Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood....Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.展开更多
Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechani...Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.展开更多
Inclusion bodies(IBs)of respiratory syncytial virus(RSV)are formed by liquid-liquid phase separation(LLPS)and contain internal structures termed“IB-associated granules”(IBAGs),where anti-termination factor M2-1 and ...Inclusion bodies(IBs)of respiratory syncytial virus(RSV)are formed by liquid-liquid phase separation(LLPS)and contain internal structures termed“IB-associated granules”(IBAGs),where anti-termination factor M2-1 and viral mRNAs are concentrated.However,the mechanism of IBAG formation and the physiological function of IBAGs are unclear.Here,we found that the internal structures of RSV IBs are actual M2-1-free viral messenger ribonucleoprotein(mRNP)condensates formed by secondary LLPS.Mechanistically,the RSV nucleoprotein(N)and M2-1 interact with and recruit PABP to IBs,promoting PABP to bind viral mRNAs transcribed in IBs by RNArecognition motif and drive secondary phase separation.Furthermore,PABP-eIF4G1 interaction regulates viral mRNP condensate composition,thereby recruiting specific translation initiation factors(eIF4G1,eIF4E,eIF4A,eIF4B and eIF4H)into the secondary condensed phase to activate viral mRNAs for ribosomal recruitment.Our study proposes a novel LLPS-regulated translation mechanism during viral infection and a novel antiviral strategy via targeting on secondary condensed phase.展开更多
Long noncoding RNAs(lncRNAs)are important in abiotic stress tolerance.Here,we identified salt-responsive genes and lncRNAs in the roots and leaves of Betula platyphylla Suk.(birch),and characterized their lncRNAs func...Long noncoding RNAs(lncRNAs)are important in abiotic stress tolerance.Here,we identified salt-responsive genes and lncRNAs in the roots and leaves of Betula platyphylla Suk.(birch),and characterized their lncRNAs functions.In total,2660 mRNAs and 539 lncRNAs responding to salt treatment were identified using RNA-seq.The salt-responsive genes were substantially enriched in‘cell wall biogenesis’and‘wood development’in the roots and were enriched in‘photosynthesis’and‘response to stimulus’in the leaves.Meanwhile,the potential target genes of the salt-responsive lncRNAs in roots and leaves were both enriched in‘nitrogen compound metabolic process’and‘response to stimulus’.We further built a method for quickly identifying abiotic stress tolerance of lncRNAs,which employed transient transformation for overexpression and knock-down of the lncRNA,enabling gain-and loss-of-function analysis.Using this method,11 randomly selected salt-responsive lncRNAs were characterized.Among them,six lncRNAs confer salt tolerance,two lncRNAs confer salt sensitivity,and the other three lncRNAs are not involved in salt tolerance.In addition,a lncRNA,LncY1,was further characterized,which improves salt tolerance by regulating two transcription factors,BpMYB96 and BpCDF3.Taken together,our results suggested that lncRNAs play important roles in the salt response of birch plants.展开更多
文摘Objectives:Recently,we and others have demonstrated the involvement of Zinc Finger Antisense 1(ZFAS1)in cancer development.However,the intricate interplay of ZFAS1 with miRNAs and mRNAs remains to be fully understood.Materials and methods:We followed PRISMA guidelines to retrieve and assess the available literature on the topic“ZFAS1/miRNA/mRNA axis”and“Cancer”from databases such as PubMed,Google Scholar,and ScienceDirect.We also used bioinformatic webtools for analyzing the potential miRNA targets of ZFAS1 and its role in survival of cancer patients along with their role in various biological functions and pathways.Results:Our literature search and bioinformatic analysis reveals that ZFAS1 serves as a sponge for numerous miRNAs.Among the various targeted miRNAs,miR-150-5p stands out as significantly correlated with ZFAS1 across multiple databases(p-value=3.27e-16,R-value=-0.346).Additionally,our Kaplan-Meier survival analysis indicates a noteworthy association between ZFAS1 expression levels and overall poor prognosis and survival rates in ovarian,sarcoma,and pancreatic cancers.We also underscore the involvement of various signaling pathways,including Signal Transducer and Activator of Transcription 3(STAT3),Spindle and Kinetochore-associated Protein 1(SKA1),Lysophosphatidic acid receptor 1(LPAR1),and Wntβ-catenin,in cancer development through the ZFAS1/miRNAs/mRNAs axis.Furthermore,we identify ZFAS1’s pivotal roles in diverse molecular processes,such as RNA binding and ribonucleoprotein formation.Conclusion:In conclusion,this review comprehensively summarizes the latest advancements in understanding the regulatory relationships among ZFAS1,miRNAs,and mRNAs,emphasizing their collective role in cancer development to propose innovative avenues for cancer treatment.We believe that the intricate relationship among the ZFAS1-miRNA-mRNA axis may yield potential therapeutic targets for effective cancer management.
基金supported by the 111 Project(Grant No.B17043)the 2115 Talent Development Program of China Agricultural University。
文摘Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.
基金supported by the grants from National Key R&D Program of China(2021YFC2300702 and 2021YFC2300200)the Hubei Provincial Natural Science Foundation of China(2021CFB364)+1 种基金the National Natural Science Foundation of China(82130064,81825015,U22A20337 and 32000119)the Key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory(JXBS001).
文摘Inclusion bodies(IBs)of respiratory syncytial virus(RSV)are formed by liquid-liquid phase separation(LLPS)and contain internal structures termed“IB-associated granules”(IBAGs),where anti-termination factor M2-1 and viral mRNAs are concentrated.However,the mechanism of IBAG formation and the physiological function of IBAGs are unclear.Here,we found that the internal structures of RSV IBs are actual M2-1-free viral messenger ribonucleoprotein(mRNP)condensates formed by secondary LLPS.Mechanistically,the RSV nucleoprotein(N)and M2-1 interact with and recruit PABP to IBs,promoting PABP to bind viral mRNAs transcribed in IBs by RNArecognition motif and drive secondary phase separation.Furthermore,PABP-eIF4G1 interaction regulates viral mRNP condensate composition,thereby recruiting specific translation initiation factors(eIF4G1,eIF4E,eIF4A,eIF4B and eIF4H)into the secondary condensed phase to activate viral mRNAs for ribosomal recruitment.Our study proposes a novel LLPS-regulated translation mechanism during viral infection and a novel antiviral strategy via targeting on secondary condensed phase.
基金supported by the Xingliao Talent Plan Project XLYC1902007Funds for Guiding Local Scientific and Technological Development by the Central Government 202JH6/10500071.
文摘Long noncoding RNAs(lncRNAs)are important in abiotic stress tolerance.Here,we identified salt-responsive genes and lncRNAs in the roots and leaves of Betula platyphylla Suk.(birch),and characterized their lncRNAs functions.In total,2660 mRNAs and 539 lncRNAs responding to salt treatment were identified using RNA-seq.The salt-responsive genes were substantially enriched in‘cell wall biogenesis’and‘wood development’in the roots and were enriched in‘photosynthesis’and‘response to stimulus’in the leaves.Meanwhile,the potential target genes of the salt-responsive lncRNAs in roots and leaves were both enriched in‘nitrogen compound metabolic process’and‘response to stimulus’.We further built a method for quickly identifying abiotic stress tolerance of lncRNAs,which employed transient transformation for overexpression and knock-down of the lncRNA,enabling gain-and loss-of-function analysis.Using this method,11 randomly selected salt-responsive lncRNAs were characterized.Among them,six lncRNAs confer salt tolerance,two lncRNAs confer salt sensitivity,and the other three lncRNAs are not involved in salt tolerance.In addition,a lncRNA,LncY1,was further characterized,which improves salt tolerance by regulating two transcription factors,BpMYB96 and BpCDF3.Taken together,our results suggested that lncRNAs play important roles in the salt response of birch plants.
