Colonic mucus and gut microbiota closely linked through mutual regulatory effect.While their alterations during sepsis and whether glutamine can maintain the colonic mucus barrier and gut microbiota stability are uncl...Colonic mucus and gut microbiota closely linked through mutual regulatory effect.While their alterations during sepsis and whether glutamine can maintain the colonic mucus barrier and gut microbiota stability are unclear.Cecal ligation and puncture-induced sepsis in mice was utilized to observe changes in colonic mucus,gut microbiota,and their interaction with glutamine intervention.Our findings indicated that glutamine mitigated sepsis-induced intestinal damage and restores colonic mucus barrier function by augmenting mucin synthesis.Further analysis revealed that goblet cells were under oxidative stress after sepsis,resulting in anterior gradient-2(AGR2),the key mucin-modifying enzyme,being dissynthesized,and inhibiting mucin 2(MUC2)maturation.Glutamine could ameliorates this situation by promoting the key enzyme glucose-6-phosphate dehydrogenase(G6PD)glycosylation in the pentose phosphate pathway,increasing the reduced nicotinamide adenine dinucleotide phosphate(NADPH)synthesis,reducing endoplasmic reticulum stress,and accelerating AGR2 synthesis and MUC2 maturation.Additionally,glutamine aided in maintaining gut microbiota stability during sepsis,up-regulating mucin-associated bacteria such as Akkermansia and Alistipes.These bacteria,intimately linked to mucin synthesis and degradation,may impact intestinal mucus stability.In conclusion,glutamine can maintain goblet cell redox balance,promotes AGR2 synthesis and MUC2 maturation,shields the mucus barrier,and potentially maintains gut microbiota stability by regulating the interaction between bacteria and mucus,thus alleviating sepsis-induced intestinal damage.展开更多
2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题目为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like ce...2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题目为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like cells in small cell lung cancer”的研究论文。展开更多
2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like cell...2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like cells in small cell lung cancer”的研究论文。展开更多
基金supported by the National Natural Science Foundation of China(82172202)Innovative Leading Talents Project of Chongqing(cstc2022ycjh-bgzxm0148)+1 种基金the Project of NINGBO Leading Medical&Health Discipline(2022-F17)Ningbo Top Medical and Health Research Program(2023030615).
文摘Colonic mucus and gut microbiota closely linked through mutual regulatory effect.While their alterations during sepsis and whether glutamine can maintain the colonic mucus barrier and gut microbiota stability are unclear.Cecal ligation and puncture-induced sepsis in mice was utilized to observe changes in colonic mucus,gut microbiota,and their interaction with glutamine intervention.Our findings indicated that glutamine mitigated sepsis-induced intestinal damage and restores colonic mucus barrier function by augmenting mucin synthesis.Further analysis revealed that goblet cells were under oxidative stress after sepsis,resulting in anterior gradient-2(AGR2),the key mucin-modifying enzyme,being dissynthesized,and inhibiting mucin 2(MUC2)maturation.Glutamine could ameliorates this situation by promoting the key enzyme glucose-6-phosphate dehydrogenase(G6PD)glycosylation in the pentose phosphate pathway,increasing the reduced nicotinamide adenine dinucleotide phosphate(NADPH)synthesis,reducing endoplasmic reticulum stress,and accelerating AGR2 synthesis and MUC2 maturation.Additionally,glutamine aided in maintaining gut microbiota stability during sepsis,up-regulating mucin-associated bacteria such as Akkermansia and Alistipes.These bacteria,intimately linked to mucin synthesis and degradation,may impact intestinal mucus stability.In conclusion,glutamine can maintain goblet cell redox balance,promotes AGR2 synthesis and MUC2 maturation,shields the mucus barrier,and potentially maintains gut microbiota stability by regulating the interaction between bacteria and mucus,thus alleviating sepsis-induced intestinal damage.
文摘2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题目为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like cells in small cell lung cancer”的研究论文。
文摘2025年5月12日,上海交通大学医学院组织胚胎与遗传发育学系黄雷课题组联合中南大学附属湘雅医院胡成平团队在国际知名期刊Advanced Science上发表了题为“PP2A promotes the symmetric division of MUC1-dominant cancer stem-like cells in small cell lung cancer”的研究论文。
