In this paper,crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata.The effects of crude monkshood polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microca...In this paper,crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata.The effects of crude monkshood polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microcalorimetry.The power-time curves of the bacterial growth at various concentrations (c) of crude monkshood polysaccharide were plotted with a TAM air isothermal microcalorimeter at 37 °C.The growth rate constant (μ),inhibitory ratio (I),peak-height (Pm),and peak-time (tm) were calculated.From the data,the relationship between μ and c also was established.The growth rate constant μ decreased with the increasing concentrations of crude monkshood polysaccharide.Moreover,Pm reduced and tm increased with increasing concentrations.The experimental results revealed that crude monkshood polysaccharide had inhibitory activity towards S.aureus and E.coli.Results obtained from our study strongly suggest that microcalorimetry is a fast,simple,and more sensitive technology that can be easily performed to study the effect of drugs on bacteria.展开更多
[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug d...[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug dose, which was the minimum dose causing 100% lethally rate in the preliminary test, was diluted into seven con- centrations in formal test. Then every mouse was orally administrated with 0.04 ml/g of the monkshood solution at single time for 7 d. Median lethal dose （LD50） and 95% confidence limit were calculated by the improved Karber method formulas. [Result] LD50 of monkshood ethanol extract was 230.12 mg/kg and 95% confidence limit of LD50 was 80.39-658.57 mg/kg. [Conclusion] Crude monkshood alcohol extract can quickly induce acute toxicity in mice.展开更多
基金Project supported by the Natural Science Foundation of Shandong Province (No. Y2007C141)the Independent Innovation Foundation of Shandong University (No. 2010TS041)the Award Program for Outstanding Young Scientist of Shandong Province (No. 2007BS02002), China
文摘In this paper,crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata.The effects of crude monkshood polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microcalorimetry.The power-time curves of the bacterial growth at various concentrations (c) of crude monkshood polysaccharide were plotted with a TAM air isothermal microcalorimeter at 37 °C.The growth rate constant (μ),inhibitory ratio (I),peak-height (Pm),and peak-time (tm) were calculated.From the data,the relationship between μ and c also was established.The growth rate constant μ decreased with the increasing concentrations of crude monkshood polysaccharide.Moreover,Pm reduced and tm increased with increasing concentrations.The experimental results revealed that crude monkshood polysaccharide had inhibitory activity towards S.aureus and E.coli.Results obtained from our study strongly suggest that microcalorimetry is a fast,simple,and more sensitive technology that can be easily performed to study the effect of drugs on bacteria.
基金Supported by the National Natural Science Foundation of China (31201951)~~
文摘[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug dose, which was the minimum dose causing 100% lethally rate in the preliminary test, was diluted into seven con- centrations in formal test. Then every mouse was orally administrated with 0.04 ml/g of the monkshood solution at single time for 7 d. Median lethal dose （LD50） and 95% confidence limit were calculated by the improved Karber method formulas. [Result] LD50 of monkshood ethanol extract was 230.12 mg/kg and 95% confidence limit of LD50 was 80.39-658.57 mg/kg. [Conclusion] Crude monkshood alcohol extract can quickly induce acute toxicity in mice.
