Lodging reduces grain yield and quality in cereal crops.Lodging resistance is affected by the strength of the culm,which is influenced by the culm diameter,culm wall thickness,and cell wall composition.To explore the ...Lodging reduces grain yield and quality in cereal crops.Lodging resistance is affected by the strength of the culm,which is influenced by the culm diameter,culm wall thickness,and cell wall composition.To explore the genetic architecture of culm diameter in rice(Oryza sativa),we conducted a genome-wide association study(GWAS).We identified STRONG CULM 2(STRONG2),which encodes the mannan synthase CSLA5,and showed that plants that overexpressed this gene had increased culm diameter and improved lodging resistance.STRONG2 appears to increase the levels of cell wall components,such as mannose and cellulose,thereby enhancing sclerenchyma development in stems.SNP14931253 in the STRONG2 promoter contributes to variation in STRONG2 expression in natural germplasms and the transcription factor MYB61 directly activates STRONG2 expression.Furthermore,STRONG2 overexpressing plants produced significantly more grains per panicle and heavier grains than the wild-type plants.These results demonstrate that the MYB61-STRONG2 module positively regulates culm diameter and lodging resistance,information that could guide breeding efforts for improved yield in rice.展开更多
基金funded by the Biological Breeding-National Science and Technology Major Project(2023ZD0406803)the Agro ST Project(NK2022050103)+6 种基金the National Natural Science Foundation of China(32272123 and 32072036)the Hainan Provincial Natural Science Foundation of China(324QN358)the Chinese Universities Scientific Fund(2024TC189)the Postdoctoral Research Projects in Hainan Province(343812)the Hainan Seed Industry Laboratory and the China National Seed Group(B23YQ1517 and B23CQ15HP)the Special Plan for Key Laboratory of“Western Light Western cross team”(xbzg-zdsys202111)the 2115 Talent Development Program of China Agricultural University。
文摘Lodging reduces grain yield and quality in cereal crops.Lodging resistance is affected by the strength of the culm,which is influenced by the culm diameter,culm wall thickness,and cell wall composition.To explore the genetic architecture of culm diameter in rice(Oryza sativa),we conducted a genome-wide association study(GWAS).We identified STRONG CULM 2(STRONG2),which encodes the mannan synthase CSLA5,and showed that plants that overexpressed this gene had increased culm diameter and improved lodging resistance.STRONG2 appears to increase the levels of cell wall components,such as mannose and cellulose,thereby enhancing sclerenchyma development in stems.SNP14931253 in the STRONG2 promoter contributes to variation in STRONG2 expression in natural germplasms and the transcription factor MYB61 directly activates STRONG2 expression.Furthermore,STRONG2 overexpressing plants produced significantly more grains per panicle and heavier grains than the wild-type plants.These results demonstrate that the MYB61-STRONG2 module positively regulates culm diameter and lodging resistance,information that could guide breeding efforts for improved yield in rice.
