目的:通过骨癌痛(bone cancer pain,BCP)小鼠模型明确右美托咪定是否参与吗啡耐受并探索其机制。方法:使用Lewis肺癌细胞系制备C57BL/6小鼠股骨癌痛动物模型。对BCP小鼠进行鞘内置管,连续7天注射吗啡诱导耐受产生。von Frey纤维丝评估...目的:通过骨癌痛(bone cancer pain,BCP)小鼠模型明确右美托咪定是否参与吗啡耐受并探索其机制。方法:使用Lewis肺癌细胞系制备C57BL/6小鼠股骨癌痛动物模型。对BCP小鼠进行鞘内置管,连续7天注射吗啡诱导耐受产生。von Frey纤维丝评估小鼠左后足50%机械刺激缩足反射阈值(mechanical withdrawal threshold,MWT)、热板法评估热缩足潜伏期阈值(thermal withdrawal threshold,TWL)。给药第7天后取L3~L5节段脊髓,使用免疫组化实验和Western Blotting(WB)方法检测小鼠脊髓Mas相关基因C受体(Mas-related gene C receptor,MrgC)的表达。通过对吗啡耐受的BCP小鼠鞘内注射右美托咪定,探索其可能的机制。结果:自建模后第7天起,BCP小鼠左后足50%MWT显著下降,TWL明显缩短(P<0.05)。建模后第14天起鞘内给予吗啡,给药的第1~4天,BCP小鼠左后足50%MWT明显上升,TWL明显增加(P<0.05),给药的第5天开始50%MWT逐渐下降,TWL明显缩短。但预先鞘内给予右美托咪定后,给药的第1~7天BCP小鼠左后足50%MWT持续增高,TWL持续延长(P<0.05),且免疫组化和WB结果显示MrgC表达明显增加。结论:右美托咪定可缓解BCP小鼠吗啡耐受的形成,这一作用可能与右美托咪定促进BCP小鼠脊髓MrgC表达和活化有关。展开更多
Cancer pain is one of the most common symptoms in patients with advanced cancer.In this study,we aimed to investigate the effects of the Mas-related gene C(MrgC)receptors on bone cancer pain.Mechanical withdrawal thre...Cancer pain is one of the most common symptoms in patients with advanced cancer.In this study,we aimed to investigate the effects of the Mas-related gene C(MrgC)receptors on bone cancer pain.Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were measured after the inoculation of Walker 256 mammary gland carcinoma cells into the tibia of adult Sprague-Dawley rats.The effects of MrgC receptor agonist bovine adrenal medulla 8-22(BAM8-22)on nociceptive behaviors were investigated after intrathecal injection on days 16 and 17.Glial fibrillary acidic protein(GFAP)-positive cells in the spinal dorsal cord,and calcitonin gene related peptide(CGRP)-,neuronal nitric oxide synthase(nNOS)-and IL-1β-positive neurons in the dorsal root ganglia(DRG)were examined by immunofluorescence staining.The expression of nNOS and IL-1βproteins in the spinal dorsal horn and the DRG was examined by Western blotting after treatment with(Tyr6)-γ2-MSH-6-12(MSH),which was another MrgC receptor agonist.The results showed that intrathecal injection of BAM8-22(30 nmol)attenuated mechanical allodynia in a rat model of bone cancer pain and the effects could last for about 60 min,and single administration of BAM8-22 for two consecutive days reduced mechanical allodynia by about half on the third day.Moreover,the number of GFAP-positive cells in the spinal dorsal horn,and the number of CGRP-,nNOS-and IL-1β-positive neurons in the DRG were decreased.Similarly,intrathecal administration of MSH(15 nmol)reduced the expression of nNOS and IL-1βin the spinal dorsal horn and the DRG.In conclusion,activation of MrgC receptors suppresses the activation of astrocytes in the spinal dorsal cord and the expression of CGRP,nNOS,and IL-1βin the spinal dorsal cord and/or DRG,which may underlie the inhibition of bone cancer pain.These findings provide a novel strategy for the treatment of bone cancer pain.展开更多
气体泄漏红外成像检测技术以其高效率遥测成像等显著优势成为气体泄漏检测的有效手段之一,针对此类系统探测能力的评价方法不全面的问题,通过改造MRTD测量靶标及方式,提出了适用于被动式气体红外成像检测系统性能的最小可分辨气体浓度MR...气体泄漏红外成像检测技术以其高效率遥测成像等显著优势成为气体泄漏检测的有效手段之一,针对此类系统探测能力的评价方法不全面的问题,通过改造MRTD测量靶标及方式,提出了适用于被动式气体红外成像检测系统性能的最小可分辨气体浓度MRGC(minimum resolvable gas concentration)评价方法,设计并搭建了MRGC性能测试系统,并以乙烯气体为检测目标进行了测试,测试结果与系统MRTD曲线的变化趋势具有较好的一致性.展开更多
Logging facies analysis is a significant aspect of reservoir description.In particular,as a commonly used method for logging facies identification,Multi-Resolution Graph-based Clustering(MRGC)can perform depth analysi...Logging facies analysis is a significant aspect of reservoir description.In particular,as a commonly used method for logging facies identification,Multi-Resolution Graph-based Clustering(MRGC)can perform depth analysis on multidimensional logging curves to predict logging facies.However,this method is very time-consuming and highly dependent on the initial parameters in the propagation process,which limits the practical application effect of the method.In this paper,an Adaptive Multi-Resolution Graph-based Clustering(AMRGC)is proposed,which is capable of both improving the efficiency of calculation process and achieving a stable propagation result.More specifically,the proposed method,1)presents a light kernel representative index(LKRI)algorithm which is proved to need less calculation resource than those kernel selection methods in the literature by exclusively considering those"free attractor"points;2)builds a Multi-Layer Perceptron(MLP)network with back propagation algorithm(BP)so as to avoid the uncertain results brought by uncertain parameter initializations which often happened by only using the K nearest neighbors(KNN)method.Compared with those clustering methods often used in image-based sedimentary phase analysis,such as Self Organizing Map(SOM),Dynamic Clustering(DYN)and Ascendant Hierarchical Clustering(AHC),etc.,the AMRGC performs much better without the prior knowledge of data structure.Eventually,the experimental results illustrate that the proposed method also outperformed the original MRGC method on the task of clustering and propagation prediction,with a higher efficiency and stability.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.31371124)the Ministry of Education's Industry-University Cooperation Collaborative Education Project(No.202101099013,201802136084).
文摘Cancer pain is one of the most common symptoms in patients with advanced cancer.In this study,we aimed to investigate the effects of the Mas-related gene C(MrgC)receptors on bone cancer pain.Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were measured after the inoculation of Walker 256 mammary gland carcinoma cells into the tibia of adult Sprague-Dawley rats.The effects of MrgC receptor agonist bovine adrenal medulla 8-22(BAM8-22)on nociceptive behaviors were investigated after intrathecal injection on days 16 and 17.Glial fibrillary acidic protein(GFAP)-positive cells in the spinal dorsal cord,and calcitonin gene related peptide(CGRP)-,neuronal nitric oxide synthase(nNOS)-and IL-1β-positive neurons in the dorsal root ganglia(DRG)were examined by immunofluorescence staining.The expression of nNOS and IL-1βproteins in the spinal dorsal horn and the DRG was examined by Western blotting after treatment with(Tyr6)-γ2-MSH-6-12(MSH),which was another MrgC receptor agonist.The results showed that intrathecal injection of BAM8-22(30 nmol)attenuated mechanical allodynia in a rat model of bone cancer pain and the effects could last for about 60 min,and single administration of BAM8-22 for two consecutive days reduced mechanical allodynia by about half on the third day.Moreover,the number of GFAP-positive cells in the spinal dorsal horn,and the number of CGRP-,nNOS-and IL-1β-positive neurons in the DRG were decreased.Similarly,intrathecal administration of MSH(15 nmol)reduced the expression of nNOS and IL-1βin the spinal dorsal horn and the DRG.In conclusion,activation of MrgC receptors suppresses the activation of astrocytes in the spinal dorsal cord and the expression of CGRP,nNOS,and IL-1βin the spinal dorsal cord and/or DRG,which may underlie the inhibition of bone cancer pain.These findings provide a novel strategy for the treatment of bone cancer pain.
基金supported by the National Natural Science Foundation of China(No.30070279)the Natural Science Foundation of Fujian Province,China(No.2014J01305)the Educational Commission of Fujian Province,China(No.JA14072)
基金supported by the National Natural Science Foundation of China (No. 31371124)the Natural Science Foundation of Fujian Province, China (No. 2014J01305)2018 “Xiyuanjiang Scholars” Support Program of College of Life Sciences, Fujian Normal University (No. FZSKG2018005)
文摘气体泄漏红外成像检测技术以其高效率遥测成像等显著优势成为气体泄漏检测的有效手段之一,针对此类系统探测能力的评价方法不全面的问题,通过改造MRTD测量靶标及方式,提出了适用于被动式气体红外成像检测系统性能的最小可分辨气体浓度MRGC(minimum resolvable gas concentration)评价方法,设计并搭建了MRGC性能测试系统,并以乙烯气体为检测目标进行了测试,测试结果与系统MRTD曲线的变化趋势具有较好的一致性.
基金sponsored by the Science and Technology Project of CNPC(No.2018D-5010-16 and 2019D-3808)。
文摘Logging facies analysis is a significant aspect of reservoir description.In particular,as a commonly used method for logging facies identification,Multi-Resolution Graph-based Clustering(MRGC)can perform depth analysis on multidimensional logging curves to predict logging facies.However,this method is very time-consuming and highly dependent on the initial parameters in the propagation process,which limits the practical application effect of the method.In this paper,an Adaptive Multi-Resolution Graph-based Clustering(AMRGC)is proposed,which is capable of both improving the efficiency of calculation process and achieving a stable propagation result.More specifically,the proposed method,1)presents a light kernel representative index(LKRI)algorithm which is proved to need less calculation resource than those kernel selection methods in the literature by exclusively considering those"free attractor"points;2)builds a Multi-Layer Perceptron(MLP)network with back propagation algorithm(BP)so as to avoid the uncertain results brought by uncertain parameter initializations which often happened by only using the K nearest neighbors(KNN)method.Compared with those clustering methods often used in image-based sedimentary phase analysis,such as Self Organizing Map(SOM),Dynamic Clustering(DYN)and Ascendant Hierarchical Clustering(AHC),etc.,the AMRGC performs much better without the prior knowledge of data structure.Eventually,the experimental results illustrate that the proposed method also outperformed the original MRGC method on the task of clustering and propagation prediction,with a higher efficiency and stability.
