Some cultivars of pear(Pyrus L.)show attractive red fruit skin due to anthocyanin accumulation.This pigmentation can be affected by environmental conditions,especially light.To explore the light-induced regulation net...Some cultivars of pear(Pyrus L.)show attractive red fruit skin due to anthocyanin accumulation.This pigmentation can be affected by environmental conditions,especially light.To explore the light-induced regulation network for anthocyanin biosynthesis and fruit coloration in pear,small RNA libraries and mRNA libraries from fruit skins of‘Yunhongyihao’pear were constructed to compare the difference between bagging and debagging treatments.Analysis of RNA-seq of fruit skins with limited light(bagged)and exposed to light(debagged),showed that PyPIF5 was down-regulated after bag removal.PymiR156a was also differentially expressed between bagged and debagged fruit skins.We found that PyPIF5 negatively regulated PymiR156a expression in bagged fruits by directly binding to the G-box motif in its promoter.In addition,PymiR156a overexpression promoted anthocyanin accumulation in both pear skin and apple calli.We confirmed that PymiR156a mediated the cleavage of PySPL9,and that the target PySPL9 protein could form heterodimers with two key anthocyanin regulators(PyMYB114/PyMYB10).We proposed a new module of PyPIF5-PymiR156a-PySPL9-PyMYB114/MYB10.When the bagged fruits were re-exposed to light,PyPIF5 was down-regulated and its inhibitory effect on PymiR156a was weakened,which leads to degradation of the target PySPL,thus eliminating the blocking effect of PySPL on the formation of the regulatory MYB complexes.Ultimately,this promotes anthocyanin biosynthesis in pear skin.展开更多
Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economica...Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economically important in eliminating poverty and protecting ecology in the Yangtze River Three Gorges Reservoir.However,rain-induced cracking(rain-cracking,literally skin cracking caused by rain)is a limitation to‘Wushancuili'fruit production and causes severe losses.This study reported a high-quality‘Wushancuili'genome assembly consisting of a 302.17-Mb sequence with eight pseudo-chromosomes and a contig N50 of 23.59 Mb through the combination of Illumina sequencing,Pacific Biosciences HiFiⅢsequencing,and high-throughput chromosome conformation capture technology.A total of 25109 protein-coding genes are predicted and 54.17%of the genome is composed of repetitive sequences.‘Wushancuili'underwent a remarkable orthoselection during evolution.Gene identification revealed that loss-of-function in four core MYB10 genes results in the anthocyanin deficiency and absence of red color,revealing the green coloration due to the residual high chlorophyll in fruit skin.Besides,the occurrence of cracking is assumed to be closely associated with cell wall modification and frequently rain-induced pathogen enrichment through transcriptomic analysis.The loss of MYB10 genes might render fruit more susceptible to pathogen-mediated cracking by weakening the epidermal strength and reactive oxygen species(ROS)scavenging.Our findings provided fundamental knowledge regarding fruit coloration and rain-cracking and will facilitate genetic improvement and cultivation management in Chinese plums.展开更多
基金This work was supported by the National Natural Science Foundation of China(31820103012)the National Key Research and Development Program(2018YFD1000200)China Agriculture Research System of MOF and MARA,the Earmarked Fund for Jiangsu Agricultural Industry Technology System(JATS[2020]401).
文摘Some cultivars of pear(Pyrus L.)show attractive red fruit skin due to anthocyanin accumulation.This pigmentation can be affected by environmental conditions,especially light.To explore the light-induced regulation network for anthocyanin biosynthesis and fruit coloration in pear,small RNA libraries and mRNA libraries from fruit skins of‘Yunhongyihao’pear were constructed to compare the difference between bagging and debagging treatments.Analysis of RNA-seq of fruit skins with limited light(bagged)and exposed to light(debagged),showed that PyPIF5 was down-regulated after bag removal.PymiR156a was also differentially expressed between bagged and debagged fruit skins.We found that PyPIF5 negatively regulated PymiR156a expression in bagged fruits by directly binding to the G-box motif in its promoter.In addition,PymiR156a overexpression promoted anthocyanin accumulation in both pear skin and apple calli.We confirmed that PymiR156a mediated the cleavage of PySPL9,and that the target PySPL9 protein could form heterodimers with two key anthocyanin regulators(PyMYB114/PyMYB10).We proposed a new module of PyPIF5-PymiR156a-PySPL9-PyMYB114/MYB10.When the bagged fruits were re-exposed to light,PyPIF5 was down-regulated and its inhibitory effect on PymiR156a was weakened,which leads to degradation of the target PySPL,thus eliminating the blocking effect of PySPL on the formation of the regulatory MYB complexes.Ultimately,this promotes anthocyanin biosynthesis in pear skin.
基金financially supported by the Construction Program for Chongqing's Distinctive“Wushancuili”Industry(Grant No.4322200370)Strategic Cooperation Project of Chongqing Municipality and Chinese Academy of Agricultural Sciences(Grant No.4322300181)Fundamental Research Funds for Central Universities-Talent induction project(Grant Nos.SWU-KR22001,SWU-KQ22070)。
文摘Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economically important in eliminating poverty and protecting ecology in the Yangtze River Three Gorges Reservoir.However,rain-induced cracking(rain-cracking,literally skin cracking caused by rain)is a limitation to‘Wushancuili'fruit production and causes severe losses.This study reported a high-quality‘Wushancuili'genome assembly consisting of a 302.17-Mb sequence with eight pseudo-chromosomes and a contig N50 of 23.59 Mb through the combination of Illumina sequencing,Pacific Biosciences HiFiⅢsequencing,and high-throughput chromosome conformation capture technology.A total of 25109 protein-coding genes are predicted and 54.17%of the genome is composed of repetitive sequences.‘Wushancuili'underwent a remarkable orthoselection during evolution.Gene identification revealed that loss-of-function in four core MYB10 genes results in the anthocyanin deficiency and absence of red color,revealing the green coloration due to the residual high chlorophyll in fruit skin.Besides,the occurrence of cracking is assumed to be closely associated with cell wall modification and frequently rain-induced pathogen enrichment through transcriptomic analysis.The loss of MYB10 genes might render fruit more susceptible to pathogen-mediated cracking by weakening the epidermal strength and reactive oxygen species(ROS)scavenging.Our findings provided fundamental knowledge regarding fruit coloration and rain-cracking and will facilitate genetic improvement and cultivation management in Chinese plums.
