目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2...目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。展开更多
Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agent...Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agents.Approximately 119 compounds from 90 plants were recognized as potent anti-bacterials[2].Dalbergia odorifera,a traditional Chinese plant,has demonstrated anti-tumor,anti-microbial,anti-inflammatory,and cardiovascular protective effects[3].Limited studies have explored D.odorifera flavonoids'inhibitory activity against MRSA.Transcriptomics,a high-throughput method,aided in comprehending plant antibacterial therapy by generating data for gene expression,target identification,and pathway analysis[4].Consequently,our study aimed to assess D.odorifera's anti-MRSA effects and reveal its material foundation and antibacterial mechanism by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS),and transcriptomic analysis,in vitro,and in-silico studies.展开更多
文摘目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。
基金the National Natural Science Foundation of China(Grant No.:81973191)Shanghai Agriculture Applied Technology Development Program,China(Program No.:T20200104)the National Key Research and Development Program of China(Program No.:2018YFE0192600)for providing financial support for the complete undertaking.
文摘Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agents.Approximately 119 compounds from 90 plants were recognized as potent anti-bacterials[2].Dalbergia odorifera,a traditional Chinese plant,has demonstrated anti-tumor,anti-microbial,anti-inflammatory,and cardiovascular protective effects[3].Limited studies have explored D.odorifera flavonoids'inhibitory activity against MRSA.Transcriptomics,a high-throughput method,aided in comprehending plant antibacterial therapy by generating data for gene expression,target identification,and pathway analysis[4].Consequently,our study aimed to assess D.odorifera's anti-MRSA effects and reveal its material foundation and antibacterial mechanism by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS),and transcriptomic analysis,in vitro,and in-silico studies.
