We present a method of 3D image mosaicing for real 3D representation of roadside buildings, and implement a Web-based interactive visualization environment for the 3D video mosaics created by 3D image mosaicing. The 3...We present a method of 3D image mosaicing for real 3D representation of roadside buildings, and implement a Web-based interactive visualization environment for the 3D video mosaics created by 3D image mosaicing. The 3D image mo- saicing technique developed in our previous work is a very powerful method for creating textured 3D-GIS data without excessive data processing like the laser or stereo system. For the Web-based open access to the 3D video mosaics, we build an interactive visualization environment using X3D, the emerging standard of Web 3D. We conduct the data preprocessing for 3D video mosaics and the X3D modeling for textured 3D data. The data preprocessing includes the conversion of each frame of 3D video mosaics into concatenated image files that can be hyperlinked on the Web. The X3D modeling handles the representation of concatenated images using necessary X3D nodes. By employing X3D as the data format for 3D image mosaics, the real 3D representation of roadside buildings is extended to the Web and mobile service systems.展开更多
Although the unique organization of vertebrate cone mosaics was first described long ago,both their underlying molecular basis and physiological significance are largely unknown.Here,we demonstrate that Crumbs protein...Although the unique organization of vertebrate cone mosaics was first described long ago,both their underlying molecular basis and physiological significance are largely unknown.Here,we demonstrate that Crumbs proteins,the key regulators of epithelial apical polarity,establish the planar cellular polarity of photoreceptors in zebrafish.Via heterophilic Crb2a-Crb2b interactions,the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors.The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors,thereby stabilizing the geometric organization of cone mosaics.Notably,loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation.These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness.展开更多
This paper investigated several approaches to remapping and combining multiple-radar reflectivity fields onto a unified 3D Cartesian grid with high spatial and temporal resolutions, and analyzed systematic observation...This paper investigated several approaches to remapping and combining multiple-radar reflectivity fields onto a unified 3D Cartesian grid with high spatial and temporal resolutions, and analyzed systematic observation differences among multiple radars. The remapping approaches were evaluated by inspecting the spatial consistency of the reflectivity fields on vertical and horizontal cross sections on the equidistant line of radars, and the intensity change of 1-h accumulated precipitation before and after interpolation. The combining approaches were evaluated by continuity examination. The results show that for remapping schemes, the vertical interpolation with nearest neighbor on the range-azimuth plane is the most reasonable scheme that provides consecutive reflectivity fields and retains the high-resolution structure comparable to that of the raw data; for multiple-radar data mosaics, the distance-exponential-weighted mean scheme provides spatially consistent reflectivity mosaics. The mosaics can mitigate various problems caused by the radar beam geometry such as the cone of silence.展开更多
Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic v...Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic virus(TuMV)is a major pathogen affecting Chinese cabbages,leading to severe yield losses(Li et al.,2019).Traditional control measures have shown limited efficacy,and the long-term use of chemical pesticides has led to significant issues such as environmental pollution and pathogen resistance(Samara et al.,2021;Lu et al.,2022).Biologicallyderived pesticides have garnered considerable attention owing to their eco-friendly attributes(Ayilara et al.,2023).γ-Aminobutyric acid(GABA),initially discovered in potato tubers,has been proven to regulate immune responses and enhance resistance to fungal and bacterial pathogens by modulating reactive oxygen species and stress-related hormone signals(Tarkowski et al.,2020;Wang et al.,2025).But biologically-derived agents typically face challenges such as large particle size and instability,which limit their practical application and bioavailability(Daraban et al.,2023).展开更多
Assisted hatching(AH)is commonly performed before trophectoderm(TE)biopsy for preimplantation genetic testing for aneuploidy(PGT-A),yet whether AH timing influences the detection of chromosomal mosaicism remains uncer...Assisted hatching(AH)is commonly performed before trophectoderm(TE)biopsy for preimplantation genetic testing for aneuploidy(PGT-A),yet whether AH timing influences the detection of chromosomal mosaicism remains uncertain.Beyond timing,procedural choices in PGT-A must balance diagnostic yield against potential harm from additional handling(e.g.,repeat TE biopsy or cryopreservation steps),as underscored by a recent systematic review and meta-analysis[1].展开更多
China and Nepal deepen cooperation to promote traditional medicine across the Himalayan region.THE Himalayan region features a rich mosaic of cultures that are distinct yet deeply intertwined.Traditional medicine,a co...China and Nepal deepen cooperation to promote traditional medicine across the Himalayan region.THE Himalayan region features a rich mosaic of cultures that are distinct yet deeply intertwined.Traditional medicine,a common treasure of human civilization,has deep roots in this region.展开更多
Jute is the golden fiber crop of Bangladesh. Viral and Nemic diseases are the major threats to fiber crops, and the fiber yield decreases drastically as the disease infection increases. An experiment was conducted to ...Jute is the golden fiber crop of Bangladesh. Viral and Nemic diseases are the major threats to fiber crops, and the fiber yield decreases drastically as the disease infection increases. An experiment was conducted to screen and evaluate ten Jute and allied fiber varieties i.e. BJRI Kenaf-4, BJRI Kenaf-3, HS-95 (Kenaf), HS-24 (Mesta pat), HC-2 (Kenaf), BJRI Deshi pat-8, BJRI Deshi pat-7, BJRI Deshi pat-5, BJRI Deshi pat-6 and CVL-1 against mosaic virus and root-knot nematode disease. It was done in RCBD design with three replications at Sher-e-Bangla Agricultural University (SAU) central farm, Dhaka-1207, from June 2018 to March 2019. In the case of viral infection, variety 4 (HS-24 (Mesta pat)) showed maximum virus incidence (23.11%), whereas variety 2 (BJRI Kenaf-3) had no virus evidence. The most susceptible nematode-infected variety was found in varieties 5 (HC-2 (Kenaf)) and 9 (BJRI Deshi pat-6), which showed 80% incidence, whereas variety 2 (BJRI Kenaf-3) was highly resistance. The growth and yield results showed a significant (0.05%) reduction of plant height (cm) and fiber weight (t/ha) with an increase in the percentage of virus and nematode incidence against all varieties. The highest fiber weight (1.47 t/ha) and the lowest viral (0%) and nemic infection (0%) were found in variety 2 (BJRI Kenaf-3), which showed better performance compared to other varieties. These results revealed that variety 2 (BJRI Kenaf-3) had the best variety in the present study.展开更多
Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and t...Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.展开更多
Using nine ice-tethered buoys deployed across the marginal ice zone(MIZ)and pack ice zone(PIZ)north of the Laptev Sea during the expedition of the Multidisciplinary drifting Observatory for the Study of Arctic Climate...Using nine ice-tethered buoys deployed across the marginal ice zone(MIZ)and pack ice zone(PIZ)north of the Laptev Sea during the expedition of the Multidisciplinary drifting Observatory for the Study of Arctic Climate(MOSAiC)in 2019-2020,we characterized the spatiotemporal variations in sea ice kinematics and deformation between October 2019 and July 2020 in the Transpolar Drift(TPD).From October to November,the buoys were in the upstream area of the TPD;spatial variations of deformation rates were significantly correlated with initial ice thickness(R=−0.84,P<0.05).From December 2019 to March 2020,the buoys were in the high Arctic and the ice cover was consolidated;heterogeneity in ice kinematics as measured across the buoys reduced by 65%.From April to May 2020,the buoys were in the downstream TPD;amplified spatial variations in ice kinematics were observed.This is because two buoys had drifted over the shallow waters north of Svalbard earlier;trajectory-stretching exponents derived from the data from these two buoys indicate deformation rates(10.6 d^(−1))that were about twice those in the deep basin(4.2 d^(−1)).By June 2020,a less consolidated ice pack and enhanced tidal forcing in the Fram Strait MIZ resulted in ice deformation with a semi-diurnal power spectral density of>0.25 d^(−1),which is about 1.5 times that in PIZ.Therefore,in both the upstream and downstream regions of the TPD,the transition between the MIZ and the PIZ contributes to the spatial and seasonal variations of sea ice motion and deformation.The results from this study can be used to support the characterization of the momentum balance and influencing factors during the ice advection along the TPD,which is a crucial corridor for Arctic sea ice outflow to the north Atlantic Ocean.展开更多
We focus on a modified version of the non-Hermitian Aubry-Andre-Harper(AAH)model,which has garnered significant attention due to its ability to investigate localization phenomena,metal-insulator transitions,and topolo...We focus on a modified version of the non-Hermitian Aubry-Andre-Harper(AAH)model,which has garnered significant attention due to its ability to investigate localization phenomena,metal-insulator transitions,and topological phase transitions.We have made two key modifications to the non-Hermitian AAH model:First,we introduce a mosaic structure that allows for the mixing of localized and extended states,resulting in the appearance of mobility edges,which is a feature that is not present in the original non-Hermitian AAH model.In the insulating phase,leveraging Fields Medal winner Avila's global theory,our work derives a theoretical description of the localization length,a crucial parameter previously unavailable in the non-Hermitian AAH model,and obtains the exact expression for mobility edges.We studied the variation of the energy spectrum with the amplitude and quantitatively determined the topological phase transition point within the spectrum.Furthermore,we introduced an asymmetric parameter g and calculated its corresponding localization length,the location of mobility edges,as well as the precise expressions for its extended and localized states.By quantitatively calculating the Lyapunov exponent of dual models,our work reveals an interesting fact about the robustness of localized states:within an appropriate relationship between g and the coupling potential strength,the localized states exhibit similar characteristics to those in the mosaic non-Hermitian AAH model.Our work offers a more complete and nuanced understanding of localization phenomena in disordered non-Hermitian systems,paving the way for further research in this promising field.展开更多
Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered ...Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.展开更多
文摘We present a method of 3D image mosaicing for real 3D representation of roadside buildings, and implement a Web-based interactive visualization environment for the 3D video mosaics created by 3D image mosaicing. The 3D image mo- saicing technique developed in our previous work is a very powerful method for creating textured 3D-GIS data without excessive data processing like the laser or stereo system. For the Web-based open access to the 3D video mosaics, we build an interactive visualization environment using X3D, the emerging standard of Web 3D. We conduct the data preprocessing for 3D video mosaics and the X3D modeling for textured 3D data. The data preprocessing includes the conversion of each frame of 3D video mosaics into concatenated image files that can be hyperlinked on the Web. The X3D modeling handles the representation of concatenated images using necessary X3D nodes. By employing X3D as the data format for 3D image mosaics, the real 3D representation of roadside buildings is extended to the Web and mobile service systems.
基金supported by National Natural Science Foundation of China projects (81770938 to J.Z. and 81570822 to K.Y.)the Zhejiang Provincial Natural Science Foundation project (LZ15H120001 to J.Z.)the Fundamental Research Funds for the Central Universities (2016FZA7010 and 2017FZA7001 to J.Z.)。
文摘Although the unique organization of vertebrate cone mosaics was first described long ago,both their underlying molecular basis and physiological significance are largely unknown.Here,we demonstrate that Crumbs proteins,the key regulators of epithelial apical polarity,establish the planar cellular polarity of photoreceptors in zebrafish.Via heterophilic Crb2a-Crb2b interactions,the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors.The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors,thereby stabilizing the geometric organization of cone mosaics.Notably,loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation.These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness.
基金the National Major Basic Researched Programe of China(2004CB418305)the National Natural Foundation of China(40375008)
文摘This paper investigated several approaches to remapping and combining multiple-radar reflectivity fields onto a unified 3D Cartesian grid with high spatial and temporal resolutions, and analyzed systematic observation differences among multiple radars. The remapping approaches were evaluated by inspecting the spatial consistency of the reflectivity fields on vertical and horizontal cross sections on the equidistant line of radars, and the intensity change of 1-h accumulated precipitation before and after interpolation. The combining approaches were evaluated by continuity examination. The results show that for remapping schemes, the vertical interpolation with nearest neighbor on the range-azimuth plane is the most reasonable scheme that provides consecutive reflectivity fields and retains the high-resolution structure comparable to that of the raw data; for multiple-radar data mosaics, the distance-exponential-weighted mean scheme provides spatially consistent reflectivity mosaics. The mosaics can mitigate various problems caused by the radar beam geometry such as the cone of silence.
基金supported by the National Natural Science Foundation of China(Grant Nos.32402564,32330096,32372631)Hebei Natural Science Foundation(Grant No.C2024204246)+1 种基金the Pinduoduo-China Agricultural University Research Fund(Grant No.PC2023B02018)the 2115 Talent Development Program of China Agricultural University.
文摘Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic virus(TuMV)is a major pathogen affecting Chinese cabbages,leading to severe yield losses(Li et al.,2019).Traditional control measures have shown limited efficacy,and the long-term use of chemical pesticides has led to significant issues such as environmental pollution and pathogen resistance(Samara et al.,2021;Lu et al.,2022).Biologicallyderived pesticides have garnered considerable attention owing to their eco-friendly attributes(Ayilara et al.,2023).γ-Aminobutyric acid(GABA),initially discovered in potato tubers,has been proven to regulate immune responses and enhance resistance to fungal and bacterial pathogens by modulating reactive oxygen species and stress-related hormone signals(Tarkowski et al.,2020;Wang et al.,2025).But biologically-derived agents typically face challenges such as large particle size and instability,which limit their practical application and bioavailability(Daraban et al.,2023).
文摘Assisted hatching(AH)is commonly performed before trophectoderm(TE)biopsy for preimplantation genetic testing for aneuploidy(PGT-A),yet whether AH timing influences the detection of chromosomal mosaicism remains uncertain.Beyond timing,procedural choices in PGT-A must balance diagnostic yield against potential harm from additional handling(e.g.,repeat TE biopsy or cryopreservation steps),as underscored by a recent systematic review and meta-analysis[1].
文摘China and Nepal deepen cooperation to promote traditional medicine across the Himalayan region.THE Himalayan region features a rich mosaic of cultures that are distinct yet deeply intertwined.Traditional medicine,a common treasure of human civilization,has deep roots in this region.
文摘Jute is the golden fiber crop of Bangladesh. Viral and Nemic diseases are the major threats to fiber crops, and the fiber yield decreases drastically as the disease infection increases. An experiment was conducted to screen and evaluate ten Jute and allied fiber varieties i.e. BJRI Kenaf-4, BJRI Kenaf-3, HS-95 (Kenaf), HS-24 (Mesta pat), HC-2 (Kenaf), BJRI Deshi pat-8, BJRI Deshi pat-7, BJRI Deshi pat-5, BJRI Deshi pat-6 and CVL-1 against mosaic virus and root-knot nematode disease. It was done in RCBD design with three replications at Sher-e-Bangla Agricultural University (SAU) central farm, Dhaka-1207, from June 2018 to March 2019. In the case of viral infection, variety 4 (HS-24 (Mesta pat)) showed maximum virus incidence (23.11%), whereas variety 2 (BJRI Kenaf-3) had no virus evidence. The most susceptible nematode-infected variety was found in varieties 5 (HC-2 (Kenaf)) and 9 (BJRI Deshi pat-6), which showed 80% incidence, whereas variety 2 (BJRI Kenaf-3) was highly resistance. The growth and yield results showed a significant (0.05%) reduction of plant height (cm) and fiber weight (t/ha) with an increase in the percentage of virus and nematode incidence against all varieties. The highest fiber weight (1.47 t/ha) and the lowest viral (0%) and nemic infection (0%) were found in variety 2 (BJRI Kenaf-3), which showed better performance compared to other varieties. These results revealed that variety 2 (BJRI Kenaf-3) had the best variety in the present study.
基金supported by the National Key Research and Development Program of China(2022YFF1001502)Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)Major Projects of Technological Innovation 2030(2023ZD04037).
文摘Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.
基金supported by the National Key Research and Development Program of China(Grant no.2021YFC2803304)the National Natural Science Foundation of China(Grant nos.52192691 and 52192690)the Program of Shanghai Academic/Technology Research Leader(Grant no.22XD1403600).
文摘Using nine ice-tethered buoys deployed across the marginal ice zone(MIZ)and pack ice zone(PIZ)north of the Laptev Sea during the expedition of the Multidisciplinary drifting Observatory for the Study of Arctic Climate(MOSAiC)in 2019-2020,we characterized the spatiotemporal variations in sea ice kinematics and deformation between October 2019 and July 2020 in the Transpolar Drift(TPD).From October to November,the buoys were in the upstream area of the TPD;spatial variations of deformation rates were significantly correlated with initial ice thickness(R=−0.84,P<0.05).From December 2019 to March 2020,the buoys were in the high Arctic and the ice cover was consolidated;heterogeneity in ice kinematics as measured across the buoys reduced by 65%.From April to May 2020,the buoys were in the downstream TPD;amplified spatial variations in ice kinematics were observed.This is because two buoys had drifted over the shallow waters north of Svalbard earlier;trajectory-stretching exponents derived from the data from these two buoys indicate deformation rates(10.6 d^(−1))that were about twice those in the deep basin(4.2 d^(−1)).By June 2020,a less consolidated ice pack and enhanced tidal forcing in the Fram Strait MIZ resulted in ice deformation with a semi-diurnal power spectral density of>0.25 d^(−1),which is about 1.5 times that in PIZ.Therefore,in both the upstream and downstream regions of the TPD,the transition between the MIZ and the PIZ contributes to the spatial and seasonal variations of sea ice motion and deformation.The results from this study can be used to support the characterization of the momentum balance and influencing factors during the ice advection along the TPD,which is a crucial corridor for Arctic sea ice outflow to the north Atlantic Ocean.
基金Project supported by the National Natural Science Foundation of China(Grant No.12301218)。
文摘We focus on a modified version of the non-Hermitian Aubry-Andre-Harper(AAH)model,which has garnered significant attention due to its ability to investigate localization phenomena,metal-insulator transitions,and topological phase transitions.We have made two key modifications to the non-Hermitian AAH model:First,we introduce a mosaic structure that allows for the mixing of localized and extended states,resulting in the appearance of mobility edges,which is a feature that is not present in the original non-Hermitian AAH model.In the insulating phase,leveraging Fields Medal winner Avila's global theory,our work derives a theoretical description of the localization length,a crucial parameter previously unavailable in the non-Hermitian AAH model,and obtains the exact expression for mobility edges.We studied the variation of the energy spectrum with the amplitude and quantitatively determined the topological phase transition point within the spectrum.Furthermore,we introduced an asymmetric parameter g and calculated its corresponding localization length,the location of mobility edges,as well as the precise expressions for its extended and localized states.By quantitatively calculating the Lyapunov exponent of dual models,our work reveals an interesting fact about the robustness of localized states:within an appropriate relationship between g and the coupling potential strength,the localized states exhibit similar characteristics to those in the mosaic non-Hermitian AAH model.Our work offers a more complete and nuanced understanding of localization phenomena in disordered non-Hermitian systems,paving the way for further research in this promising field.
文摘Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.
