[ Objective] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure. The study will provide a theoretical basis for breeding of colored mink. ...[ Objective] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure. The study will provide a theoretical basis for breeding of colored mink. [Method] The exon 1 and its 5＇-flanking sequences of MLPHgene were amplified from minks. SignalP 3.0 Server, ClustalX 1.83, Mega 4.1, TMHM 2.0, ProtComp v6.0 and other software were used for sequence analysis. [ Result] A 1 029-bp fragment was amplified with the expected size. As calculated with the Mega 4.1, the minks had the nearest genetic distance with rattus （0.540 5） and the farthest with human （0.655 2）. Phylogenetic tree of mink was constructed. [ Conclusion] The mink MLPH gene encodes a secretory protein.展开更多
Automatic detection of a designated building area(DBA)is a research hotspot in the field of target detection using remote sensing images.Target detection is urgently needed for tasks such as illegal building monitorin...Automatic detection of a designated building area(DBA)is a research hotspot in the field of target detection using remote sensing images.Target detection is urgently needed for tasks such as illegal building monitoring,dynamic land use monitoring,antiterrorism efforts,and military reconnaissance.The existing detection methods generally have low efficiency and poor detection accuracy due to the large size and complexity of remote sensing scenes.To address the problems of the current detection methods,this paper presents a DBA detection method that uses hierarchical structural constraints in remote sensing images.Our method was conducted in two main stages.(1)During keypoint generation,we proposed a screening method based on structural pattern descriptors.The local pattern feature of the initial keypoints was described by a multilevel local pattern histogram(MLPH)feature;then,we used one-class support vector machine(OC-SVM)merely to screen those building attribute keypoints.(2)To match the screened keypoints,we proposed a reliable DBA detection method based on matching the local structural similarities of the screened keypoints.We achieved precise keypoint matching by calculating the similarities of the local skeletal structures in the neighboring areas around the roughly matched keypoints to achieve DBA detection.We tested the proposed method on building area sets of different types and at different time phases.The experimental results show that the proposed method is both highly accurate and computationally efficient.展开更多
基金supported by the Starting Fund for Doctors of Liaoning Medical College
文摘[ Objective] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure. The study will provide a theoretical basis for breeding of colored mink. [Method] The exon 1 and its 5＇-flanking sequences of MLPHgene were amplified from minks. SignalP 3.0 Server, ClustalX 1.83, Mega 4.1, TMHM 2.0, ProtComp v6.0 and other software were used for sequence analysis. [ Result] A 1 029-bp fragment was amplified with the expected size. As calculated with the Mega 4.1, the minks had the nearest genetic distance with rattus （0.540 5） and the farthest with human （0.655 2）. Phylogenetic tree of mink was constructed. [ Conclusion] The mink MLPH gene encodes a secretory protein.
基金This work was supported in part by the National Natural Science Foundation of China(Grant No.61601006)the Beijing Natural Science Foundation(Grant No.4192021)the Equipment Pre-Research Foundation(Grant No.61404130312).
文摘Automatic detection of a designated building area(DBA)is a research hotspot in the field of target detection using remote sensing images.Target detection is urgently needed for tasks such as illegal building monitoring,dynamic land use monitoring,antiterrorism efforts,and military reconnaissance.The existing detection methods generally have low efficiency and poor detection accuracy due to the large size and complexity of remote sensing scenes.To address the problems of the current detection methods,this paper presents a DBA detection method that uses hierarchical structural constraints in remote sensing images.Our method was conducted in two main stages.(1)During keypoint generation,we proposed a screening method based on structural pattern descriptors.The local pattern feature of the initial keypoints was described by a multilevel local pattern histogram(MLPH)feature;then,we used one-class support vector machine(OC-SVM)merely to screen those building attribute keypoints.(2)To match the screened keypoints,we proposed a reliable DBA detection method based on matching the local structural similarities of the screened keypoints.We achieved precise keypoint matching by calculating the similarities of the local skeletal structures in the neighboring areas around the roughly matched keypoints to achieve DBA detection.We tested the proposed method on building area sets of different types and at different time phases.The experimental results show that the proposed method is both highly accurate and computationally efficient.
