BACKGROUND Esophageal squamous cell carcinoma(ESCC)is one of the most common malignant tumors globally,with its incidence particularly high in East Asia.AIM To analyze the expression of the stem cell marker musashi-1 ...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is one of the most common malignant tumors globally,with its incidence particularly high in East Asia.AIM To analyze the expression of the stem cell marker musashi-1 in patients with resectable ESCC undergoing neoadjuvant chemotherapy and its relationship with patient survival prognosis.METHODS A retrospective analysis was conducted on the clinical data of 74 ESCC patients treated at our hospital from June 2020 to January 2022.All patients received neoadjuvant chemotherapy and surgical resection.Immunohistochemistry(IHC)was used to detect musashi-1 expression in tumor tissues.Based on the expression intensity,patients were divided into group A(n=30,IHC total score>2 indicating high expression)and group B(n=44,IHC total score 0-2 indicating low expression).The clinical pathological differences between groups A and B were compared.The treatment outcomes of both groups were compared.Univariate and multivariate Cox regression analysis was performed to identify factors affecting patient prognosis.Kaplan-Meier survival analysis was used,and logrank tests were conducted to compare differences between groups.RESULTS There were statistically significant differences in tumor maximum diameter,T stage,N stage,clinical stage,pathological grade,lymphovascular invasion,and intraoperative blood loss between groups A and B(P<0.05).The disease control rate in group A(86.67%)was lower than that in group B(100.00%)(χ^(2)=3.868,P=0.049);the objective response rate in group A(33.33%)was lower than that in group B(70.45%)(χ^(2)=9.948,P=0.001).The proportion of tumor regression grade 3+4+5 grades in group A(80.00%)was higher than in group B(43.18%)(χ^(2)=9.933,P=0.001).Univariate analysis showed that tumor maximum diameter,T stage,N stage,clinical stage,pathological grade,and musashi-1 expression were associated with patient prognosis(P<0.05).Cox regression analysis model.The results indicated that T stage[hazard ratio(HR)=1.82,95%confidence interval(CI):2.14-7.37],N stage(HR=1.70,95%CI:1.12-2.36),clinical stage(HR=2.08,95%CI:1.36-3.85),pathological grade(HR=1.54,95%CI:1.07-2.41),and musashi-1 expression(HR=2.72,95%CI:2.03-4.11)were independent risk factors affecting patient prognosis(P<0.05).Kaplan-Meier survival curves showed that the median overall survival in group A was 17 months,while in group B it was 28 months.Log-rank analysis revealed that the overall survival rate in group A was worse than in group B(χ^(2)=2.635,P=0.033).CONCLUSION The expression of musashi-1 is closely related to the treatment efficacy,prognosis,and survival of ESCC patients.It is expected to be a potential biomarker for evaluating the efficacy and survival prognosis of ESCC patients.展开更多
AIM:To investigate expression of stem cell marker Musashi-1(Msi-1)in relationship to tumorigenesis and progression of intestinal-type gastric cancer(GC).METHODS:Endoscopic biopsy specimens and surgical specimens were ...AIM:To investigate expression of stem cell marker Musashi-1(Msi-1)in relationship to tumorigenesis and progression of intestinal-type gastric cancer(GC).METHODS:Endoscopic biopsy specimens and surgical specimens were obtained,including 54 cases of intestinal-type GC,41 high-grade intraepithelial neoplasia,57low-grade intraepithelial neoplasia,31 intestinal metaplasia,and 36 normal gastric mucosa.Specimens were fixed in 10%paraformaldehyde,conventionally dehydrated,embedded in paraffin,and sliced in 4-μm-thick serial sections.Two-step immunohistochemical staining was used to detect Msi-1 and proliferating cell nuclear antigen(PCNA)expression.Correlation analysis was conducted between Msi-1 and PCNA expression.The relationship between Msi-1 expression and clinicopathological parameters of GC was analyzed statistically.RESULTS:There were significant differences in Msi-1and PCNA expression in different pathological tissues(χ2=15.37,P<0.01;χ2=115.36,P<0.01).Msi-1and PCNA-positive cells were restricted to the isthmus of normal gastric glands.Expression levels of Msi-1and PCNA in intestinal metaplasia were significantly higher than in normal mucosa(U=392.0,P<0.05;U=40.50,P<0.01),whereas there was no significant difference compared to low or high-grade intraepithelial neoplasia.Msi-1 and PCNA expression in intestinaltype GC was higher than in high-grade intraepithelial neoplasia(U=798.0,P<0.05;U=688.0,P<0.01).There was a significantly positive correlation between Msi-1 and PCNA expression(rs=0.20,P<0.01).Msi-1expression in GC tissues was correlated with their lymph node metastasis and tumor node metastasis stage(χ2=12.62,P<0.01;χ2=11.24,P<0.05),but not with depth of invasion and the presence of distant metastasis.CONCLUSION:Msi-1-positive cells may play a key role in the early events of gastric carcinogenesis and may be involved in invasion and metastasis of GC.展开更多
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is one of the most common malignant tumors globally,with its incidence particularly high in East Asia.AIM To analyze the expression of the stem cell marker musashi-1 in patients with resectable ESCC undergoing neoadjuvant chemotherapy and its relationship with patient survival prognosis.METHODS A retrospective analysis was conducted on the clinical data of 74 ESCC patients treated at our hospital from June 2020 to January 2022.All patients received neoadjuvant chemotherapy and surgical resection.Immunohistochemistry(IHC)was used to detect musashi-1 expression in tumor tissues.Based on the expression intensity,patients were divided into group A(n=30,IHC total score>2 indicating high expression)and group B(n=44,IHC total score 0-2 indicating low expression).The clinical pathological differences between groups A and B were compared.The treatment outcomes of both groups were compared.Univariate and multivariate Cox regression analysis was performed to identify factors affecting patient prognosis.Kaplan-Meier survival analysis was used,and logrank tests were conducted to compare differences between groups.RESULTS There were statistically significant differences in tumor maximum diameter,T stage,N stage,clinical stage,pathological grade,lymphovascular invasion,and intraoperative blood loss between groups A and B(P<0.05).The disease control rate in group A(86.67%)was lower than that in group B(100.00%)(χ^(2)=3.868,P=0.049);the objective response rate in group A(33.33%)was lower than that in group B(70.45%)(χ^(2)=9.948,P=0.001).The proportion of tumor regression grade 3+4+5 grades in group A(80.00%)was higher than in group B(43.18%)(χ^(2)=9.933,P=0.001).Univariate analysis showed that tumor maximum diameter,T stage,N stage,clinical stage,pathological grade,and musashi-1 expression were associated with patient prognosis(P<0.05).Cox regression analysis model.The results indicated that T stage[hazard ratio(HR)=1.82,95%confidence interval(CI):2.14-7.37],N stage(HR=1.70,95%CI:1.12-2.36),clinical stage(HR=2.08,95%CI:1.36-3.85),pathological grade(HR=1.54,95%CI:1.07-2.41),and musashi-1 expression(HR=2.72,95%CI:2.03-4.11)were independent risk factors affecting patient prognosis(P<0.05).Kaplan-Meier survival curves showed that the median overall survival in group A was 17 months,while in group B it was 28 months.Log-rank analysis revealed that the overall survival rate in group A was worse than in group B(χ^(2)=2.635,P=0.033).CONCLUSION The expression of musashi-1 is closely related to the treatment efficacy,prognosis,and survival of ESCC patients.It is expected to be a potential biomarker for evaluating the efficacy and survival prognosis of ESCC patients.
基金Supported by Jinan Science and Technology Bureau for Independent Innovation Projects of Universities and Research Institutes in Jinan city,China,No.201102060
文摘AIM:To investigate expression of stem cell marker Musashi-1(Msi-1)in relationship to tumorigenesis and progression of intestinal-type gastric cancer(GC).METHODS:Endoscopic biopsy specimens and surgical specimens were obtained,including 54 cases of intestinal-type GC,41 high-grade intraepithelial neoplasia,57low-grade intraepithelial neoplasia,31 intestinal metaplasia,and 36 normal gastric mucosa.Specimens were fixed in 10%paraformaldehyde,conventionally dehydrated,embedded in paraffin,and sliced in 4-μm-thick serial sections.Two-step immunohistochemical staining was used to detect Msi-1 and proliferating cell nuclear antigen(PCNA)expression.Correlation analysis was conducted between Msi-1 and PCNA expression.The relationship between Msi-1 expression and clinicopathological parameters of GC was analyzed statistically.RESULTS:There were significant differences in Msi-1and PCNA expression in different pathological tissues(χ2=15.37,P<0.01;χ2=115.36,P<0.01).Msi-1and PCNA-positive cells were restricted to the isthmus of normal gastric glands.Expression levels of Msi-1and PCNA in intestinal metaplasia were significantly higher than in normal mucosa(U=392.0,P<0.05;U=40.50,P<0.01),whereas there was no significant difference compared to low or high-grade intraepithelial neoplasia.Msi-1 and PCNA expression in intestinaltype GC was higher than in high-grade intraepithelial neoplasia(U=798.0,P<0.05;U=688.0,P<0.01).There was a significantly positive correlation between Msi-1 and PCNA expression(rs=0.20,P<0.01).Msi-1expression in GC tissues was correlated with their lymph node metastasis and tumor node metastasis stage(χ2=12.62,P<0.01;χ2=11.24,P<0.05),but not with depth of invasion and the presence of distant metastasis.CONCLUSION:Msi-1-positive cells may play a key role in the early events of gastric carcinogenesis and may be involved in invasion and metastasis of GC.
