AIM: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinicalpathological parameters of patients with gallbladder carcinoma.METHODS: Carcinomas (n=45) and polypoi...AIM: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinicalpathological parameters of patients with gallbladder carcinoma.METHODS: Carcinomas (n=45) and polypoid lesions (n=15) of the gallbladder were studied for the expression of MMP-2 and TIMP-2 protein by immunohistochemical avidin-biotin-complex method and image analysis. Clinicalpathological data of patients with gallbladder carcinoma such as histological type, grade of differentiation, level of infiltration, liver invasion and lymph node involvement, etc, were recorded.RESULTS: There was significant difference between the average level (1.123±0.108 VS 1.030±0.054, P=0.002) of MMP-2, the ratio (1.050±0.013 VS0.937±0.078, P=0.003) of MMP-2/TIMP-2 in gallbladder carcinomas and in polypoid lesions of the gallbladder. Significant difference was found between the expression of MMP-2 in early stage and advanced tumors, but there was no correlation between MMP-2 protein expression and histological type, differentiation degree, infiltration level, lymph node involvement or liver invasion. Although no difference was observed between TIMP-2 expression and histological type or differentiation degree, signific ant difference was found between TIMP-2 expression and different Nevin stage, infiltration level, local lymph node involvement or liver invasion (1.168±0.067 VS1.048±0.075, 1.170±0.062 vs 1.039±0.06g, 1.039±0.076 VS1.147±0.083, 1.048±0.074 vs 1.103±0.095, P＜0.05). MMP-2/TIMP-2 ratio did not correlate with histological type, grade of differentiation and liver invasion, but significant differences were found between MMP-2/TIMP-2 ratio and different Nevin stage, infiltration level and lymph node involvement in patients with carcinoma of gallbladder.CONCLUSION: TIMP-2 and MMP-2/TIMP-2 ratio could reflect more accurately biological characteristic of gallbladder carcinoma and MMP-2/TIMP-2 ratio might be a new significant marker in early diagnosis, in the judgment of invasion or metastasis and the estimate of prognosis in patients with gallbladder carcinomas.展开更多
目的:探究子痫前期患者血清胎盘蛋白-13(Placental protein 13,PP13)、基质金属蛋白酶抑制因子2(Tissue inhibitor of metalloproteinase,TIMP-2)、缺氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)水平与胎儿生长受限的相关性...目的:探究子痫前期患者血清胎盘蛋白-13(Placental protein 13,PP13)、基质金属蛋白酶抑制因子2(Tissue inhibitor of metalloproteinase,TIMP-2)、缺氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)水平与胎儿生长受限的相关性及临床意义。方法:选取2021年9月至2023年9月期间本院收治的94例子痫前期孕妇作为研究对象。根据孕妇分娩后胎儿的体重情况评估胎儿是否发生胎儿生长受限(Fetal growth restriction,FGR),将研究对象分为FGR组(n=31)和非FGR组(n=63)。另选取同期内的94例健康孕妇作为研究对象的对照组。对比三组血清PP13、TIMP-2、HIF-1α水平。分析血清PP13、TIMP-2、HIF-1α水平与FGR的相关性和独立影响因素。分析各指标对子痫前期患者FGR发生的预测价值。结果:FGR组的血清PP13水平明显低于非FGR组和对照组,非FGR组血清PP13水平低于明显对照组(P<0.05)。FGR组的血清TIMP-2和HIF-1α水平明显高于非FGR组和对照组,非FGR组的血清TIMP-2和HIF-1α水平明显高于对照组(P<0.05)。血清PP13水平与FGR发生呈负相关,血清TIMP-2、HIF-1α水平与FGR发生呈正相关(P<0.05)。血清PP13是FGR发生的独立保护因素,血清TIMP-2、HIF-1α是FGR发生的独立危险因素(P<0.05)。血清PP13、TIMP-2、HIF-1α水平联合预测FGR发生的曲线下面积(Area under the curve,AUC)明显大于各血清指标的单独预测(P<0.05)。结论:血清PP13、TIMP-2、HIF-1α水平与子痫前期患者FGR发生关系密切,各指标联合检测可为预测FGR发生提供有效参考。展开更多
Objective:To investigate the effects of electroacupuncture(EA)on the blood-brain barrier permeability and the regulation of matrix metalloproteinases(MMPs)in the mice with Parkinson’s disease(PD).Methods:Forty-eight ...Objective:To investigate the effects of electroacupuncture(EA)on the blood-brain barrier permeability and the regulation of matrix metalloproteinases(MMPs)in the mice with Parkinson’s disease(PD).Methods:Forty-eight C57BL/6 mice were randomly assigned into the normal control(NC)group,the PD model(PD)group,the EA group and the EA+SB-3CT inhibitor group(EA+SB-3CT),with 12 mice in each group.In this experiment,the PD model was established by intragastric administration(IG)with rotenone for 4 wk in the PD group,EA group and EA+SB-3CT group.In the EA+SB-3CT group,1 h after IG with rotenone,the mice were intraperitoneally injected with MMP-2/9 inhibitor,SB-3CT(25 mg/kg/d).After successfully modeled,in the EA group and EA+SB-3CT group,EA was conducted at“Fengfu(GV16)”and bilateral“Taichong(LR3)”and“Zusanli(ST36)”,at 1 mA and 2 Hz for 30 min each time,once a day,for consecutive 2 wk.The behavioral changes of the mice were observed in each group using the open field test,the level of tyrosine hydroxylase(TH)in the substantia nigra was determined by immunohistochemistry,the permeability of the blood-brain barrier was detected by Evans blue staining,and the protein expression of ZO-1,ocludin,claudin-1,MMP-2 and MMP-9 in the substantia nigra was detected by Western blotting.Results:Compared with the NC group,the behavioral scores increased(P<0.05),while total time of locomotion,total distance and average speed were reduced(P<0.05)in the PD group.The expression of TH in the substantia nigra decreased(P<0.05),Evans blue level in the brain tissue increased(P<0.05),and the protein expression of ZO-1,occludin and claudin-1 was lower(P<0.05),whereas MMP-2 and MMP-9 expression was higher(P<0.05)in the PD group.Compared with the PD group,behavioral scores decreased(P<0.05),while the total time of locomotion,total distance and average speed increased(P<0.05)in the EA group.Additionally,TH expression in the substantia nigra was elevated(P<0.05),Evans blue level in the brain tissue was lower(P<0.05),the protein expression of ZO-1,occludin and claudin-1 was up-regulated(P<0.05),and MMP-2 and MMP-9 expression was down-regulated(P<0.05)in the EA group.Compared with the EA group,Evans blue level was reduced(P<0.05),the protein expression of ZO-1 and occludin was up-regulated(P<0.05),and MMP-2 and MMP-9 expression was further down-regulated(P<0.05)in the EA+SB-3CT group.Conclusion:EA can effectively ameliorate the motor dysfunction of PD mice,reduce the damage of dopaminergic neurons,and play a neuroprotective role.EA can effectively improve the blood–brain barrier permeability in PD mice by up-regulating the expression of tight junction proteins,ZO-1 and occludin,and down-regulating the expression of matrix metalloporteinases,MMP-2 and MMP-9.The neuroprotective role of EA may be obtained by improving the blood-brain barrier permeability mediated by MMP-2/9 pathway.展开更多
目的:应用肌性斜颈兔动物模型,探讨针刀治疗肌性斜颈的作用机制及意义。方法:无水酒精注射制备动物肌性斜颈兔子模型后,选取模型兔子48只,随机分为正常组、模型组、针刀组、按摩组,每组12只。分别给予各组对应治疗,治疗2个月后各组分别...目的:应用肌性斜颈兔动物模型,探讨针刀治疗肌性斜颈的作用机制及意义。方法:无水酒精注射制备动物肌性斜颈兔子模型后,选取模型兔子48只,随机分为正常组、模型组、针刀组、按摩组,每组12只。分别给予各组对应治疗,治疗2个月后各组分别取部分胸锁乳突肌制作标本,免疫组化法观察钙蛋白酶1(calpain-1)、分化抗原决定簇45(CD45)、基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶抑制因子2(tissue inhibitor of metalloproteinases-2,TIMP-2)在各组肌纤维中的表达情况。结果:与正常组比较,其余各组实验动物明显出现颈部活动受限、斜颈、头颈不对称症状,而治疗结束后,针刀组和按摩组实验动物颈部活动受限改善,无明显头颈不对称症状。calpain-1、CD45、TIMP-2的表达,与正常组比较,模型组、按摩组上调(P<0.01);与模型组比较,针刀组、按摩组下调(P<0.01);与针刀组比较,按摩组上调(P<0.01)。MMP-2表达,与正常组比较,模型组下调(P<0.01),与模型组比较,针刀组、按摩组上调(P<0.05)。结论:针刀治疗肌性斜颈效果显著,其作用机制可能与减少calpain-1、CD45、TIMP-2表达,增加MMP-2表达有关。展开更多
基金Scientific Research Foundation of the Railway Ministry,China,No.TDB99-69
文摘AIM: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinicalpathological parameters of patients with gallbladder carcinoma.METHODS: Carcinomas (n=45) and polypoid lesions (n=15) of the gallbladder were studied for the expression of MMP-2 and TIMP-2 protein by immunohistochemical avidin-biotin-complex method and image analysis. Clinicalpathological data of patients with gallbladder carcinoma such as histological type, grade of differentiation, level of infiltration, liver invasion and lymph node involvement, etc, were recorded.RESULTS: There was significant difference between the average level (1.123±0.108 VS 1.030±0.054, P=0.002) of MMP-2, the ratio (1.050±0.013 VS0.937±0.078, P=0.003) of MMP-2/TIMP-2 in gallbladder carcinomas and in polypoid lesions of the gallbladder. Significant difference was found between the expression of MMP-2 in early stage and advanced tumors, but there was no correlation between MMP-2 protein expression and histological type, differentiation degree, infiltration level, lymph node involvement or liver invasion. Although no difference was observed between TIMP-2 expression and histological type or differentiation degree, signific ant difference was found between TIMP-2 expression and different Nevin stage, infiltration level, local lymph node involvement or liver invasion (1.168±0.067 VS1.048±0.075, 1.170±0.062 vs 1.039±0.06g, 1.039±0.076 VS1.147±0.083, 1.048±0.074 vs 1.103±0.095, P＜0.05). MMP-2/TIMP-2 ratio did not correlate with histological type, grade of differentiation and liver invasion, but significant differences were found between MMP-2/TIMP-2 ratio and different Nevin stage, infiltration level and lymph node involvement in patients with carcinoma of gallbladder.CONCLUSION: TIMP-2 and MMP-2/TIMP-2 ratio could reflect more accurately biological characteristic of gallbladder carcinoma and MMP-2/TIMP-2 ratio might be a new significant marker in early diagnosis, in the judgment of invasion or metastasis and the estimate of prognosis in patients with gallbladder carcinomas.
文摘目的:探究子痫前期患者血清胎盘蛋白-13(Placental protein 13,PP13)、基质金属蛋白酶抑制因子2(Tissue inhibitor of metalloproteinase,TIMP-2)、缺氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)水平与胎儿生长受限的相关性及临床意义。方法:选取2021年9月至2023年9月期间本院收治的94例子痫前期孕妇作为研究对象。根据孕妇分娩后胎儿的体重情况评估胎儿是否发生胎儿生长受限(Fetal growth restriction,FGR),将研究对象分为FGR组(n=31)和非FGR组(n=63)。另选取同期内的94例健康孕妇作为研究对象的对照组。对比三组血清PP13、TIMP-2、HIF-1α水平。分析血清PP13、TIMP-2、HIF-1α水平与FGR的相关性和独立影响因素。分析各指标对子痫前期患者FGR发生的预测价值。结果:FGR组的血清PP13水平明显低于非FGR组和对照组,非FGR组血清PP13水平低于明显对照组(P<0.05)。FGR组的血清TIMP-2和HIF-1α水平明显高于非FGR组和对照组,非FGR组的血清TIMP-2和HIF-1α水平明显高于对照组(P<0.05)。血清PP13水平与FGR发生呈负相关,血清TIMP-2、HIF-1α水平与FGR发生呈正相关(P<0.05)。血清PP13是FGR发生的独立保护因素,血清TIMP-2、HIF-1α是FGR发生的独立危险因素(P<0.05)。血清PP13、TIMP-2、HIF-1α水平联合预测FGR发生的曲线下面积(Area under the curve,AUC)明显大于各血清指标的单独预测(P<0.05)。结论:血清PP13、TIMP-2、HIF-1α水平与子痫前期患者FGR发生关系密切,各指标联合检测可为预测FGR发生提供有效参考。
基金Supported by National Natural Science Foundation of China:No.8147378。
文摘Objective:To investigate the effects of electroacupuncture(EA)on the blood-brain barrier permeability and the regulation of matrix metalloproteinases(MMPs)in the mice with Parkinson’s disease(PD).Methods:Forty-eight C57BL/6 mice were randomly assigned into the normal control(NC)group,the PD model(PD)group,the EA group and the EA+SB-3CT inhibitor group(EA+SB-3CT),with 12 mice in each group.In this experiment,the PD model was established by intragastric administration(IG)with rotenone for 4 wk in the PD group,EA group and EA+SB-3CT group.In the EA+SB-3CT group,1 h after IG with rotenone,the mice were intraperitoneally injected with MMP-2/9 inhibitor,SB-3CT(25 mg/kg/d).After successfully modeled,in the EA group and EA+SB-3CT group,EA was conducted at“Fengfu(GV16)”and bilateral“Taichong(LR3)”and“Zusanli(ST36)”,at 1 mA and 2 Hz for 30 min each time,once a day,for consecutive 2 wk.The behavioral changes of the mice were observed in each group using the open field test,the level of tyrosine hydroxylase(TH)in the substantia nigra was determined by immunohistochemistry,the permeability of the blood-brain barrier was detected by Evans blue staining,and the protein expression of ZO-1,ocludin,claudin-1,MMP-2 and MMP-9 in the substantia nigra was detected by Western blotting.Results:Compared with the NC group,the behavioral scores increased(P<0.05),while total time of locomotion,total distance and average speed were reduced(P<0.05)in the PD group.The expression of TH in the substantia nigra decreased(P<0.05),Evans blue level in the brain tissue increased(P<0.05),and the protein expression of ZO-1,occludin and claudin-1 was lower(P<0.05),whereas MMP-2 and MMP-9 expression was higher(P<0.05)in the PD group.Compared with the PD group,behavioral scores decreased(P<0.05),while the total time of locomotion,total distance and average speed increased(P<0.05)in the EA group.Additionally,TH expression in the substantia nigra was elevated(P<0.05),Evans blue level in the brain tissue was lower(P<0.05),the protein expression of ZO-1,occludin and claudin-1 was up-regulated(P<0.05),and MMP-2 and MMP-9 expression was down-regulated(P<0.05)in the EA group.Compared with the EA group,Evans blue level was reduced(P<0.05),the protein expression of ZO-1 and occludin was up-regulated(P<0.05),and MMP-2 and MMP-9 expression was further down-regulated(P<0.05)in the EA+SB-3CT group.Conclusion:EA can effectively ameliorate the motor dysfunction of PD mice,reduce the damage of dopaminergic neurons,and play a neuroprotective role.EA can effectively improve the blood–brain barrier permeability in PD mice by up-regulating the expression of tight junction proteins,ZO-1 and occludin,and down-regulating the expression of matrix metalloporteinases,MMP-2 and MMP-9.The neuroprotective role of EA may be obtained by improving the blood-brain barrier permeability mediated by MMP-2/9 pathway.
文摘目的:应用肌性斜颈兔动物模型,探讨针刀治疗肌性斜颈的作用机制及意义。方法:无水酒精注射制备动物肌性斜颈兔子模型后,选取模型兔子48只,随机分为正常组、模型组、针刀组、按摩组,每组12只。分别给予各组对应治疗,治疗2个月后各组分别取部分胸锁乳突肌制作标本,免疫组化法观察钙蛋白酶1(calpain-1)、分化抗原决定簇45(CD45)、基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶抑制因子2(tissue inhibitor of metalloproteinases-2,TIMP-2)在各组肌纤维中的表达情况。结果:与正常组比较,其余各组实验动物明显出现颈部活动受限、斜颈、头颈不对称症状,而治疗结束后,针刀组和按摩组实验动物颈部活动受限改善,无明显头颈不对称症状。calpain-1、CD45、TIMP-2的表达,与正常组比较,模型组、按摩组上调(P<0.01);与模型组比较,针刀组、按摩组下调(P<0.01);与针刀组比较,按摩组上调(P<0.01)。MMP-2表达,与正常组比较,模型组下调(P<0.01),与模型组比较,针刀组、按摩组上调(P<0.05)。结论:针刀治疗肌性斜颈效果显著,其作用机制可能与减少calpain-1、CD45、TIMP-2表达,增加MMP-2表达有关。
