miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基...miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。展开更多
Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is suscepti...Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is susceptible to tropical race 4(TR4),an aggressive race of the pathogen which is of increasing concern worldwide.Previous studies have revealed that plant small RNAs(sRNAs)play crucial roles in the host response to pathogen infection.To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem,small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing(NGS)technology.A total of 112 discrete mature microRNAs(miRNAs)belonging to 22 known miRNA families were found across all constructed sRNA libraries.The expression of miR166,miR159 and miR156 was upregulated in TR4-inoculated samples as compared to mock-inoculated samples,while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples.Consistent with the sequencing data,qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples,but not in R1-inoculated samples.Considering Cavendish banana is resistant to R1 and susceptible to TR4,it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence.The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement.展开更多
文摘miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。
基金The NSFC-Yunnan province joint foundation (U1136603)the Knowledge Innovation Project of the Chinese Academy of Sciences (KSCX2-YW-N-067)+3 种基金the National Natural Science Foundation of China (30990244)Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry and the Young Academic and Technical Leader Raising Foundation of Yunnan Province(2008PY065)the Western Light Talent Culture Project of the Chinese Academy of Sciences(2010312D11035)the Yunnan Provincial Government through an innovation team programme
基金funded by the Accelerated Partnership Grant,Queensland Government(2014000652)Shulang Fei’s PhD programme is supported by the China Scholarship Council and the University of Queensland。
文摘Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is susceptible to tropical race 4(TR4),an aggressive race of the pathogen which is of increasing concern worldwide.Previous studies have revealed that plant small RNAs(sRNAs)play crucial roles in the host response to pathogen infection.To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem,small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing(NGS)technology.A total of 112 discrete mature microRNAs(miRNAs)belonging to 22 known miRNA families were found across all constructed sRNA libraries.The expression of miR166,miR159 and miR156 was upregulated in TR4-inoculated samples as compared to mock-inoculated samples,while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples.Consistent with the sequencing data,qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples,but not in R1-inoculated samples.Considering Cavendish banana is resistant to R1 and susceptible to TR4,it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence.The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement.
