目的探讨Fox M1、Cep55及c-Myc蛋白在基底细胞样型乳腺癌(BLBC)中的表达及临床意义。方法采用免疫组化方法检测66例BLBC、70例NON-BLBC和66例癌旁正常乳腺组织中Fox M1、Cep55及c-Myc蛋白的表达情况及三者间的相互关系。结果 Fox M1蛋白...目的探讨Fox M1、Cep55及c-Myc蛋白在基底细胞样型乳腺癌(BLBC)中的表达及临床意义。方法采用免疫组化方法检测66例BLBC、70例NON-BLBC和66例癌旁正常乳腺组织中Fox M1、Cep55及c-Myc蛋白的表达情况及三者间的相互关系。结果 Fox M1蛋白在BLBC、NON-BLBC和癌旁正常乳腺组织中的阳性表达率分别为77.3%(51/66)、60.0%(42/70)、13.6%(9/66),Cep55蛋白在BLBC、NON-BLBC和癌旁正常乳腺组织中的阳性表达率分别为74.2%(49/66)、57.1%(40/70)、16.7%(11/66),c-Myc蛋白在BLBC、NON-BLBC和癌旁正常乳腺组织中的阳性表达率分别为71.2%(47/66)、54.3%(38/70)、22.7%(15/66),差异均有统计学意义(P<0.05)。Fox M1、Cep55及c-Myc蛋白的表达与BLBC的TNM分期及淋巴结转移情况密切相关(P<0.05),而与年龄、绝经与否、肿块大小无关(P>0.05)。Fox M1和Cep55蛋白在BLBC中的表达呈正相关关系(P<0.05),Fox M1和c-Myc蛋白在BLBC中的表达呈正相关关系(P<0.05),而Cep55与c-Myc的表达没有相关性(P>0.05)。结论 Fox M1、Cep55及c-Myc蛋白可能参与了BLBC的发生、发展,并且Fox M1分别与Cep55及c-Myc在BLBC发生发展过程中可能有一定的协同作用;Cep55蛋白与c-Myc蛋白在BLBC中的表达没有相关性,说明Cep55及c-Myc可能通过不同的作用对BLBC的发生发展过程产生影响。展开更多
目的探讨CIP2A、c-myc在基底细胞样乳腺癌(basal-like breast carcinoma,BLBC)、非基底细胞样乳腺癌(non-basal-likebreast cancer,NON-BLBC)及正常乳腺组织中的表达及其之间的相互关系。方法采用免疫组化MaxVision两步法对43例BLBC、53...目的探讨CIP2A、c-myc在基底细胞样乳腺癌(basal-like breast carcinoma,BLBC)、非基底细胞样乳腺癌(non-basal-likebreast cancer,NON-BLBC)及正常乳腺组织中的表达及其之间的相互关系。方法采用免疫组化MaxVision两步法对43例BLBC、53例NON-BLBC及20例正常乳腺组织中CIP2A、c-myc的表达进行检测。结果 CIP2A、c-myc蛋白在BLBC中的表达明显高于正常乳腺组织,且与BLBC淋巴结转移及临床分期有关,差异均有统计学意义(P<0.05)。CIP2A与c-myc蛋白的表达呈正相关(r=0.446,P<0.01)。结论 CIP2A可能与BLBC的发生、发展相关,且进一步支持CIP2A与c-myc之间的正反馈调节作用。展开更多
Background: Dose-modified (dm) CODOX-M/IVAC is commonly used for Burkitt lymphoma (BL) and B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (BCL-U;previo...Background: Dose-modified (dm) CODOX-M/IVAC is commonly used for Burkitt lymphoma (BL) and B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (BCL-U;previously Burkitt-like lymphoma-BLL). Methods: We evaluated the clinical characteristics, outcomes and prognostic markers in patients treated with dmCODOX-M/IVAC+/- rituximab (R) at a single academic center. Results: 31 patients with BL (n = 21) or BCL-U (n = 10) were included. The median age was 45, and 90% were high risk. The 2-year overall survival (OS) and progression-free survival (PFS) were 69% and 62%, respectively, with no differences between BL and BCL-U. By multivariable analysis, rituximab use was significantly associated with improved OS (Hazard Ratio 0.15;95% CI 0.04 - 0.56) while elevated LDH (Hazard Ratio 2.84, 95% CI 1.52 - 5.29) and rituximab use (Hazard Ratio 0.054, 95% CI 0.01 - 0.32) predicted PFS. Conclusion: DmCODOX-M/IVAC+/- R chemotherapy provides equivalent survival for both BL and BCL-U patients. The addition of rituximab improves overall and progression free survival and is recommended.展开更多
Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) ...Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of Wx.Here, we determined that the GLYCOGEN SYNTHASE KINASE 5(OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites(Thr-28,Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimicvariantOsEBP89E–OsBP5but relatively strong when regulated by the nonphosphorylatable variant OsEBP89A–OsBP5.Therefore, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing.展开更多
文摘Background: Dose-modified (dm) CODOX-M/IVAC is commonly used for Burkitt lymphoma (BL) and B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (BCL-U;previously Burkitt-like lymphoma-BLL). Methods: We evaluated the clinical characteristics, outcomes and prognostic markers in patients treated with dmCODOX-M/IVAC+/- rituximab (R) at a single academic center. Results: 31 patients with BL (n = 21) or BCL-U (n = 10) were included. The median age was 45, and 90% were high risk. The 2-year overall survival (OS) and progression-free survival (PFS) were 69% and 62%, respectively, with no differences between BL and BCL-U. By multivariable analysis, rituximab use was significantly associated with improved OS (Hazard Ratio 0.15;95% CI 0.04 - 0.56) while elevated LDH (Hazard Ratio 2.84, 95% CI 1.52 - 5.29) and rituximab use (Hazard Ratio 0.054, 95% CI 0.01 - 0.32) predicted PFS. Conclusion: DmCODOX-M/IVAC+/- R chemotherapy provides equivalent survival for both BL and BCL-U patients. The addition of rituximab improves overall and progression free survival and is recommended.
基金financially supported by the Innovation Program of Shanghai Municipal Education Commission (2023ZKZD05)the National Natural Science Foundation of China (31971918, 32172043)+1 种基金the Agriculture Research System of Shanghai, China (Grant No. 202203)the Shanghai Science and Technology Innovation Action Plan Project (22N11900200)。
文摘Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of Wx.Here, we determined that the GLYCOGEN SYNTHASE KINASE 5(OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites(Thr-28,Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimicvariantOsEBP89E–OsBP5but relatively strong when regulated by the nonphosphorylatable variant OsEBP89A–OsBP5.Therefore, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing.
