AIM: To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers. METHODS: Methylation-specific P...AIM: To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers. METHODS: Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens. RESULTS: p16 methylation was detected in 42% of the tumors.Dukes'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%). CONCLUSION: p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.展开更多
Pure ZnO, ZnO–CuO nanocomposites can be synthesized by using a modified perfume spray pyrolysis method（MSP）. The crystallite size of the nanoparticles（NPs） has been observed by X-ray diffraction pattern and is ne...Pure ZnO, ZnO–CuO nanocomposites can be synthesized by using a modified perfume spray pyrolysis method（MSP）. The crystallite size of the nanoparticles（NPs） has been observed by X-ray diffraction pattern and is nearly 36 nm. Morphological studies have been analyzed by using Field Emission Scanning Electron Microscopy（FESEM） and its elemental analysis was reported by Elemental X-ray Analysis（EDX）; these studies confirmed that ZnO and CuO have hexagonal structure and monoclinic structure respectively. Fourier Transform Infrared（FTIR） spectra revealed that the presence of functional frequencies of ZnO and CuO were observed at 443 and 616 cm-1.The average bandgap value at 3.25 e V using UV–vis spectra for the entitled composite has described a blue shift that has been observed here. The antibacterial study against both gram positive and negative bacteria has been studied by the disc diffusion method. To the best of our knowledge, it is the first report on ZnO–CuO nanocomposite synthesized by a modified perfume spray pyrolysis method.展开更多
基金Supported by the grants from Mjnistry of Public Health of China,No.98-1-303The Educational Committee of Shanghai,No.2000B02.
文摘AIM: To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers. METHODS: Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens. RESULTS: p16 methylation was detected in 42% of the tumors.Dukes'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%). CONCLUSION: p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.
文摘Pure ZnO, ZnO–CuO nanocomposites can be synthesized by using a modified perfume spray pyrolysis method（MSP）. The crystallite size of the nanoparticles（NPs） has been observed by X-ray diffraction pattern and is nearly 36 nm. Morphological studies have been analyzed by using Field Emission Scanning Electron Microscopy（FESEM） and its elemental analysis was reported by Elemental X-ray Analysis（EDX）; these studies confirmed that ZnO and CuO have hexagonal structure and monoclinic structure respectively. Fourier Transform Infrared（FTIR） spectra revealed that the presence of functional frequencies of ZnO and CuO were observed at 443 and 616 cm-1.The average bandgap value at 3.25 e V using UV–vis spectra for the entitled composite has described a blue shift that has been observed here. The antibacterial study against both gram positive and negative bacteria has been studied by the disc diffusion method. To the best of our knowledge, it is the first report on ZnO–CuO nanocomposite synthesized by a modified perfume spray pyrolysis method.
