We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the m...We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaecharide derivatives, obtained with 1-Phenyl-3-methyl-5- pyrazolone （PMP）, were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1-2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration （r^2〉0.998）. The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.展开更多
A new compound named 1-hydrate, 3-deoxy-α-D-tagatofuranose was isolated from Swertia punicea Hemsl. The structure of compound was determined by 1D and 2D NMR, HRESIMS techniques.
Fourier Transform Infrared Spectroscopy (FTIR) was used to quantify total monosaccharide content in the bacterium Enterobacter cloacae and several of its biofilm mutants. Bacterial biofilm samples were grown on trypti...Fourier Transform Infrared Spectroscopy (FTIR) was used to quantify total monosaccharide content in the bacterium Enterobacter cloacae and several of its biofilm mutants. Bacterial biofilm samples were grown on trypticase soy agar, and 30 μL aliquots of aqueous sample bacterial plus biofilm were deposited into the center of barium fluoride crystals and dried at 50°C for 1-hour before being scanned by FTIR. The total amounts of monosaccharides were estimated using the absorbance of the mono-saccharide peak, 1192 - 958 cm–1, and normalized using the amide II peak, 1585 - 1483 cm–1. This method provided a linear correlation between the absorbance of the monosaccharide peak and concentration of monosaccharide in standard monosaccharides, fructose, glucose, mannose, and rhamnose, over a concentration range of 0.5 - 2.0 mg/mL.展开更多
Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industria...Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industrial applications, such as furan production of furfural and hydroxymethyl furfural. Hydrothermal treatment at 200°C and ultrasonic irradiation pretreatment were used to recover monosaccharides from the abovementioned resources. The monosaccharide recovery from peat was shown to be higher than that from peatified wood. The conversion to organic acids is considered to proceed rapidly because acids are always detected with monosaccharides. This conversion is outstripped by the organic acid-to-gas reaction for treatment times longer than 20 min. The monosaccharide recovery from peatified wood was improved by the ultrasonication pretreatment. It is considered that ultrasonic irradiation broke down lignin blockages, enabling water molecules to access the carbohydrates more easily in the subsequent hydrothermal treatment.展开更多
The interaction between monosaccharides exhibits an important role in the assembly of monosaccharide-containing molecules. In this work, three common monosaccharides, glucose, galactose and mannose, are employed to in...The interaction between monosaccharides exhibits an important role in the assembly of monosaccharide-containing molecules. In this work, three common monosaccharides, glucose, galactose and mannose, are employed to investigate the effect of monosaccharide on the self-assembly of benzenetricarboxamide(BTA) core-containing molecules. In the presence of monosaccharides, three benzenetricarboxamide derivatives aggregate into different ordered structures. When alanine linkers are introduced to these molecules between the core and the monosacchride, morphologies of three types of monosaccharide BTAs turned to disordered, meanwhile their structures become similar with the increase of the length of alanine linkers, indicating the disappearance of the monosaccharide effects.展开更多
Poly（phenylacetylene）s beating monosaccharide pendant groups are synthesized in high yields by [Rh（nbd）Cl]2 catalyst. The polymers have high molecular weights and give satisfactory spectroscopic data corresponding...Poly（phenylacetylene）s beating monosaccharide pendant groups are synthesized in high yields by [Rh（nbd）Cl]2 catalyst. The polymers have high molecular weights and give satisfactory spectroscopic data corresponding to their molecular structures. They are thermally quite stable （≥ 300℃） and show strong circular dichroism signals in the visible spectral region owing to the helicity of the polyene backbone. The monosaccharide-containing polyacetylenes are cytophilic and can stimulate the growth of living cells.展开更多
A new lipophilic monosaccharide, erigearide A （1）, was isolated from the aerial parts of Erigeron annuus （Lima.） Pers. Its structure was elucidated by analysis of spectroscopic evidence.
Irritable bowel syndrome (IBS) is a lifelong condition with a high prevalence among children and adults. As the diet is a frequent factor that triggers the symptoms, it has been assumed that by avoiding the consumptio...Irritable bowel syndrome (IBS) is a lifelong condition with a high prevalence among children and adults. As the diet is a frequent factor that triggers the symptoms, it has been assumed that by avoiding the consumption of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAP), the symptoms might be improved. Therefore, in the past decade, low FODMAP diet has been intensively investigated in the management of IBS. The capacity of FODMAPs to trigger the symptoms in patients with IBS was related to the stimulation of mechanoreceptors in the small and large intestine. This stimulation appears as a response to a combination of increased luminal water (the osmotic effect) and the release of gases (carbon dioxide and hydrogen) due to the fermentation of oligosaccharides and malabsorption of fructose, lactose and polyols. Numerous studies have been published regarding the efficacy of a low FODMAP diet compared to a traditional diet in releasing the IBS symptoms in adults, but there are only a few studies in the juvenile population. The aim of this review is to analyze the current data on both low FODMAP diet in children with IBS and the effects on their nutritional status and physiological development, given the fact that it is a restrictive diet.展开更多
Oxyphosphorane reacted with glucose to give disaccharide which was confirmed by FAB-MS and C-13-NMR. The mechanism for formation of oligosaccharides through the participation of phosphorus was proposed.
To the Editor:Lung cancer remains the leading cause of cancer-related deaths worldwide.[1]Its histological classification includes non-small cell lung cancer(NSCLC),which encompasses adenocarcinoma,squamous cell carci...To the Editor:Lung cancer remains the leading cause of cancer-related deaths worldwide.[1]Its histological classification includes non-small cell lung cancer(NSCLC),which encompasses adenocarcinoma,squamous cell carcinoma,and large cell carcinoma.Among these,lung adenocarcinoma(LUAD)is the most common subtype.Visceral pleural invasion(VPI)is a significant factor influencing tumor grade and serves as an independent risk factor for the prognosis of patients with lung cancer.However,due to the lack of measurable markers in most cases,it can only be detected through imaging techniques and pathological examinations.展开更多
A significant reaction in the synthesis of biomass-based chemicals is the catalyst-based and targeted oxidation of monosaccharides into valuable sugar acids.In this study,an activated carbon supported gold catalyst wa...A significant reaction in the synthesis of biomass-based chemicals is the catalyst-based and targeted oxidation of monosaccharides into valuable sugar acids.In this study,an activated carbon supported gold catalyst was used to oxidize glucose and xylose to gluconic acid and xylonic acid under neutral condition.Optimization of reaction conditions for the catalysts was performed using both a batch reactor and a flow-through reactor.In a batch reactor,the yields of gluconic and xylonic acid reached 93%and 92%,respectively,at 90℃ within 180 min.In a flow reactor,both reactions reached a similar yield at 80℃ with the weight hourly space velocity of 47.1 h^(-1).The reaction kinetics were explored in the flow reactor.The oxidation of glucose and xylose to gluconic and xylonic acid followed a first-order kinetics and the turnover frequency was 0.195 and 0.161 s^(-1),respectively.The activation energy was evaluated to be 60.58 and 59.30 kJ·mol^(-1),respectively.This study presents an environmentally friendly and feasible method for the selective oxidation of monosaccharides using an activated carbon supported gold catalyst,benefiting the high-value application of carbohydrates.展开更多
The efficient fractionation and recovery of monosaccharides(xylose and glucose)from lignocellulosic biomass facilitates subsequent sugar-based derivative production.This study introduces a one-potγ-valerolactone/CuCl...The efficient fractionation and recovery of monosaccharides(xylose and glucose)from lignocellulosic biomass facilitates subsequent sugar-based derivative production.This study introduces a one-potγ-valerolactone/CuCl_(2)biphasic pretreatment system(100-mmol·L^(-1)CuCl_(2),180°C,60 min)capable of achieving removal rates of 92.25%and 90.64%for xylan and lignin,respectively,while retaining 83.88%of cellulose.Compared to other metal chlorides(NaCl,LiCl,FeCl_(3),and AlCl_(3)),theγ-valerolactone/CuCl_(2)system recovered 121.2 mg·(g eucalyptus)^(-1)of xylose and 55.96 mg·(g eucalyptus)^(-1)of glucose during the pretreatment stage and 339.2 mg·(g eucalyptus)^(-1)of glucose during the enzymatic hydrolysis stage(90.78%of glucose yield),achieving a total monosaccharide recovery of 86.31%.In addition,the recovery ofγ-valerolactone was 79.33%,exhibiting minimal changes relative to the pretreatment performance.The method proposed in this study allows a high total monosaccharides recovery and a circular economy-oriented pretreatment approach,offering a viable pathway for biorefinery.展开更多
The early responsive to dehydration-like(ERDL or ERD)subfamily,subclade within the monosaccharide transporter(MST)superfamily,is crucial in the regulation of growth and seed yield in Arabidopsis.Here,we identified Os ...The early responsive to dehydration-like(ERDL or ERD)subfamily,subclade within the monosaccharide transporter(MST)superfamily,is crucial in the regulation of growth and seed yield in Arabidopsis.Here,we identified Os ERD5 as an At ERDL6 homologue and explored the function of Os ERD5.We found that Os ERD5 overexpression significantly enhanced the tiller number and grain yield of rice.Os ERD5 was widely expressed in aboveground tissues,encoded a tonoplast-localized protein,and exhibited transport activities for fructose,glucose and mannose when expressed in yeast.Expression character assay revealed that Os ERD5 mediated hexose efflux across tonoplasts and participated in maintaining the diurnal rhythm-regulated intracellular hexose homeostasis.Additional physiological and molecular evidence showed that Os ERD5 overexpression promoted vacuolar glucose efflux,enhanced sucrose synthesis and transport,increased sugar content in the shoot base,and promoted rice tillering by activating the synthesis of cytokinin simultaneously repressing strigolactone and gibberellin signaling.This study elucidates the function of Os ERD5 and the mechanism underlying the overexpression of Os ERD5 increasing rice tillering and yield.展开更多
The study investigated the effects of ethanol precipitation on the polysaccharide components obtained from 3 different oak acorns.The variables factors were ethanol precipitation times(1×or 2×),tree species(...The study investigated the effects of ethanol precipitation on the polysaccharide components obtained from 3 different oak acorns.The variables factors were ethanol precipitation times(1×or 2×),tree species(Quercus variabilis,Quercus aliena,Quercus dentata)and raw materials(whole acorn fruit or kernels).Through detections of acorn polysaccharide concentrations from different species and components analyses,we found that the second ethanol precipitation had a better extraction effect,and acorn polysaccharides were proved to be mainly composed of glucose,galacturonic acid,arabinose and galactose.Compared with other groups,the highest content of polysaccharides was proved to be extracted from Q.variabilis kernels,thus,acorn kernels should be suggested as the proper material for the extraction of acorn polysaccharides.Interestingly,acorn polysaccharides functionally alleviated liver and kidney damages in type 2 diabetes mice,they also had noticeable hypoglycemic effects and regulated insulin secretion,particularly in combination with metformin and D-mannuronic acid.展开更多
Vascular plants contain two gene families that encode monosaccharide transporter proteins. The classical monosaccharide transporter（-like） gene superfamily is large and functionally diverse, while the recently ident...Vascular plants contain two gene families that encode monosaccharide transporter proteins. The classical monosaccharide transporter（-like） gene superfamily is large and functionally diverse, while the recently identified SWEET transporter family is smaller and, thus far, only found to transport glucose. These transporters play essential roles at many levels, ranging from organelles to the whole plant. Many family members are essential for cellular homeostasis and reproductive success. Although most transporters do not directly participate in long-distance transport, their indirect roles greatly impact carbon allocation and transport flux to the heterotrophic tissues of the plant. Functional characterization of some members from both gene families has revealed their diverse roles in carbohydrate partitioning, phloem function, resource allocation, plant defense, and sugar signaling. This review highlights the broad impacts and implications of monosaccharide transport by describing some of the functional roles of the monosaccharide transporter（-like） superfamily and the SWEET transporter family.展开更多
Dilution enthalpies of sodium chloride and some monosaccharides (glucose, galactose, xylose, arabinose, and fructose) in water and mixing enthalpies of aqueous sodium chloride and these monosaccharide solutions were m...Dilution enthalpies of sodium chloride and some monosaccharides (glucose, galactose, xylose, arabinose, and fructose) in water and mixing enthalpies of aqueous sodium chloride and these monosaccharide solutions were measured by using an improved precision semimicro-titration calorimeter. Transfer enthalpies of sodium chloride from water to aqueous saccharide solutions were evaluated as well as enthalpy interaction parameters of sodium chloride with these monosaccharides in water. Combined with Gibbs energy interaction parameters, entropy interaction parameters were also obtained. The results show that interactions of the saccharides with sodium chloride depend on the stereochemistry of saccharide molecules. These interaction parameters can identify stereochemical structure of saccharide molecules. Keywords sodium chloride - monosaccharide - enthalpy interaction parameter - entropy interaction parameter - calorimetry展开更多
Dear Editor,The human gastrointestinal tract is colonized by trillions of microorganisms,the majority of which cannot be cultured (Wu et al.,2018).Numerous glycans are expressed on the surface of gut microbiota that a...Dear Editor,The human gastrointestinal tract is colonized by trillions of microorganisms,the majority of which cannot be cultured (Wu et al.,2018).Numerous glycans are expressed on the surface of gut microbiota that are characteristic of different strains,and the diversity of these glycans is partially attributed to variations in their monosaccharides.展开更多
Monosaccharides are one of the most important structural components of biomolecules, such as polysac- charides, nucleic acids, glycolipids and glycoproteins. In structural analysis of polysaccharides and gly- coconjug...Monosaccharides are one of the most important structural components of biomolecules, such as polysac- charides, nucleic acids, glycolipids and glycoproteins. In structural analysis of polysaccharides and gly- coconjugates, the absolute configurations （D or L） of the constituent monosaccharides are usually deter- mined by measurement of the optical rotation, CD spectra or characteristic chromatographic retention behavior. However, each method has its unique advantages and limitations which should be considered while using them. In this review, an overview of the different methods for the determination of absolute configuration of monosaccharides and their underlying principles are summarized to serve as a reference for researchers.展开更多
A simple,sensitive and direct method was developed for compositional analysis of plant polysaccharides using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass s...A simple,sensitive and direct method was developed for compositional analysis of plant polysaccharides using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry(HILIC-UPLC-TQ-MS/MS)in multiple-reaction monitoring(MRM).The effects of chromatographic columns,scan time,additive types,展开更多
The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of t...The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of the derivatives provide information on their linkage Positions and iso- merism of the individual monoscaccharide units.The results indicated that among the derivatives of the oligosaccharides analyzed,those with 1—4 and 1—6 linkages all presented the ion peaks at m/z 287,sometimes one more peak at m/z 449.Furthermore,a relationship was found between the linkage positions and the intensity orders of the derivative ions.Finally,the derivatives of the disaccharides with a galactose presented an intense ion peak at m/z 347,and those of oligosaccharides with 1—6 linkage to a galactose at terminal presented the ion at m/z 317.In the case of oligosaccharides with a fructose residue,characteristic ion of m/z 155 was produced.The conditions of stereoselective derivatizations and mass spectrometry were studied,in order to obtain a better reproducibility of the mass spectra.展开更多
基金Supported by the Key Technology Research & Development Program of Shandong Province (No 2007GG1005007)
文摘We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaecharide derivatives, obtained with 1-Phenyl-3-methyl-5- pyrazolone （PMP）, were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1-2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration （r^2〉0.998）. The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.
基金supported by the National Natural Science Foundation of China(No.30271590)
文摘A new compound named 1-hydrate, 3-deoxy-α-D-tagatofuranose was isolated from Swertia punicea Hemsl. The structure of compound was determined by 1D and 2D NMR, HRESIMS techniques.
文摘Fourier Transform Infrared Spectroscopy (FTIR) was used to quantify total monosaccharide content in the bacterium Enterobacter cloacae and several of its biofilm mutants. Bacterial biofilm samples were grown on trypticase soy agar, and 30 μL aliquots of aqueous sample bacterial plus biofilm were deposited into the center of barium fluoride crystals and dried at 50°C for 1-hour before being scanned by FTIR. The total amounts of monosaccharides were estimated using the absorbance of the mono-saccharide peak, 1192 - 958 cm–1, and normalized using the amide II peak, 1585 - 1483 cm–1. This method provided a linear correlation between the absorbance of the monosaccharide peak and concentration of monosaccharide in standard monosaccharides, fructose, glucose, mannose, and rhamnose, over a concentration range of 0.5 - 2.0 mg/mL.
文摘Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industrial applications, such as furan production of furfural and hydroxymethyl furfural. Hydrothermal treatment at 200°C and ultrasonic irradiation pretreatment were used to recover monosaccharides from the abovementioned resources. The monosaccharide recovery from peat was shown to be higher than that from peatified wood. The conversion to organic acids is considered to proceed rapidly because acids are always detected with monosaccharides. This conversion is outstripped by the organic acid-to-gas reaction for treatment times longer than 20 min. The monosaccharide recovery from peatified wood was improved by the ultrasonication pretreatment. It is considered that ultrasonic irradiation broke down lignin blockages, enabling water molecules to access the carbohydrates more easily in the subsequent hydrothermal treatment.
基金The Ministry of Science and Technology of China and the National Natural Science Foundation of China(Nos.91527305 and 51322306)are acknowledged for their financial support
文摘The interaction between monosaccharides exhibits an important role in the assembly of monosaccharide-containing molecules. In this work, three common monosaccharides, glucose, galactose and mannose, are employed to investigate the effect of monosaccharide on the self-assembly of benzenetricarboxamide(BTA) core-containing molecules. In the presence of monosaccharides, three benzenetricarboxamide derivatives aggregate into different ordered structures. When alanine linkers are introduced to these molecules between the core and the monosacchride, morphologies of three types of monosaccharide BTAs turned to disordered, meanwhile their structures become similar with the increase of the length of alanine linkers, indicating the disappearance of the monosaccharide effects.
基金This work was partially supported by the Research Grants Council of Hong Kong(Nos. 602706, HKU2/05C, 603505,603304, and 664903).
文摘Poly（phenylacetylene）s beating monosaccharide pendant groups are synthesized in high yields by [Rh（nbd）Cl]2 catalyst. The polymers have high molecular weights and give satisfactory spectroscopic data corresponding to their molecular structures. They are thermally quite stable （≥ 300℃） and show strong circular dichroism signals in the visible spectral region owing to the helicity of the polyene backbone. The monosaccharide-containing polyacetylenes are cytophilic and can stimulate the growth of living cells.
基金the National Natural Science Foundation of China(No.30772637)the Western Doctoral Foundation of Chinese Academy of Sciences(presided by J.X.Pu).
文摘A new lipophilic monosaccharide, erigearide A （1）, was isolated from the aerial parts of Erigeron annuus （Lima.） Pers. Its structure was elucidated by analysis of spectroscopic evidence.
文摘Irritable bowel syndrome (IBS) is a lifelong condition with a high prevalence among children and adults. As the diet is a frequent factor that triggers the symptoms, it has been assumed that by avoiding the consumption of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAP), the symptoms might be improved. Therefore, in the past decade, low FODMAP diet has been intensively investigated in the management of IBS. The capacity of FODMAPs to trigger the symptoms in patients with IBS was related to the stimulation of mechanoreceptors in the small and large intestine. This stimulation appears as a response to a combination of increased luminal water (the osmotic effect) and the release of gases (carbon dioxide and hydrogen) due to the fermentation of oligosaccharides and malabsorption of fructose, lactose and polyols. Numerous studies have been published regarding the efficacy of a low FODMAP diet compared to a traditional diet in releasing the IBS symptoms in adults, but there are only a few studies in the juvenile population. The aim of this review is to analyze the current data on both low FODMAP diet in children with IBS and the effects on their nutritional status and physiological development, given the fact that it is a restrictive diet.
文摘Oxyphosphorane reacted with glucose to give disaccharide which was confirmed by FAB-MS and C-13-NMR. The mechanism for formation of oligosaccharides through the participation of phosphorus was proposed.
基金Natural Science Foundation of China(Nos.30981672585 and 11804151)Natural Science Foundation of Shandong Province of China(Nos.ZR2020QH286 and ZR2020QC059)
文摘To the Editor:Lung cancer remains the leading cause of cancer-related deaths worldwide.[1]Its histological classification includes non-small cell lung cancer(NSCLC),which encompasses adenocarcinoma,squamous cell carcinoma,and large cell carcinoma.Among these,lung adenocarcinoma(LUAD)is the most common subtype.Visceral pleural invasion(VPI)is a significant factor influencing tumor grade and serves as an independent risk factor for the prognosis of patients with lung cancer.However,due to the lack of measurable markers in most cases,it can only be detected through imaging techniques and pathological examinations.
基金support from the National Key Research and Development Program of China(Grant No.2021YFC2101604)the National Natural Science Foundation of China(Grant No.22108088)the Natural Science Foundation of Guangdong,China(Grant No.2023A1515012740)。
文摘A significant reaction in the synthesis of biomass-based chemicals is the catalyst-based and targeted oxidation of monosaccharides into valuable sugar acids.In this study,an activated carbon supported gold catalyst was used to oxidize glucose and xylose to gluconic acid and xylonic acid under neutral condition.Optimization of reaction conditions for the catalysts was performed using both a batch reactor and a flow-through reactor.In a batch reactor,the yields of gluconic and xylonic acid reached 93%and 92%,respectively,at 90℃ within 180 min.In a flow reactor,both reactions reached a similar yield at 80℃ with the weight hourly space velocity of 47.1 h^(-1).The reaction kinetics were explored in the flow reactor.The oxidation of glucose and xylose to gluconic and xylonic acid followed a first-order kinetics and the turnover frequency was 0.195 and 0.161 s^(-1),respectively.The activation energy was evaluated to be 60.58 and 59.30 kJ·mol^(-1),respectively.This study presents an environmentally friendly and feasible method for the selective oxidation of monosaccharides using an activated carbon supported gold catalyst,benefiting the high-value application of carbohydrates.
基金Opening Project of Guangxi Key Laboratory of Clean Pulp&Papermaking and Pollution Control(Grant No.2021KF41)Young Innovative Talents Scientific Research Project of Guangxi(Grant No.AD23026256)Guangxi Natural Science Foundation of China(Grant No.2023GXNSFGA026001).
文摘The efficient fractionation and recovery of monosaccharides(xylose and glucose)from lignocellulosic biomass facilitates subsequent sugar-based derivative production.This study introduces a one-potγ-valerolactone/CuCl_(2)biphasic pretreatment system(100-mmol·L^(-1)CuCl_(2),180°C,60 min)capable of achieving removal rates of 92.25%and 90.64%for xylan and lignin,respectively,while retaining 83.88%of cellulose.Compared to other metal chlorides(NaCl,LiCl,FeCl_(3),and AlCl_(3)),theγ-valerolactone/CuCl_(2)system recovered 121.2 mg·(g eucalyptus)^(-1)of xylose and 55.96 mg·(g eucalyptus)^(-1)of glucose during the pretreatment stage and 339.2 mg·(g eucalyptus)^(-1)of glucose during the enzymatic hydrolysis stage(90.78%of glucose yield),achieving a total monosaccharide recovery of 86.31%.In addition,the recovery ofγ-valerolactone was 79.33%,exhibiting minimal changes relative to the pretreatment performance.The method proposed in this study allows a high total monosaccharides recovery and a circular economy-oriented pretreatment approach,offering a viable pathway for biorefinery.
基金supported by the National Natural Science Foundation of China(32401743)the Earmarked Fund for the China Agriculture Research System(CARS-01)+1 种基金the Hunan Natural Science Foundation Project(2021JJ40235)the Science and Technology Innovation Program of Hunan Province(2022RC3053)。
文摘The early responsive to dehydration-like(ERDL or ERD)subfamily,subclade within the monosaccharide transporter(MST)superfamily,is crucial in the regulation of growth and seed yield in Arabidopsis.Here,we identified Os ERD5 as an At ERDL6 homologue and explored the function of Os ERD5.We found that Os ERD5 overexpression significantly enhanced the tiller number and grain yield of rice.Os ERD5 was widely expressed in aboveground tissues,encoded a tonoplast-localized protein,and exhibited transport activities for fructose,glucose and mannose when expressed in yeast.Expression character assay revealed that Os ERD5 mediated hexose efflux across tonoplasts and participated in maintaining the diurnal rhythm-regulated intracellular hexose homeostasis.Additional physiological and molecular evidence showed that Os ERD5 overexpression promoted vacuolar glucose efflux,enhanced sucrose synthesis and transport,increased sugar content in the shoot base,and promoted rice tillering by activating the synthesis of cytokinin simultaneously repressing strigolactone and gibberellin signaling.This study elucidates the function of Os ERD5 and the mechanism underlying the overexpression of Os ERD5 increasing rice tillering and yield.
基金supported in part by“The Fundamental Research Funds for the Central Universities”(BLX202218).
文摘The study investigated the effects of ethanol precipitation on the polysaccharide components obtained from 3 different oak acorns.The variables factors were ethanol precipitation times(1×or 2×),tree species(Quercus variabilis,Quercus aliena,Quercus dentata)and raw materials(whole acorn fruit or kernels).Through detections of acorn polysaccharide concentrations from different species and components analyses,we found that the second ethanol precipitation had a better extraction effect,and acorn polysaccharides were proved to be mainly composed of glucose,galacturonic acid,arabinose and galactose.Compared with other groups,the highest content of polysaccharides was proved to be extracted from Q.variabilis kernels,thus,acorn kernels should be suggested as the proper material for the extraction of acorn polysaccharides.Interestingly,acorn polysaccharides functionally alleviated liver and kidney damages in type 2 diabetes mice,they also had noticeable hypoglycemic effects and regulated insulin secretion,particularly in combination with metformin and D-mannuronic acid.
文摘Vascular plants contain two gene families that encode monosaccharide transporter proteins. The classical monosaccharide transporter（-like） gene superfamily is large and functionally diverse, while the recently identified SWEET transporter family is smaller and, thus far, only found to transport glucose. These transporters play essential roles at many levels, ranging from organelles to the whole plant. Many family members are essential for cellular homeostasis and reproductive success. Although most transporters do not directly participate in long-distance transport, their indirect roles greatly impact carbon allocation and transport flux to the heterotrophic tissues of the plant. Functional characterization of some members from both gene families has revealed their diverse roles in carbohydrate partitioning, phloem function, resource allocation, plant defense, and sugar signaling. This review highlights the broad impacts and implications of monosaccharide transport by describing some of the functional roles of the monosaccharide transporter（-like） superfamily and the SWEET transporter family.
基金This work was supported by the Outstanding Youth Science Foundation of Henan Province(Grant No.0112000500)the Innovator Foundation for Colleges and Universities of Henan Province.
文摘Dilution enthalpies of sodium chloride and some monosaccharides (glucose, galactose, xylose, arabinose, and fructose) in water and mixing enthalpies of aqueous sodium chloride and these monosaccharide solutions were measured by using an improved precision semimicro-titration calorimeter. Transfer enthalpies of sodium chloride from water to aqueous saccharide solutions were evaluated as well as enthalpy interaction parameters of sodium chloride with these monosaccharides in water. Combined with Gibbs energy interaction parameters, entropy interaction parameters were also obtained. The results show that interactions of the saccharides with sodium chloride depend on the stereochemistry of saccharide molecules. These interaction parameters can identify stereochemical structure of saccharide molecules. Keywords sodium chloride - monosaccharide - enthalpy interaction parameter - entropy interaction parameter - calorimetry
基金supported by the State Key Laboratory of Microbial Technology Projects Fundthe National Natural Science Foundation of China(31770997,31500648)
文摘Dear Editor,The human gastrointestinal tract is colonized by trillions of microorganisms,the majority of which cannot be cultured (Wu et al.,2018).Numerous glycans are expressed on the surface of gut microbiota that are characteristic of different strains,and the diversity of these glycans is partially attributed to variations in their monosaccharides.
基金supported by State Key Program of National Natural Science Foundation of China (grant number 81430095)
文摘Monosaccharides are one of the most important structural components of biomolecules, such as polysac- charides, nucleic acids, glycolipids and glycoproteins. In structural analysis of polysaccharides and gly- coconjugates, the absolute configurations （D or L） of the constituent monosaccharides are usually deter- mined by measurement of the optical rotation, CD spectra or characteristic chromatographic retention behavior. However, each method has its unique advantages and limitations which should be considered while using them. In this review, an overview of the different methods for the determination of absolute configuration of monosaccharides and their underlying principles are summarized to serve as a reference for researchers.
文摘A simple,sensitive and direct method was developed for compositional analysis of plant polysaccharides using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry(HILIC-UPLC-TQ-MS/MS)in multiple-reaction monitoring(MRM).The effects of chromatographic columns,scan time,additive types,
基金Project supported by the National Natural Science Foundation of China
文摘The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of the derivatives provide information on their linkage Positions and iso- merism of the individual monoscaccharide units.The results indicated that among the derivatives of the oligosaccharides analyzed,those with 1—4 and 1—6 linkages all presented the ion peaks at m/z 287,sometimes one more peak at m/z 449.Furthermore,a relationship was found between the linkage positions and the intensity orders of the derivative ions.Finally,the derivatives of the disaccharides with a galactose presented an intense ion peak at m/z 347,and those of oligosaccharides with 1—6 linkage to a galactose at terminal presented the ion at m/z 317.In the case of oligosaccharides with a fructose residue,characteristic ion of m/z 155 was produced.The conditions of stereoselective derivatizations and mass spectrometry were studied,in order to obtain a better reproducibility of the mass spectra.
