AIM: To assess the functional status and etiology of liver cirrhosis by quantitative 31p magnetic resonance spectroscopy (MRS).METHODS: A total of 80 patients with liver cirrhosis of different etiology and functional ...AIM: To assess the functional status and etiology of liver cirrhosis by quantitative 31p magnetic resonance spectroscopy (MRS).METHODS: A total of 80 patients with liver cirrhosis of different etiology and functional status described by Child-Pugh score were examined and compared to 11 healthy volunteers. MR examination was performed on a 1.5 T imager using a 1H/31P surface coil by the 2D chemical shift imaging technique.Absolute concentrations of phosphomonoesters (PME),phosphodiesters (PDE), inorganic phosphate (Pi) and adenosine triphosphate (ATP) were measured.RESULTS: MRS changes reflected the degree of liver dysfunction in all the patients as well as in individual etiological groups. The most important change was a decrease of PDE. It was possible to distinguish alcoholic,viral and cholestatic etiologies based on MR spectra.Alcoholic and viral etiology differed in PDE (alcoholic,viral, controls: 6.5±2.3, 6.5±3.1, 10.8±2.7 mmol/L,P＜0.001) and ATP (alcoholic, viral, controls: 2.9±0.8, 2.8±0.9, 3.7±1.0 mmol/L, P＜0.01) from the control group.Unlike viral etiology, patients with alcoholic etiology also differed in Pi (alcoholic, controls: 1.2±0.4, 1.6±0.6mmol/L, P＜0.05) from controls. No significant changes were found in patients with cholestatic disease and controls; nevertheless, this group differed from both alcoholic and viral groups (cholestatic, alcoholic, viral: 9.4±2.7, 6.5±2.3, 6.5±3.1 mmol/L, P＜0.005) in PDE.CONCLUSION: 31p MRS can significantly help in noninvasive separation of different etiological groups leading to liver cirrhosis. In addition, MRS changes reflect functional liver injury.展开更多
AIM： To evaluate the effect of an intravenous bolus of mannitol in altering brain metabolites, brain water content, brain parenchyma volume, cerebrospinal fluid （CSF） volume and clinical signs in controls and in pa...AIM： To evaluate the effect of an intravenous bolus of mannitol in altering brain metabolites, brain water content, brain parenchyma volume, cerebrospinal fluid （CSF） volume and clinical signs in controls and in patients with acute liver failure （ALF） and acute- on-chronic liver failure （ACLF）, by comparing changes in conventional magnetic resonance imaging （MRI）, in vivo proton magnetic resonance spectroscopy （PMRS） and diffusion tensor imaging （DTI） before and after its infusion.METHODS： Five patients each with ALF and ACLF in grade 3 or 4 hepatic encephalopathy and with clinical signs of raised intracranial pressure were studied along with five healthy volunteers. After baseline MRI, an intravenous bolus of 20% mannitol solution was given over 10 min in controls as well as in patients with ALF and ACLE Repeat MRI for the same position was acquired 30 rnin after completing the rnannitol injection. RESULTS： No statistically significant difference was observed between controls and patients with ALF and ACLF in metabolite ratios, DTI metrics and brain volume or CSF volume following 45 rain of mannitol infusion. There was no change in clinical status at the end of post-mannitol imaging. CONCLUSION： The osmotic effect of mannitol did not result in significant reduction of brain water content, alteration in metabolite ratios or any change in the clinical status of these patients during or within 45 min of mannitol infusion.展开更多
Objective： To evaluate the clinical application of proton magnetic resonance spectroscopy （1HMRS） in patients with radiotherapy treated intracranial tumors. Methods： Forty patients with intracranial tumors underwe...Objective： To evaluate the clinical application of proton magnetic resonance spectroscopy （1HMRS） in patients with radiotherapy treated intracranial tumors. Methods： Forty patients with intracranial tumors underwent multivoxel 1HMRS examination before and after radiotherapy. The concentrations of N-acetyl aspartate （NAA）, choline （Cho） and creatine （Cr） were obtained both in the tumors and the contralateral normal brain regions, The ratios of NANCr, Cho/Cr and Cho/NAA were calculated at the same time and follow-up one year. Results： （1） After radiotherapy, tumors inhibited by radiation had decreased Cho, NAA and Cr on proton MRS. Some cases showed necrotic wave. （2） During the one year follow-up, local tumor recurred in 8 cases and their Cho and Cho/NAA increased high again. Other cases without recurrence, HMRS showed no change. Conclusion： Multivoxel proton MR spectroscopy is available for study of tumor metabolites after radiotherapy and it is a valuable method in the evaluation of radiotherapy treated tumors,展开更多
Background:Classification of breast cancer based on gene expression has emerged as the standard approach in its management,owing to the distinct prognoses and treatment responses observed among different subtypes.The ...Background:Classification of breast cancer based on gene expression has emerged as the standard approach in its management,owing to the distinct prognoses and treatment responses observed among different subtypes.The aim of this study was to prospectively assess the imaging features of the molecular subtypes of breast cancer using multiparametric magnetic resonance imaging(mMRI)with the combined assessment of dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI),diffusion-weighted imaging(DWI),and MR spectroscopy(MRS).Methods:This was a prospective observational single-center cohort study,which included women with BI-RADS 4−5 lesions on mammography/ultrasound(US)who subsequently underwent 1.5 T MRI(encompassing DCE-MRI,DWI,and MRS).The histological subtypes of breast cancer were assessed.Estrogen receptor(ER),progesterone receptor(PR),Ki-67 status,and human epidermal growth receptor-2(HER2)expression,assessed by immunohistochemistry(IHC),defined four molecular subtypes:luminal A,luminal B,HER2-enriched(Her2en),and triple-negative breast carcinoma(TNBC).Statistical associations between the four molecular subtypes and MRI features were investigated.Results:Fifty patients were included in the study.Circumscribed margins were significantly correlated with triple-negative tumors compared to others(78%versus 6%,p<0.001).Spiculated margins were observed in nontriple negative tumors.Rim enhancement was significantly correlated to triple-negative tumors compared to all other subtypes(71.4%versus 25%,p=0.035).Mean apparent diffusion coefficient(ADC)values were significantly lower for luminal subtypes compared to non-luminal subtypes(p<0.001).The total choline(tCho)signal-to-noise ratio(SNR)was higher in triple-negative tumors.A combined algorithm using DCE-MRI,DWI,and MRS can predict TNBC and Her2en with specificity of 86.6%and 100%,respectively,and sensitivity of 100%and 85.37%,respectively.Conclusion:The combination of mMRI with DCE-MRI,DWI,and MRS can accurately differentiate the molecular subtypes of breast carcinoma.展开更多
In this study,we systematically tested the hypothesis that during the critical developmental period of adolescence,on a macro scale,the concentrations of major excitatory and inhibitory neurotransmitters(glutamate/glu...In this study,we systematically tested the hypothesis that during the critical developmental period of adolescence,on a macro scale,the concentrations of major excitatory and inhibitory neurotransmitters(glutamate/glutamine andγ‑aminobutyric acid[GABA])in the dorsal and ventral lateral prefrontal cortex are associated with the brain’s functional connectivity and an individual’s psychopathology.Neurotransmitters were measured via magnetic resonance spectroscopy while functional connectivity was measured with resting-state fMRI(n=121).Seed-based and network-based analyses revealed associations of neurotransmitter concentrations and functional connectivities between regions/networks that are connected to prefrontal cortices via structural connections that are thought to be under dynamic development during adolescence.These regions tend to be boundary areas between functional networks.Furthermore,several connectivities were found to be associated with individual’s levels of internalizing psychopathology.These findings provide insights into specific neurochemical mechanisms underlying the brain’s macroscale functional organization,its development during adolescence,and its potential associations with symptoms associated with internalizing psychopathology.展开更多
AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy.
Proton magnetic resonance spectroscopy (1H MRS) can provide specific biochemical information within breast lesions and the elevated composite choline concentration as a useful diagnostic tool has been used to distingu...Proton magnetic resonance spectroscopy (1H MRS) can provide specific biochemical information within breast lesions and the elevated composite choline concentration as a useful diagnostic tool has been used to distinguish malignant from benign breast lesions, early evaluate response to therapy and predict prognosis. However, several obstacles including poor spatial resolution, low signal-to-noise ratio (SNR), long acquisition time and the difficulty of "extra" lipid suppression may have a negative impact on the routine application of in vivo 1H MRS for human breast cancer. At present, optimization H MRS methodology for breast studies has been performed.展开更多
We report a case of a 71 -year- old female with trichoblastic fibroma, a rare benign tumor of the skin, found in the left breast, associated with an invasive contralateral breast cancer. On clinical examination, a sol...We report a case of a 71 -year- old female with trichoblastic fibroma, a rare benign tumor of the skin, found in the left breast, associated with an invasive contralateral breast cancer. On clinical examination, a solitary, firm nodule was found in the subcutaneous tissue of the left breast with no changes in the overlying skin. Radiological examination showed disconcordant results. Conventional mammography and ultrasound suggested benign nature, while magnetic resonance mammography and spectroscopy raised the presumption of the malignant nature of the lesion. After performing excisional biopsy, the diagnosis of trichoblastic fibroma was established. Microscopically, it was composed of fibrous stroma, basaloid germs and strands and lace-like epithelial components, with no obvious connection with overlying epithelium or adjacent adnexal structures.展开更多
基金Supported by grant from Ministry of Health IGA 7853-3, and MZO 00023001, Czech Republic
文摘AIM: To assess the functional status and etiology of liver cirrhosis by quantitative 31p magnetic resonance spectroscopy (MRS).METHODS: A total of 80 patients with liver cirrhosis of different etiology and functional status described by Child-Pugh score were examined and compared to 11 healthy volunteers. MR examination was performed on a 1.5 T imager using a 1H/31P surface coil by the 2D chemical shift imaging technique.Absolute concentrations of phosphomonoesters (PME),phosphodiesters (PDE), inorganic phosphate (Pi) and adenosine triphosphate (ATP) were measured.RESULTS: MRS changes reflected the degree of liver dysfunction in all the patients as well as in individual etiological groups. The most important change was a decrease of PDE. It was possible to distinguish alcoholic,viral and cholestatic etiologies based on MR spectra.Alcoholic and viral etiology differed in PDE (alcoholic,viral, controls: 6.5±2.3, 6.5±3.1, 10.8±2.7 mmol/L,P＜0.001) and ATP (alcoholic, viral, controls: 2.9±0.8, 2.8±0.9, 3.7±1.0 mmol/L, P＜0.01) from the control group.Unlike viral etiology, patients with alcoholic etiology also differed in Pi (alcoholic, controls: 1.2±0.4, 1.6±0.6mmol/L, P＜0.05) from controls. No significant changes were found in patients with cholestatic disease and controls; nevertheless, this group differed from both alcoholic and viral groups (cholestatic, alcoholic, viral: 9.4±2.7, 6.5±2.3, 6.5±3.1 mmol/L, P＜0.005) in PDE.CONCLUSION: 31p MRS can significantly help in noninvasive separation of different etiological groups leading to liver cirrhosis. In addition, MRS changes reflect functional liver injury.
基金The Indian Council of Medical Research (Saksena S), IndiaNew Delhi (Nath K), IndiaThe National Institute of Mental Health,MH58284 and MH06595 (Thomas MA)
文摘AIM： To evaluate the effect of an intravenous bolus of mannitol in altering brain metabolites, brain water content, brain parenchyma volume, cerebrospinal fluid （CSF） volume and clinical signs in controls and in patients with acute liver failure （ALF） and acute- on-chronic liver failure （ACLF）, by comparing changes in conventional magnetic resonance imaging （MRI）, in vivo proton magnetic resonance spectroscopy （PMRS） and diffusion tensor imaging （DTI） before and after its infusion.METHODS： Five patients each with ALF and ACLF in grade 3 or 4 hepatic encephalopathy and with clinical signs of raised intracranial pressure were studied along with five healthy volunteers. After baseline MRI, an intravenous bolus of 20% mannitol solution was given over 10 min in controls as well as in patients with ALF and ACLE Repeat MRI for the same position was acquired 30 rnin after completing the rnannitol injection. RESULTS： No statistically significant difference was observed between controls and patients with ALF and ACLF in metabolite ratios, DTI metrics and brain volume or CSF volume following 45 rain of mannitol infusion. There was no change in clinical status at the end of post-mannitol imaging. CONCLUSION： The osmotic effect of mannitol did not result in significant reduction of brain water content, alteration in metabolite ratios or any change in the clinical status of these patients during or within 45 min of mannitol infusion.
基金a grant from the Medical Foundation of Wu Jieping(No.32067000501).
文摘Objective： To evaluate the clinical application of proton magnetic resonance spectroscopy （1HMRS） in patients with radiotherapy treated intracranial tumors. Methods： Forty patients with intracranial tumors underwent multivoxel 1HMRS examination before and after radiotherapy. The concentrations of N-acetyl aspartate （NAA）, choline （Cho） and creatine （Cr） were obtained both in the tumors and the contralateral normal brain regions, The ratios of NANCr, Cho/Cr and Cho/NAA were calculated at the same time and follow-up one year. Results： （1） After radiotherapy, tumors inhibited by radiation had decreased Cho, NAA and Cr on proton MRS. Some cases showed necrotic wave. （2） During the one year follow-up, local tumor recurred in 8 cases and their Cho and Cho/NAA increased high again. Other cases without recurrence, HMRS showed no change. Conclusion： Multivoxel proton MR spectroscopy is available for study of tumor metabolites after radiotherapy and it is a valuable method in the evaluation of radiotherapy treated tumors,
文摘Background:Classification of breast cancer based on gene expression has emerged as the standard approach in its management,owing to the distinct prognoses and treatment responses observed among different subtypes.The aim of this study was to prospectively assess the imaging features of the molecular subtypes of breast cancer using multiparametric magnetic resonance imaging(mMRI)with the combined assessment of dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI),diffusion-weighted imaging(DWI),and MR spectroscopy(MRS).Methods:This was a prospective observational single-center cohort study,which included women with BI-RADS 4−5 lesions on mammography/ultrasound(US)who subsequently underwent 1.5 T MRI(encompassing DCE-MRI,DWI,and MRS).The histological subtypes of breast cancer were assessed.Estrogen receptor(ER),progesterone receptor(PR),Ki-67 status,and human epidermal growth receptor-2(HER2)expression,assessed by immunohistochemistry(IHC),defined four molecular subtypes:luminal A,luminal B,HER2-enriched(Her2en),and triple-negative breast carcinoma(TNBC).Statistical associations between the four molecular subtypes and MRI features were investigated.Results:Fifty patients were included in the study.Circumscribed margins were significantly correlated with triple-negative tumors compared to others(78%versus 6%,p<0.001).Spiculated margins were observed in nontriple negative tumors.Rim enhancement was significantly correlated to triple-negative tumors compared to all other subtypes(71.4%versus 25%,p=0.035).Mean apparent diffusion coefficient(ADC)values were significantly lower for luminal subtypes compared to non-luminal subtypes(p<0.001).The total choline(tCho)signal-to-noise ratio(SNR)was higher in triple-negative tumors.A combined algorithm using DCE-MRI,DWI,and MRS can predict TNBC and Her2en with specificity of 86.6%and 100%,respectively,and sensitivity of 100%and 85.37%,respectively.Conclusion:The combination of mMRI with DCE-MRI,DWI,and MRS can accurately differentiate the molecular subtypes of breast carcinoma.
基金supported by NIMH grant R01105501(PI:MTB and BLK).
文摘In this study,we systematically tested the hypothesis that during the critical developmental period of adolescence,on a macro scale,the concentrations of major excitatory and inhibitory neurotransmitters(glutamate/glutamine andγ‑aminobutyric acid[GABA])in the dorsal and ventral lateral prefrontal cortex are associated with the brain’s functional connectivity and an individual’s psychopathology.Neurotransmitters were measured via magnetic resonance spectroscopy while functional connectivity was measured with resting-state fMRI(n=121).Seed-based and network-based analyses revealed associations of neurotransmitter concentrations and functional connectivities between regions/networks that are connected to prefrontal cortices via structural connections that are thought to be under dynamic development during adolescence.These regions tend to be boundary areas between functional networks.Furthermore,several connectivities were found to be associated with individual’s levels of internalizing psychopathology.These findings provide insights into specific neurochemical mechanisms underlying the brain’s macroscale functional organization,its development during adolescence,and its potential associations with symptoms associated with internalizing psychopathology.
基金Supported by Grants from the National Natural Science Foundation of China(Key program 30930027)Natural Science Foundation of Guangdong Province(No.8151503102000032)
文摘AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy.
文摘Proton magnetic resonance spectroscopy (1H MRS) can provide specific biochemical information within breast lesions and the elevated composite choline concentration as a useful diagnostic tool has been used to distinguish malignant from benign breast lesions, early evaluate response to therapy and predict prognosis. However, several obstacles including poor spatial resolution, low signal-to-noise ratio (SNR), long acquisition time and the difficulty of "extra" lipid suppression may have a negative impact on the routine application of in vivo 1H MRS for human breast cancer. At present, optimization H MRS methodology for breast studies has been performed.
文摘We report a case of a 71 -year- old female with trichoblastic fibroma, a rare benign tumor of the skin, found in the left breast, associated with an invasive contralateral breast cancer. On clinical examination, a solitary, firm nodule was found in the subcutaneous tissue of the left breast with no changes in the overlying skin. Radiological examination showed disconcordant results. Conventional mammography and ultrasound suggested benign nature, while magnetic resonance mammography and spectroscopy raised the presumption of the malignant nature of the lesion. After performing excisional biopsy, the diagnosis of trichoblastic fibroma was established. Microscopically, it was composed of fibrous stroma, basaloid germs and strands and lace-like epithelial components, with no obvious connection with overlying epithelium or adjacent adnexal structures.
