为了解促天麻(Gastrodia elata)种子萌发真菌的物种多样性,发掘更多促天麻种子萌发真菌资源,笔者对我国亚热带地区广义小菇属(Mycena sensu lato)真菌开展广泛调查和采集,采集324份标本,组织分离获得401个菌株。基于形态特征和ITS、LSU...为了解促天麻(Gastrodia elata)种子萌发真菌的物种多样性,发掘更多促天麻种子萌发真菌资源,笔者对我国亚热带地区广义小菇属(Mycena sensu lato)真菌开展广泛调查和采集,采集324份标本,组织分离获得401个菌株。基于形态特征和ITS、LSU序列的初步分析,选取22个潜在功能菌株与天麻种子开展共萌发研究,结果表明:18个菌株可促进天麻种子萌发,通过进一步形态观察和分子系统发育研究,18个菌株隶属于5属13种,雅典娜小菇属(Atheniella)1种、胶孔菌属(Favolaschia)1种、小菇属(Mycena)8种、扇菇属(Panellus)1种和黏柄小菇属(Roridomyces)2种;13个菌株能促使天麻种子萌发形成天麻原球茎并继续分化,4个菌株使天麻种子的萌发率超过90%。两个沟纹小菇(M. abramsii)菌株和1个红斑小菇(M. maculata)菌株的促天麻种子萌发率均高于目前广泛使用的3个商用黄缘小菇(M. citrinomarginata)菌株(SHXG、MFJ和TMMFJ)。展开更多
Four fungal species of Mycena were identificated from the strains isolated from the protocorm and roots of Orchidaceae species.M anoectochial was isolated from the Proocorm of G.elata, M orchidicola, M.dendrobii and M...Four fungal species of Mycena were identificated from the strains isolated from the protocorm and roots of Orchidaceae species.M anoectochial was isolated from the Proocorm of G.elata, M orchidicola, M.dendrobii and M anoectochila respectively separated from the roots of Cymbidium sinense, Dendrobium candidum and Anoectochilus roxburghii. The symbiotic germination test beteen G. Elata seeds and the above fungal species demonstrated that these flingal species can stimulate the seed germination, Which means the species of Mycena may be the key mycorrhizal fungi of G elata, because there were no reports about the other fungal species Which stimulate G.elata seed germination. Besides G. elata, the above species of Mycena also distributed the roots of the other orchids.展开更多
文摘Four fungal species of Mycena were identificated from the strains isolated from the protocorm and roots of Orchidaceae species.M anoectochial was isolated from the Proocorm of G.elata, M orchidicola, M.dendrobii and M anoectochila respectively separated from the roots of Cymbidium sinense, Dendrobium candidum and Anoectochilus roxburghii. The symbiotic germination test beteen G. Elata seeds and the above fungal species demonstrated that these flingal species can stimulate the seed germination, Which means the species of Mycena may be the key mycorrhizal fungi of G elata, because there were no reports about the other fungal species Which stimulate G.elata seed germination. Besides G. elata, the above species of Mycena also distributed the roots of the other orchids.
