The dissemination of insect-borne plant pathogens relies on their ability to influence vector behavior.Certain bacteria-infected plants exhibit increased attractiveness to vectors;however,the underlying mech-anisms re...The dissemination of insect-borne plant pathogens relies on their ability to influence vector behavior.Certain bacteria-infected plants exhibit increased attractiveness to vectors;however,the underlying mech-anisms remain poorly characterized.Huanglongbing(HLB),a devastating citrus disease,is primarily caused by the bacterium"Candidatus Liberibacter asiaticus"(CLas)and transmitted by psyllid vectors.In this study,we demonstrate that the effector protein SDE5,secreted by CLas,suppresses the biosyn-thesis of volatile terpenoids in host citrus plants,thereby enhancing psyllid attraction.Biochemically,SDE5 functions as an inhibitor of bacterial C-type lysozyme,facilitating both CLas infection and psyllid vec-tor attraction.Two plant U-box(PUB)E3 ligases,PUB10 and PUB21,are recruited by SDE5 to promote ubiq-uitination and proteasomal degradation of MYC2,a key transcription factor in jasmonate signaling and terpene-based anti-herbivore defenses.Furthermore,SDE5 interferes with MYC2 dimerization,diminishing its ability to activate terpene biosynthesis genes.This dual suppression markedly reduces volatile terpe-noid emissions in SDE5-transgenic citrus lines,resulting in increased psyllid attraction and enhanced psyl-lid fitness.Conversely,the anti-proteolysis peptide 3-14(APP 3-14),which stabilizes the MYC2 protein and inhibits the HLB pathogen,enhances volatile terpenoid emission and repels psyllids.These findings pro-vide a novel strategy for disrupting mutualistic interactions between plant bacterial pathogens and insect vectors by modulation of olfactory defense.展开更多
Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an i...Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.展开更多
The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.Howev...The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.However,the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown.In this study,two AP2/ERF transcription factors(TFs),CaERF102 and CaERF111,were characterized for their role in the capsaicinoid biosynthesis process.Expression analysis of two ERFs and capsaicinoid biosynthetic genes(CBGs)suggested that they were associated with capsaicinoid biosynthesis.Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs.The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs,and this activation was significantly enhanced when CaMYC2 was co-expressed.Moreover,CaERF102 and CaERF111 were found to interact with CaMYC2.This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.展开更多
基金supported by the China National Key Research and Development Program(2023YFC2606800,2021YFD1400800,and 2024YFC3406000)the National Natural Science Foundation of China(32125032)+2 种基金the Inner Mongolia Science and Technology Program(2023KJHZ0018)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0810000 and XDA0450000)Project of the State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine.
文摘The dissemination of insect-borne plant pathogens relies on their ability to influence vector behavior.Certain bacteria-infected plants exhibit increased attractiveness to vectors;however,the underlying mech-anisms remain poorly characterized.Huanglongbing(HLB),a devastating citrus disease,is primarily caused by the bacterium"Candidatus Liberibacter asiaticus"(CLas)and transmitted by psyllid vectors.In this study,we demonstrate that the effector protein SDE5,secreted by CLas,suppresses the biosyn-thesis of volatile terpenoids in host citrus plants,thereby enhancing psyllid attraction.Biochemically,SDE5 functions as an inhibitor of bacterial C-type lysozyme,facilitating both CLas infection and psyllid vec-tor attraction.Two plant U-box(PUB)E3 ligases,PUB10 and PUB21,are recruited by SDE5 to promote ubiq-uitination and proteasomal degradation of MYC2,a key transcription factor in jasmonate signaling and terpene-based anti-herbivore defenses.Furthermore,SDE5 interferes with MYC2 dimerization,diminishing its ability to activate terpene biosynthesis genes.This dual suppression markedly reduces volatile terpe-noid emissions in SDE5-transgenic citrus lines,resulting in increased psyllid attraction and enhanced psyl-lid fitness.Conversely,the anti-proteolysis peptide 3-14(APP 3-14),which stabilizes the MYC2 protein and inhibits the HLB pathogen,enhances volatile terpenoid emission and repels psyllids.These findings pro-vide a novel strategy for disrupting mutualistic interactions between plant bacterial pathogens and insect vectors by modulation of olfactory defense.
基金supported by the National Natural Science Foundation of China(Grant Nos.32202542 and U20A2045)the Project of Major Science and Technology in Anhui Province(Grant No.202003a06020021)+2 种基金the Project of Science and Technology of Yunnan Province(Grant No.202102AE090038)Anhui Provincial Natural Science Foundation(Grant No.2108085QC121)the Natural Science Projects for Colleges and Universities in the Anhui Province(Grant No.KJ2021A0145)。
文摘Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.
基金funded by the National Natural Science Foundation of China(Grant Nos.32202502,U21A20230,32070331,32102380 and 32072580)National Key Research and Development Program(Grant No.2018YFD1000800)+3 种基金the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202080001)the Special Fund for Seed Industry of Guangdong Province Rural Revitalization Strategy(Grant No.2022-NPY00-024)Tibet Autonomous Region of Lhasa City Science and Technology Project(Grant No.LSKJ202310)the Science and Technology Project of Bijie City(Grant No.BKK2022-3)。
文摘The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.However,the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown.In this study,two AP2/ERF transcription factors(TFs),CaERF102 and CaERF111,were characterized for their role in the capsaicinoid biosynthesis process.Expression analysis of two ERFs and capsaicinoid biosynthetic genes(CBGs)suggested that they were associated with capsaicinoid biosynthesis.Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs.The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs,and this activation was significantly enhanced when CaMYC2 was co-expressed.Moreover,CaERF102 and CaERF111 were found to interact with CaMYC2.This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.
