Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powde...Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP) addressed on characterization of the comprising stilbenes and effectiveness in longevity extension deserves intensive research. In this study, peanut kernels were subjected to germination and wound-stress in preparation of BPSP. The methanol extracts of BPSP were medium pressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLC recovered and followed by instrumental identification and biological activity determinations of the isolated stilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and both genders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 g BPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enriched quantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two new stilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’, 6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibited potent antioxidative and antiglycative activities and varied with structure-activity nature. Based on the resultant survival curves and average lifespans of both mouse models, basal diets supplemented with BPSP are effective to extend mouse longevity by a dose dependent manner. It is of merit to demonstrate that peanut kernels as a potent producer could be bio-elicited to biosynthesize a broad spectrum of bioactive stilbenes to prepare BPSP which is effective to extend mouse longevity as science-evidenced by the two long-term animal experiments.展开更多
Multiple pulmonary ground-glass nodules(GGNs),a typical clinical manifestation of multiple primary lung cancers(MPLCs),are of great significance for the early screening,diagnosis,and accurate treatment of lung cancer....Multiple pulmonary ground-glass nodules(GGNs),a typical clinical manifestation of multiple primary lung cancers(MPLCs),are of great significance for the early screening,diagnosis,and accurate treatment of lung cancer.Thus,the recent increase in the detection rate of multiple pulmonary GGNs has attracted much attention.However,compared with the more widely studied single GGNs,evaluating GGNs is more challenging because of the uncertainty of the etiology,difficult differential diagnosis,and lack of optimal management standards and guidelines.Most current solutions for multiple GGNs are based on the management experiences and principles of a single GGN.Therefore,it is necessary to obtain better understanding of multiple GGNs and to optimize the diagnostic methods and treatments.Both the existing challenges and potential of new methods for diagnosing and treating multiple pulmonary GGNs are reviewed and discussed in this article,with the aim of providing a reference for the clinical management of this highly prevalent condition.展开更多
文摘Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP) addressed on characterization of the comprising stilbenes and effectiveness in longevity extension deserves intensive research. In this study, peanut kernels were subjected to germination and wound-stress in preparation of BPSP. The methanol extracts of BPSP were medium pressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLC recovered and followed by instrumental identification and biological activity determinations of the isolated stilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and both genders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 g BPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enriched quantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two new stilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’, 6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibited potent antioxidative and antiglycative activities and varied with structure-activity nature. Based on the resultant survival curves and average lifespans of both mouse models, basal diets supplemented with BPSP are effective to extend mouse longevity by a dose dependent manner. It is of merit to demonstrate that peanut kernels as a potent producer could be bio-elicited to biosynthesize a broad spectrum of bioactive stilbenes to prepare BPSP which is effective to extend mouse longevity as science-evidenced by the two long-term animal experiments.
文摘Multiple pulmonary ground-glass nodules(GGNs),a typical clinical manifestation of multiple primary lung cancers(MPLCs),are of great significance for the early screening,diagnosis,and accurate treatment of lung cancer.Thus,the recent increase in the detection rate of multiple pulmonary GGNs has attracted much attention.However,compared with the more widely studied single GGNs,evaluating GGNs is more challenging because of the uncertainty of the etiology,difficult differential diagnosis,and lack of optimal management standards and guidelines.Most current solutions for multiple GGNs are based on the management experiences and principles of a single GGN.Therefore,it is necessary to obtain better understanding of multiple GGNs and to optimize the diagnostic methods and treatments.Both the existing challenges and potential of new methods for diagnosing and treating multiple pulmonary GGNs are reviewed and discussed in this article,with the aim of providing a reference for the clinical management of this highly prevalent condition.
