T-2 toxin,an omnipresent environmental contaminant,poses a serious risk to the health of humans and animals due to its pronounced cardiotoxicity.This study aimed to elucidate the molecular mechanism of cardiac tissue ...T-2 toxin,an omnipresent environmental contaminant,poses a serious risk to the health of humans and animals due to its pronounced cardiotoxicity.This study aimed to elucidate the molecular mechanism of cardiac tissue damage by T-2 toxin.Twenty-four male Sprague-Dawley rats were orally administered T-2 toxin through gavage for 12 weeks at the dose of 0,10,and 100 nanograms per gram body weight per day(ng/(g·day)),respectively.Morphological,pathological,and ultrastructural alterations in cardiac tissue were meticulously examined.Non-targeted metabolomics analysis was employed to analyze alterations in cardiac metabolites.The expression of the Sirt3/FoxO3α/MnSOD signaling pathway and the level of oxidative stress markers were detected.The results showed that exposure to T-2 toxin elicited myocardial tissue disorders,interstitial hemorrhage,capillary dilation,and fibrotic damage.Mitochondria were markedly impaired,including swelling,fusion,matrix degradation,and membrane damage.Metabonomics analysis unveiled that T-2 toxin could cause alterations in cardiacmetabolic profiles as well as in the Sirt3/FoxO3α/MnSOD signaling pathway.T-2 toxin could inhibit the expressions of the signaling pathway and elevate the level of oxidative stress.In conclusion,the T-2 toxin probably induces cardiac fibrotic impairment by affecting amino acid and choline metabolism as well as up-regulating oxidative stress mediated by the Sirt3/FoxO3α/MnSOD signaling pathway.This study is expected to provide targets for preventing and treating T-2 toxin-induced cardiac fibrotic injury.展开更多
为阐明植物的线粒体MnSOD在逆境适应性反应中的作用,本研究利用分子生物学技术,以拟南芥Mn-SOD基因上游序列构建其反义序列表达载体,转化拟南芥获得转基因植株。经Northern b lot鉴定发现转基因拟南芥MnSOD mRNA水平降低,NBT法检测转基...为阐明植物的线粒体MnSOD在逆境适应性反应中的作用,本研究利用分子生物学技术,以拟南芥Mn-SOD基因上游序列构建其反义序列表达载体,转化拟南芥获得转基因植株。经Northern b lot鉴定发现转基因拟南芥MnSOD mRNA水平降低,NBT法检测转基因拟南芥MnSOD活性下降,表明该反义DNA序列对MnSOD基因表达具有明显的抑制效果。展开更多
基金supported by the National Natural Science Foundation of China(No.81872567).
文摘T-2 toxin,an omnipresent environmental contaminant,poses a serious risk to the health of humans and animals due to its pronounced cardiotoxicity.This study aimed to elucidate the molecular mechanism of cardiac tissue damage by T-2 toxin.Twenty-four male Sprague-Dawley rats were orally administered T-2 toxin through gavage for 12 weeks at the dose of 0,10,and 100 nanograms per gram body weight per day(ng/(g·day)),respectively.Morphological,pathological,and ultrastructural alterations in cardiac tissue were meticulously examined.Non-targeted metabolomics analysis was employed to analyze alterations in cardiac metabolites.The expression of the Sirt3/FoxO3α/MnSOD signaling pathway and the level of oxidative stress markers were detected.The results showed that exposure to T-2 toxin elicited myocardial tissue disorders,interstitial hemorrhage,capillary dilation,and fibrotic damage.Mitochondria were markedly impaired,including swelling,fusion,matrix degradation,and membrane damage.Metabonomics analysis unveiled that T-2 toxin could cause alterations in cardiacmetabolic profiles as well as in the Sirt3/FoxO3α/MnSOD signaling pathway.T-2 toxin could inhibit the expressions of the signaling pathway and elevate the level of oxidative stress.In conclusion,the T-2 toxin probably induces cardiac fibrotic impairment by affecting amino acid and choline metabolism as well as up-regulating oxidative stress mediated by the Sirt3/FoxO3α/MnSOD signaling pathway.This study is expected to provide targets for preventing and treating T-2 toxin-induced cardiac fibrotic injury.
文摘以茄子品种‘禾线’为材料,采用同源克隆方法从茄子中克隆获得一个MnSOD基因,其开放阅读框全长为687 bp,编码228个氨基酸,将其命名为SmMnSOD。序列分析表明,SmMnSOD与辣椒MnSOD序列相似性达到91%,与番茄MnSOD序列相似性达到84%。预测该蛋白质分子量为25.63 k Da,蛋白等电点为8.46。亚细胞定位结果显示,MnSOD蛋白定位于线粒体。实时荧光定量PCR表明,SmMnSOD在不同组织器官中均有表达,但在叶片中表达量最多,其次为花瓣和根,果肉中最少。在Na Cl胁迫下,SmMnSOD表达量呈现先上升后下降趋势;聚乙二醇胁迫抑制了SmMnSOD的表达;外源脱落酸胁迫下,SmMnSOD的表达量是波动变化的;低温胁迫极显著地抑制了SmMnSOD的表达。实验结果证明,SmMnSOD主要在茄子叶片、花瓣和根中表达,在线粒体中发挥功效,可能与茄子抵御渗透性胁迫和干旱胁迫相关。
文摘为阐明植物的线粒体MnSOD在逆境适应性反应中的作用,本研究利用分子生物学技术,以拟南芥Mn-SOD基因上游序列构建其反义序列表达载体,转化拟南芥获得转基因植株。经Northern b lot鉴定发现转基因拟南芥MnSOD mRNA水平降低,NBT法检测转基因拟南芥MnSOD活性下降,表明该反义DNA序列对MnSOD基因表达具有明显的抑制效果。
