Mitochondrial Rho-GTPase 1(MIRO1)is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis.In present study,we reported the crucial roles of MIRO1 in mammalian oocyte ...Mitochondrial Rho-GTPase 1(MIRO1)is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis.In present study,we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging.We found that MIRO1 expressed in mouse and porcine oocytes,and its expression decreased in aged mice.MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes,which could be rescued by exogenous MIRO1 supplementation.Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins,and MIRO1 regulated motor protein Dynein for microtubule-organizing centers(MTOCs)dynamics at germinal vesicle(GV)stage,which determined meiotic resumption.Furthermore,we found that MIRO1 regulated Aurora A and kinesin family member 11(KIF11)for meiotic spindle assembly in oocytes.Besides,MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1(DRP1),Parkin and lysosomal-associated membrane protein 2(LAMP2)for mitochondrial dynamics and mitophagy during oocyte meiosis.Taken together,our results suggested that MIRO1 played pivotal roles in meiotic resumption,spindle assembly and mitochondrial function in mouse and porcine oocytes,and its insufficiency might contribute to the oocyte maturation defects during aging.展开更多
Mesenchymal stem cells(MSCs)are gaining the spotlight in research due to their abundant sources,immune privileges,and ability to proliferate and differentiate.These cells provide invaluable resources for stem cell-bas...Mesenchymal stem cells(MSCs)are gaining the spotlight in research due to their abundant sources,immune privileges,and ability to proliferate and differentiate.These cells provide invaluable resources for stem cell-based therapy and present therapeutic opportunities in cell/tissue regeneration medicine.A growing evidence suggests that,mitochondria transfer from MSCs could rescue tissue degeneration caused by mitochondria damage.Although this emerging tissue regeneration treatment method brings hope for the treatment of mitochondria dysfunction related diseases,the necessary conditions and underlying mechanisms for mitochondrial transfer remain poorly understood.In this review,a large number of mitochondrial transfer phenomena between MSCs and recipient cells are summarized,with particular emphasis on the conditions and potential mechanisms of mitochondrial transfer from MSCs,so as to provide reference for the clinical application of MSCs transformation in the future.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1300502)the Fundamental Research Funds for the Central Universities of China(KYT2024002,KJJQ2024003,and RENCAI2024011)the National Natural Science Foundation of China(32370908).
文摘Mitochondrial Rho-GTPase 1(MIRO1)is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis.In present study,we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging.We found that MIRO1 expressed in mouse and porcine oocytes,and its expression decreased in aged mice.MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes,which could be rescued by exogenous MIRO1 supplementation.Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins,and MIRO1 regulated motor protein Dynein for microtubule-organizing centers(MTOCs)dynamics at germinal vesicle(GV)stage,which determined meiotic resumption.Furthermore,we found that MIRO1 regulated Aurora A and kinesin family member 11(KIF11)for meiotic spindle assembly in oocytes.Besides,MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1(DRP1),Parkin and lysosomal-associated membrane protein 2(LAMP2)for mitochondrial dynamics and mitophagy during oocyte meiosis.Taken together,our results suggested that MIRO1 played pivotal roles in meiotic resumption,spindle assembly and mitochondrial function in mouse and porcine oocytes,and its insufficiency might contribute to the oocyte maturation defects during aging.
基金supported by the Scientific Research Project of Tianjin Education Commission(2017KJ162).
文摘Mesenchymal stem cells(MSCs)are gaining the spotlight in research due to their abundant sources,immune privileges,and ability to proliferate and differentiate.These cells provide invaluable resources for stem cell-based therapy and present therapeutic opportunities in cell/tissue regeneration medicine.A growing evidence suggests that,mitochondria transfer from MSCs could rescue tissue degeneration caused by mitochondria damage.Although this emerging tissue regeneration treatment method brings hope for the treatment of mitochondria dysfunction related diseases,the necessary conditions and underlying mechanisms for mitochondrial transfer remain poorly understood.In this review,a large number of mitochondrial transfer phenomena between MSCs and recipient cells are summarized,with particular emphasis on the conditions and potential mechanisms of mitochondrial transfer from MSCs,so as to provide reference for the clinical application of MSCs transformation in the future.
