,MACE)分为MACE患者及非MACE患者;对比不同心功能(New York Heart Association Functional Classification,NYHA)分级及不同预后患者血清CK-MB、MyO、LDH水平,分析其与NYHA分级相关性及对预后发生MACE的预测价值和危险度。结果:入院时各组血清CK-MB、MyO、LDH水平比较,I级组<I级组<Ⅲ级组<IV级组(P<0.05);血清CK-MB、MyO、LDH均与NYHA分级呈正相关(P<0.05);入院时及治疗1周后MACE患者血清CK-MB、MyO、LDH水平均高于非MACE患者(P<0.05);入院时及治疗1周后血清CK-MB、MyO、LDH水平联合诊断的曲线下面积(Area Under the Curve,AUC)分别为0.752、0.794(P<0.05);入院时、治疗1周后血清CK-MB、MyO、LDH高水平患者发生MACE的危险度是低水平患者的数倍(P<0.05)。结论:血清CK-MB、MyO、LDH水平与慢性心力衰竭患者病情程度及预后相关,临床检测其水平对评估患者近期预后具有重要价值。展开更多
基金supported by the sub-project“Research and Application of In-Situ Value-Added Water-Soluble Fertilizer Application Technology”(Grant No.2023YFD1700204-3)under the 14th Five-Year National Key R&D Program Project“Development and Industrialization of Novel Green Value-Added Fertilizers”.
文摘Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistance to salt stress.In this study,we used corn steep liquor(CSL),myo-inositol(MI),and their combination to improve salt tolerance in Chinese cabbage(Brassica rapa L.ssp.pekinensis)under salt stress conditions.All three treatments significantly increased plant biomass and nutrient uptake,and improved soil physicochemical properties,while alleviating oxidative damage and ion toxicity.
文摘目的明确肌球蛋白1B(MYO1B)在胃癌组织中的表达情况,并分析其对患者远期预后的评估价值以及对肿瘤细胞恶性生物学行为的影响和可能的机制。方法通过癌症公共数据库预测MYO1B在胃癌中的表达水平、与肿瘤分级、分期的相关性及对患者生存情况的影响。纳入在我院接受胃癌根治术的患者105例,采用免疫组化技术检测MYO1B在胃癌和癌旁组织中的表达情况,分析其与胃癌恶性进展参数的关系,以及对患者术后5年生存率的影响和评估价值。通过GO和KEGG富集分析MYO1B可能作用于胃癌的生物学功能和途径。体外探究MYO1B差异表达对胃癌细胞增殖、迁移和侵袭的作用及机制。结果癌症公共数据库显示胃癌组织中MYO1B表达水平高于正常组织,与肿瘤分级和分期有关并影响患者预后(P<0.05)。MYO1B在胃癌组织中高表达且与Ki67表达正相关(r=0.689,P<0.05)。MYO1B高表达与胃癌恶性进展参数(CEA≥5μg/L、CA19-9≥37 kU/L、G3~4、T3~4及N2~3)相关(P<0.05)。Kaplan-Meier生存分析和多因素Cox回归显示,MYO1B高表达降低患者术后5年生存率且为独立危险因素(HR:3.522,95%CI:1.783~6.985,P<0.05)。ROC曲线反映,MYO1B表达水平对患者术后生存情况的预测能力较强(Cut off value:3.11,AUC:0.753,P<0.05)。MYO1B可能与细胞迁移和mTOR信号通路的调控有关(P<0.05)。上调MYO1B促进胃癌细胞增殖、迁移和侵袭能力(P<0.05)。体外过表达MYO1B可促进信号分子Akt、mTOR的磷酸化(P<0.05)。结论MYO1B高表达可显著促进胃癌细胞增殖、迁移和侵袭,且与患者预后不良有关。
基金supported by the grants from the National Science Foundation of China (No. 31271556)Association Francaise contre les Myopathies (AFM)the Agence Nationale de la Recherche (ANR-09-BLAN-0262-03) in France
文摘Myosin XVIIIA, or MYO18A, is a unique PDZ domain-containing unconventional myosin and is evolutionarily conserved from Drosophila to vertebrates. Although there is evidence indicating its expression in the somites, whether it regulates muscle function re- mains unclear. We show that the two zebrafish myo18a genes （myo18aa and myo18ab） are predominantly expressed at somite borders during early developmental stages. Knockdown of these genes or overexpression of the MYO18A PDZ domain disrupts myofiber integrity, induces myofiber lesions, and compromises the localization of dystrophin, ^-dystroglycan （^-DG） and laminin at the myotome boundaries. Cell transplantation experiments indicate that myo18a morphant myoblasts fail to form elongated myofibers in the myotomes of wild-type embryos, which can be rescued by the full-length MYO18A protein. These results suggest that MYO18A likely functions in the adhesion process that maintains the stable attachment of myofibers to ECM （extracellular matrix） and muscle integrity during early development.
文摘目的:分析慢性心力衰竭患者血清肌酸激酶同工酶(Creatine Kinase-MyocardialBand,CK-MB)、肌红蛋白(Myoglobin,MyO)、乳酸脱氢酶(Lactate Dehydrogenase,LDH)水平与心衰严重程度及近期预后的相关性。方法:选取我院2022年6月-2024年4月159例慢性心力衰竭患者为研究对象,根据预后3个月是否发生不良心血管事件(Major adverse cardiovascularevents,MACE)分为MACE患者及非MACE患者;对比不同心功能(New York Heart Association Functional Classification,NYHA)分级及不同预后患者血清CK-MB、MyO、LDH水平,分析其与NYHA分级相关性及对预后发生MACE的预测价值和危险度。结果:入院时各组血清CK-MB、MyO、LDH水平比较,I级组<I级组<Ⅲ级组<IV级组(P<0.05);血清CK-MB、MyO、LDH均与NYHA分级呈正相关(P<0.05);入院时及治疗1周后MACE患者血清CK-MB、MyO、LDH水平均高于非MACE患者(P<0.05);入院时及治疗1周后血清CK-MB、MyO、LDH水平联合诊断的曲线下面积(Area Under the Curve,AUC)分别为0.752、0.794(P<0.05);入院时、治疗1周后血清CK-MB、MyO、LDH高水平患者发生MACE的危险度是低水平患者的数倍(P<0.05)。结论:血清CK-MB、MyO、LDH水平与慢性心力衰竭患者病情程度及预后相关,临床检测其水平对评估患者近期预后具有重要价值。
