Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties.While light is a well-known regulator of anthocyanin biosynthesis,the molecular basis of light-independent a...Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties.While light is a well-known regulator of anthocyanin biosynthesis,the molecular basis of light-independent anthocyanin accumulation remains underexplored.In this study,integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry(Vaccinium corymbosum‘Bluetta’)fruit was slightly decreased by light-impermeable bagging treatment,while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels,suggesting a slight influence of the bagging treatment on anthocyanin accumulation.Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content.Noticeably,two VcMYBA/MYB1s were not transcriptionally altered by the light-impermeable bagging treatment.Consistently,histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of VcMYBA/MYB1.Moreover,WGCNA analysis revealed 3759 genes positively associated with MYBA/MYB1 such as ethylene-associated genes,etc.Additionally,VcbZIP55s and VcCOP1s were activated and inactivated by the bagging treatment,respectively.These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit.展开更多
基金funded by the National Key R&D Program of China(Grant No.2022YFD1600500)the National Natural Science Foundation of China(Grant No.32272680).
文摘Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties.While light is a well-known regulator of anthocyanin biosynthesis,the molecular basis of light-independent anthocyanin accumulation remains underexplored.In this study,integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry(Vaccinium corymbosum‘Bluetta’)fruit was slightly decreased by light-impermeable bagging treatment,while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels,suggesting a slight influence of the bagging treatment on anthocyanin accumulation.Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content.Noticeably,two VcMYBA/MYB1s were not transcriptionally altered by the light-impermeable bagging treatment.Consistently,histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of VcMYBA/MYB1.Moreover,WGCNA analysis revealed 3759 genes positively associated with MYBA/MYB1 such as ethylene-associated genes,etc.Additionally,VcbZIP55s and VcCOP1s were activated and inactivated by the bagging treatment,respectively.These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit.
