Objective: To investigate the composition of volatile compounds in the different accessions of Perilla frutescens(P. frutescens) collected from various habitats of China and Japan. Methods: In the present study, the e...Objective: To investigate the composition of volatile compounds in the different accessions of Perilla frutescens(P. frutescens) collected from various habitats of China and Japan. Methods: In the present study, the essential oil from the leaves of P. frutescens cultivars from China and Japan was extracted by hydro-distillation and the chemical composition and concentration of the volatile components present in the oils were determined by gas chromatography–mass spectrometry(GC–MS) analysis. Results: Among the volatile components, the major proportion was of perilla ketone, which was followed by elemicin and beta-caryophyllene in the Chinese Perilla cultivars. The main component in the oil extracted from the Japanese accessions was myristicin, which was followed by perilla ketone and beta-caryophyllene. We could distinguish seven chemotypes, namely the perilla ketone(PK) type, perilla ketone, myristicin(PM) type, perilla ketone, unknown(PU) type, perilla ketone, beta-caryophyllene, myristicine(PB) type, perilla ketone, myristicin, unknown(PMU) type, perilla ketone, elemicine, myristicin, beta-caryophyllene(PEMB) type, and the perilla ketone, limonene, betacryophyllene, myristicin(L) type. Most of the accessions possessed higher essential oil content before the flowering time than at the flowering stage. The average plant height, leaf length, leaf width of the Chinese accessions was higher than those of the Japanese accessions. Conclusion:The results revealed that the harvest time and geographical origin caused polymorphisms in the essential oil composition and morphological traits in the Perilla accessions originating from China and Japan. Therefore, these chemotypes with desirable characters might be useful for industrial exploitation and for determining the harvest time.展开更多
Shenfu Coal was extracted with hot pure water and slurry was isolated. The concentrated benzene-soluble fraction (CBSF) was analyzed with GC/MS and four types of organic compounds (OCs) were detected: HACOCs,DTEs,DMDT...Shenfu Coal was extracted with hot pure water and slurry was isolated. The concentrated benzene-soluble fraction (CBSF) was analyzed with GC/MS and four types of organic compounds (OCs) were detected: HACOCs,DTEs,DMDT and LCAs. The amount of benzyl benzoate which is the most abundant OC was calculated by an inter-nal standard method with an indicated amount of BP. The broken hydrogen bonds and ether bonds were responsible for the extraction of OCs from the coal .DTEs,DMDT and LCAs are essentially insoluble in water,whereas they are soluble,probably owing to intermolecular interaction of OCs with HACOCs.展开更多
Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods:...Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.展开更多
Ozonolysis products of four lepidopteral pheromone were identified by methane CI-MS.The spectra obtained were notably simpler than those of EI-MS,the peak of molecular ion was normally close to the base peak,and the c...Ozonolysis products of four lepidopteral pheromone were identified by methane CI-MS.The spectra obtained were notably simpler than those of EI-MS,the peak of molecular ion was normally close to the base peak,and the characteristic fragment ions were high in m/z,thereby the interpretation was facilitated.展开更多
Traditional medicinal plants are one of the potential sources of antimicrobial drugs and there is a great concern in the use and development of herbal medicine for the treatment of various infections. This study aimed...Traditional medicinal plants are one of the potential sources of antimicrobial drugs and there is a great concern in the use and development of herbal medicine for the treatment of various infections. This study aimed to evaluate the antibacterial, and antioxidant activities of Dichrostachys cinera ethanolic leaves extract and to determine the components of the crude extract. D. cinera extract was evaluated against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The antibacterial, antioxidant activities and active constituents were determined using standard methods. Antibacterial activity of the crude extract findings showed that all bacterial candidates were susceptible where S. aureus represent MIC at 12.5 mg/ml and MBC at 25 mg/ml, E. coli and P. aeruginosa both showed MIC 25 mg/ml and MBC 50 mg/ml. In the free radical scavenging assay of the extract and the standard quercetin at concentrations of 250 μg/ml, 125 μg/ml, 50 μg/ml, 10 μg/ml, and 5 μg/ml. The radical scavenging activity for the extract was about 92%, 89.6%, 86.8%, 82.8% and 37.8% respectively, compared to quercetin which gave 89.7%, 85.8%, 62.1%, 55.5%, and 45% radical scavenging activity. The GC-Ms analysis of the total constituents demonstrated that 1,6-Anhydro-2,4-dideoxy-.beta.-D-ribo-hexo (21.26%) with different peaks, followed by Glycerin (11.56%), 1,2,3-Cyclopentanetriol (10.18%), 8,11,14-Eicosatrienoic acid, (Z,Z,Z)-(6.18%), 1H-Pyrrole, 1-methyl-(6.08%), Phytol (5.91%) and 7-Bromo-6-(2-diethylaminoethoxy)-2,3-dihyd (5.44%) as major components in the extract. Finally, this study provided useful information on the therapeutic potential of D. cinera as an antibacterial agent and recommended to be evaluated against a wide range of Bacterial and fungal strains using different solvents and different parts from the plant.展开更多
Microcystins(MCs),a family of cyclic heptapeptide cyanotoxins,exists in aquatic environment where cyanobacterial bloom happens,which will accumulate in aquatic organisms and transfer through the food chain to higher t...Microcystins(MCs),a family of cyclic heptapeptide cyanotoxins,exists in aquatic environment where cyanobacterial bloom happens,which will accumulate in aquatic organisms and transfer through the food chain to higher trophic levels,posing a health risk to both animals and human bodies.Among various MCs,Microcystin-LR(MC-LR)is worthiest studied for its strong toxicity,ubiquity and widespread.Here in this work,iminodiacetic acid(IDA)decorated magnetic mesoporous silica(mSiO_(2))nanocomposites(Fe_(3)O_(4)@mSiO_(2)-IDA)were facilely synthesized which possessed the merits of large surface area(188.21 m^(2)/g),accessible porosity(2.66 nm),excellent hydrophilicity and rapid responsiveness to magnetic field.Then the composites were successfully employed to the removal process of Microcystin-LR in real water samples followed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)analysis,achieving the removal efficiency above 92.5%even after ten recycles of the composites.It provided a potential method for removing MC-LR in aqueous environment with high effectiveness,lower costs and less secondary contamination.展开更多
In this editorial,we comment on a recent publication,which highlights the important findings from the study,including the antitumor and anti-inflammatory effects of isothiocyanates,their underlying mechanisms,and impl...In this editorial,we comment on a recent publication,which highlights the important findings from the study,including the antitumor and anti-inflammatory effects of isothiocyanates,their underlying mechanisms,and implications.Additionally,a related perspective is discussed.展开更多
Background:Gut microbiota is an important part of blood transformation of oral Chinese herb,and the interaction with Taohong Siwu decoction(THSWD)will affect the body function.However,it is unclear how exactly THSWD i...Background:Gut microbiota is an important part of blood transformation of oral Chinese herb,and the interaction with Taohong Siwu decoction(THSWD)will affect the body function.However,it is unclear how exactly THSWD is regulated by gut microbiota.The aim of this study was to reveal the role of gut microbes in THSWD drug components through serum pharmacology and LC-MS/MS analysis.Methods:Specific pathogen free(SPF)and germ-free(GF)mice were divided into the SPF standard control group,THSWD intervention SPF group,and THSWD intervention GF group.The experimental group was treated with THSWD,and the control group was given the same amount of normal saline.After continuous intervention for a week,the serum was taken to detect the pharmaceutical ingredients of THSWD by LC-MS/MS analysis.Results:We revealed a total of 631 known-active components in THSWD.Hexylamine,Cuelure,and others found in THSWD were significantly increased in SPF mice after treated with THSWD.Compared with the intervention of THSWD in GF mice,Curcolone,Polyporusterone A,and others decreased significantly in SPF mice with THSWD,while Oxyresveratrol and FA 18:2+1O were increased in SPF mice after treated with THSWD.Conclusion:Gut microbiota may play the role of“transport messenger”,“processing plant”and“energy absorption”in the process of blood transformation of THSWD.It preliminarily reveals the gut microbial effect on THSWD,which is of great significance to reveal the transport of ingredients in Chinese herb,playing a guiding and promoting role in the modernization of Chinese herb.展开更多
Volatile oil was extracted from Illicium Verum Hook. f. by using steam distillation. 41 kinds of compounds were separated and identified by GC/MS, and their relative contents were determined by normalization method. A...Volatile oil was extracted from Illicium Verum Hook. f. by using steam distillation. 41 kinds of compounds were separated and identified by GC/MS, and their relative contents were determined by normalization method. Among 41 identified compounds, there are 14 hydrocarbon components and 22 oxygenated hydrocarbon derivatives, and a small amount of nitrogenous compounds. The main component is anethole, accoun ting for 76.23%, and the other components such as anisyl acetone, anisaldehyde, p allylanisole, p cumic aldehyde and p allylpen take up more than 10%.展开更多
In the present study, we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and...In the present study, we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and liver after oral administration. Rats were orally administrated with GBA at the dose of 50mg/kg body weight. GBA concentrations in blood plasma and liver were detected by HPLC from 0.5–10 h post administrations. The pharmacokinetic parameters were analyzed by the DAS1.0Software. The concentration-time curves of the plasma GBA and liver GBA wereconformed to two-compartment model. GBA was rapidly absorbed in the gastrointestinal tract and could be detected in plasma and liver 0.5 h after oral administration.展开更多
The thermal degradation of two synthetic lubricants base oils, poly-a-olefins （PAO） and di-esters （DE）, was investigated under oxidative pyrolysis condition and their properties were characterized in simulated ＂a...The thermal degradation of two synthetic lubricants base oils, poly-a-olefins （PAO） and di-esters （DE）, was investigated under oxidative pyrolysis condition and their properties were characterized in simulated ＂areo-engine＂ by comparing the thermal stability and identifying the products of thermal decomposition as a function of exposure temperature. The characterization of the products were performed by means of Fourier transform infrared spectrometry （FTIR）, gas chromatography/mass spectrometry （GC/MS） and viscosity experiments. The results show that PAO has the lower thermal stability, being degraded at 200℃ different from 300 ℃ for DE. Several by-products are identified during the thermal degradation of two lubricant base oils. The majority of PAO products consist of alkenes and olefins, while more oxygen-contained organic compounds are detected in DE samples based on GC/MS analysis. The related reaction mechanisms are discussed based on the experimental results.展开更多
The effect of different factors on coal extraction yield-temperature, solvent, coal type and irradiation time were investigated for different China coals under microwave-assisted extraction（MAE）. The results show th...The effect of different factors on coal extraction yield-temperature, solvent, coal type and irradiation time were investigated for different China coals under microwave-assisted extraction（MAE）. The results show that the optimum temperature for ethanol, acetone and THF are 393,373 and 373 K respectively and the extraction yield of THF is higher than that of other two solvents under MAE. Extraction with several single organic solvents were conducted for the 5 typical China coals-Shenfu, Tongchuan, Panzhihua, Yitai and Huating coal under microwave-assisted extraction and Soxhlet extraction. Results indicat that extraction yield of ethylenediamine, ethanol, acetone, tetrahydrofuran under MAE are all higher than that of Soxhlet extraction and MAE required less time. Tongchuan coal extracts of ethanol and acetone which were extracted by MAE and Soxhlet extraction were analyzed by GC/MS.展开更多
A solid phase extraction procedure(SPE)is described for the quantitative analysis of polycyclic aromatic hydrocarbons(PAHs)in atmospheric particulate matter(PM),as ubiquitous environmental pollutants routinely measure...A solid phase extraction procedure(SPE)is described for the quantitative analysis of polycyclic aromatic hydrocarbons(PAHs)in atmospheric particulate matter(PM),as ubiquitous environmental pollutants routinely measured in air qualitymonitoring.A SPE cartridge was used based on a molecular imprinted polymer(MIP-SPE)properly tailored for selective retention of PAHs with 4 and more benzene fused rings.The performance of the clean-up procedure was evaluated with the specific concern of selective purification towards saturated hydrocarbons,which are the PM components mostly interfering GC analysis of target PAHs.Under optimized operative conditions,the MIP-SPE provided analyte recovery close to 95%for heavier PAHs,from benzo(α)pyrene to benzo(ghi)perylene,and close to 90%for four benzene rings PAHs,with good reproducibility(RSDs:2.5%-5.9%).Otherwise,C_(17)-C_(32) n-alkanes were nearly completely removed.The proposed method was critically compared with Solid Phase Micro Extraction(SPME)using a polyacrylate fiber.Both methods were successfully applied to the analysis of ambient PM2.5 samples collected at an urban polluted site.Between the two procedures,the MIP-SPE provided the highest recovery(R%≥93%)for PAHs with 5 and more benzene rings,but lower for lighter PAHs.In contrast,SPME showed a mean acceptable R%value(∼80%)for all the investigated PAHs,except for the heaviest PAHs in the most polluted samples(R%:110%-138%),suggesting an incomplete purification from the interfering n-hydrocarbons.展开更多
Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillati...Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillation of Rabdosia lophanthoides yielded a pale yellow oil. The compounds identified and their relative proportions are listed in Table 1 according to their order of elution on an HP 5MS capillary column. .展开更多
A high-temperature coal tar collected from Xuzhou Coking Plant, Saint-Gobain Pipelines （Xuzhou） Co., Ltd. was exhaustively extracted with petroleum ether （PE）. The extract was sequentially eluted through silica ge...A high-temperature coal tar collected from Xuzhou Coking Plant, Saint-Gobain Pipelines （Xuzhou） Co., Ltd. was exhaustively extracted with petroleum ether （PE）. The extract was sequentially eluted through silica gel with PE and PE/ethyl acetate （EA） mixed solvents （volume ratio 2：1 ） to afford two eluted frac- tions. The extract and eluted fractions were analyzed with gas chromatography/mass spectrometry. In total, 14 arenols were detected, including phenol, cresols, xylenols, fluorenols, phenanthren-3-ol and pyren-l-ol.展开更多
The Mengya’a Lead–zinc deposit is a large skarn deposit in the north of the eastern segment of Gangdese metallogenic belt.The garnet is the main altered mineral in the Mengya’a area.The color of the garnet varies f...The Mengya’a Lead–zinc deposit is a large skarn deposit in the north of the eastern segment of Gangdese metallogenic belt.The garnet is the main altered mineral in the Mengya’a area.The color of the garnet varies from chartreuse to dark yellow brown and to russet.The brown garnet(Grt1)is related to pyrrhotite and chalcopyrite,and the green garnet(Grt2)is associated with lead–zinc mineralization.LA-ICP-MS is the induced coupled plasma mass spectrometry.This paper has used this technique to investigate Grt1 and Grt2.Grt1 develops core–rim textures with strong oscillation zone occurring in rim,whereas Grt2lacks core–rim textures and featured by oscillation zone.LA–ICP–MS analysis shows that garnets of Mengya’a are rich in CaO(29.90–37.52%)and FeO(21.17–33.35%),but low in Al_(2)O_(3)(0.05–4.85%).The calculated end members belong to grandite(grossular–andradite)garnets andradite.The negative Al(IV)versus Fe^(3+),positive Al(IV)versus total Al stoichiometric number,the positive Al(IV)versus Fe^(3+),and the negative Al(IV)versus total REE,all indicate that the substitution of REEs in garnets is controlled by YAG.All Garnets are depleted in large lithophile elements(e.g.,Rb=0.00–4.01 ppm,Sr=0.03–8.56 ppm).The total REE in Grt1 core is high(ΣREE=233–625 ppm),with HREE enriched pattern(LREE/HREE=0.33–1.69)and weak negative Eu anomalies(δEu=0.21–0.47).In contrast,the total REEs in the Grt1 rim and Grt2 are low(ΣREE=12.4–354 ppm;ΣREE=21.0–65.3 ppm),with LREE enriched pattern(LREE/HREE=0.54–34.4;LREE/HREE=11.4–682)and positive Eu anomalies(δEu=0.35–27.2;δEu=1.02–30.7).After data compilation of garnet chemicals,we found that the early fluid responsible for the core of Grt1 was a relatively closed and chloride-depleted fluid system.It was close-to-neutral,with a low water–rock ratio.The core of garnet was formed by fluid diffusion in metasomatic processes.The fluid was changed into a relatively open system with reduced,chloride-rich,and weakacid fluid.It was fluid infiltration and metasomatism that resulted in the formation of Grt1 rim and Grt2.展开更多
Performances of submerged membrane bioreactor （SMBR） treating brewery wastewater were investigated in this study. With little variation of COD：TN：TP ratio （100：5：1） in influent, SMBR showed high removal effici...Performances of submerged membrane bioreactor （SMBR） treating brewery wastewater were investigated in this study. With little variation of COD：TN：TP ratio （100：5：1） in influent, SMBR showed high removal efficiency （ 〉 90% ） for both COD and NH4^＋ - N, and it also showed a strong resistive ability for shock organics loading rate, evidenced by no obvious fluctuation for COD in the effluent when the organics loading rate suddenly increased from 0. 27 g/（ gMLSS · d） to 0. 54 g/（ gMLSS · d）. Comparatively different with the COD removal, TN and TP removal showed a strong correlation with the growth stage of the sludge in SMBR. When the sludge was in the multiplication stage, about 45% of TN was removed and an average removal efficiency of 30% for TP was also observed. However, when the activated sludge was in the steady stage, the removal efficiency for TN decreased to about 30% , whereas, the removal efficiency for TP was very low, and sometimes even below zero. The results of GC/MS indicated that the residual organic matters in the effluent were mainly alkyl hydrocarbon with high molecular weight, and coupling with the results of electroseopic scanning, it is speculated that biomass formed at external and internal membrane fibers played an important role for the removal of small organics.展开更多
This paper studied on the removal of toxic substance from river water using O3-GAC process. The result of GC/MS analysis indicated that the number of organic compound species was decreased by 55.1%. The species of tox...This paper studied on the removal of toxic substance from river water using O3-GAC process. The result of GC/MS analysis indicated that the number of organic compound species was decreased by 55.1%. The species of toxic substance of raw water also decreased from 16 to 5. The total removal rate of CODMn andUV254 were 45%～72% and 60%～80% following O3-GAC treatment. It reflected that this process had a good effective on removing unsaturation organic which absorb UV and toxic organic containing nitrogen. The results of Ames test indicated that raw water had a relatively strong mutagicity on TA 98. The O3-GAC process had a good ability in removing mutagen in water. The effluent water’s mutagicity is minus. The results of the study indicated that the effluent of the O3-GAC process was meet the demand of drinking water.展开更多
基金supported by funding from the Konkuk University Brain Pool
文摘Objective: To investigate the composition of volatile compounds in the different accessions of Perilla frutescens(P. frutescens) collected from various habitats of China and Japan. Methods: In the present study, the essential oil from the leaves of P. frutescens cultivars from China and Japan was extracted by hydro-distillation and the chemical composition and concentration of the volatile components present in the oils were determined by gas chromatography–mass spectrometry(GC–MS) analysis. Results: Among the volatile components, the major proportion was of perilla ketone, which was followed by elemicin and beta-caryophyllene in the Chinese Perilla cultivars. The main component in the oil extracted from the Japanese accessions was myristicin, which was followed by perilla ketone and beta-caryophyllene. We could distinguish seven chemotypes, namely the perilla ketone(PK) type, perilla ketone, myristicin(PM) type, perilla ketone, unknown(PU) type, perilla ketone, beta-caryophyllene, myristicine(PB) type, perilla ketone, myristicin, unknown(PMU) type, perilla ketone, elemicine, myristicin, beta-caryophyllene(PEMB) type, and the perilla ketone, limonene, betacryophyllene, myristicin(L) type. Most of the accessions possessed higher essential oil content before the flowering time than at the flowering stage. The average plant height, leaf length, leaf width of the Chinese accessions was higher than those of the Japanese accessions. Conclusion:The results revealed that the harvest time and geographical origin caused polymorphisms in the essential oil composition and morphological traits in the Perilla accessions originating from China and Japan. Therefore, these chemotypes with desirable characters might be useful for industrial exploitation and for determining the harvest time.
基金Project 90410018 supported by the National Natural Science Foundation of China.
文摘Shenfu Coal was extracted with hot pure water and slurry was isolated. The concentrated benzene-soluble fraction (CBSF) was analyzed with GC/MS and four types of organic compounds (OCs) were detected: HACOCs,DTEs,DMDT and LCAs. The amount of benzyl benzoate which is the most abundant OC was calculated by an inter-nal standard method with an indicated amount of BP. The broken hydrogen bonds and ether bonds were responsible for the extraction of OCs from the coal .DTEs,DMDT and LCAs are essentially insoluble in water,whereas they are soluble,probably owing to intermolecular interaction of OCs with HACOCs.
基金supported by Faculty of Pharmacy and Office of Grants, Endowments and Partnerships in Higher EducationUniversity of Santo Tomas, Manila, Philippines+2 种基金Nanocast Lab, Department of Chemistry, Mahidol University, Bangkok, ThailandDepartment of Science and Technology, PhilippinesCommission on Higher Education, Philippines
文摘Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.
文摘Ozonolysis products of four lepidopteral pheromone were identified by methane CI-MS.The spectra obtained were notably simpler than those of EI-MS,the peak of molecular ion was normally close to the base peak,and the characteristic fragment ions were high in m/z,thereby the interpretation was facilitated.
文摘Traditional medicinal plants are one of the potential sources of antimicrobial drugs and there is a great concern in the use and development of herbal medicine for the treatment of various infections. This study aimed to evaluate the antibacterial, and antioxidant activities of Dichrostachys cinera ethanolic leaves extract and to determine the components of the crude extract. D. cinera extract was evaluated against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The antibacterial, antioxidant activities and active constituents were determined using standard methods. Antibacterial activity of the crude extract findings showed that all bacterial candidates were susceptible where S. aureus represent MIC at 12.5 mg/ml and MBC at 25 mg/ml, E. coli and P. aeruginosa both showed MIC 25 mg/ml and MBC 50 mg/ml. In the free radical scavenging assay of the extract and the standard quercetin at concentrations of 250 μg/ml, 125 μg/ml, 50 μg/ml, 10 μg/ml, and 5 μg/ml. The radical scavenging activity for the extract was about 92%, 89.6%, 86.8%, 82.8% and 37.8% respectively, compared to quercetin which gave 89.7%, 85.8%, 62.1%, 55.5%, and 45% radical scavenging activity. The GC-Ms analysis of the total constituents demonstrated that 1,6-Anhydro-2,4-dideoxy-.beta.-D-ribo-hexo (21.26%) with different peaks, followed by Glycerin (11.56%), 1,2,3-Cyclopentanetriol (10.18%), 8,11,14-Eicosatrienoic acid, (Z,Z,Z)-(6.18%), 1H-Pyrrole, 1-methyl-(6.08%), Phytol (5.91%) and 7-Bromo-6-(2-diethylaminoethoxy)-2,3-dihyd (5.44%) as major components in the extract. Finally, this study provided useful information on the therapeutic potential of D. cinera as an antibacterial agent and recommended to be evaluated against a wide range of Bacterial and fungal strains using different solvents and different parts from the plant.
基金financially supported by National Key R&D Program of China(No.2018YFA0507501)the National Natural Science Foundation of China(Nos.22074019,21425518,22004017,22205085,and 32160305)+2 种基金Key Science and Technology Project of Jiangxi Province(Nos.20213AAG01012 and 2022KSG01004)Water Science and Technology Project of Jiangxi Province(Nos.202124ZDKT19 and 202223YBKT07)Shanghai Sailing Program(No.20YF1405300).
文摘Microcystins(MCs),a family of cyclic heptapeptide cyanotoxins,exists in aquatic environment where cyanobacterial bloom happens,which will accumulate in aquatic organisms and transfer through the food chain to higher trophic levels,posing a health risk to both animals and human bodies.Among various MCs,Microcystin-LR(MC-LR)is worthiest studied for its strong toxicity,ubiquity and widespread.Here in this work,iminodiacetic acid(IDA)decorated magnetic mesoporous silica(mSiO_(2))nanocomposites(Fe_(3)O_(4)@mSiO_(2)-IDA)were facilely synthesized which possessed the merits of large surface area(188.21 m^(2)/g),accessible porosity(2.66 nm),excellent hydrophilicity and rapid responsiveness to magnetic field.Then the composites were successfully employed to the removal process of Microcystin-LR in real water samples followed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)analysis,achieving the removal efficiency above 92.5%even after ten recycles of the composites.It provided a potential method for removing MC-LR in aqueous environment with high effectiveness,lower costs and less secondary contamination.
文摘In this editorial,we comment on a recent publication,which highlights the important findings from the study,including the antitumor and anti-inflammatory effects of isothiocyanates,their underlying mechanisms,and implications.Additionally,a related perspective is discussed.
基金supported by the National Natural Sciences Foundation of China(82305072,82305064)Wuxi Municipal Health Commission Scientific Research Fund Youth Project(Q202106)+1 种基金The Taihu Lake Talent Project of Wuxi Science and Technology Bureau(K20221027)Doctoral talent startup fund of Affiliated Hospital of Jiangnan University.
文摘Background:Gut microbiota is an important part of blood transformation of oral Chinese herb,and the interaction with Taohong Siwu decoction(THSWD)will affect the body function.However,it is unclear how exactly THSWD is regulated by gut microbiota.The aim of this study was to reveal the role of gut microbes in THSWD drug components through serum pharmacology and LC-MS/MS analysis.Methods:Specific pathogen free(SPF)and germ-free(GF)mice were divided into the SPF standard control group,THSWD intervention SPF group,and THSWD intervention GF group.The experimental group was treated with THSWD,and the control group was given the same amount of normal saline.After continuous intervention for a week,the serum was taken to detect the pharmaceutical ingredients of THSWD by LC-MS/MS analysis.Results:We revealed a total of 631 known-active components in THSWD.Hexylamine,Cuelure,and others found in THSWD were significantly increased in SPF mice after treated with THSWD.Compared with the intervention of THSWD in GF mice,Curcolone,Polyporusterone A,and others decreased significantly in SPF mice with THSWD,while Oxyresveratrol and FA 18:2+1O were increased in SPF mice after treated with THSWD.Conclusion:Gut microbiota may play the role of“transport messenger”,“processing plant”and“energy absorption”in the process of blood transformation of THSWD.It preliminarily reveals the gut microbial effect on THSWD,which is of great significance to reveal the transport of ingredients in Chinese herb,playing a guiding and promoting role in the modernization of Chinese herb.
基金ScienceFoundationofEducationforHunanProvince (No .0 0C32 4 )
文摘Volatile oil was extracted from Illicium Verum Hook. f. by using steam distillation. 41 kinds of compounds were separated and identified by GC/MS, and their relative contents were determined by normalization method. Among 41 identified compounds, there are 14 hydrocarbon components and 22 oxygenated hydrocarbon derivatives, and a small amount of nitrogenous compounds. The main component is anethole, accoun ting for 76.23%, and the other components such as anisyl acetone, anisaldehyde, p allylanisole, p cumic aldehyde and p allylpen take up more than 10%.
基金Clinical Innovative Team of Chinese and Mongolian Medicine(Grant No.YKD2007KJCXTD008)
文摘In the present study, we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and liver after oral administration. Rats were orally administrated with GBA at the dose of 50mg/kg body weight. GBA concentrations in blood plasma and liver were detected by HPLC from 0.5–10 h post administrations. The pharmacokinetic parameters were analyzed by the DAS1.0Software. The concentration-time curves of the plasma GBA and liver GBA wereconformed to two-compartment model. GBA was rapidly absorbed in the gastrointestinal tract and could be detected in plasma and liver 0.5 h after oral administration.
基金Supported by the Fund from the Air Force Armament Department of China for Innovative Research Group(Grant KJ2012283)
文摘The thermal degradation of two synthetic lubricants base oils, poly-a-olefins （PAO） and di-esters （DE）, was investigated under oxidative pyrolysis condition and their properties were characterized in simulated ＂areo-engine＂ by comparing the thermal stability and identifying the products of thermal decomposition as a function of exposure temperature. The characterization of the products were performed by means of Fourier transform infrared spectrometry （FTIR）, gas chromatography/mass spectrometry （GC/MS） and viscosity experiments. The results show that PAO has the lower thermal stability, being degraded at 200℃ different from 300 ℃ for DE. Several by-products are identified during the thermal degradation of two lubricant base oils. The majority of PAO products consist of alkenes and olefins, while more oxygen-contained organic compounds are detected in DE samples based on GC/MS analysis. The related reaction mechanisms are discussed based on the experimental results.
文摘The effect of different factors on coal extraction yield-temperature, solvent, coal type and irradiation time were investigated for different China coals under microwave-assisted extraction（MAE）. The results show that the optimum temperature for ethanol, acetone and THF are 393,373 and 373 K respectively and the extraction yield of THF is higher than that of other two solvents under MAE. Extraction with several single organic solvents were conducted for the 5 typical China coals-Shenfu, Tongchuan, Panzhihua, Yitai and Huating coal under microwave-assisted extraction and Soxhlet extraction. Results indicat that extraction yield of ethylenediamine, ethanol, acetone, tetrahydrofuran under MAE are all higher than that of Soxhlet extraction and MAE required less time. Tongchuan coal extracts of ethanol and acetone which were extracted by MAE and Soxhlet extraction were analyzed by GC/MS.
基金supported by the Emilia Romagna Region (POR FESR 2014-2020),project IPA/BC-MONITOR (No. E32I16000030007 2016)
文摘A solid phase extraction procedure(SPE)is described for the quantitative analysis of polycyclic aromatic hydrocarbons(PAHs)in atmospheric particulate matter(PM),as ubiquitous environmental pollutants routinely measured in air qualitymonitoring.A SPE cartridge was used based on a molecular imprinted polymer(MIP-SPE)properly tailored for selective retention of PAHs with 4 and more benzene fused rings.The performance of the clean-up procedure was evaluated with the specific concern of selective purification towards saturated hydrocarbons,which are the PM components mostly interfering GC analysis of target PAHs.Under optimized operative conditions,the MIP-SPE provided analyte recovery close to 95%for heavier PAHs,from benzo(α)pyrene to benzo(ghi)perylene,and close to 90%for four benzene rings PAHs,with good reproducibility(RSDs:2.5%-5.9%).Otherwise,C_(17)-C_(32) n-alkanes were nearly completely removed.The proposed method was critically compared with Solid Phase Micro Extraction(SPME)using a polyacrylate fiber.Both methods were successfully applied to the analysis of ambient PM2.5 samples collected at an urban polluted site.Between the two procedures,the MIP-SPE provided the highest recovery(R%≥93%)for PAHs with 5 and more benzene rings,but lower for lighter PAHs.In contrast,SPME showed a mean acceptable R%value(∼80%)for all the investigated PAHs,except for the heaviest PAHs in the most polluted samples(R%:110%-138%),suggesting an incomplete purification from the interfering n-hydrocarbons.
文摘Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillation of Rabdosia lophanthoides yielded a pale yellow oil. The compounds identified and their relative proportions are listed in Table 1 according to their order of elution on an HP 5MS capillary column. .
基金subsidized by the Fund from the Natural Science Foundation of China for Innovative Research Group (Grant50921002)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘A high-temperature coal tar collected from Xuzhou Coking Plant, Saint-Gobain Pipelines （Xuzhou） Co., Ltd. was exhaustively extracted with petroleum ether （PE）. The extract was sequentially eluted through silica gel with PE and PE/ethyl acetate （EA） mixed solvents （volume ratio 2：1 ） to afford two eluted frac- tions. The extract and eluted fractions were analyzed with gas chromatography/mass spectrometry. In total, 14 arenols were detected, including phenol, cresols, xylenols, fluorenols, phenanthren-3-ol and pyren-l-ol.
基金funded by the Surface Project of National Natural Science Foundation of China(41372093)。
文摘The Mengya’a Lead–zinc deposit is a large skarn deposit in the north of the eastern segment of Gangdese metallogenic belt.The garnet is the main altered mineral in the Mengya’a area.The color of the garnet varies from chartreuse to dark yellow brown and to russet.The brown garnet(Grt1)is related to pyrrhotite and chalcopyrite,and the green garnet(Grt2)is associated with lead–zinc mineralization.LA-ICP-MS is the induced coupled plasma mass spectrometry.This paper has used this technique to investigate Grt1 and Grt2.Grt1 develops core–rim textures with strong oscillation zone occurring in rim,whereas Grt2lacks core–rim textures and featured by oscillation zone.LA–ICP–MS analysis shows that garnets of Mengya’a are rich in CaO(29.90–37.52%)and FeO(21.17–33.35%),but low in Al_(2)O_(3)(0.05–4.85%).The calculated end members belong to grandite(grossular–andradite)garnets andradite.The negative Al(IV)versus Fe^(3+),positive Al(IV)versus total Al stoichiometric number,the positive Al(IV)versus Fe^(3+),and the negative Al(IV)versus total REE,all indicate that the substitution of REEs in garnets is controlled by YAG.All Garnets are depleted in large lithophile elements(e.g.,Rb=0.00–4.01 ppm,Sr=0.03–8.56 ppm).The total REE in Grt1 core is high(ΣREE=233–625 ppm),with HREE enriched pattern(LREE/HREE=0.33–1.69)and weak negative Eu anomalies(δEu=0.21–0.47).In contrast,the total REEs in the Grt1 rim and Grt2 are low(ΣREE=12.4–354 ppm;ΣREE=21.0–65.3 ppm),with LREE enriched pattern(LREE/HREE=0.54–34.4;LREE/HREE=11.4–682)and positive Eu anomalies(δEu=0.35–27.2;δEu=1.02–30.7).After data compilation of garnet chemicals,we found that the early fluid responsible for the core of Grt1 was a relatively closed and chloride-depleted fluid system.It was close-to-neutral,with a low water–rock ratio.The core of garnet was formed by fluid diffusion in metasomatic processes.The fluid was changed into a relatively open system with reduced,chloride-rich,and weakacid fluid.It was fluid infiltration and metasomatism that resulted in the formation of Grt1 rim and Grt2.
文摘Performances of submerged membrane bioreactor （SMBR） treating brewery wastewater were investigated in this study. With little variation of COD：TN：TP ratio （100：5：1） in influent, SMBR showed high removal efficiency （ 〉 90% ） for both COD and NH4^＋ - N, and it also showed a strong resistive ability for shock organics loading rate, evidenced by no obvious fluctuation for COD in the effluent when the organics loading rate suddenly increased from 0. 27 g/（ gMLSS · d） to 0. 54 g/（ gMLSS · d）. Comparatively different with the COD removal, TN and TP removal showed a strong correlation with the growth stage of the sludge in SMBR. When the sludge was in the multiplication stage, about 45% of TN was removed and an average removal efficiency of 30% for TP was also observed. However, when the activated sludge was in the steady stage, the removal efficiency for TN decreased to about 30% , whereas, the removal efficiency for TP was very low, and sometimes even below zero. The results of GC/MS indicated that the residual organic matters in the effluent were mainly alkyl hydrocarbon with high molecular weight, and coupling with the results of electroseopic scanning, it is speculated that biomass formed at external and internal membrane fibers played an important role for the removal of small organics.
文摘This paper studied on the removal of toxic substance from river water using O3-GAC process. The result of GC/MS analysis indicated that the number of organic compound species was decreased by 55.1%. The species of toxic substance of raw water also decreased from 16 to 5. The total removal rate of CODMn andUV254 were 45%～72% and 60%～80% following O3-GAC treatment. It reflected that this process had a good effective on removing unsaturation organic which absorb UV and toxic organic containing nitrogen. The results of Ames test indicated that raw water had a relatively strong mutagicity on TA 98. The O3-GAC process had a good ability in removing mutagen in water. The effluent water’s mutagicity is minus. The results of the study indicated that the effluent of the O3-GAC process was meet the demand of drinking water.
