A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral...A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral RNAs were released and were detected by RT-PCR.CGMMV could be detected in as less as 10 ng watermelon leaf materials.Compared with normal RT-PCR,the method decreased the inhibitors of plant material and steps for extracting RNA,and also increased the sensitivity of RT-PCR detection in less time.The method is simple and suitable for quick detection of plant virus in a large number of samples.展开更多
文摘A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral RNAs were released and were detected by RT-PCR.CGMMV could be detected in as less as 10 ng watermelon leaf materials.Compared with normal RT-PCR,the method decreased the inhibitors of plant material and steps for extracting RNA,and also increased the sensitivity of RT-PCR detection in less time.The method is simple and suitable for quick detection of plant virus in a large number of samples.
