目的探究巨噬细胞表面特异分子跨膜4域亚家族A成员6D(membrane-spanning 4-domains subfamily A member 6D,Ms4a6d)基因敲除对雌性小鼠生育力的影响。方法构建Ms4a6d基因敲除小鼠,以各周龄纯合Ms4a6d基因敲除(Ms4a6d^(-/-))小鼠为实验组...目的探究巨噬细胞表面特异分子跨膜4域亚家族A成员6D(membrane-spanning 4-domains subfamily A member 6D,Ms4a6d)基因敲除对雌性小鼠生育力的影响。方法构建Ms4a6d基因敲除小鼠,以各周龄纯合Ms4a6d基因敲除(Ms4a6d^(-/-))小鼠为实验组;采用qPCR、琼脂糖凝胶法鉴定小鼠基因型;运用HE染色、免疫荧光、ELISA等方法检测血清抗缪勒管激素(anti-müllerian hormone,AMH)和雌性Ms4a6d^(-/-)小鼠卵巢中巨噬细胞数量及各级卵泡构成变化;通过生育力实验比较成年Ms4a6d^(-/-)雌鼠妊娠率及平均产仔数变化。结果与同周龄野生型(Ms4a6d+/+)雌鼠比较,2周龄和4周龄的Ms4a6d^(-/-)雌鼠卵巢组织中巨噬细胞显著减少(P<0.01);8周龄时2组卵巢巨噬细胞差异无统计学意义;8周龄Ms4a6d^(-/-)雌鼠卵巢系数显著降低(P<0.01);8周龄时卵巢组织中原始卵泡、初级卵泡、次级卵泡和窦卵泡数量显著减少(P<0.05),各年龄段Ms4a6d^(-/-)雌鼠血清中AMH显著降低(P<0.05)。成年Ms4a6d^(-/-)雌鼠的平均妊娠率为56%,每胎产仔数为(4.1±1.1)只,均显著低于同龄野生型雌鼠的妊娠率(89%)和每胎产仔数[(6.3±1.2)只]。结论Ms4a6d基因敲除导致青春期前雌鼠卵巢组织中巨噬细胞数量减少,抑制其生长卵泡发育,最终表现为成年雌鼠卵巢储备减少,生育力降低。展开更多
Based on the network monitoring data and field investigations,the basic characteristics of the M S4.9 earthquake at the juncture of Gaoyou and Baoying in Jiangsu Province on July20,2012 have been introduced,and the fo...Based on the network monitoring data and field investigations,the basic characteristics of the M S4.9 earthquake at the juncture of Gaoyou and Baoying in Jiangsu Province on July20,2012 have been introduced,and the focal mechanism solutions have also been obtained.According to the regional tectonic features and site macroscopic survey,the occurrence of the earthquake was probably related to the Yangchacang-Sangshutou fault.Based on site survey data,both the evaluation criterion of seismic intensity of this region and the seismic intensity maps have been proposed.According to the damage characteristics of brick-concrete structure and brick-wood structure,the main reasons of serious damage for buildings are poor foundation conditions,low seismic bearing capacity,poor quality of construction and disrepair.Considering the differences of earthquake damage in the different regions,advice on accelerating the new rural construction,improving the resistance against earthquake disasters of rural residential areas and strengthening the seismogenic research on strong and moderate size earthquake is proposed,which will be possibly helpful for earthquake damage survey,prediction and prevention.展开更多
文摘目的探究巨噬细胞表面特异分子跨膜4域亚家族A成员6D(membrane-spanning 4-domains subfamily A member 6D,Ms4a6d)基因敲除对雌性小鼠生育力的影响。方法构建Ms4a6d基因敲除小鼠,以各周龄纯合Ms4a6d基因敲除(Ms4a6d^(-/-))小鼠为实验组;采用qPCR、琼脂糖凝胶法鉴定小鼠基因型;运用HE染色、免疫荧光、ELISA等方法检测血清抗缪勒管激素(anti-müllerian hormone,AMH)和雌性Ms4a6d^(-/-)小鼠卵巢中巨噬细胞数量及各级卵泡构成变化;通过生育力实验比较成年Ms4a6d^(-/-)雌鼠妊娠率及平均产仔数变化。结果与同周龄野生型(Ms4a6d+/+)雌鼠比较,2周龄和4周龄的Ms4a6d^(-/-)雌鼠卵巢组织中巨噬细胞显著减少(P<0.01);8周龄时2组卵巢巨噬细胞差异无统计学意义;8周龄Ms4a6d^(-/-)雌鼠卵巢系数显著降低(P<0.01);8周龄时卵巢组织中原始卵泡、初级卵泡、次级卵泡和窦卵泡数量显著减少(P<0.05),各年龄段Ms4a6d^(-/-)雌鼠血清中AMH显著降低(P<0.05)。成年Ms4a6d^(-/-)雌鼠的平均妊娠率为56%,每胎产仔数为(4.1±1.1)只,均显著低于同龄野生型雌鼠的妊娠率(89%)和每胎产仔数[(6.3±1.2)只]。结论Ms4a6d基因敲除导致青春期前雌鼠卵巢组织中巨噬细胞数量减少,抑制其生长卵泡发育,最终表现为成年雌鼠卵巢储备减少,生育力降低。
基金Supported by Key Project of Suzhou Municipal Government,China(No.2009-191)Key Project of Xuzhou Municipal Government,China(T(2009)009)
文摘Based on the network monitoring data and field investigations,the basic characteristics of the M S4.9 earthquake at the juncture of Gaoyou and Baoying in Jiangsu Province on July20,2012 have been introduced,and the focal mechanism solutions have also been obtained.According to the regional tectonic features and site macroscopic survey,the occurrence of the earthquake was probably related to the Yangchacang-Sangshutou fault.Based on site survey data,both the evaluation criterion of seismic intensity of this region and the seismic intensity maps have been proposed.According to the damage characteristics of brick-concrete structure and brick-wood structure,the main reasons of serious damage for buildings are poor foundation conditions,low seismic bearing capacity,poor quality of construction and disrepair.Considering the differences of earthquake damage in the different regions,advice on accelerating the new rural construction,improving the resistance against earthquake disasters of rural residential areas and strengthening the seismogenic research on strong and moderate size earthquake is proposed,which will be possibly helpful for earthquake damage survey,prediction and prevention.
