γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）....γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）. The terminal thiol groups （-SH） in the MPTS-MPTES SAM were in-situ oxidized into sulfonic acid groups （-SO3H） to endow the film with good chemisorption ability. Then rare earth （RE） （lanthanum-based） composite thin films were prepared by self-assembly technique based on the as-prepared SAM, taking advantage of the chemisorption ability of the sulfonic acid groups. Automatic force microscope （AFM）, X-ray photoelectron spectrometry （XPS）, contact angle measurement and ellipsometer were used to characterize MPTS-MPTES/RE composite films. The macrofriction and wear behaviors of the films sliding against an AISI-52100 steel ball were examined on a unidirectional friction and wear tester, and the worn surface morphologies were observed on an AFM. The results showed that MPTS-MPTES/RE films had a low friction coefficient （0.09） and a long wear life （5980 sliding pass） at a light load （50 mN）. It indicated that the superior tribological properties of the MPTS-MPTES/RE composite films were attributed to the special characteristic of RE elements, the mobility of the films and good bonding strength.展开更多
分析微柱凝胶试验(Microtubes Gel Test,MGT)免疫检测技术在交叉配血+血型鉴定中的作用效果。方法 南通市海门区人民医院输血科于2021年2月~2024年7月时间段选择50例输血患者进行回顾性研究,检测过程中根据输血需求选择50例无偿献血者...分析微柱凝胶试验(Microtubes Gel Test,MGT)免疫检测技术在交叉配血+血型鉴定中的作用效果。方法 南通市海门区人民医院输血科于2021年2月~2024年7月时间段选择50例输血患者进行回顾性研究,检测过程中根据输血需求选择50例无偿献血者提供的血液样本。交叉配血行MPT+MGT方法,血型鉴定行MGT+盐水试管方法,分析交叉配血+血型鉴定情况。结果 MGT与盐水试管血型鉴定正反定型相符结果无明显组间对比差异P>0.05。MGT交叉配血成功率更高P<0.05。结论 交叉配血、血型鉴定工作开展过程中应用微柱凝胶试验(MGT)免疫检测技术精准度高,显著提高输血科工作质量,应用价值大。展开更多
In the last decade,space solar power satellites(SSPSs)have been conceived to support net-zero carbon emissions and have attracted considerable attention.Electric energy is transmitted to the ground via a microwave pow...In the last decade,space solar power satellites(SSPSs)have been conceived to support net-zero carbon emissions and have attracted considerable attention.Electric energy is transmitted to the ground via a microwave power beam,a technology known as microwave power transmission(MPT).Due to the vast transmission distance of tens of thousands of kilometers,the power transmitting antenna array must span up to 1 kilometer in diameter.At the same time,the size of the rectifying array on the ground should extend over a few kilometers.This makes the MPT system of SSPSs significantly larger than the existing aerospace engineering system.To design and operate a rational MPT system,comprehensive optimization is required.Taking the space MPT system engineering into consideration,a novel multi-objective optimization function is proposed and further analyzed.The multi-objective optimization problem is modeled mathematically.Beam collection efficiency(BCE)is the primary factor,followed by the thermal management capability.Some tapers,designed to solve the conflict between BCE and the thermal problem,are reviewed.In addition to these two factors,rectenna design complexity is included as a functional factor in the optimization objective.Weight coefficients are assigned to these factors to prioritize them.Radiating planar arrays with different aperture illumination fields are studied,and their performances are compared using the multi-objective optimization function.Transmitting array size,rectifying array size,transmission distance,and transmitted power remaine constant in various cases,ensuring fair comparisons.The analysis results show that the proposed optimization function is effective in optimizing and selecting the MPT system architecture.It is also noted that the multi-objective optimization function can be expanded to include other factors in the future.展开更多
目的采用生物信息学方法分析、预测结核分枝杆菌Rv1980c基因编码蛋白MPT64的结构和功能。方法通过NCBI网站获取Rv1980c基因的基本信息;使用Proteparam、ProtScale和ProtCompB软件预测MPT64蛋白的理化性质和亚细胞定位;使用SignalP Serve...目的采用生物信息学方法分析、预测结核分枝杆菌Rv1980c基因编码蛋白MPT64的结构和功能。方法通过NCBI网站获取Rv1980c基因的基本信息;使用Proteparam、ProtScale和ProtCompB软件预测MPT64蛋白的理化性质和亚细胞定位;使用SignalP Server v.4.0、TMHMM Server v.2.0、NetNGlyc Server 1.0和Net Phos Server v.3.1对MPT64蛋白的信号肽、跨膜结构、糖基化及磷酸化位点进行分析;使用SOPMA和I-TASSER预测MPT64蛋白的二级结构并对该蛋白的三级结构分子模型进行构建;使用BepiPred和NetMHCpan-4.1、NetMHCIIpan-4.0对MPT64蛋白的抗原表位进行分析预测;使用VaxiJen、ToxIBT对预测出的表位进行抗原相似性及毒性筛选;使用STRING数据库预测MPT64的相互作用蛋白及其相关功能。结果Rv1980c编码的MPT64蛋白由228个氨基酸组成,其分子式为C_(1115)H_(1729)N_(285)O_(342)S_(8),理论等电点为4.84,平均亲水性系数为-2.322,不稳定性指数28.73,预测其为亲水性蛋白;亚细胞可能定位于细胞外。MPT64蛋白可能存在一个跨膜结构,可能存在一个信号肽、两个糖基化位点和25个磷酸化位点。其二级结构包含28.07%的a-螺旋(Hh),18.86%的β-折叠(Ee),5.26%的β-转角(Tt),47.81%无规则卷曲(Cc)。MPT64蛋白含有2个B细胞优势表位,12个T细胞优势表位;预测其互作蛋白为FBPB、ESXA、MPT63、ESXB、NrdF1、FBPA、Pst S1、APA、CFP21、MPT70。结论成功对MPT64蛋白进行了生物信息学分析与结构功能预测,为MPT64的进一步研究奠定基础。展开更多
基金supported by the National Natural Science Foundation of China (50475023)
文摘γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）. The terminal thiol groups （-SH） in the MPTS-MPTES SAM were in-situ oxidized into sulfonic acid groups （-SO3H） to endow the film with good chemisorption ability. Then rare earth （RE） （lanthanum-based） composite thin films were prepared by self-assembly technique based on the as-prepared SAM, taking advantage of the chemisorption ability of the sulfonic acid groups. Automatic force microscope （AFM）, X-ray photoelectron spectrometry （XPS）, contact angle measurement and ellipsometer were used to characterize MPTS-MPTES/RE composite films. The macrofriction and wear behaviors of the films sliding against an AISI-52100 steel ball were examined on a unidirectional friction and wear tester, and the worn surface morphologies were observed on an AFM. The results showed that MPTS-MPTES/RE films had a low friction coefficient （0.09） and a long wear life （5980 sliding pass） at a light load （50 mN）. It indicated that the superior tribological properties of the MPTS-MPTES/RE composite films were attributed to the special characteristic of RE elements, the mobility of the films and good bonding strength.
文摘In the last decade,space solar power satellites(SSPSs)have been conceived to support net-zero carbon emissions and have attracted considerable attention.Electric energy is transmitted to the ground via a microwave power beam,a technology known as microwave power transmission(MPT).Due to the vast transmission distance of tens of thousands of kilometers,the power transmitting antenna array must span up to 1 kilometer in diameter.At the same time,the size of the rectifying array on the ground should extend over a few kilometers.This makes the MPT system of SSPSs significantly larger than the existing aerospace engineering system.To design and operate a rational MPT system,comprehensive optimization is required.Taking the space MPT system engineering into consideration,a novel multi-objective optimization function is proposed and further analyzed.The multi-objective optimization problem is modeled mathematically.Beam collection efficiency(BCE)is the primary factor,followed by the thermal management capability.Some tapers,designed to solve the conflict between BCE and the thermal problem,are reviewed.In addition to these two factors,rectenna design complexity is included as a functional factor in the optimization objective.Weight coefficients are assigned to these factors to prioritize them.Radiating planar arrays with different aperture illumination fields are studied,and their performances are compared using the multi-objective optimization function.Transmitting array size,rectifying array size,transmission distance,and transmitted power remaine constant in various cases,ensuring fair comparisons.The analysis results show that the proposed optimization function is effective in optimizing and selecting the MPT system architecture.It is also noted that the multi-objective optimization function can be expanded to include other factors in the future.
文摘目的采用生物信息学方法分析、预测结核分枝杆菌Rv1980c基因编码蛋白MPT64的结构和功能。方法通过NCBI网站获取Rv1980c基因的基本信息;使用Proteparam、ProtScale和ProtCompB软件预测MPT64蛋白的理化性质和亚细胞定位;使用SignalP Server v.4.0、TMHMM Server v.2.0、NetNGlyc Server 1.0和Net Phos Server v.3.1对MPT64蛋白的信号肽、跨膜结构、糖基化及磷酸化位点进行分析;使用SOPMA和I-TASSER预测MPT64蛋白的二级结构并对该蛋白的三级结构分子模型进行构建;使用BepiPred和NetMHCpan-4.1、NetMHCIIpan-4.0对MPT64蛋白的抗原表位进行分析预测;使用VaxiJen、ToxIBT对预测出的表位进行抗原相似性及毒性筛选;使用STRING数据库预测MPT64的相互作用蛋白及其相关功能。结果Rv1980c编码的MPT64蛋白由228个氨基酸组成,其分子式为C_(1115)H_(1729)N_(285)O_(342)S_(8),理论等电点为4.84,平均亲水性系数为-2.322,不稳定性指数28.73,预测其为亲水性蛋白;亚细胞可能定位于细胞外。MPT64蛋白可能存在一个跨膜结构,可能存在一个信号肽、两个糖基化位点和25个磷酸化位点。其二级结构包含28.07%的a-螺旋(Hh),18.86%的β-折叠(Ee),5.26%的β-转角(Tt),47.81%无规则卷曲(Cc)。MPT64蛋白含有2个B细胞优势表位,12个T细胞优势表位;预测其互作蛋白为FBPB、ESXA、MPT63、ESXB、NrdF1、FBPA、Pst S1、APA、CFP21、MPT70。结论成功对MPT64蛋白进行了生物信息学分析与结构功能预测,为MPT64的进一步研究奠定基础。
