MYB transcription factors are widely involved in the development of and physiological processes in plants.Here,we isolated the chrysanthemum R2R3-MYB family transcription factor CmMYB15,a homologous gene of AtMYB15.It...MYB transcription factors are widely involved in the development of and physiological processes in plants.Here,we isolated the chrysanthemum R2R3-MYB family transcription factor CmMYB15,a homologous gene of AtMYB15.It was demonstrated that CmMYB15 expression was induced by aphids and that CmMYB15 could bind to AC elements,which usually exist in the promoter of lignin biosynthesis genes.Overexpression of CmMYB15 in chrysanthemum enhanced the resistance of aphids.Additionally,the content of lignin and the expression of several lignin biosynthesis genes increased.In summary,the results indicate that CmMYB15 regulates lignin biosynthesis genes that enhance the resistance of chrysanthemum to aphids.展开更多
Fruit lycopene,shape,and resistance are essential traits in vegetables whose final product is fruit,and they are also closely related to and strictly regulated by multiple transcription factors.Lycopene,which cannot b...Fruit lycopene,shape,and resistance are essential traits in vegetables whose final product is fruit,and they are also closely related to and strictly regulated by multiple transcription factors.Lycopene,which cannot be synthesized by the human body and can only be ingested from the outside,was important in maintaining human health.During fruit ripening and post-harvest,tomato plants face a variety of biotic or abiotic stresses,which might inf lict great damage to fruit quality due to its f lat shape and pointed tip during storage and transportation.Therefore,there is an urgent need for key molecular switches to simultaneously improve fruit lycopene and resistance to biotic stress during ripening.Here,we identified the MYB transcription factor SlMYB1 in tomato plants which could bind to the promoters of lycopene synthesis-related genes,SlLCY1,SlPSY2,and the pathogen-related gene SlPR5 directly,to regulate the fruit lycopene and resistance to Botrytis cinerea in tomato.In addition to regulating lycopene synthesis,SlMYB1 also regulates the content of soluble sugar,soluble protein and f lavonoid in tomato.What’s more,SlMYB1 could regulate the tomato fruit shape,making it smoother or f latter to prevent skin damage caused by vibration on fruits.RNA sequencing(RNA-seq)further showed that SlMYB1 fruit-specific expression lines had multiple differentially expressed genes compared with those from wild-type plants,suggesting that SlMYB1 might have multiple roles in fruit nutritional quality control and resistance to stresses,which is a rare occurrence in previous studies.In summary,our results revealed that SlMYB1 was an essential multi-functional transcription factor that could regulate the lycopene and resistance to Botrytis cinerea,and change the shape of fruit in tomato plants.展开更多
Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this...Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this study,we found that a key R2R3-MYB transcription factor,RcMYB1,plays a central role in rose anthocyanin biosynthesis.Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves.In 35S:RcMYB1 transgenic lines,a significant accumulation of anthocyanins occurred in leaves and petioles.We further identified two MBW complexes(RcMYB1-RcBHLH42-RcTTG1;RcMYB1-RcEGL1-RcTTG1)associated with anthocyanin accumulation.Yeast one-hybrid and luciferase assays showed that RcMYB1 could active its own gene promoter and those of other EBGs(early anthocyanin biosynthesis genes)and LBGs(late anthocyanin biosynthesis genes).In addition,both of the MBW complexes enhanced the transcriptional activity of RcMYB1 and LBGs.Interestingly,our results also indicate that RcMYB1 is involved in the metabolic regulation of carotenoids and volatile aroma.In summary,we found that RcMYB1 widely participates in the transcriptional regulation of ABGs(anthocyanin biosynthesis genes),indicative of its central role in the regulation of anthocyanin accumulation in rose.Our results provide a theoretical basis for the further improvement of the flower color trait in rose by breeding or genetic modification.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31672192)the Program for Key Research and Development,Jiangsu,China(Grant No.BE2017318)+1 种基金the National Key Research and Development Program of China(2018YFD1000402)the Fundamental Research Funds for the Central Universities(KJQN201812).
文摘MYB transcription factors are widely involved in the development of and physiological processes in plants.Here,we isolated the chrysanthemum R2R3-MYB family transcription factor CmMYB15,a homologous gene of AtMYB15.It was demonstrated that CmMYB15 expression was induced by aphids and that CmMYB15 could bind to AC elements,which usually exist in the promoter of lignin biosynthesis genes.Overexpression of CmMYB15 in chrysanthemum enhanced the resistance of aphids.Additionally,the content of lignin and the expression of several lignin biosynthesis genes increased.In summary,the results indicate that CmMYB15 regulates lignin biosynthesis genes that enhance the resistance of chrysanthemum to aphids.
基金supported by the National Natural Science Foundation(32072500,31872925,32272557)Shandong Province Key Research and Development Plan(2022TZXD0025,2021TZXD007-04-4)+1 种基金Taishan Scholar Program of Shandong Province,Major Basic Research Project of Natural Science Foundation of Shandong Province(ZR2022ZD23)Shandong Modern Agricultural Industry Technology System(SDAIT-04-08).
文摘Fruit lycopene,shape,and resistance are essential traits in vegetables whose final product is fruit,and they are also closely related to and strictly regulated by multiple transcription factors.Lycopene,which cannot be synthesized by the human body and can only be ingested from the outside,was important in maintaining human health.During fruit ripening and post-harvest,tomato plants face a variety of biotic or abiotic stresses,which might inf lict great damage to fruit quality due to its f lat shape and pointed tip during storage and transportation.Therefore,there is an urgent need for key molecular switches to simultaneously improve fruit lycopene and resistance to biotic stress during ripening.Here,we identified the MYB transcription factor SlMYB1 in tomato plants which could bind to the promoters of lycopene synthesis-related genes,SlLCY1,SlPSY2,and the pathogen-related gene SlPR5 directly,to regulate the fruit lycopene and resistance to Botrytis cinerea in tomato.In addition to regulating lycopene synthesis,SlMYB1 also regulates the content of soluble sugar,soluble protein and f lavonoid in tomato.What’s more,SlMYB1 could regulate the tomato fruit shape,making it smoother or f latter to prevent skin damage caused by vibration on fruits.RNA sequencing(RNA-seq)further showed that SlMYB1 fruit-specific expression lines had multiple differentially expressed genes compared with those from wild-type plants,suggesting that SlMYB1 might have multiple roles in fruit nutritional quality control and resistance to stresses,which is a rare occurrence in previous studies.In summary,our results revealed that SlMYB1 was an essential multi-functional transcription factor that could regulate the lycopene and resistance to Botrytis cinerea,and change the shape of fruit in tomato plants.
基金This work was supported by Shanghai Special Project of Capacity Construction for Local Colleges and Universities,No.20070502500Shanghai Science and Technology Agriculture Program,No.2022-02-08-00-12-F01146+1 种基金Science and Technology Commission of Shanghai Municipality,No.18DZ2260500Shanghai Plant Germplasm Resources Engineering Research Center,17DZ2252700.
文摘Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this study,we found that a key R2R3-MYB transcription factor,RcMYB1,plays a central role in rose anthocyanin biosynthesis.Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves.In 35S:RcMYB1 transgenic lines,a significant accumulation of anthocyanins occurred in leaves and petioles.We further identified two MBW complexes(RcMYB1-RcBHLH42-RcTTG1;RcMYB1-RcEGL1-RcTTG1)associated with anthocyanin accumulation.Yeast one-hybrid and luciferase assays showed that RcMYB1 could active its own gene promoter and those of other EBGs(early anthocyanin biosynthesis genes)and LBGs(late anthocyanin biosynthesis genes).In addition,both of the MBW complexes enhanced the transcriptional activity of RcMYB1 and LBGs.Interestingly,our results also indicate that RcMYB1 is involved in the metabolic regulation of carotenoids and volatile aroma.In summary,we found that RcMYB1 widely participates in the transcriptional regulation of ABGs(anthocyanin biosynthesis genes),indicative of its central role in the regulation of anthocyanin accumulation in rose.Our results provide a theoretical basis for the further improvement of the flower color trait in rose by breeding or genetic modification.
