Plants mount induced resistance and adult-plant resistance against different pathogens throughout the whole growth period.Rice production faces threats from multiple major diseases,including rice blast,sheath blight,a...Plants mount induced resistance and adult-plant resistance against different pathogens throughout the whole growth period.Rice production faces threats from multiple major diseases,including rice blast,sheath blight,and bacterial leaf blight.Here,we report that the miR172a–SNB–MYB30 module regulates both induced and adult-plant resistance to these three major diseases via lignification in rice.Mechanistically,pathogen infections induce the expression of miR172a,which downregulates the transcription factor SNB to release its suppression of MYB30,leading to an increase in lignin biosynthesis and disease resistance throughout the whole growth period.Moreover,expression levels of miR172a and MYB30 gradually increase and are consistently correlated with lignin contents and disease resistance during rice development,reaching a peak at full maturity,whereas SNB RNA levels are negatively correlated with lignin contents and disease resistance,indicating the involvement of the miR172a–SNB–MYB30 module in adult-plant resistance.The functional domain of SNB protein and its binding sites in the MYB30 promoter are highly conserved among more than 4000 rice accessions,while abnormal expression of miR172a,SNB,or MYB30 compromises yield traits,suggesting artificial selection of the miR172a–SNB–MYB30 module during rice domestication.Taken together,these results reveal a novel role for a conserved miRNA-regulated module that contributes significantly to induced and adult-plant resistance against multiple pathogens by increasing lignin accumulation,deepening our understanding of broad-spectrum resistance and adult-plant resistance.展开更多
Anthocyanins play diverse roles in plant physiology and stress adaptation.In Arabidopsis,the MYB–bHLH–WD40(MBW)complex has a crucial role in the regulation of anthocyanin synthesis.Here,we report that the R2R3-MYB t...Anthocyanins play diverse roles in plant physiology and stress adaptation.In Arabidopsis,the MYB–bHLH–WD40(MBW)complex has a crucial role in the regulation of anthocyanin synthesis.Here,we report that the R2R3-MYB transcription factor MYB30 and the ubiquitin E3 ligase RHA2b participate in anthocyanin biosynthesis through regulation of the MBW complex.MYB30 was found to negatively regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis seedlings.Expression of multiple genes involved inflavo-noid or anthocyanin biosynthesis was affected in the myb30 mutant,and MYB30 directly repressed the expression of MYB75,which encodes a core component of the MBW complex,by binding to its promoter.Moreover,MYB30 physically interacted with MYB75 to inhibit its activity by repressing MBW complex as-sembly.In addition,sucrose treatment signicantly promoted MYB30 degradation via the action of RHA2b.The ubiquitination and degradation of MYB30 were signicantly attenuated in the rha2b mutant un-der high-sucrose treatment,and further analysis showed that MYB75 directly promoted RHA2b expression in response to high sucrose.Our work thus reveals an anthocyanin biosynthetic regulatory module,RHA2b–MYB30,that controls the function of the MBW complex via MYB75.The repression of MYB75 by MYB30 is released by MYB75-induced RHA2b expression,thus ensuring the self-activation of MYB75 when anthocy-anin synthesis is needed.展开更多
The transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)plays a crucial role in abscisic acid(ABA)signaling during seed germination.However,how ABI5 is regulated during this process is poorly understood.Here,we repor...The transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)plays a crucial role in abscisic acid(ABA)signaling during seed germination.However,how ABI5 is regulated during this process is poorly understood.Here,we report that the ubiquitin E3 ligase MIEL1 and its target transcription factor MYB30 modulate ABA responses in Arabidopsis thaliana during seed germination and seedling establishment via the precise regulation of ABI5.MIEL1 interacts with and ubiquitinates ABI5 to facilitate its degradation during germination.The transcription factor MYB30,whose turnover is mediated by MIEL1 during seed germination,also interacts with ABI5 to interfere with its transcriptional activity.MYB30 functions downstream of MIEL1 in the ABA response,and both are epistatic to ABI5 in ABA-mediated inhibition of seed germination and postgerminative growth.ABA treatment induces the degradation of MIEL1 and represses the interaction between MIEL1 and ABI5/MYB30,thus releasing both ABI5 and MYB30.Our results demonstrate that MIEL1 directly mediates the proteasomal degradation of ABI5 and inhibits its activity via the release of its target protein MYB30,thus ensuring precise ABA signaling during seed germination and seedling establishment.展开更多
基金supported by the National Natural Science Foundation of China(nos.32121003,32401845,32372553,and 32172417)the Natural Science Foundation of Sichuan Province(2022JDTD0023 and 2022NSFSC0174)the Open Research Fund of State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKLZD202202,SKL-ZY202202,SKL-ZY202205,and SKL-ZY202209).
文摘Plants mount induced resistance and adult-plant resistance against different pathogens throughout the whole growth period.Rice production faces threats from multiple major diseases,including rice blast,sheath blight,and bacterial leaf blight.Here,we report that the miR172a–SNB–MYB30 module regulates both induced and adult-plant resistance to these three major diseases via lignification in rice.Mechanistically,pathogen infections induce the expression of miR172a,which downregulates the transcription factor SNB to release its suppression of MYB30,leading to an increase in lignin biosynthesis and disease resistance throughout the whole growth period.Moreover,expression levels of miR172a and MYB30 gradually increase and are consistently correlated with lignin contents and disease resistance during rice development,reaching a peak at full maturity,whereas SNB RNA levels are negatively correlated with lignin contents and disease resistance,indicating the involvement of the miR172a–SNB–MYB30 module in adult-plant resistance.The functional domain of SNB protein and its binding sites in the MYB30 promoter are highly conserved among more than 4000 rice accessions,while abnormal expression of miR172a,SNB,or MYB30 compromises yield traits,suggesting artificial selection of the miR172a–SNB–MYB30 module during rice domestication.Taken together,these results reveal a novel role for a conserved miRNA-regulated module that contributes significantly to induced and adult-plant resistance against multiple pathogens by increasing lignin accumulation,deepening our understanding of broad-spectrum resistance and adult-plant resistance.
基金supported by the National Natural Science Foundation of China (grants 32170295 and 31870241 to H.Z.)the China Postdoctoral Science Foundation (grant 2023M730776 to J.H.)the Institutional Research Fund of Sichuan University (grant 2020SCUNL212 to H.L.).
文摘Anthocyanins play diverse roles in plant physiology and stress adaptation.In Arabidopsis,the MYB–bHLH–WD40(MBW)complex has a crucial role in the regulation of anthocyanin synthesis.Here,we report that the R2R3-MYB transcription factor MYB30 and the ubiquitin E3 ligase RHA2b participate in anthocyanin biosynthesis through regulation of the MBW complex.MYB30 was found to negatively regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis seedlings.Expression of multiple genes involved inflavo-noid or anthocyanin biosynthesis was affected in the myb30 mutant,and MYB30 directly repressed the expression of MYB75,which encodes a core component of the MBW complex,by binding to its promoter.Moreover,MYB30 physically interacted with MYB75 to inhibit its activity by repressing MBW complex as-sembly.In addition,sucrose treatment signicantly promoted MYB30 degradation via the action of RHA2b.The ubiquitination and degradation of MYB30 were signicantly attenuated in the rha2b mutant un-der high-sucrose treatment,and further analysis showed that MYB75 directly promoted RHA2b expression in response to high sucrose.Our work thus reveals an anthocyanin biosynthetic regulatory module,RHA2b–MYB30,that controls the function of the MBW complex via MYB75.The repression of MYB75 by MYB30 is released by MYB75-induced RHA2b expression,thus ensuring the self-activation of MYB75 when anthocy-anin synthesis is needed.
基金supported by the National Natural Science Foundation of China(31872656,32170295,and 31870241)the Natural Science Foundation of Henan(212300410022)the project“the Program for Innovative Research Team(in Science and Technology)in University of Henan Province”(21IRTSTHN019)。
文摘The transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)plays a crucial role in abscisic acid(ABA)signaling during seed germination.However,how ABI5 is regulated during this process is poorly understood.Here,we report that the ubiquitin E3 ligase MIEL1 and its target transcription factor MYB30 modulate ABA responses in Arabidopsis thaliana during seed germination and seedling establishment via the precise regulation of ABI5.MIEL1 interacts with and ubiquitinates ABI5 to facilitate its degradation during germination.The transcription factor MYB30,whose turnover is mediated by MIEL1 during seed germination,also interacts with ABI5 to interfere with its transcriptional activity.MYB30 functions downstream of MIEL1 in the ABA response,and both are epistatic to ABI5 in ABA-mediated inhibition of seed germination and postgerminative growth.ABA treatment induces the degradation of MIEL1 and represses the interaction between MIEL1 and ABI5/MYB30,thus releasing both ABI5 and MYB30.Our results demonstrate that MIEL1 directly mediates the proteasomal degradation of ABI5 and inhibits its activity via the release of its target protein MYB30,thus ensuring precise ABA signaling during seed germination and seedling establishment.
