Rice, the world's primary staple food, is under severe threat from several devastating diseases. To sustainably management rice diseases, developing safe, environmentally friendly alternatives urgently need to be ...Rice, the world's primary staple food, is under severe threat from several devastating diseases. To sustainably management rice diseases, developing safe, environmentally friendly alternatives urgently need to be developed. In this study, we synthesized two silicon dioxide nanoparticles, spherical mesoporous silica nanoparticles (MSNs) and virus-like mesoporous silica nanoparticles (VMSNs), and we performed a resistance assay on rice for two major diseases, bacterial blight caused by Xanthomonas oryzae pv. oryzae and sheath blight caused by Rhizoctonia solani. Compared to the control, the two nanoparticle treatments increased rice resistance, with VMSNs exhibiting the highest efficacy in controlling these two diseases, causing a shorter lesion length than those plants treated by MSNs. Coincidentally, the foliar application of VMSNs activates a higher expression level of several pathogenesis-related (PR) genes compared to those of MSNs and Si_(2) treatment. By using fluorescein isothiocyanate (FITC)-labeled VMSNs (VMSNs-FITC) to soak the top expanded leaf tips or roots, we observed that the fluorescence of the nanoparticles firstly accumulated at the local site of the top leaves or roots, rapidly migrated to the hypocotyl of rice, and then redistributed sequentially from the bottom leaves to the upper leaves. Furthermore, the foliar or root application of VMSNs-FITC could trigger the local and systemic resistance to PXO99. Notably, no significant toxicity was observed on plants and mice after excessive foliar treatment or feeding tests, respectively. Overall, our research revealed that VMSNs are an effective, systemic, and safe nano-pesticides for controlling rice diseases. Boosting the immune responses may be associated with the transporting of nanoparticles.展开更多
ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-function...ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.展开更多
基金funded by grants from the National Natural Science Foundation of China(No.52373305)Natural Science Foundation of Hubei Province(2024AFB824,2023AFB661 and B2022015)+2 种基金the Key Laboratory of Integrated Management of Crops of Central China and Hubei Key Laboratory of Crop Disease,Insect Pests and Weeds Control(Grant No.2023ZTSJJ4)Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology(No.OHIC2022G11)the Natural Science Foundation of Hubei Province(2024AFB824).
文摘Rice, the world's primary staple food, is under severe threat from several devastating diseases. To sustainably management rice diseases, developing safe, environmentally friendly alternatives urgently need to be developed. In this study, we synthesized two silicon dioxide nanoparticles, spherical mesoporous silica nanoparticles (MSNs) and virus-like mesoporous silica nanoparticles (VMSNs), and we performed a resistance assay on rice for two major diseases, bacterial blight caused by Xanthomonas oryzae pv. oryzae and sheath blight caused by Rhizoctonia solani. Compared to the control, the two nanoparticle treatments increased rice resistance, with VMSNs exhibiting the highest efficacy in controlling these two diseases, causing a shorter lesion length than those plants treated by MSNs. Coincidentally, the foliar application of VMSNs activates a higher expression level of several pathogenesis-related (PR) genes compared to those of MSNs and Si_(2) treatment. By using fluorescein isothiocyanate (FITC)-labeled VMSNs (VMSNs-FITC) to soak the top expanded leaf tips or roots, we observed that the fluorescence of the nanoparticles firstly accumulated at the local site of the top leaves or roots, rapidly migrated to the hypocotyl of rice, and then redistributed sequentially from the bottom leaves to the upper leaves. Furthermore, the foliar or root application of VMSNs-FITC could trigger the local and systemic resistance to PXO99. Notably, no significant toxicity was observed on plants and mice after excessive foliar treatment or feeding tests, respectively. Overall, our research revealed that VMSNs are an effective, systemic, and safe nano-pesticides for controlling rice diseases. Boosting the immune responses may be associated with the transporting of nanoparticles.
基金National Key R&D Program of China(No.2017YFA0205100)the National Natural Science Foundation of China(No.22101052)for financial support.
文摘ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.
