A recent study revealed a novel mechanism by which radiotherapy promotes distant metastatic growth by inducing amphiregulin(AREG)secretion from tumor cells.1 Specifically,tumor-derived AREG reprograms mononuclear phag...A recent study revealed a novel mechanism by which radiotherapy promotes distant metastatic growth by inducing amphiregulin(AREG)secretion from tumor cells.1 Specifically,tumor-derived AREG reprograms mononuclear phagocytes(MNPs)into an immunosuppressive phenotype by activating the epidermal growth factor receptor(EGFR)on their surface.Concurrently,AREG upregulates CD47 expression on tumor cells via the signal transducer and activator of transcription 3(STAT3)pathway,which inhibits phagocytic function and facilitates metastatic progression.This discovery challenges the conventional understanding of the systemic effects of radiotherapy.It also identifies combinatorial therapeutic targets that promise an improvement in radiotherapy efficacy and enable the formulation of personalized treatment strategies.展开更多
Small heat shock proteins(sHSPs)act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress,especially heat shock,but the mechanism is not clear.To study the function of sHS...Small heat shock proteins(sHSPs)act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress,especially heat shock,but the mechanism is not clear.To study the function of sHSPs in Lenzites gibbosa,a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees,under temperature stress,L.gibbosa mycelia were grown at 25℃ for 9 d,treated at 33℃ for 15,30,60,and 120 min before sequencing the transcriptomes.From among 32 heat shock protein(HSP)genes found in the screen of the transcriptome data,a highly expressed gene was cloned and named Lghsp17.4.RT-qPCR was used to analyze the expression of the gene Lghsp17.4 under heat shock and dye stress.Both treatments induced higher expression of Lghsp17.4 at the transcriptional level,indicating that Lghsp17.4 might function in the response to heat stress and dye degradation.We previously found that L.gibbosa generally had a heat shock reaction(HSR)during degradation of aromatic compounds,and HSPs were always produced with manganese peroxidases(MnPs)and other lignin-degrading enzymes.Therefore,we measured the activity of MnPs in L.gibbosa after 33℃ heat shock to analyze the relationship between MnPs expression and Lghsp17.4 expression.Heat shocks of 0–30 min increased MnPs activity,and the change in MnPs activity were closely positively correlated with the expression levels of Lghsp17.4 over time,indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in L.gibbosa.Thus,LgHSP17.4 might have a positive regulatory effect on the HSR in L.gibbosa and be a critical component of a stress resistance mechanism.展开更多
Objectives:Due to systematic side effects,there is a growing interest in nanoparticle formulation of anticancer drugs.Here,we aimed to synthesize poly(styrene-alt-maleic anhydride)cross-linked by melamine(PSMA/Me)and ...Objectives:Due to systematic side effects,there is a growing interest in nanoparticle formulation of anticancer drugs.Here,we aimed to synthesize poly(styrene-alt-maleic anhydride)cross-linked by melamine(PSMA/Me)and coated with magnetite nanoparticles(MNPs)PSMA/Me/Fe_(3)O_(4).In addition,we aimed to load paclitaxel(PTX)into PSMA/Me/Fe_(3)O_(4)for drug delivery and anticancer investigations.Methods:Novel PSMA/Me was synthesized via free radical copolymerization,coated with Fe_(3)O_(4),and then used as a transporter for PTX delivery.Fabricated copolymer was characterized using SEM,TGA,and XRD techniques.Drug release rate and loading efficiency were investigated.Human ovarian cancer cells(Skov-3)and breast cancer cells(MCF-7 cells)were incubated with the serial concentration of either free PTX or PSMA/Me/Fe_(3)O_(4)/PTX for cell viability and IC_(50)analysis for 24 and 48 h.Results:Characterization methods confirmed PSMA/Me copolymer formation.The results showed a significant encapsulation efficiency of 83%.The drug release analysis exhibited that PSMA/Me/Fe_(3)O_(4)/PTX may be considered pH-sensitive nanocarriers.PSMA/Me/Fe_(3)O_(4)/PTX reduced cell viability both dose and time-dependently(p<0.05).IC50 values of PSMA/Me/Fe_(3)O_(4)/PTX were low when compared to free PTX either 24 or 48 h post-treatment.Conclusions:Our results indicated that PSMA/Me/Fe_(3)O_(4)/PTX was more cytotoxic than PTX in both cancer cells.Findings indicated the potential of PSMA/Me/Fe_(3)O_(4)/PTX as an anticancer nanocarrier system.展开更多
The biocompatibility and biodistribution of magnetic nanoparticles(MNPs)in vivo are essential to ensure their safely clinical application.We have studied these aspects with our 3-aminopropyltriethoxysilanecoated magne...The biocompatibility and biodistribution of magnetic nanoparticles(MNPs)in vivo are essential to ensure their safely clinical application.We have studied these aspects with our 3-aminopropyltriethoxysilanecoated magnetic nanoparticles(APTS-MNPs)formulation,which can be used as magnetic induction hyperthermia media.Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice.Liver and kidney functions were tested.Heart,liver,spleen,lung,kidney,testis,and brain were sectioned for pathological analysis.Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues.Serum showed an increase in AST and LDH following APTS-MNPs injection.Histological analyses of selected tissues showed no obvious abnormal changes.In conclusion,APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application.展开更多
Curcumin is a natural polyphenol that is used in various traditional medicines.However,its inherent properties,such as its rapid degradation and metabolism,low bioavailability,and short half-life,are serious problems ...Curcumin is a natural polyphenol that is used in various traditional medicines.However,its inherent properties,such as its rapid degradation and metabolism,low bioavailability,and short half-life,are serious problems that must be resolved.To this end,a drug carrier incorporating natural magnetic cores in a zeolite framework was developed and applied to the loading of curcumin in ethanol solutions.In this system,curcumin is encapsulated in a zeolite Na(ZNA)magnetic core–shell structure(Fe@Si/ZNA),which can be easily synthesized using an in situ method.Synthesis of Fe_(3)O_(4) nanoparticles was carried out from natural materials using a co-precipitation method.Analysis of the prepared magnetic core–shell structures and composites was carried out using vibrating-sample magnetometery,Fourier transform infrared spectroscopy,transmission electron microscopy,and x-ray diffraction.The cumulative loading of curcumin in the ZNA composite with 9%nanoparticles was found to reach 90.70%with a relatively long half-life of 32.49 min.Stability tests of curcumin loading in the composite showed that adding magnetic particles to the zeolite framework also increased the stability of the composite structure.Adsorption kinetics and isotherm studies also found that the system follows the pseudo-second-order and Langmuir isotherm models.展开更多
研究了N-氨乙基-γ-氨丙基三甲氧基硅烷在水溶液中以单层形式负载于磁铁矿纳米颗粒表面的方法,并研究了单分子层硅烷负载磁铁矿纳米颗粒吸附剂(monolayer of silane on magnetite nanoparticles,MSMNPs)的除磷性能.结果表明,在低浓度下...研究了N-氨乙基-γ-氨丙基三甲氧基硅烷在水溶液中以单层形式负载于磁铁矿纳米颗粒表面的方法,并研究了单分子层硅烷负载磁铁矿纳米颗粒吸附剂(monolayer of silane on magnetite nanoparticles,MSMNPs)的除磷性能.结果表明,在低浓度下(平衡浓度小于300 mg·L^(-1))硅烷在磁铁矿纳米颗粒表面产生单层吸附,而反应温度90℃以上或离子强度0.1 mol·L^(-1)NaCl以上可以使单层硅烷在磁铁矿纳米颗粒表面的覆盖度达到~100%.FTIR和XPS图谱显示负载的硅烷以化学键的形式与磁铁矿纳米颗粒表面相结合.单层硅烷负载不引起磁性强度的明显变化.MSMNPs对磷的吸附等温线更符合Langmuir模型,拟合得到的最大吸附量为7.59 mg·g^(-1).由于磷吸附位位于MSMNPs的最外面,磷的吸附及脱附均很快,30 min内达到90%以上,1 h内达到平衡.因此,MSMNPs是一种易分离因而可反复使用,并且可快速吸附与脱附污染物的新型吸附剂.展开更多
基金supported by the National Natural Science Foundation of China(32000799)the Natural Science Foundation of Zhejiang Province,China(D25H300005)+1 种基金Zhejiang Provincial Traditional Chinese Medicine Science and Technology Project(GZY-KJS-ZJ-2025-072)Wenzhou Basic Scientific Research Project(Y20240675).
文摘A recent study revealed a novel mechanism by which radiotherapy promotes distant metastatic growth by inducing amphiregulin(AREG)secretion from tumor cells.1 Specifically,tumor-derived AREG reprograms mononuclear phagocytes(MNPs)into an immunosuppressive phenotype by activating the epidermal growth factor receptor(EGFR)on their surface.Concurrently,AREG upregulates CD47 expression on tumor cells via the signal transducer and activator of transcription 3(STAT3)pathway,which inhibits phagocytic function and facilitates metastatic progression.This discovery challenges the conventional understanding of the systemic effects of radiotherapy.It also identifies combinatorial therapeutic targets that promise an improvement in radiotherapy efficacy and enable the formulation of personalized treatment strategies.
基金supported by the Fundamental Research Funds for the Central Universities(Grant No.:2572016AA04)Northeast Asia Biodiversity Research Center Double First class Funds(Grant No.:411146030416 and No.:411147021003).
文摘Small heat shock proteins(sHSPs)act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress,especially heat shock,but the mechanism is not clear.To study the function of sHSPs in Lenzites gibbosa,a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees,under temperature stress,L.gibbosa mycelia were grown at 25℃ for 9 d,treated at 33℃ for 15,30,60,and 120 min before sequencing the transcriptomes.From among 32 heat shock protein(HSP)genes found in the screen of the transcriptome data,a highly expressed gene was cloned and named Lghsp17.4.RT-qPCR was used to analyze the expression of the gene Lghsp17.4 under heat shock and dye stress.Both treatments induced higher expression of Lghsp17.4 at the transcriptional level,indicating that Lghsp17.4 might function in the response to heat stress and dye degradation.We previously found that L.gibbosa generally had a heat shock reaction(HSR)during degradation of aromatic compounds,and HSPs were always produced with manganese peroxidases(MnPs)and other lignin-degrading enzymes.Therefore,we measured the activity of MnPs in L.gibbosa after 33℃ heat shock to analyze the relationship between MnPs expression and Lghsp17.4 expression.Heat shocks of 0–30 min increased MnPs activity,and the change in MnPs activity were closely positively correlated with the expression levels of Lghsp17.4 over time,indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in L.gibbosa.Thus,LgHSP17.4 might have a positive regulatory effect on the HSR in L.gibbosa and be a critical component of a stress resistance mechanism.
文摘Objectives:Due to systematic side effects,there is a growing interest in nanoparticle formulation of anticancer drugs.Here,we aimed to synthesize poly(styrene-alt-maleic anhydride)cross-linked by melamine(PSMA/Me)and coated with magnetite nanoparticles(MNPs)PSMA/Me/Fe_(3)O_(4).In addition,we aimed to load paclitaxel(PTX)into PSMA/Me/Fe_(3)O_(4)for drug delivery and anticancer investigations.Methods:Novel PSMA/Me was synthesized via free radical copolymerization,coated with Fe_(3)O_(4),and then used as a transporter for PTX delivery.Fabricated copolymer was characterized using SEM,TGA,and XRD techniques.Drug release rate and loading efficiency were investigated.Human ovarian cancer cells(Skov-3)and breast cancer cells(MCF-7 cells)were incubated with the serial concentration of either free PTX or PSMA/Me/Fe_(3)O_(4)/PTX for cell viability and IC_(50)analysis for 24 and 48 h.Results:Characterization methods confirmed PSMA/Me copolymer formation.The results showed a significant encapsulation efficiency of 83%.The drug release analysis exhibited that PSMA/Me/Fe_(3)O_(4)/PTX may be considered pH-sensitive nanocarriers.PSMA/Me/Fe_(3)O_(4)/PTX reduced cell viability both dose and time-dependently(p<0.05).IC50 values of PSMA/Me/Fe_(3)O_(4)/PTX were low when compared to free PTX either 24 or 48 h post-treatment.Conclusions:Our results indicated that PSMA/Me/Fe_(3)O_(4)/PTX was more cytotoxic than PTX in both cancer cells.Findings indicated the potential of PSMA/Me/Fe_(3)O_(4)/PTX as an anticancer nanocarrier system.
基金supported by the National Natural Science Foundation of China (81071885)
文摘The biocompatibility and biodistribution of magnetic nanoparticles(MNPs)in vivo are essential to ensure their safely clinical application.We have studied these aspects with our 3-aminopropyltriethoxysilanecoated magnetic nanoparticles(APTS-MNPs)formulation,which can be used as magnetic induction hyperthermia media.Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice.Liver and kidney functions were tested.Heart,liver,spleen,lung,kidney,testis,and brain were sectioned for pathological analysis.Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues.Serum showed an increase in AST and LDH following APTS-MNPs injection.Histological analyses of selected tissues showed no obvious abnormal changes.In conclusion,APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application.
基金funding from the Ministry of Education,Culture,Research,and Technology,Indonesia,through the PDKN Research Grant with Contract No.041/E5/PG.02.00.PL/2023.
文摘Curcumin is a natural polyphenol that is used in various traditional medicines.However,its inherent properties,such as its rapid degradation and metabolism,low bioavailability,and short half-life,are serious problems that must be resolved.To this end,a drug carrier incorporating natural magnetic cores in a zeolite framework was developed and applied to the loading of curcumin in ethanol solutions.In this system,curcumin is encapsulated in a zeolite Na(ZNA)magnetic core–shell structure(Fe@Si/ZNA),which can be easily synthesized using an in situ method.Synthesis of Fe_(3)O_(4) nanoparticles was carried out from natural materials using a co-precipitation method.Analysis of the prepared magnetic core–shell structures and composites was carried out using vibrating-sample magnetometery,Fourier transform infrared spectroscopy,transmission electron microscopy,and x-ray diffraction.The cumulative loading of curcumin in the ZNA composite with 9%nanoparticles was found to reach 90.70%with a relatively long half-life of 32.49 min.Stability tests of curcumin loading in the composite showed that adding magnetic particles to the zeolite framework also increased the stability of the composite structure.Adsorption kinetics and isotherm studies also found that the system follows the pseudo-second-order and Langmuir isotherm models.
