Fuel station drive-offs,wherein the drivers simply drive off without paying,are a major issue in the UK(United Kingdom)due to rising fuel costs and financial hardships.The phenomenon has increased greatly over the las...Fuel station drive-offs,wherein the drivers simply drive off without paying,are a major issue in the UK(United Kingdom)due to rising fuel costs and financial hardships.The phenomenon has increased greatly over the last few years,with reports indicating a substantial increase in such events in the major cities.Traditional prevention measures such as Avutec and Driveoffalert rely primarily on expensive infrastructure and blacklisted databases.Such systems typically involve costly camera installation andmaintenance and are consequently out of the budget of small fuel stations.These conventional approaches also fall short regarding real-time recognition,particularly regarding first-time impostors using fictitious plates,which represent an increasingly significant proportion of such forgery.This research presents an AI(Artificial Intelligence)-driven detection system using the MOT(Ministry of Transport)History API(Application Programming Interface)to scan in real-time at gas stations to recognize and prevent such fraud.The system integrates various state-of-the-art technologies to offer a foolproof system.Using the latestYOLO(YouOnly Look Once)model to recognize number plates and EasyOCR(Optical Character Recognition)to recognize characters,the system correctly reads license plates in various environmental conditions like lighting,viewpoint,and weather conditions.This approach minimizes the utilization of expensive camera systems and employs cheaper ANPR(AutomaticNumber Plate Recognition)gear,availing existing installed surveillance cameras on filling stations.The system operates with a basic web-based application to notify operators of stolen vehicles in real-time,enabling them to react immediately.Real-world testing achieves 84%success with CCTV(Closed-Circuit Television)images,depicting its real-world applicability.The results indicate that the AI-driven solution offers a monumental leap compared to current practices,giving fuel stations a cost-effective and efficient means of reducing financial loss from drive-off incidents.展开更多
Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral ...Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral stroke, and neurodegenerative diseases. The earlier MOT results in better efficacy in animal models of urgent diseases such as ischemic stroke, and traumatic brain and spinal cord injuries. There is no long-term method to preserve mitochondria. Routine MOT procedure from cell growth to mitochondrial injection often takes serval weeks and is not satisfactory for urgent use cases. Hypothesis: Cryopreserved cells might be mitochondrial donors for MOT. Methods: We isolated mitochondria from cryopreserved human fibroblasts and mesenchymal stem cells (MSCs) in cell banks and compared the mitochondrial viability and transplantation with the mitochondria from fresh cells. Key findings: We found that mitochondria from fresh and cryopreserved cells are comparable in mitochondrial viability and transplantation. We also obtained data showing that mitochondria of fibroblasts and MSCs had similar membrane potential and transfer ability, but MSC’s mitochondria had higher ATP content than fibroblast’s mitochondria. In addition, oxygen consumption rates (OCRs) were higher in MSC’s mitochondria compared to fibroblast’s mitochondria and did not change between fresh and frozen cells. Conclusion: Cryopreserved fibroblasts and MSCs are alternative mitochondrial donors for MOT to fresh cells. MSCs could provide higher ATP-produced mitochondria than fibroblasts.展开更多
The purpose of this study was to observe record and compare the children＇s of early childhood performance of motor skills of different nationalities from Greece, Albania and Sweden. Additionally to investigate differ...The purpose of this study was to observe record and compare the children＇s of early childhood performance of motor skills of different nationalities from Greece, Albania and Sweden. Additionally to investigate differences in motor performance between boys and girls and between age groups. The survey was conducted in the school years 2013-2014 and 2014-2015, and took place in the flame of the student exchange program ERASMUS internships of Preschool Education, University of loannina. The sample consisted of 369 infants （187 boys, 182 girls） aged 66 ± 7 months. The sample was selected according to the access that the team had in nursery schools of Ioannina （N1 = 133）, in Dervitsani （N2 = 131） Albania and Gothenburg （N2 = 105） of Sweden. The array of 18 different motor activities for children aged 4-6 years old was used to investigate the toddlers＇ degree of movement performance. They were used the manufacturer＇s instructions for the degree of movement performance and classification of the sample in different categories, while a descriptive statistics and analysis of variance （Three Way ANOVA） took place for sex factors, age and region of origin. The results showed that although there were differences in rates distributions and averages, there were no significant differences either between children from the three countries or between boys and girls, however, there were in the age groups with older children who achieved better rates.展开更多
文摘Fuel station drive-offs,wherein the drivers simply drive off without paying,are a major issue in the UK(United Kingdom)due to rising fuel costs and financial hardships.The phenomenon has increased greatly over the last few years,with reports indicating a substantial increase in such events in the major cities.Traditional prevention measures such as Avutec and Driveoffalert rely primarily on expensive infrastructure and blacklisted databases.Such systems typically involve costly camera installation andmaintenance and are consequently out of the budget of small fuel stations.These conventional approaches also fall short regarding real-time recognition,particularly regarding first-time impostors using fictitious plates,which represent an increasingly significant proportion of such forgery.This research presents an AI(Artificial Intelligence)-driven detection system using the MOT(Ministry of Transport)History API(Application Programming Interface)to scan in real-time at gas stations to recognize and prevent such fraud.The system integrates various state-of-the-art technologies to offer a foolproof system.Using the latestYOLO(YouOnly Look Once)model to recognize number plates and EasyOCR(Optical Character Recognition)to recognize characters,the system correctly reads license plates in various environmental conditions like lighting,viewpoint,and weather conditions.This approach minimizes the utilization of expensive camera systems and employs cheaper ANPR(AutomaticNumber Plate Recognition)gear,availing existing installed surveillance cameras on filling stations.The system operates with a basic web-based application to notify operators of stolen vehicles in real-time,enabling them to react immediately.Real-world testing achieves 84%success with CCTV(Closed-Circuit Television)images,depicting its real-world applicability.The results indicate that the AI-driven solution offers a monumental leap compared to current practices,giving fuel stations a cost-effective and efficient means of reducing financial loss from drive-off incidents.
文摘Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral stroke, and neurodegenerative diseases. The earlier MOT results in better efficacy in animal models of urgent diseases such as ischemic stroke, and traumatic brain and spinal cord injuries. There is no long-term method to preserve mitochondria. Routine MOT procedure from cell growth to mitochondrial injection often takes serval weeks and is not satisfactory for urgent use cases. Hypothesis: Cryopreserved cells might be mitochondrial donors for MOT. Methods: We isolated mitochondria from cryopreserved human fibroblasts and mesenchymal stem cells (MSCs) in cell banks and compared the mitochondrial viability and transplantation with the mitochondria from fresh cells. Key findings: We found that mitochondria from fresh and cryopreserved cells are comparable in mitochondrial viability and transplantation. We also obtained data showing that mitochondria of fibroblasts and MSCs had similar membrane potential and transfer ability, but MSC’s mitochondria had higher ATP content than fibroblast’s mitochondria. In addition, oxygen consumption rates (OCRs) were higher in MSC’s mitochondria compared to fibroblast’s mitochondria and did not change between fresh and frozen cells. Conclusion: Cryopreserved fibroblasts and MSCs are alternative mitochondrial donors for MOT to fresh cells. MSCs could provide higher ATP-produced mitochondria than fibroblasts.
文摘The purpose of this study was to observe record and compare the children＇s of early childhood performance of motor skills of different nationalities from Greece, Albania and Sweden. Additionally to investigate differences in motor performance between boys and girls and between age groups. The survey was conducted in the school years 2013-2014 and 2014-2015, and took place in the flame of the student exchange program ERASMUS internships of Preschool Education, University of loannina. The sample consisted of 369 infants （187 boys, 182 girls） aged 66 ± 7 months. The sample was selected according to the access that the team had in nursery schools of Ioannina （N1 = 133）, in Dervitsani （N2 = 131） Albania and Gothenburg （N2 = 105） of Sweden. The array of 18 different motor activities for children aged 4-6 years old was used to investigate the toddlers＇ degree of movement performance. They were used the manufacturer＇s instructions for the degree of movement performance and classification of the sample in different categories, while a descriptive statistics and analysis of variance （Three Way ANOVA） took place for sex factors, age and region of origin. The results showed that although there were differences in rates distributions and averages, there were no significant differences either between children from the three countries or between boys and girls, however, there were in the age groups with older children who achieved better rates.
